For radioimmunoassay of the catechol estrogens, four hapten-bovine serum albumin (BSA) conjugates were prepared from 6-oxo-2-hydroxyestradiol 6-(O-carboxymethyl)oxime, 2-hydroxyestradiol 17-hemisuccinate, 6-oxo-4-hydroxyestradiol 6-(O-carboxymethyl)oxime and 4-hydroxyestradiol 17-hemisuccinate by coupling with BSA, employing the mixed anhydride method. The antisera elicited in rabbits by immunization with these antigens showed high affinity and specificity for 2-hydroxyestradiol or 4-hydroxyestradiol with cross-reactivities to a few structurally related estrogens. The specificity of antisera obtained is discussed in relation to the site of attachment of the hapten to BSA.

Animals↗

PubMedDate not supplied

The effect of neutrophil depletion on reperfusion arrhythmias during intracoronary thrombolysis using the leukocyte removal filter.

To evaluate the effects of neutrophil depletion on reperfusion arrhythmias using a leukocyte removal filter (LRF), the authors examined several cardiovascular variables in three patients with myocardial infarction who underwent successful thrombolytic therapy with LRF compared with control patients without LRF. Coronary angiography performed within 4 hr of onset revealed total occlusion in the proximal segment of the left anterior descending (LAD) coronary artery, without collaterals, in all patients. Thrombolysis was performed by intracoronary administration of urokinase (10,000 IU/ml, 1,000,000 IU total) mixed with filtered autologous blood. LRF was composed of a nonwoven polyester fabric (1.8 microns, 4.6 g). Removal rates of leukocytes and platelets by LRF were 98-100% and 98%, respectively. Average duration of the ischemic episodes, maximum ST segment elevation, and integral of T segment depression did not differ in the two groups. However, the frequency of ventricular premature beats (VPB) during the first 30 min after reperfusion were significantly lower in the LRF treatment group (3.8%) compared with the control group (10.7%). Segmental shortening, measured by echocardiography one hour after recanalization, exhibited higher values in the LRF treated patients than control patients, without significant difference. Data suggested that LRF is effective in reducing reperfusion arrhythmias during intracoronary thrombolysis and activated neutrophils may play an important role in stunned myocardium.

Adult↗

PubMedDate not supplied

Reappraisal of OP-1206, a prostaglandin E1 derivative in combination with low-flow oxygen inhalation therapy in chronic lung disease.

For the purpose of alleviating pulmonary hypertension and maintaining cardiac output, which tended to be decreased to acute low-flow oxygen therapy in chronic lung diseases, we evaluated combined low-flow oxygen therapy and oral administration of OP-1206, a prostaglandin E1 derivative, in 7 patients with obstructive lung disease and 3 with restrictive one. Low flow oxygen inhalation (1 l/min. 30 minutes) increased PaO2 from 67.2 +/- 12.8 mmHg to 91.0 +/- 18.2 mmHg (p less than 0.001), PVO2 from 36.4 +/- 3.1 mmHg to 41.7 +/- 7.0 mmHg (p less than 0.01) and decreased cardiac index from 3.38 +/- 0.39 l/min/m2 to 3.07 +/- 0.32 l/min/m2 (p less than 0.01), RVSWI from 18.7 +/- 2.6 g.m/m2 to 6.1 +/- 2.0 g.m/m2 (p less than 0.01), transpulmonary driving pressure from 13.0 +/- 5.4 mmHg to 9.8 +/- 5.5 mmHg (p less than 0.01) and mean PA from 21.6 +/- 7.7 mmHg to 18.3 +/- 6.9 mmHg (p less than 0.05) with a trend of increase in COD and no change at oxygen delivery. OP-1206, on the contrary, decreased mPA from 21.6 +/- 7.7 to 18.2 +/- 5.6 mmHg (p less than 0.05), RVSWI from 8.7 +/- 2.6 g.m/m2 to 6.9 +/- 2.3 g.m/m2 (p less than 0.01) with trend of decrease in transpulmonary driving pressure, TPVR and PAR with no substantial changes in gasometric parameters. The decrease rates of PA by low-flow oxygen therapy and OP-1206 administration correlated significantly.(ABSTRACT TRUNCATED AT 250 WORDS)

Administration, Oral↗

PubMedDate not supplied

Inappropriate secretion of adrenocorticotropin from corticotroph hyperplasia in a case of Addison's disease.

A patient with Addison's disease, treated with conventional hydrocortisone replacement, developed deep hyperpigmentation, headache and vomiting. Plasma adrenocorticotropin (ACTH) level was extremely high, showing abnormal diurnal rhythm. Suppression of ACTH with glucocorticoids was attenuated and the responses to ovine corticotropin-releasing hormone (oCRF) and lysine vasopressin (LVP) were absent. Magnetic resonance imaging (MRI) suggested an enlargement of the pituitary gland, while immunohistological examination of pituitary fragments obtained by transsphenoidal surgery revealed corticotroph hyperplasia without microadenoma. Postoperatively, plasma ACTH returned to normal and adequately responded to oCRF and LVP. Over the year since surgery, the symptoms have gradually improved and the patient has resumed normal activities.

Addison Disease↗