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Biomedical subjects

S Minami

Publications and source records attributed to S Minami.

At least 379 records · Page 21Linked to original sources
PubMed1980

Purification and properties of a cephalosporinase from Enterobacter cloacae.

A cephalosporin beta-lactamase (cephalosporinase) was extracted from Enterobacter cloacae GN7471 and purified by means of column chromatography. The resulting preparation gave a single protein band upon polyacrylamide gel electrophoresis. The enzyme's isoelectric point was 8.4, and its molecular weight was 44,000. The optimal pH was 8.5, and the optimal temperature was 40 degrees C. The enzyme hydrolyzed cephalosporins much more readily than penicillins. The enzyme activity was inhibited by iodine, semisynthetic penicillins, cefuroxime-type cephalosporins, and cephamycin derivatives. The enzymological properties of the purified enzyme were compared with those of beta-lactamases derived from other gram-negative enteric bacteria.

Cephalosporinase↗
PubMed1980

Bioconversion and biosynthesis of nanaomycins using cerulenin, a specific inhibitor of fatty acid and polyketide biosyntheses.

The biosynthetic relationship of the nanaomycins produced by Streptomyces rosa var. notoensis OS-3966 was studied by means of a bioconversion method using the antibiotic cerulenin, a specific inhibitor of fatty acid and polyketide biosyntheses. Nanaomycin D was considered to be the first component produced from the hypothetical intermediate "polyketide". It is proposed that the biosynthesis sequence for the nanaomycin is: nanaomycin D leads to nanaomycin A leads to nanaomycin E leads to nanaomycin B. Nanaomycin B can be converted to nanaomycin A by non-enzymatic dehydration; however, nanaomycin A is rapidly bioconverted to nanaomycin E, which is the major component synthesized by the nanaomycin-producing strain.

Antifungal Agents↗
PubMed1980

[Alveolar septal changes in idiopathic pulmonary fibrosis (author's transl)].

To clarify the mechanism of the disturbance of alveolar gas exchange in idiopathic pulmonary fibrosis, various pulmonary function tests were performed on 7 patients with the disease, of which 5 were defined histo-pathologically and 2 clinically. On 4 of all patients, detailed comparative studies of histopathological findings to pulmonary functions were carried out additionally. As the results, marked decreases of total lung capacity, pulmonary diffusing capacity (DLco) and Pao2 were observed. On exercise, the improvement of DLco or physiologic dead space (VD/VT) was hardly seen, while AaDO2 increased markedly. Ventilation (VA), perfusion (Q), and VA/Q ratio examined by 133Xe scintigram distributed more evenly over the entire lung fields than controls. These may suggest that the disturbance of alveolar gas exchange is mainly due to the elongation of the distance for diffusion in the membrane.

Aged↗
PubMed1979

In vitro antibacterial activity of cefoperazone (T-1551), a new semisynthetic cephalosporin.

Cefoperazone, a new semisynthetic cephalosporin, has a broad spectrum of antibacterial activity. It is as active as cefazolin and cefamandole against gram-positive bacteria and is more active than cefazolin and cefamandole against such gram-negative bacilli as Escherichia coli, Klebsiella pneumoniae, Proteus species, Pseudomonas aeruginosa, Citrobacter freundii, Enterobacter cloacae, and Serratia marcescens. The superiority of cefoperazone over cefazolin and cefamandole with respect to activity against P. aeruginosa by more than 200-fold was especially remarkable. As with other beta-lactam antibiotics, there was only a small spread between the minimum inhibitory concentrations and the minimum bactericidal concentrations of cefoperazone and a significant decrease in activity with an increase in inoculum size. Activity was not altered significantly by the addition of human serum to the test medium. Cefoperazone is relatively stable to hydrolysis to beta-lactamases produced by gram-negative bacteria. Relative rates of hydrolysis of cefoperazone by cephalosporinases are 7.0 to 0.01, with reference to cephaloridine hydrolysis (base, 100). Cefoperazone is also more stable than penicillin G and cephaloridine to various types of penicillinases.

Bacteria↗