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Biomedical subjects

S Miyachi

Publications and source records attributed to S Miyachi.

119 records · Page 7Linked to original sources
PubMed1966

Sulfolipid metabolism in chlorella.

When S-deficient cells of Chlorella cllipsoidea were incubated in radio-sulfate in light or in aerobic darkness for 1 hour, equal amounts of radioactivity were found in sulfolipid and glutathione but none was detected in sulfoquinovosyl glycerol which is one of the major S-compounds in this alga. No assimilation of radiosulfate was observed under anaerobic darkness.To elucidate the function of sulfolipid in algal cells uniformly (35)S-labeled Chlorella cells were transferred to S-deficient culture medium or unlabeled normal culture medium and the changes of radioactivity in sulfolipid and the related compounds were followed. A) On incubating (35)S-labeled algal cells in S-deficient medium under photosynthetic conditions, the amounts of radioactivity in sulfate, sulfoquinovosyl glycerol and sulfolipid decreased rapidly. B) When (35)S-labeled cells were cultured photoautotrophically in unlabeled medium, no decrease of radioactivity was observed in sulfoquinovosyl glycerol and sulfolipid. C) A decrease of (35)S-sulfolipid and an increase of (35)S-sulfoquinovosyl glycerol were observed when the uniformly (35)S-labeled algal cells were illuminated in CO(2)-free air.When S-deficient Chlorella cells were incubated in (35)S-sulfolipid under photosynthetic conditions, significant radioactivity was found in the insoluble fraction of the cells. A similar result was observed when normal Chlorella cells were incubated in (14)C-sulfolipid and CO(2)-free air.It is inferred from these observations that sulfolipid is a reservoir of sulfur and carbon compounds.In order to ascertain if the sulfolipid is involved in the mechanism of photosynthetic oxygen evolution, the rate of photosynthesis was measured during the incubation of (35)S-labeled cells in a S-deficient medium. Parallelism was not observed between the rate of photosynthetic activity and the decrease of sulfolipid.

Journal Article↗
PubMedDate not supplied

The effect of sodium hyaluronate on the expression of gelatinases in rabbit corneal epithelial wound healing.

PURPOSE: We investigated the effect of sodium hyaluronate (Na-HA) on the expression of gelatinases in a rabbit model with corneal epithelial defects. METHODS: Topical administration of Na-HA or phosphate-buffered saline (PBS) was carried out in the experimental eyes after surgical removal of the corneal epithelium. At 0, 6, 24, 48, and 72 hours after wounding, mRNA expression of 72 kDa type gelatinase (MMP-2), 92 kDa type gelatinase (MMP-9), and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) were analyzed by reverse transcription polymerase chain reaction in those corneas. In addition, gelatinolytic activities were investigated using gelatin zymography. RESULTS: The levels of constitutive expression of MMP-2 and TIMP-1 mRNA persisted, whereas MMP-9 mRNA in the PBS-treated side was expressed temporarily after surgical removal. In the Na-HA-treated side, at 6 hours after wounding, a much higher expression of MMP-9 mRNA was reproducibly observed compared with that in the PBS-treated side. In zymography, the levels of gelatinolytic activity corresponding to proMMP-9 were significantly higher in the Na-HA-treated side than in the PBS-treated side at 6 hours after wounding. CONCLUSIONS: These results suggest a novel participation of Na-HA in the expression of MMP-9 in rabbit corneal epithelial wound healing.

Adjuvants, Immunologic↗