Biomedical subjects

S Minami

Publications and source records attributed to S Minami.

At least 199 records · Page 11Linked to original sources

PubMed1994

[Secondary leukemia after etoposide treatment involved MLL gene rearrangement].

The 11q23 chromosomal abnormality is frequently observed in infantile leukemia and secondary leukemia, and the translocation associated gene in infantile leukemia is called mixed-lineage leukemia (MLL) gene. A 50-year-old man was admitted because of left axillary lymphadenopathy and IBL like T cell lymphoma was diagnosed by lymph node biopsy. The patient responded to the LSG-9 protocol with complete remission. After 10 months he was readmitted because of fever and was diagnosed acute myeloblastic leukemia by bone marrow aspiration. Chromosome analysis revealed 11q23 abnormality, suggesting that the leukemia was induced by etoposide treatment. Southern blot analysis demonstrated DNA rearrangement in the MLL gene at 11q23. It was suggested that the breakpoint region of the MLL gene in secondary leukemia is the same as that of infantile leukemia.

Chromosomes, Human, Pair 11↗

PubMed1994

[Allogeneic cryopreserved marrow transplantation in a patient with chronic myelogenous leukemia].

A 45-year-old male with chronic myelogenous leukemia received cryopreserved allogeneic bone marrow from his HLA-identical sister. Bone marrow was harvested and cryopreserved prior to chemoradiotherapy since the donor had neurotic tendencies. The preconditioning regimen consisted of standard dosage of busulfan plus cyclophosphamide and total lymphoid irradiation (5Gy). A total of 3.1 x 10(7)/kg marrow mononuclear cells, containing 4.7 x 10(5) CD34+ cells/kg, and 8.0 x 10(6)/kg buffy coat cells collected from the donor at day 0 was infused. Marrow engraftment occurred by day 38 although hematological recovery was delayed and subsequent administration of GM-CSF, methylprednisolone and donor buffy coat cells were required. Mononuclear cells obtained from the patient's blood at day 28 had an inhibitory effect on CFU-GM formation of the donor's bone marrow mononuclear cells. We considered that this case suffered from a transient myelosuppression due to residual host cells after bone marrow transplantation.

Bone Marrow↗

PubMed1994

[Antibacterial activity of tosufloxacin against fresh clinical isolates].

The antibacterial activity of tosufloxacin (TFLX) was compared with those of other new quinolones (ofloxacin, ciprofloxacin, sparfloxacin and fleroxacin) against clinical isolates. These isolates had been collected from May to June 1993 (22 species, 260 strains) and from June to July 1992 (Staphylococcus aureus and Pseudomonas aeruginosa, 87 strains) in Toyama municipal hospital. TFLX showed the most potent activities against methicillin-susceptible S. aureus, methicillin-susceptible Staphylococcus epidermidis, methicillin-resistant S. epidermidis, Enterococcus faecalis and Escherichia coli in 1993, at MIC50 levels. The MIC50's of TFLX for these species ranged from 0.025 to 0.39 microgram/ml. TFLX showed potent activities against Klebsiella pneumoniae and P. aeruginosa, and the MIC50's of TFLX were 0.05 and 0.39 microgram/ml, respectively. However, new quinolone resistant strains (MIC > or = 6.25 micrograms/ml) were found among some species. Especially, the number of methicillin-resistant S. aureus (MRSA) strains resistant to new quinolone exceeded 60% of all the S. aureus isolates in 1993. More MRSA strains resistant to new quinolones were isolated in 1993 than in 1992, and similar result was observed for P. aeruginosa. The coagulase typing was done, and all MRSA strains of resistant to new quinolones produced coagulase type II.

Anti-Infective Agents↗

PubMed1993

Scoliosis associated with syringomyelia.

From January 1986 to December 1991, 1,059 patients with scoliosis of 20 degrees or more were reviewed. By magnetic resonance imaging, 43 patients were found to have syringomyelia. Thirty-eight patients had Chiari type 1 malformation. Twenty-two of the patients were younger than 10 years. Scoliosis was the only presenting symptom in 32 patients. All patients had some neurologic abnormalities. Fifteen patients had left thoracic curves. The incidence of scoliosis with syringomyelia in our outpatient clinic was 4.0%; 18.4% in boys, and 2.6% in girls. Magnetic resonance imaging should be performed in patients who have abnormal neurologic findings.

Adolescent↗

PubMed1993

Developmental expression of the growth hormone receptor gene in the rat hypothalamus.

The ontogeny of growth hormone receptor (GHR) gene expression was studied in the rat hypothalamus. Total RNA from the hypothalamus of rats at different developmental stages (embryonic day 15-56 days of age) was characterized using a 32P-labeled RNA probe derived from the extracellular domain of the rat GHR cDNA. Two RNA species, 4.5 kilobases (kb) encoding for GHR and 1.2 kb encoding for GH-binding protein, were detected in hypothalamic tissue from embryonic day 15 to 56 days of age. Their levels were low at embryonic day 15 and increased toward 3 days of age. The level of 4.5-kb transcript preferentially increased from 7 days after birth, and it was maintained until 35 days of age. Thereafter, the level of 4.5-kb transcript declined. The ratio between the 4.5- and 1.2-kb transcripts was less than 2.0 from embryonic day 15 to 3 days after birth, while it was larger than 4 after 7 days of age. There was no sex difference in the levels or the ratios of the transcripts of the GHR gene from 7 to 56 days of age. The findings indicate that the 4.5-kb transcript preferentially processed postnatally in the rat hypothalamus.

Animals↗

PubMed1993

Barrel rotation evoked by intracerebroventricular injection of somatostatin and arginine-vasopressin is accompanied by the induction of c-fos gene expression in the granular cells of rat cerebellum.

Intracerebroventricular (i.c.v.) injection of somatostatin (SS) or arginine-vasopressin (AVP) elicits barrel rotation (BR) in rats. To identify the potential neuron structures involved in this characteristic behavior, the regional expression of the c-fos gene in rat brain after i.c.v. injection of SS (10 micrograms) or AVP (1 micrograms) was examined by hybridization histochemistry. The c-fos expression could serve as a marker of neuronal activity and/or neural transmission. Following SS-induced BR, c-fos gene expression was observed in the lingula, uvula, nodulus, simplex, centralis, and culmen of the cerebellum, while following AVP-induced BR, c-fos gene expression was observed in the first four of the above-mentioned regions of the cerebellum, but not in the centralis or culmen. In these regions, the c-fos mRNA signals were observed on the granular layer. Expression of the c-fos gene was immediately and transiently induced and was not observed in rats in which BR was not evoked after SS or AVP injection. In both control rats and SS- or AVP-injected rats, the c-fos gene expression was induced in the piriform cortex and the flocculus of the cerebellum. The findings suggest that BR is a manifestation of behavior induced by massive transsynaptic activation of the granular cells in the cerebellum.

Animals↗

PubMed1993

Acute renal failure and degenerative tubular lesions associated with in situ formation of adenovirus immune complexes in a patient with allogeneic bone marrow transplantation.

We describe the development of acute renal failure and degenerative tubular lesions associated with local immune deposits in a patient with allogeneic bone marrow transplantation. A 21-year-old man with an acute myelocytic leukemia received a bone marrow graft from a cousin mismatched for a single HLA-DR locus antigen. Hemorrhagic cystitis due to adenovirus type 11 infection occurred 26 days after transplantation, and 17 days later the patients developed acute renal failure. A study of renal tissue obtained by needle biopsy showed degenerative and necrotic lesions, especially in the distal part of the nephron. By electron microscopy adenovirus type 11 particles were found in the nuclei of tubular cells and in cellular debris in tubular lumina. By immunofluorescence technique, granular immune deposits containing adenovirus type 11 related antigen(s), immunoglobulins, C3, and membrane attack complex (MAC) C5b-9 of the complement system were detected along the tubular basement membranes but not in glomeruli. The patient's IgG did not bind to normal human kidneys. These findings suggest that adenovirus type 11 directly induced acute tubular damage, and that the tubular immune deposits were formed "in situ" by viral antigens and circulating viral antibody.

Acute Kidney Injury↗

PubMed1993

Expression of growth hormone receptor gene in rat hypothalamus.

Growth hormone receptor (GHR) mRNA-expressing cells in the hypothalamus were observed using hybridization histochemistry in adult male rats. Digoxigenin-labeled cRNA corresponding to the extracellular part of rat GHR was used as a probe. Northern blotting analysis of hypothalamic total RNA from adult male rats revealed that the 4.5 kilobase (kb) transcript of the GHR gene corresponding to the GHR messenger RNA (mRNA) predominated over the 1.2 kb transcript corresponding to GH-binding protein mRNA. GHR mRNA-containing cells were observed in the arcuate nucleus (ARC), the periventricular nucleus (PeV), ventrolateral region of the ventromedial nucleus, the paraventricular nucleus and the supraoptic nucleus. To further understand the significance of the GHR gene expression in the hypothalamus, the effect of in vivo manipulation of GH on the somatostatin (SS) gene expression in the ARC and PeV, and the GRF gene expression in the ARC was observed among adult male rats using in situ hybridization histochemistry. Ten days after hypophysectomy, the SS mRNA level in the ARC as well as PeV was significantly lower than that in the respective nuclei of sham-operated control rats, while the GRF mRNA level in the ARC was significantly higher than that in the ARC of control animals. Subcutaneous injection of recombinant human GH (0.33 mg) to hypophysectomized rats every 12 h for 5 days restored the SS mRNA level in the ARC and PeV, and reduced the GRF mRNA level in the ARC to that of control animals. The data suggest that GH directly acts on the hypothalamic PeV and ARC, and alters the gene expression of SS and GRF.

Animals↗

PubMed1993

The renin-angiotensin-aldosterone system and hematologic changes during pulsatile and nonpulsatile cardiopulmonary bypass.

The effects of pulsatile and nonpulsatile cardiopulmonary bypass using a roller pump on levels of vasoactive hormones and hematologic changes were studied in 32 patients subjected to elective primary coronary artery bypass graft surgery. Seventeen patients had nonpulsatile perfusion (nonpulsatile group) and 15 patients had pulsatile perfusion (pulsatile group) during the period of cardiac arrest. Vasoactive hormones (plasma renin, angiotensin II, aldosterone, epinephrine, and norepinephrine) were measured in these patients. In order to clarify hematologic changes, plasma free hemoglobin, number of platelets, platelet factor 4, and beta-thromboglobulin were measured. There were no significant differences between the pulsatile and nonpulsatile groups with regard to vasoactive hormones and damage of platelets. In the pulsatile group, however, the rise of plasma free hemoglobin levels was significantly higher than that in the nonpulsatile group during and after cardiopulmonary bypass. We did not see the benefit of pulsatile perfusion using a roller pump on vasoactive hormones. Evidence of increased hemolysis with pulsatile flow was demonstrated in our cases.

Aldosterone↗

PubMed1993

Effect of parenteral administration of selected nutrients and central injection of gamma-globulin from antiserum to neuropeptide Y on growth hormone secretory pattern in food-deprived rats.

Serial changes in growth hormone (GH) secretory pattern during 72 h food deprivation and in response to refeeding after 72 h food deprivation were observed in adult male Wistar rats. In addition, the effects of intravenous administration of glucose, fatty acids or amino acids and central injection of gamma-globulin from antiserum to human neuropeptide Y (NPY) on the GH secretory pattern were examined in 72 h food-deprived rats. Rats were provided with chronic indwelling right atrial cannula and serial blood specimens were withdrawn via the cannula every 10 min using an automatic blood-sampling device. The GH secretory pattern was analyzed using a Pulsar computer program. In fed rats, episodic GH secretion with an amplitude exceeding 350 ng/ml occurred at 170-min intervals and intervening trough GH levels were less than 10 ng/ml. During the 72 h food deprivation, the amplitude of pulsatile GH secretion decreased progressively without altering the pulse frequency. When 72 h food-deprived rats were refed lab chow, the pulse frequency and the pulse amplitude increased rapidly and lasted for 6-8 h. Normal pulsatile GH secretion resumed by 10-12 h after refeeding following the 72 h food deprivation. To identify the specific nutrient (i.e. glucose, fatty acids or amino acids) serving as the signal causing restoration of the central drive to GH secretion, glucose solution (2 or 6 kcal), lipid emulsion (2 or 6 kcal) or amino acid solution (2 kcal) was administered intravenously to the 72 h food-deprived rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗

PubMed1993

Somatostatin reduces transcription of the growth hormone gene in rats.

To examine whether somatostatin (SS) exerts influences on the steady state levels of GH-releasing factor (GRF), the effect of SS on GH gene transcription was examined in rats. This approach was used because it has been shown that GRF stimulates GH gene transcription independent of GH release, and SS does not inhibit basal or GRF-stimulated GH gene transcription. Therefore, it is assumed that an effect of SS on GH gene transcription would be mediated by a change in GRF levels. Adult female Sprague-Dawley rats were provided with right atrial cannulae. Studies were performed using unanesthetized rats. Pituitary GH gene transcription was measured by transcription assay. An iv administration of antiserum to rat GRF 150 min previously significantly decreased GH gene transcription compared with that in control rats given normal goat serum. A continuous infusion of SS (300 micrograms/kg.h) via the cannula for 150 min significantly decreased GH gene transcription compared with that in control rats receiving 0.9% NaCl. When GRF (3 micrograms/kg.h) was given simultaneously with SS (300 micrograms/kg.h), GH gene transcription increased significantly compared with that in rats receiving SS infusion alone. After the withdrawal of SS infusion, GH gene transcription rapidly and significantly increased. The data suggest that SS reduces the steady state levels of GRF.

Animals↗

PubMed1993

Immunohistochemical localization of inhibin subunits in polycystic ovary.

The immunohistochemical localization of the alpha-, beta A-, and beta B-subunits of inhibin was examined in polycystic ovary (PCO). The granulosa cells in multicystic follicles in the PCO exhibited negative immunostaining for alpha-subunit and positive staining for beta A- and beta B-subunits. In contrast, the hyperplastic thecal cells exhibited distinct positive staining for alpha-, beta A-, and beta B-subunits. The luteinized stromal cells (stromal hyperthecosis) also exhibited positive staining for each inhibin subunit. The granulosa cells in the tertiary follicle exhibited negative staining for alpha-subunit and positive staining for beta A- and beta B-subunits. No staining for each inhibin subunit was observed in the thecal layer in the tertiary follicle. The present findings suggest that immunoreactive inhibin alpha-, beta A-, and beta B-subunits are present in hyperplastic thecal and stromal cells in PCO, and that granulosa cells in the follicular cyst of PCO might not produce inhibin, in contrast to the normal ovary.

Adult↗

PubMed1993

Effects of nitroglycerin on hemodynamics in dogs with experimentally inserted heartworms.

Hemodynamic effects of nitroglycerin were investigated in dogs with right ventricular failure including engorgement of the pulmonary artery and ascites induced by insertion of adult live heartworms into the pulmonary artery. The mean pulmonary arterial pressure (21.5 +/- 6.6 mmHg) in heartworm-inserted dogs 4 or 5 weeks after heartworm insertion were higher than that of control (4.2-7.1 mmHg). Nitroglycerin administered intravenously at doses of 3 or 10 micrograms/kg decreased pulmonary arterial pressure in these heartworm inserted dogs. This drug also decreased pulmonary vascular resistance and total systemic resistance with no effect on cardiac index and heart rate.

Animals↗

PubMed1993

Polymeric N-acetyl-D-glucosamine (chitin) induces histionic activation in dogs.

Analyses on the effects of polymeric N-acetyl-D-glucosamine (chitin), which was obtained from squid pen, on histiogenic activation in dogs were carried out with subcutaneous implants (5 x 5 cm2) of polyester non-woven fabric (NWF) supplemented with chitin (chitin group) and NWF (control group). These materials were implanted at 4 sites, on the lumbodorsal and lumbosacral subcutaneous tissues on both sides of the midline in each dog under general anesthesia. The implants and their surrounding tissues were isolated on post-implantation days (PIDs) 2, 4, 8, and 18 under general anesthesia. In the chitin group, the implant was organized gradually and its organization was completed on PID 18, when obvious angiogenesis toward the NWF was observed. On the other hand, in the control group, obvious angiogenesis toward the NWF was not observed macroscopically. Numbers of mononuclear (MN) and polymorphonuclear (PMN) cells concentrated around the implants on PID 2 were larger in the chitin than control group. In the chitin group, formation of granulating tissue around the implant was indicated on PID 4, whereas such a phenomenon was not observed in the control group. From these results, chitin accelerates the migration of MN and PMN cells to the NWF site with rapid follow-up organization of the NWF accompanied by angiogenesis.

Animals↗

PubMed1993

Application of polymeric N-acetyl-D-glucosamine (chitin) to veterinary practice.

The sponge-, cotton-, and flake-type remedies made of chitin (chitin-sponge, chitin-cotton, and chitin-flake, respectively), and non-woven fabric of polyester (NWF) composited with chitin (chitin-NWF) were applied to various types of trauma, abscess, surgical tissue defect and herniorrhaphy in 147 clinical cases including 72 dogs, 38 cows, 33 cats, 2 rabbits, one monkey and one horse. Chitin-sponge was applied in 30 cases as filling agent of surgical tissue defect, and in 25 cases of trauma, 31 cases of abscess as wound dressing or tissue defect filling agent. In 77 out of 86 cases (89.5%), good healing developed. When chitin-sponge was buried in surgical tissue defects due to oncotomy in 20 cases, recurrence of the tumor developed in one case on one month post-operatively, but was not recognized for 3-24 months in 19 cases. Chitin-NWF was applied in 2 cases of trauma and 12 cases of abscess as wound dressing or tissue defect filling agent, 6 cases as filling agent of surgical tissue defect, and 12 cases of umbilical hernia as prosthesis of suture site of hernia ring. In 28 out of 32 cases (87.5%), good healing developed. Chitin-cotton was applied in 8 cases of trauma and 12 cases of abscess as wound dressing or tissue defect filling agent. In 18 out of 20 cases (90.0%), good healing developed. Chitin-flake was applied in 9 cases of trauma as wound dressing or tissue defect filling agent. In 8 out of 9 cases (88.9%), good healing developed. In all cases, no side effects were observed.

Abscess↗

PubMed1993

Immunohistological study of LH-immunoreactive cells in the porcine anterior pituitary.

Anterior pituitary glands were removed from 18 sows at different stages of their estrous cycle to immunohistologically study the relationship between the stage of the estrous cycle and state of the luteinizing hormone (LH)-immunoreactive cells. The LH-immunoreactive cells were grouped into three types according to the amount of LH-immunoreactive granules stored in the cytoplasm (types A, B, and C). The number of LH-immunoreactive cells fluctuated along with the stage of the estrous cycle. In the 1st and the 6th stages, numerous LH-immunoreactive cells were observed (398 +/- 39, 391 +/- 60 cells/10(-2)mm2, respectively). In the 2nd stage, only a few cells were observed (6 cells/10(-2)mm2) and from the 3rd to the 5th stages, their number gradually increased.

Animals↗

PubMed1993

Impaired growth hormone secretion in VMH lesioned rats.

To investigate impaired growth hormone (GH) secretion in ventromedial nuclei (VMH) lesioned rats, we examined spontaneous plasma GH secretion, and plasma GH responses to arginine, clonidine and growth hormone releasing factor (GRF) under unanaesthetized and unrestrained conditions. Spontaneous GH secretion was blunted with 75% decrease of peak value in VMH lesioned rats, while it clearly existed in control rats. When rats were pre-treated with chlorpromazine (1-2 mg/kg, i.v.) which eliminates pulsatile GH secretion, no difference was observed in the plasma GH response to arginine (1 g/kg, i.v.) or to clonidine (100 micrograms/kg, i.v.) between VMH lesioned and control rats, but response to GRF (10 micrograms/kg, i.v.) was enhanced in the former animals. Administration of antiserum against somatostatin (1 ml) plus chlorpromazine significantly elevated the basal plasma GH level and GH response to arginine in control rats, but did not elevate them in VMH lesioned rats. These results suggest that reduction of both hypothalamic GRF and somatostatin release contribute to the impaired GH secretion in VMH lesioned rats. Reduction of somatostatin caused enhanced GH response to GRF and no increase in basal GH level with pre-treatment of antiserum against somatostatin. Reduction of GRF resulted in a failure to restore GH response to arginine with pre-treatment of antiserum against somatostatin. Reduction of both GRF and somatostatin caused blunted spontaneous GH secretion and normal GH response to arginine and clonidine.

Animals↗

PubMed1993

[A double-blind dose ranging study of weekly subcutaneous administration of rHuEPO (KRN5702) on post-phlebotomy anemia of patients scheduled for predeposit autologous blood transfusion (multicenter late PhII study)].

Preoperative blood reservation for autologous blood transfusion generally causes anemia. We performed a double blind controlled study to determine the optimal dose of subcutaneous rHuEPO (recombinant human erythropoietin, KRN5702) for preventing preoperative anemia due to blood reservation. Patients received KRN5702 subcutaneously once a week in a doses of 12000, 24000 or 36000 IU by a double blind technique. After storage of 1200 ml of their own blood right before surgery, their hemoglobin (Hb) averaged about 1 and was about 0.5 g/dl lower than the level before administration of KRN5702 in doses of 12000 and 24000 IU, respectively. This fall was significant. In patients receiving KRN5702 in a dose of 36000 IU, the level of Hb rose instead of a fall; Hb immediately before surgery was 1.1% higher than that before administration which, however, was not significant. This elevation indicates a possibility of abnormal elevation of Hb at this dose. The mean Hb value right before surgery was significantly lower in patients receiving 12000 IU KRN5702 than in patients of the other two groups. The recovery rate of Hb was an indicator to reflect improvement of anemic conditions, and increased as the dose increased after the blood reservation. The rate in the 12000 IU group was significantly lower than that in the other two groups; there was not much difference between the other two rates. We estimated that to reserve 1200 ml of autologous blood, 24000 IU of KRN5702 is adequate but not excessive. One patient receiving 24000 IU showed side effects including an elevation of body temperature, rash, and edema.

Adult↗