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Biomedical subjects

S Misra

Publications and source records attributed to S Misra.

At least 181 records · Page 10Linked to original sources
PubMed1985

A comparative study of human placental and fetal liver catalase during development.

The activity and a few properties of catalase have been compared in the developing human placenta and fetal liver. The presence of the enzyme in both the tissues is discernible as early as in the 6th wk of gestation and the activity increases gradually with the advancement of pregnancy. Maximum enzyme activity in both placenta and fetal liver is found to be associated with the soluble supernatant fraction obtained by centrifuging the tissue homogenates at 105000 X g. Kinetic studies reveal the enzymatic decomposition of H2O2 to follow first-order kinetics at lower substrate concentrations, and then to deviate from the original linearity, demonstrating mixed-order kinetics. Thermostability of placental catalase increases with prenatal development, while the enzyme from fetal liver remains moderately heat-stable throughout the gestation. Treatment of the homogenates with Triton X-100 is found to be most effective in increasing catalase activity in each of these tissues.

Catalase↗
PubMed1985

Glutamine metabolism in corn kernels cultured in vitro.

The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvested at intervals up to 168 hours. Protein and starch fractions were then separated and analyzed for their (14)C content. At 168 hours, 31% of the total label incorporated in the endosperm was in zein, 15% in glutelin, and 24% in starch. When individual amino acids and sugars in the endosperm powders were analyzed, the (14)C still remaining in the glutamine accounted for only 12 to 14% of the total radioactivity.

Journal Article↗
PubMed1985

Reprogramming of Protein Synthesis from a Developmental to a Germinative Mode Induced by Desiccation of the Axes of Phaseolus vulgaris.

Immature seeds of Phaseolus vulgaris cv Taylor's Horticultural removed from the pod at 32 days of development do not germinate unless first subjected to desiccation. Our results show that premature drying not only redirects metabolism from a developmental to a germination program but it does so permanently, thus effecting an irreversible switch. This is shown by in vitro protein synthesis, and analysis of poly(A)(+) mRNA with a cDNA probe specific for phaseolin message. For example, the pattern of proteins synthesized in vitro by the mRNA fraction from fresh and prematurely dried axes show strong similarities; on the other hand, the mRNA population from rehydrated axes code for a different set of proteins. Also, the message for phaseolin is preserved following the normal maturation process and premature desiccation of seeds. Following rehydration of immature seeds at the desiccation-tolerant stage, this message is no longer detectable in the axes.

Journal Article↗
PubMed1985

Allogeneic leukocyte and germ cell-induced murine immunodeficiency.

Immune deficiency, as defined by significant decreases in lymphocyte Con A and allo-reactivity and in natural killer (NK) function, was induced in normal adult mice by i.p. injections of combinations of allogeneic testicular germ cells and splenic leukocytes over 3 wk. This immune deficiency was evident at 8 wk after initial injection, and profound by 12 wk. Neither leukocytes nor testicular cells, given alone, were able to induce similar immune deficiency. These findings suggest the possibility that allogeneic germ cells and leukocytes of semen, on repeated administration, may induce immune deficiency and may act as co-factors to viral agents in the development of clinical AIDS in humans.

Animals↗
PubMed1985

Studies on the causation of adverse reactions in microfilaraemic host following diethylcarbamazine therapy (Dipetalonema viteae in Mastomys natalensis).

The mechanism of the adverse reaction in microfilaraemic subjects following diethylcarbamazine (DEC) therapy has remained an enigma. To study the mechanisms involved Mastomys natalensis infected with Dipetalonema viteae have been used. Animals showing up to 50 mf/5 cmm of blood did not show any perceptible reactions when treated with the minimum effective dose of 350 mg/kg of DEC citrate, while those having more than 80 mf/5 cmm died, suggesting an intensity dependent severity of reactions. Infective larvae contained maximum amounts of biogenic amines followed by mf and adults in that order. 16 mg of histamine or 24 mg of 5-hydroxytryptamine caused death when injected into healthy animals, whereas sonicated mf (5 million) and adult somatic products (4 mg protein) did not, signifying that the biogenic amine contents of the parasites were less than the critical level to cause death. Adult somatic products did not produce any visible reactions when injected into animals with microfilariaemia, but sonicated mf (5 million) caused death. It is concluded that the adverse reactions seen after DEC treatment are due to the release of biogenic amines in an anaphylactic type of reaction subsequent to massive release of parasite antigen in a sensitised host, which is directly proportional to the intensity of microfilaraemia and that the adverse reactions are not due to liberation of endogenous biogenic amines from dead mf.

Animals↗
PubMed1983

Adult polio-like syndrome following Enterovirus 70 conjunctivitis (natural history of the disease).

During the Indian EV 70 conjunctivitis epidemic in 1981, 79 patients with neurological complications were evaluated by clinical, electrophysiological, histopathological and virological studies. The disease was heralded by an attack of conjunctivitis and was followed, after a variable latent period, by a pre-paralytic, paralytic, and post-paralytic stage of slow recovery. The above events occurred in an orderly sequence. The paralysis affected the cranial nerves, limbs or both. The clinical course closely simulated poliomyelitis, without any evidence of radiculopathy or myelopathy. Cranial nerve palsies were seen in approximately half of the patients. The electrophysiological and histologicl studies pointed towards the affection of anterior horn cells. The raised neutralizing antibody titres to Enterovirus 70, confirmed the aetiology of the disease. On the available data, we believe that this nosological entity should be considered under the general title of "polio-like syndrome" following EV 70 conjunctivitis. On account of two epidemics in 1971 and 1981 with unfavourable prognosis and high resultant disability, and because of lack of specific therapy, it has become imperative to develop, as a top priority, a vaccine for immunization.

Adult↗
PubMed1983

Meatoplasty.

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Humans↗
PubMed1982

Psychological factors and recovery from cardiac surgery.

21 Subjects awaiting open-heart surgery were given personality and attitude questionnaires. They were re-tested six months after the operation, when they were divided into "completely recovered" (I) and "not completely recovered" (II) groups, according to the New York Heart Association classification. Results showed that Group I was significantly less depressed, more optimistic about the future and had a more positive attitude towards others. Group II realised they had been very ill and had a more negative attitude towards their spouses. Group I was found to be much better adjusted, vocationally and psychosexually, than Group II.

Adaptation, Psychological↗
PubMed1982

Age-at-onset of leprosy. (An analytic data from Northern India).

Very little information is available from Northern India about various intriguing facets of epidemiology of leprosy. Age-at-onset is a very important aspect of the epidemiology of the disease process. In the present study an analytic data of the patients from Northern India is presented. The mean age-at-onset of leprosy in males was 31.49 years and in female 29.43 years. The mean age-at-onset for N, N ? L and L types was 30.14, 30.12 and 34.13 years respectively. The data compared well with those of earlier series.

Adult↗