Search PubMed⌕ Search

Biomedical subjects

S Mintz

Publications and source records attributed to S Mintz.

65 records · Page 4Linked to original sources
PubMed1971

Redox state of free nicotinamide-adenine nucleotides in the cytoplasm and mitochondria of alveolar macrophages.

Cytoplasmic free NAD(+)/NADH ratios have been calculated from lactate to pyruvate ratios, and mitochondrial NAD(+)/NADH ratios, have been calculated from beta-hydroxybutyrate to acetoacetate ratios in isolated rabbit alveolar macrophages. In freshly harvested cells, assuming a pH of 7 for the two compartments, cytoplasmic NAD(+)/NADH averaged 709 +/-293 (SD), and mitochondrial NAD(+)/NADH averaged 33.2 +/-30.2, values which are significantly different. 30 min of air incubation in a relatively poorly buffered medium showed a significant reduction in calculated mitochondrial NAD(+)/NADH to 10.1 +/-4.8. 30 min of exposure of cells to a hypoxic environment (equivalent to a nonventilated, perfused alveolus) caused significant reductions of NAD(+)/NADH in both compartments. Re-exposure of hypoxic cells to air produced a change toward normal in cytoplasmic NAD(+)/NADH but did not reverse mitochondrial abnormality. Uncertainties concerning the value of cytoplasmic and mitochondrial pH under control conditions and during experimental pertubations, limit absolute interpretation of NAD(+)/NADH ratios calculated from redox pairs, but the data suggest the following: (a) separate cytoplasmic and mitochondrial compartments for NAD(+) and NADH exist in the alveolar macrophage; (b) brief periods of exposure to moderate hypoxia of the degree seen in clinical lung disease produce decreases in both cytoplasmic and mitochondrial NAD(+)/NADH; (c) the mitochondrial changes are less easily reversed than the cytoplasmic changes; (d) measurements of NAD(+)/NADH provide an early sensitive indication of biochemical abnormality; and (e) careful control of extracellular pH is required in studies involving experimental modifications of alveolar macrophage function.

Animals↗
PubMedDate not supplied

Gorham's disease affecting the maxillofacial skeleton.

Twenty-one cases of Gorham's disease in the maxillofacial region are described in the literature. This case is an additional one. The disease has a slight male predominance. All cases involved either the mandible alone or in association with the maxilla or other bones of the head and neck. The most common symptoms were pain, tooth mobility, mandibular pathological fractures, and facial deformity. The mean duration of symptoms prior to diagnosis was 6.4 years. Treatment included surgery, radiotherapy, and various medications, alone or combined. Four patients died of their disease. The diagnosis of the initial biopsy was incorrect in 45% of these cases. The current case appears to be the only one successfully treated by excision and autogenous bone graft reconstruction. In order to make an accurate diagnosis, the histologic features should be correlated with the clinical features.

Adolescent↗