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Biomedical subjects

S Mizuno

Publications and source records attributed to S Mizuno.

At least 559 records · Page 31Linked to original sources
PubMed1983

A new technique in echocardiology: B-mode to M-mode transformation.

In conventional two-dimensional Echocardiography with sector scanning, M-mode is simultaneously displayed on TV-monitor as the time based ultrasound signals. With conventional system, one end of the cursor-line in B-mode image which indicates the beam direction of M-mode, is always fixed at the point corresponding to the transducer tip. This system has the capability to transform B-mode to M-mode images in any desired direction. However, the time resolution in M-mode is limited to about 33 milli-seconds.

Biometry↗
PubMed1983

[Screening for colorectal carcinoma in AMHTS (automated multiphasic health testing and services)].

A mass survey program for the early detection of colorectal carcinoma was tested on asymptomatic persons who visited the AMHTS (Ningen-Dokku). During a 10-month period, 4574 of 8216 persons over the age of 45 years participated in this program. They were screened by the 3-day fecal occult blood test using guiac method. Barium enema examination was performed in 50 of 84 persons who were selected by the screening tests. Tow cases of advanced carcinoma and 10 adenomas were detected.

Adult↗
PubMed1982

Demonstration of W chromosome-specific repetitive DNA sequences in the domestic fowl, Gallus g. domesticus.

Evidence is presented to demonstrate the presence of W chromosome-specific repetitive DNA sequences in the female White Leghorn chicken, Gallus g. domesticus, based on two different experimental approaches. First, 3H-labelled, female chicken DNA was hybridized with excess, unlabelled, mercurated, male DNA, and unhybridized single-stranded 3H-DNA (3H-SHU-DNA) was recovered by SH-Sepharose and hydroxyapatite column chromatography. Approximately 24% of the hybridizable 3H-SHU-DNA was female-specific and localized on the W chromosome. The second approach was to examine female-specific DNA fragments among the digests of chicken DNA with various restriction endonucleases. Among them, we found that digestion with XhoI produced two prominent female-specific brands of 0.60 kb (= kilobase pairs) and 1.1 kb. The 0.60 kb fragment was isolated and 3H-labelled by nick-translation. Female-specificity of the 3H-XhoI--0.60 kb DNA was judged to be at least 95% under the conditions of hybridization with membrane filter-bound DNA. Presence of amplified XhoI--0.60 kb DNA on the W chromosome seems to be limited to different lines of G.g. domesticus and no such repeat was detected in three species belonging to other genera in the order Galliformes and in three species belonging to other avian orders.

Animals↗
PubMed1982

Super-high-affinity binding site for [3H]diazepam in the presence of Co2+, Ni2+, Cu2+, or Zn2+.

Chloride salts of Li+, Na+, K+, Mg2+, Ca2+, Cr3+, Mn2+, Fe2+, and Fe3+ had no effect on [3H]diazepam binding. Chloride salts of Co2+, Ni2+, Cu2+, and Zn2+ increased [3H]diazepam binding by 34 to 68% in a concentration-dependent fashion. Since these divalent cations potentiated the GABA-enhanced [3H]diazepam binding and the effect of each divalent cation was nearly additive with GABA, these cations probably act at a site different from the GABA recognition site in the benzodiazepine-receptor complex. Scatchard plots of [3H]diazepam binding without an effective divalent cation showed a single class of binding, with a Kd value of 5.3 nM. In the presence of 1 mM Co2+, Ni2+, Cu2+, or Zn2+, two distinct binding sites were evident with apparent Kd values of 1.0 nM and 5.7 nM. The higher-affinity binding was not detected in the absence of an effective divalent cation and is probably a novel, super-high-affinity binding site.

Animals↗
PubMed1982

Relationship between serotype and certain biological and biochemical characteristics of strains of the Mycobacterium avium and Mycobacterium intracellulare complex.

Some relationships among Schaefer's serotypes and biological and biochemical characteristics were observed in strains of the Mycobacterium avium-Mycobacterium intracellulare complex. Strains belonging to serotypes 2 and 16 lacked the capacity to utilize n- and iso-butanols as the sole source of carbon in the presence of ammoniacal nitrogen. However, strains of serotype 2 grew at 45 C and lacked arylsulfatase activity (after 14 days), whereas strains of serotype 16 failed to grow at 45 C and showed positive arylsulfatase activity. Strains belonging to serotypes 8, 9, and 15 grew at 45 C, utilized n- and iso-butanols, and showed arylsulfatase activity.

Arylsulfatases↗
PubMed1982

Effects of hyperthermia and ethanol on the cytotoxicity of bleomycin, adriamycin, cis-diamminedichloroplatinum(II) and macromomycin toward hela cells.

The cytotoxic effect of bleomycin on HeLa S3 cells is greatly enhanced when the cells are exposed to ethanol before drug treatment or treated with the drug at 43 degrees (hyperthermic temperature). On the other hand, the cytotoxic effects of adriamycin, cis-diamminedichloroplatinum(II) and macromomycin are not enhanced by ethanol exposure, but are potentiated by 43 degrees hyperthermia. This effect of ethanol suggests a unique mode of action in sensitizing cells to bleomycin.

Anti-Bacterial Agents↗
PubMed1982

Selective enhancement of the cytotoxicity of the bleomycin derivative, peplomycin, by local anesthetics alone and combined with hyperthermia.

The effect of local anesthetics alone and combined with hyperthermia on the cytotoxic effect of the bleomycin derivative, peplomycin, was studied in FM3A and HeLa cells. Noncytotoxic doses of the local anesthetics procaine, lidocaine, butacaine, tetracaine, and dibucaine enhanced peplomycin cytotoxicity. This enhancement correlated with the reported anesthetic potency of these agents. Combination of lidocaine (3 to 6 mM) and moderate hyperthermia (40 and 41 degrees) greatly enhanced peplomycin cytotoxicity, although these doses of lidocaine alone were ineffective at 37 degrees, and the temperatures alone enhanced the cytotoxicity only slightly. Cell sensitization to peplomycin cytotoxicity induced by lidocaine combined with 41 degrees hyperthermia produced a decrease in cell survival that depended on the dose of lidocaine and the dose and duration of peplomycin treatment. Lidocaine at 37 or 41 degrees did not enhance the cytotoxicity of Adriamycin, mitomycin C, and cis-diamminedichloroplatinum(II), suggesting a unique interaction with peplomycin. The enhancing effect of lidocaine on peplomycin-induced cell killing was found to increase as pH increased within the range of 7.0 to 8.0.

Anesthetics, Local↗