Search PubMed⌕ Search

Biomedical subjects

S Mizuno

Publications and source records attributed to S Mizuno.

At least 631 records · Page 35Linked to original sources
PubMed1977

Numerical analysis of relationships among rapidly growing, scotochromogenic mycobacteria.

Numerical analysis of the relationships among rapidly growing, scotochromogenic mycobacteria was carried out; a dendrogram was prepared by a single linkage method and the separation between species was tested. The following were distinct species: Mycobacterium alichiense; mycobacterium rhodesiae;Mycobacterium chubuense; Mycobacterium obuense; Mycobacterium gilvum; mycobacterium duvalii; mycobacterium flavescens; and mycobacterium vaccae. Mycobacterium tokaiense was reduced to a synonym of M. chubense. Mycobacterium parafortuitum, Mycobacterium aurum and Mycobacterium neoaurum were considered to be subspecies of M. parafortuitum or part of a M. parafortuitum complex. The separation between species was tested by comparing the extent of overlap of one species with that of another. When two species showed no such overlap,they were regarded as distinct. The results of the test of separation agreed well with taxonomic relationships shown by the dendrogram. Characters useful for differentiating the species were found.

Mycobacterium↗
PubMed1976

[Frozen shoulder].

Explore the source record for details and available documents.

Exercise Therapy↗
PubMed1976

Energization of mitochondrial inner membranes caused by L-malate.

It was found that 0.06 mug antimycin A/mg mitochondrial protein, an amount sufficient to inhibit electron transfer between cytochromes b and c1 completely, fully reversed the oxidation of cytochrome a caused by L-malate in anaerobic mitochondria. The effect of L-malate on cytochrome a was insensitive to oligomycin, but all the uncouplers and detergents tested reversed the oxidation of cytochrome a caused by L-malate in anaerobic mitochondria. It was also found that addition of L-malate to anaerobic mitochondria, like addition of ATP, decreased the fluorescence of 1-anilinonaphthalene-8-sulphonate, and that subsequent addition of uncouplers reversed this effect. The effect of L-malate on the fluorescence of the dye was insensitive to oligomycin. The present findings suggest that addition of L-malate may cause energization of the mitochondrial inner membranes and that the oxidation of cytochrome a caused by L-malate in anaerobic mitochondria may result from an L-malate-induced, energy-linked reversal of electron transfer in site II.

Adenosine Triphosphate↗
PubMed1976

Interspecific "common" repetitive DNA sequences in salamanders of the genus Plethodon.

Intermediate repetitive sequences of Plethodon cinereus which comprised about 30% of the genomic DNA were isolated and iodinated with 125I. About 5% of the 125I-repetitive fraction hybridized with a large excess of DNA from P. dunni at Cot 20. About half of the 125I-DNA in the hybrids was resistant to extensive digestion with S-1 nuclease. The average molecular size of the S-1 nuclease-resistant fraction was about 100 nucleotide pairs. The melting temperature of the S-1 nuclease-resistant fraction was about 2 degrees lower than that of the corresponding fraction made with P. cinereus DNA. These results are taken to indicate the presence in the genomes of P. cinereus and P. dunni of evolutionarily stable "common" repetitive sequences. The average frequency of repetition of the common repetitive sequences is about 6,000 X in both species. The common repetitive fraction is also present in the genomes of other species of Plethodon, although the general populations of intermediate repetitive sequences are markedly different from one species to another. The cinereus--dunni common repetitive sequences could not be detected in plethodontids belonging to different tribes, nor in more distantly related amphibians. The profiles of binding of the common repetitive sequences to CsCl or CS2SO4-Ag+ density gradient fractions of P. dunni DNA suggested that these sequences consisted of heterogeneous components with respect to base compositions, and that they did not include large amounts of the genes for ribosomal RNA, 5S RNA, 4S RNA, or histone messenger RNA. In situ hybridization of the 3H-labelled intermediate repetitive sequences of P. cinereus to male meiotic chromosomes of the same species gave autoradiographs after an exposure of seven days showing all 14 chromosomes labelled. The pattern of labelling appeared not to be random, but was impossible to analyse on account of the irregular shapes and different degrees of stretching of diplotene and prometaphase chromosomes. In situ hybridization of the same sequences to meiotic chromosomes from P. dunni gave autoradiographs after 60 d exposure in which all chromosomes were labelled. These heterologous in situ hybrids can only have involved the "common" repetitive sequences.

Amphibians↗