[Evaluation of assessment methods of obesity in children and adolescent].
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Adipose Tissue↗
Biomedical subjects
S Meng
Publications and source records attributed to S Meng.
[Expression of oncogene ras product p21 protein in the tissues of penis cancer and its surrounding tissues].
OBJECTIVE: To explore the clinical significance of expression of oncogene ras product p21 protein in the tissues of penis cancer and its surrounding tissues. METHOD: Oncogene ras product p21 protein in 32 cases of penis cancer, 32 cases of para-carcinoma tissues and in 16 cases of normal penis tissues were detected by immunohistochemical ABC method. RESULT: p21 protein expressed in 28 cases of penis cancer and 8 cases of para-carcinoma tissues, but it did not express in 16 cases of normal penis. Expression of p21 protein was related to pathological grading and clinical stage i. e, expression of p21 protein enhanced with the increasing of grading and stage. The positive cases of p21 protein expression in para-carcinoma tissue had worse impact on the prognosis of early recurrence and metastasis. CONCLUSION: p21 protein could be used as predictor of malignant potential and prognosis of penis cancer.
Humans↗
[Application of diode laser in the operation of retinal diseases].
OBJECTIVES: To evaluate the effects of diode laser photocoagulation applied on 43 cases of various kinds of fundus diseases with media opacity and on 40 cases during vitreo-retinal surgery. METHODS: Indirect ophthalmoscope laser photocoagulator was used for fundus diseases, and endolaser used during surgery. RESULTS: Vision was improved in 24.7% of the eyes, and was unchanged in 62.9% of the eyes. The neovascularization in proliferative diabetic retinopathy regressed in 78.3% of the eyes. All the retinal holes were closed. CONCLUSIONS: Diode laser can be used for treatment of some retinal diseases and in the operation. Good results can be obtained in the eyes with some extent of media opacity. No obvious side-effect was observed.
Adolescent↗
Butyrate inhibits colon carcinoma cell growth through two distinct pathways.
BACKGROUND: Dietary fiber and the resultant increase in colonic butyrate levels protect against colon carcinogenesis. Previous studies have shown that p21 and histone hyperacetylation are important in basal growth inhibition by butyrate. This study was designed to elucidate other mechanism underlying the butyrate effects on cell growth. METHODS: HT-29 colon carcinoma cells (standard medium or medium lacking serum) were treated with sodium butyrate (NaBu), epidermal growth factor (EGF), or both. Northern blot analyses were performed with cDNA probes specific for c-fos, c-jun, and actin. Cell growth was measured by 3H-thymidine incorporation. Enzyme-linked immunosorbent assay (ELISA) was used to quantify EGF receptor levels. RESULTS: Butyrate and serum starvation (SS) both induced a cell cycle withdrawal by 24 hours. In response to EGF treatment, SS cells exhibited a growth spurt and induced c-fos and c-jun proto-oncogene expression, whereas butyrate-treated cells exhibited minimal growth response to EGF. This relative unresponsiveness to EGF in butyrate-treated cells corresponded to a dramatic decline in EGF receptor levels when compared to untreated controls. CONCLUSIONS: Butyrate appears to inhibit colon cancer cell growth by two mechanisms, one involving histone hyperacetylation and p21 induction and the other related to impaired EGF-responsiveness.
Actins↗
[Clinical observation on juvenile-children type of Coats' disease].
OBJECTIVES: To observe the clinical features and the effects of management of juvenile-children type of Coats' disease and to approach the classification of the disease. METHODS: Fundus was examined and fundus fluorescein angiography (FFA) was made. Laser photocoagulation and cryotherapy were used for treatment. RESULTS: Compared to adult type of Coats' disease, this type was characterized by early onset, severe macular lesion, rapid progression and poor visual prognosis. The vision in 75.0% of eyes was unchanged, and in 19.2% of eyes was improved after treatment. CONCLUSIONS: Visual damage is more severe in this type. Early examination and early treatment are the key points to decrease visual deterioration. The classification of the disease is helpful in understanding the disease.
Adolescent↗
[Isolation of human herpes virus 6 from peripheral blood of renal transplant recipients].
One strain of the viruses was isolated from preipheral blood lymphocytes (PBL) of a renal transplant recipient. PBL isolated from blood samples were cocultured with the PHA actived cord blood lymphocytes (CBL). Two of twelve recipient's samples found cytopathic effect after 10 to 14 days. Examination of ultrathin-sections of the virus infected cells by electron microscope showed herpes-like virus particles. Detection of indirect immunofluorescences with McAbs against HHV-6 was positive in the infected cells.
Graft Rejection↗
[Determination of chrysophanol in semen Cassiae by HPLC].
A HPLC method for the determination of chrysophanol in Semen Cassiae was investigated. The result showed that this method was simple, specific and accurate. The recovery was 99.0% and relative standard deviation was 1.8%. The method is useful for the quality control of this medicinal meaterial.
Anthraquinones↗
[The application of intracoronary stenting to type B and C lesions of coronary arteries].
Intracoronary stenting was introduced to 4 lesions due to coronary arterial dissections and to 2 lesions owing to elastic recoil of coronary arteries and stent placement was directly performed in 6 complex coronary lesions after 12 target lesions from 7 patients with Types B and C lesions of coronary arteries had been pre-dilated with balloon. Coronary angiography showed that dissections and recoils had disappeared. Target lesions were well dilated and blood flow was well improved in all cases, one patient was complicated with hemorrhage which was cured quickly. During the follow-up period of 1 to 24 months, another case had recurrence of angina after 2 months of stenting which could be controlled with anti-angina agents. These indicate that intracoronary stenting had good therapeutic effect and is considered as a safe, fast and effective method in dealing with acute complications of coronary artery after routine PTCA.
Aged↗
[Short-term observation on the therapeutic effects of percutaneous balloon mitral valvuloplasty].
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Adult↗
Thyroid hormone and the gut: selective transcriptional activation of a villus-enterocyte marker.
BACKGROUND: Thyroid hormone (T3) is an important regulator of gut mucosal growth, differentiation, and barrier function, but its mechanism of action in the gastrointestinal tract is largely unknown. The present studies were carried out to define the molecular mechanisms by which T3 alters gut gene expression. METHODS: In vivo: Adult, male, Sprague-Dawley rats were given three daily injections (intraperitoneal) of either saline solution or 30 micrograms/kg triiodothyronine. Small intestinal tissues were harvested, and Northern blot analyses were performed by using specific radiolabeled cDNA probes. In vitro: HT-29 cells were transfected with reporter plasmids and treated with or without T3, and chloramphenicol acetyltransferase activity was measured. RESULTS: The T3-induced changes in enterocyte gene expression occurred in villus enterocytes and not in crypt cells and were independent of food intake. Northern analyses with an intron-specific probe revealed that the T3 induction in intestinal alkaline phosphatase (IAP) expression occurs at the level of transcription. Transient transfection assays revealed no T3-induced changes under basal conditions but marked increases (sixfold, p < 0.001) when a T3-receptor (TR beta-1) plasmid was cotransfected. Furthermore, T3 was found to induce greater IAP reporter gene activity in differentiated (+ sodium butyrate) compared with undifferentiated HT-29 cells. CONCLUSIONS: T3 induces IAP expression at the level of gene transcription. Both in vivo and in vitro, IAP transcriptional activation occurs to a greater extent in differentiated enterocytes than in undifferentiated crypt cells. Transactivation of the IAP gene by T3 is mediated via a DNA cis-element(s) located within the 2.4 kb segment present in the reporter gene.
Animals↗
Cellular growth state differentially regulates enterocyte gene expression in butyrate-treated HT-29 cells.
Enterocyte differentiation occurs at the crypt-villus junction through the transcriptional activation of cell-specific genes, including the brush-border enzyme intestinal alkaline phosphatase (IAP) and the microvillar structural protein, villin. Based upon previous in vivo studies demonstrating that IAP and villin are differentially affected by alterations in epithelial growth state, we examined the regulation of these two genes in an in vitro model of enterocyte differentiation. HT-29 cells were maintained in DMEM + 10% FCS and treated with sodium butyrate to induce enterocyte differentiation. Cell count and [3H]thymidine measurements confirm that sodium butyrate causes a marked decrease in cellular growth state, consistent with the differentiation process. Northern blot analyses revealed time- and dose-dependent increases (P < 0.001) in steady-state IAP and villin mRNA levels. The increases in both IAP and villin expression were dependent upon the presence of one or more newly synthesized proteins. Post-confluence and serum starvation were used to cause cell cycle withdrawal prior to the differentiation process. As predicted from our previous in vivo data, villin expression was unaffected by changes in cellular growth state, whereas IAP expression was dramatically diminished under hypoproliferative conditions. We conclude that early withdrawal from the cell cycle alters the course of enterocyte differentiation, specifically decreasing IAP expression.
Alkaline Phosphatase↗
Differential cloning of novel intestine-specific genes whose expression is altered under conditions of villus atrophy.
Atrophy of the small intestinal villi occurs in a variety of disease states and is associated with diarrhea, malabsorption, and impaired barrier function. We have previously demonstrated that villus atrophy is associated with an increase in lactase and a decrease in intestinal alkaline phosphatase gene expression. Given these changes in enterocyte phenotype with villus atrophy, we speculated that there may be other intestine-specific genes whose expression is altered as a function of epithelial growth state. We have employed two molecular techniques in order to identify and clone complementary DNAs (cDNA) which are differentially expressed in atrophic compared to normal small intestinal mucosa. In differential cDNA library (+/-) screening, duplicate filters of a normal jejunal cDNA library are hybridized with radiolabeled cDNA probes from either atrophic or control tissues. Comparisons of the intensities of hybridized clones allows for the identification of differentially expressed gene products. In the mRNA differential display system, RT-PCR is used to randomly amplify mRNA species. Similar to cDNA library screening, comparisons of radiolabeled bands on a polyacrylamide sequencing gel allow for the identification of differentially expressed genes. Using these methods, we have identified a novel cDNA, called D9, which appears to be expressed exclusively in the intestinal mucosa. Northern analyses have confirmed that the expression of the D9 mRNA is dramatically decreased under conditions of villus atrophy, suggesting an underlying relationship with epithelial growth state. DNA sequence analysis (GenBank) reveals no identity to previously cloned genes.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Small bowel adaptation: counterregulatory effects of epidermal growth factor and somatostatin on the program of early gene expression.
BACKGROUND: Small intestinal crypt cell proliferation is essential to the normal renewal of the epithelium, as well as the adaptive responses that follow resection or injury. The present studies were designed to elucidate the molecular mechanisms by which epidermal growth factor (EGF) and somatostatin interact to regulate crypt cell proliferation. METHODS: Rat crypt (IEC-6) cells were maintained in Dulbecco's modified Eagle's medium plus 10% fetal calf serum and treated with EGF (10 or 100 ng/ml) or somatostatin (0.5 microgram/ml). Cell counts were done to examine the effects on cellular growth, and Northern blot analyses were carried out by using complementary DNA probes corresponding to various protooncogenes. RESULTS: EGF caused a 41% increase in cellular growth, an effect that was almost completely blocked by pretreatment (30 minutes) with somatostatin. EGF led to dramatic increases in c-fos (greater than 20-fold), c-jun (2-fold), and jun B (3-fold) gene expression at 30 minutes, consistent with the previously characterized immediate-early gene response in IEC-6 cells. Somatostatin alone had no effects on protooncogene levels, but pretreatment with somatostatin resulted in a marked inhibition (80%, p < 0.001 in all cases) of the EGF-induced increases in protooncogene expression. CONCLUSIONS: Somatostatin inhibits the EGF-induced protooncogene expression in IEC-6 cells. The somatostatin inhibition of immediate-early gene expression lends support to its role as a negative growth regulator in intestinal epithelia and indicates that its effect occur at an upstream site in the cellular growth response.
Adaptation, Physiological↗
Pattern of rat intestinal brush-border enzyme gene expression changes with epithelial growth state.
Enterocyte growth and differentiation occur simultaneously within the epithelium, but little is known regarding any relationship between these two processes. Four rat models of small intestinal epithelial hypo- and hyperplasia (neonatal ontogeny, fasting/refeeding, hypo-/hyperthyroidism, and bombesin treatment) were used to study the regulation of enterocyte gene expression in relation to epithelial growth state. Mucosal scrapings, as well as crypt and villus cell populations, were subjected to Northern blot analyses using radiolabeled cDNA probes corresponding to lactase, intestinal alkaline phosphatase, villin, ornithine decarboxylase (ODC), and the actin control. In all four models, the hypoplastic (atrophic) condition is characterized by high levels of lactase and low levels of the 3.0-kb intestinal alkaline phosphatase mRNA, whereas under hyperplastic conditions this pattern is reversed. The changes in intestinal alkaline phosphatase and lactase are qualitatively similar along the longitudinal axis of the intestine and are proportional to the degree of hyperplasia, as verified by ODC mRNA levels. Furthermore, the crypt-villus axis of differentiation is maintained regardless of epithelial growth state. In conclusion, the pattern of brush-border enzyme gene expression changes as a function of epithelial growth state, indicating a previously unrecognized degree of plasticity to the state of enterocyte differentiation.
Alkaline Phosphatase↗
[The diagnosis and treatment of insulinoma: an analysis on 20 cases].
From 1967 to 1994, a total of 20 pathologically proved benign insulinoma cases were treated in our hospital. Tumors were solitary in 19 cases, and multiple in 1, with diameter ranging from 0.5 to 3.0 cm. Diagnosis was made by autopsy in one and by laparotomy in 19. All 18 cases except for the one dying of pancreatic fistula postoperatively during the same hospitalization were followed up for 1 to 18 years without recurrence. The authors recommended calcium infusion test as the standard method of diagnosis, analysed the false diagnosis in one patient, and discussed operative experience on tumors deeply buried in the head of the pancreas.
Follow-Up Studies↗
[A new method for qualitative determination of astragaloside].
A multi-function column has been devised for qualitative determination of astragaloside in Yuanheng-yitai Chutang Tablets. The result of experiment shows that the method is convenient fast and stable.
Chromatography, Thin Layer↗
Immediate-early gene expression in EGF-stimulated intestinal epithelial cells.
The mammalian small intestinal epithelium is a continuously renewable population of cells which arise from a proliferative zone of undifferentiated stem cells within the crypts. Epidermal growth factor (EGF) is thought to play a role in the maintenance and proliferative response of the intestinal epithelium. In order to investigate the early changes in intestinal gene expression which occur in response to a mitogenic stimulus, we performed studies in two different cell lines (IEC-6 and HT-29), both of which have characteristics of intestinal crypt cells. Cells were grown in DMEM + 10% FCS at 37 degrees C/5% CO2 and treated with either EGF (10 ng/ml) and/or cycloheximide (CHx) (10 micrograms/ml) for various times. Northern blot analyses were performed on total RNA using 32P-labeled cDNA probes corresponding to various protooncogenes. Our results showed that EGF treatment caused rapid increases in c-fos, c-jun, and junB expression (P < 0.001) in both cell lines. c-fos and c-jun followed similar time courses, peaking at 30 min, whereas junB levels plateaued at 1 hr. The magnitude and time course of protooncogene induction by EGF were similar in the two cell lines. A dose-response experiment indicated peak EGF effects at 10 ng/ml. CHx treatment resulted in greater and more prolonged increases in protooncogene expression when compared to EGF alone, indicating that protein synthesis is not required for protooncogene induction.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Temporal pattern of rat small intestinal gene expression with refeeding.
Studies were carried out to elucidate the molecular mechanisms underlying small intestinal epithelial growth. Adult rats were fasted for 4 days and then refed a chow diet for up to 48 h. Histological examination confirmed the sequential occurrence of mucosal atrophy and hyperplasia. Northern blot analyses of RNA derived from small intestinal mucosal scrapings revealed a striking pattern of alterations in the expression of two different categories of genes. There were very early increases in the expression of c-fos and c-jun, reflecting the mitogenic response to refeeding that occurs within the crypt compartment. Studies using the protein synthesis inhibitor cycloheximide suggest that c-fos and c-jun are part of the "immediate-early" response of the small intestine. At later time points after the refeeding stimulus, differential changes occurred in the expression of the brush-border enzymes, lactase, and intestinal alkaline phosphatase (IAP). Refeeding caused a decrease in lactase gene expression and an increase in the expression of the 3.0-kb IAP mRNA species, reflecting a return of the villus phenotype to the normal fed state. Thus we have demonstrated a complex and temporally related pattern of gene expression within the small intestinal epithelium upon refeeding. The results provide insight into the relationship between the processes of intestinal growth and differentiation.
Animals↗