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Biomedical subjects

S Matthews

Publications and source records attributed to S Matthews.

140 records · Page 8Linked to original sources
PubMed1985

The distribution of cells of the monocytic-lineage in reactive lymph nodes and non-Hodgkin's lymphomata. Characterization using protein histochemistry, lectin binding and monoclonal antibodies.

In this study cells of putative monocytic origin have been investigated in lymph nodes, using a battery of cytochemical investigations on fixed and frozen tissue. In reactive lymph nodes involved in non-Hodgkin's lymphomata six types of presumed monocyte-derived cells have been identified on the basis of morphology and/or cytochemistry. These cells can be divided into two distinct groups. Certain cell types have distinctive distributions in reactive and non-Hodgkin's lymphomata and this taken in conjunction with their morphology and staining properties may give important clues to their function.

Antibodies, Monoclonal↗
PubMed1985

A study of the relationship between neurological function and serum vitamin E concentrations in patients with cystic fibrosis.

A patient with cystic fibrosis and undetectable serum vitamin E concentrations is described who developed a progressive spinocerebellar syndrome and pigmentary retinopathy with abnormal somatosensory and visual evoked potentials (SSEPs and VEPs). In order to assess the relationship between neurological function and serum vitamin E concentrations in cystic fibrosis, 29 unselected patients who had no neurological symptoms were examined neurologically. Ten were randomly selected for neurophysiological assessment by recording SSEPs and VEPs. Electroretinograms (ERGs) were also performed in five cases. The findings were correlated with serum vitamin E concentrations which were unknown to the neurological investigators prior to completion of the study. Only one patient had definite reflex and sensory abnormalities, and the remaining 28 were clinically normal. The ERG was abnormal in two cases, one of whom had abnormal VEPs. SSEPs were normal in all 10 cases. Twenty six patients had serum vitamin E concentrations below the normal range. In two of the three patients who had definite neurological or electrophysiological abnormalities serum vitamin E concentrations were below the median value for the whole group.

Adult↗
PubMed1984

Joint report of the Second International Workshop on Lymphocyte Alloantigens of the Horse, held 3-8 October 1982.

The Second International Workshop on Lymphocyte Alloantigens of the Horse was held 3-8 October 1982. At this workshop, the 6 specificities identified at the first workshop were confirmed and an additional 5 new specificities were identified and given workshop nomenclature. Four of the new specificities, products of the ELA locus, were named ELA-W7, W8, W9, and W10. An additional specificity, designated ELY-2.1, is the product of a locus independent of the ELA locus. Cell isolation methods were compared at this workshop. Technical variation in methods clearly affected reactivity of many reagents. However, when highly selected reagents were used, antigen assignment did not differ regardless of the cell isolation method. Based on the comparison of methods, isolation procedures in which thrombin was used were more effective than those relying on carbonyl iron or slow centrifugation.

Animals↗
PubMed1975

The glue that holds.

Explore the source record for details and available documents.

Community Health Services↗
PubMedDate not supplied

Conservative management of minimally displaced radial fractures in three horses.

Minimally displaced, spiral, radial fractures were diagnosed in three adult horses. Two horses had open fractures and in one horse the fracture was closed. A diagnosis was made on radiographs taken 24 h, 5, and 9 days after the fracture. There was minimal displacement of the fracture fragments despite the considerable time which had elapsed between fracture and diagnosis and an unfavourable prognosis with internal fixation, so conservative therapy was the preferred method of treatment. One horse developed a sequestrum and draining sinus, which was treated surgically under general anaesthesia 3 months after diagnosis of the fracture. Other complications were minimal and transient. Two horses were being used for pleasure riding 12 months after fracture and one horse was pasture sound at 8 months.

Animals↗
PubMedDate not supplied

The haggis tolerance test in Scots and Sassenachs.

To find out if the Scottish national dish, haggis, contributes to the high incidence of coronary heart disease in Scotland the lipaemic effect of a meal of 200 g of haggis was measured in six Scottish and 10 Sassenach men. The Scots had higher fasting cholesterol and triglyceride concentrations and a lower proportion of high density lipoprotein cholesterol than the Sassenachs. Four subjects were found to have hyperlipoproteinaemia, which had been unrecognised previously. Serum cholesterol concentrations did not change after haggis was eaten (mean dose 2.6 g/kg body weight). Serum concentrations of triglycerides increased by 51% at 90 minutes in the Sassenachs but were unaltered in the Scots. There were no serious adverse effects. This study shows that Scots have higher lipid concentrations than Sassenachs but seem to be resistant to the lipaemic effect of haggis. The haggis tolerance test may be useful in Sassenachs.

Adult↗
PubMedDate not supplied

Identification of a major latent nuclear antigen, LNA-1, in the human herpesvirus 8 genome.

OBJECTIVES: Human herpesvirus 8 (HHV-8) is strongly associated with all forms of Kaposi's sarcoma (KS) and with primary effusion lymphomas (PEL). KS patients' sera are immunoreactive against discrete nuclear localizing antigens in PEL cell lines. This study sought to identify and characterize these nuclear localizing proteins. STUDY DESIGN/METHODS: KS patients' sera were used to screen a cDNA expression library derived from a PEL cell line (BCP-1) latently infected with HHV-8. RESULTS: An HHV-8-specific cDNA clone was isolated. It encoded one partial and two complete open reading frames (ORFs): ORF 73, ORF 72 (v-cyclin), and K13, respectively. The immunodominant epitope was mapped to the C-terminal domain of ORF 73. Analysis with the KS patients' sera of HEK 293 cells transfected with a clone encompassing the complete coding region of ORF 73, ORF 72, and K13 gave a nuclear immunofluorescence pattern similar to that observed in BCP-1 cells. Western blot analysis with KS patients' sera of transfected HEK 293 cells revealed an immunoreactive protein of 220 to 230 kD that was similar to that observed previously in PEL cell lines. After induction of lytic replication of HHV-8 in BCP-1 cells with n-butyrate, we observed a major reduction in the expression of an ORF 73-specific 6.6-kb mRNA, indicating that this region is under latent control. CONCLUSIONS: These data identify a region of HHV-8 encoding for a major immunoreactive latent nuclear antigen (LNA-1), analogous to the Epstein-Barr virus latent nuclear antigens.

Amino Acid Sequence↗
PubMedDate not supplied

Acarosan and the Acarex test in the control of house dust mite allergens in the home.

House dust mites are believed to be major triggers for allergic disease in atopic individuals. As part of a programme controlling dietary and aero-allergen exposure in high-risk infants, an acaricidal foam and powder (Acarosan) was applied to bedroom and main living room carpets, as well as upholstered furniture, on four occasions in the first year of life. Dust was assayed for mite antigen (Der p1) and these results compared with the semi-quantitative assay of guanine content (Acarex Test). After nine months mean Der p1 levels had decreased by 70% in the treatment group. Proportionally, the greatest fall occurred in those items that had the highest initial mite antigen content. The Acarex score does show a correlation with Der p1 levels, but cannot replace antigen assay when accurate data is required. A chemical acaricide may help reduce house dust mite antigen levels, but is not by itself sufficient to reduce levels below that considered critical for sensitisation.

Allergens↗
PubMedDate not supplied

The effects of economic conditions and access to reproductive health services on state abortion rates and birthrates.

The effects that such factors as wages, welfare policies and access to physicians, family planning clinics and abortion providers have on abortion rates and birthrates are examined in analyses based on 1978-1988 state-level data and longitudinal regression techniques. The incidence of abortion is found to be lower in states where access to providers is reduced and state policies are restrictive. Calculations indicate that decreased access may have accounted for about one-quarter of the 5% decline in abortion rates between 1988 and 1992. In addition, birthrates are elevated where the costs of contraception are higher because access to obstetrician-gynecologists and family planning services is reduced. Economic resources such as higher wages for men and women and generous welfare benefits are significantly and consistently related to increased birthrates; however, even a 10% cut in public assistance benefits would result in only one birth fewer for every 212 women on welfare. Economic factors showed no consistent relationship with abortion rates.

Abortion, Legal↗