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Biomedical subjects

S Miyata

Publications and source records attributed to S Miyata.

At least 253 records · Page 14Linked to original sources
PubMed1980

Determination of manganese in tissues by neutron activation analysis using an antimony pentoxide column.

A rapid and accurate method is presented for the determination of manganese in biological samples, using neutron activation analysis. Biological samples were irradiated at 5000 kW for 30 min. The samples were ashed on a hot plate with 14 mol/l HNO3 and 6 mol/l HClO4, and resolved in 1 mol/l HClO4. 24Na and 24K were removed by passing each sample through in antimony pentoxide column. 54Mn was added as a tracer to calculate the ratio of manganese recovered by the separation procedure. Recovery was over 90%. This method was applied in order to determine manganese in various tissues. In the cervical spinal cord of the controls, the mean manganese concentrations in the anterior horn, the lateral and the posterior columns were 1.14, 1.06 and 0.90 ng/mg of dried tissue, respectively. In two cases if amyotrophic lateral sclerosis the manganese concentrations in the cervical spinal cord were elevated, particularly in the anterior horn and the lateral column.

Amyotrophic Lateral Sclerosis↗
PubMed1980

A bradykinin-like substance in rat stomach.

A bradykinin (BK)-like substance (P1) in the rat stomach was extracted with acetic acid, n-butanol, distilled water and methanol. The gradient and equilibrium chromatography were carried out on SP-Sephadex C-25 columns with the extract containing P1. P1 had a different retention time from BK, kallidin and methionyl-lysyl-bradykinin (MLBK), on the equilibrium chromatography. The apparent molecular weight of P1 estimated by gel chromatography was over 1,300. P1 was classified as a biologically BK-like peptide of mammalian origin which is distinct from BK, kallidin and MLBK. Another kind of biologically active substance (P2) which contracts the isolated rat uterus and duodenum was detected during the course of the extraction and purification of P1. The contractile activity of P2 was abolished by the presence of dibenamine or methysergide, but was not influenced by chymotrypsin, trypsin or papain digestion. The hypotensive effect of P2 on rabbit blood pressure was similar to that of serotonin (5-HT). The retention times of P2 on the equilibrium chromatography on the SP-Sephadex C-25 column, and on the gel chromatography were the same as those of 5-HT. P2 proved to be 5-HT.

Animals↗
PubMed1980

[Two dimensional analysis of floor reaction force in normal gait (author's transl)].

In order to estimate the gait with force plate, two dimensional analysis with Lisajou's figure composed of two of the three components of floor reaction force was attempted. In normal gait, sagittal component (alpha-diagram) appears as an inverted heart pattern, frontal component (beta-diagram) as a S-shaped pattern and horizontal component (gamma-diagram) as a Gourd-shaped pattern. The most important external force which acts on the body during progression is the sagittal component of floor reaction force, so that the author used the alpha-diagram which was composed of vertical and fore and aft forces for the purpose of gait analysis in normal adults. This study was conducted on 100 normal adults, 50 males and 50 females ranging in age from eighteen to thirty-five years. alpha-diagram was classified into 6 types in males and 5 types in females based on the shape of alpha-diagram and discriminant functions and there were some difference depending on the individual, sex and side was noted. Male differed from female mostly during the heel strike phase. Propulsion forces of dominant side was smaller than that of the other side.

Adolescent↗
PubMed1976

Glucosamine metabolism in regenerating rat liver.

1. Glycoprotein synthesis was investigated with [1-14C]glucosamine in vivo. [14C]Glucosamine was administered intravenously 24h after hepatectomy to rats. 2. Incorporation into the acid-soluble fraction was maximum at 15 min after injection both in sham-operated and hepatectomized rats. 3. Enhancement of incorporation into UDP-N-acetylhexosamine in regenerating liver was observed. However, its specific activity was lower, because of a greater enhancement of synthesis de novo of the amino sugar. 4. In the liver acid-insoluble fraction, maximum incorporation of [14C]glucosamine was at 30 min in sham-operated rats and 2 h in hepatectomized rats respectively. 5. In sham-operated rats, incorporation into the plasma acid-insoluble fraction followed that of the liver acid-insoluble fraction, but hepatectomy resulted in a rapid enchancement of incorporation into plasma. 6. It is concluded that synthesis of liver glycoproteins is stimulated after partial hepatectomy and that glycoproteins synthesized are released rapidly into the plasma.

Animals↗