Search PubMed⌕ Search

Biomedical subjects

S Miyazaki

Publications and source records attributed to S Miyazaki.

1,017 records · Page 57Linked to original sources
PubMedDate not supplied

Analysis of c-myc oncogene in human esophageal carcinoma: immunohistochemistry, in situ hybridization and northern and Southern blot studies.

We have examined the c-myc gene expression and the gene organization in resected human esophageal squamous cell carcinomas, and in the adjacent normal esophageal mucosa from 20 patients undergoing radical surgery. Immunohistochemistry of p62c-myc was compared with that of proliferating cell nuclear antigen (PCNA) in order to examine the biologic significance of p62c-myc. Relative c-myc expression detected by Northern blot analysis ranged from 0.41 to 2.8, but the degree of c-myc expression did not correlate with other clinicopathological prognostic parameters. In sity hybridization localized the elevated c-myc mRNA expression to tumor cells and basal and parabasal cells of the adjacent normal mucosa. Immunohistochemistry showed altered localization of p62c-myc, i.e., both cytoplasmic and nuclear immunostaining in advanced carcinomas. c-myc immunoreactivity exhibited wider distribution compared with that of PCNA, a cell cycle related antigen, which may indicate induction of cell proliferation by p62c-myc. DNA hybridization showed mild amplification in one out of 17 tumors and no evidence of gene rearrangement. There was no distinct correlation between the results of Northern or Southern blot analysis and the results of in situ hybridization or immunohistochemistry. Gene alteration of the c-myc locus, as well as overexpression of the c-myc oncogene, appeared to be limited, and analysis of the c-myc gene yielded limited prognostic value in human esophageal carcinomas.

Aged↗
PubMedDate not supplied

[Currently used orthodontic alloys. Review (1)].

A review of currently used alloys in orthodontic is presented in this first part of two articles, The mechanical properties of clinical relevance of various orthodontic wires is described. The alloys considered are Stainless Steel, Cobalt Chromium, Nickel Titanium and Beta Titanium.

Chromium Alloys↗
PubMedDate not supplied

Expression of c-erbB-2 in human esophageal carcinoma cells: overexpression correlated with gene amplification or with GATA-3 transcription factor expression.

Amplification and expression of the c-erbB-2 protooncogene was studied in 60 primary human esophageal carcinomas and 12 cell lines established from the carcinomas. Amplification of the c-erbB-2 gene was found in 4 out of 60 (6.7%) and 1 out of 12 (8.3%) DNA samples from the primary carcinomas and cell lines, respectively. Overexpression of c-erbB-2 gene product, as determined by both Northern blot hybridization and immunohistochemical analysis, was found in the cells with c-erbB-2 amplification, but not in normal esophageal mucosae or in primary esophageal carcinomas without gene amplification. In contrast, c-erbB-2 overexpression was clearly observed in 7 cell lines in which gene amplification was not found. We analyzed expression of the GATA-3 transcription factor in the carcinoma cell lines and found that the level of GATA-3 mRNA expression showed very good correlation with that of c-erbB-2 gene expression. This observation suggested that the GATA-3 transcription factor and c-erbB-2 protein play important roles in the process of immortalization and/or maintenance of the transformed phenotype of human esophageal carcinoma cells in vitro.

Adenocarcinoma↗
PubMedDate not supplied

BK virus-induced osteosarcoma (Os515) as a model of human osteosarcoma.

BK virus was isolated by Gardner et al in 1971 from the urine of an immunosuppressed patient with a kidney allograft. Antibodies to this virus are ubiquitous in the general populations worldwide, but the oncogenic capacity of BKV in humans had not been reported. The virus transformed in vitro permissive human and non-permissive animal cells, and the transformed cells had the T antigen. Intracerebral and intravenous inoculation of BKV in newborn hamsters induced malignant tumours (mainly ependymomas, malignant insulinomas, and osteosarcomas). Subcutaneous and intraperitoneal routes were also effective. The virus was only rescued from a few tumours by fusion with human embryonic cells or Vero cells. Brain tumours appeared earlier and osteosarcomas developed in animals which survived for more than 6 months. Many of the osteosarcomas were bony and grew slowly with frequent lung metastases, and a few osteosarcomas were soft and grew rapidly without lung metastases. Experimental targeting chemotherapy with doxorubicin (DX)-containing immunoliposomes was performed against Os515 osteosarcoma. In in vitro experiments, DX-Lip-MoAb29 showed a more significant inhibitory effect on cultured Os515 cells than free Dx and DX-Lip. DX-Lip DNP had less effect. In in vivo experiments, DX-Lip-MoAb29 suppressed the growth of Os515 tumour isografts in hamsters and prolonged the survival of recipients more significantly than free DX.

Animals↗
PubMedDate not supplied

Effects of betahistine, a histamine H1 agonist and H3 antagonist, in a light/dark test in mice.

The effects of betahistine, a histamine H1 agonist and H3 antagonist, were investigated in a light/dark test measuring anxiety in mice. Betahistine significantly decreased the locomotion and rearing in the light and dark zones, shuttle crossing and time spent in the light zone in the light/dark test. These phenomena suggest that betahistine has anxiogenic effects and/or locomotor suppressive effects. Pyrilamine (6 mg/kg) and ketotifen (6 mg/kg), H1-receptor antagonists, antagonized the effects of betahistine (100 mg/kg) on all parameters in the light/dark test but zolantidine (6 mg/kg), an H2-receptor antagonist, augmented its effects on some parameters decreased by betahistine. Especially, it potentiated the decreases by betahistine in the parameters showing anxiety in this test. The decreases in the light/dark test induced by zolantidine (6 mg/kg) plus betahistine (200 mg/kg) were antagonized by pyrilamine (12 mg/kg) but antagonism of the decrease in the time spent in the light zone as a parameter showing anxiety was not significant. These results suggest that the effects via H1 receptors are involved in anxiety in the light/dark test in mice but the other factor may be simultaneously needed for induction of behaviorally detectable anxiogenic effects in this test.

Animals↗
PubMedDate not supplied

Injection sclerotherapy for gastric varices using N-butyl-2-cyanoacrylate and ethanolamine oleate.

BACKGROUND/AIMS: Tissue adhesive agents, such as the cyanoacrylates, have been used as an alternative to conventional sclerotherapy to treat gastric varices, but the long-term efficacy of this approach has not been determined. We evaluated the efficacy and long-term outcome of injection sclerotherapy with n-butyl-2-cyanoacrylate and ethanolamine oleate in 16 patients with gastric varices. METHODOLOGY: We evaluated the effect of injection sclerotherapy in 16 Japanese patients with gastric varices. Injection sclerotherapy was performed on an emergency basis in 6 patients, an elective basis in 5 patients, and as prophylaxis in 5 patients. RESULTS: No bleeding was observed in the 7 patients in whom gastric varices disappeared during the 51 month follow-up period. The non-bleeding rate after treatment was significantly higher in this group than in the 9 patients in whom gastric varices did not disappear (p<0.05). Acute bleeding was stopped in 5 (83.3%) of 6 patients. The single failure was a patient in whom the sclerosant could not be injected into the gastric varices. No serious complications, such as emboli in other organs, were observed. CONCLUSION: The results suggest that this therapy is a safe and useful treatment for gastric varices and that the goal of injection sclerotherapy should be the disappearance of gastric varices.

Disease-Free Survival↗