Search PubMed⌕ Search

Biomedical subjects

S Matsutani

Publications and source records attributed to S Matsutani.

At least 73 records · Page 4Linked to original sources
PubMed1989

Distribution of neuropeptidelike immunoreactivities in the guinea pig olfactory bulb.

The distribution of neuropeptidelike immunoreactivities in the adult guinea pig olfactory bulb was studied immunohistochemically with antisera raised against neurotensin (NT), substance P (SP), methionine-enkephalin-Arg6-Gly7-Leu8 (ENK), somatostatin (SOM), neuropeptide Y (NPY), and cholecystokinin-8 (CCK). In the main olfactory bulb, NT-like immunoreactive (NT-IR) neurons were found among periglomerular cells. In addition, a few periglomerular cells showed ENK-like immunoreactivity. Granule cells displaying SP- or ENK-like immunoreactivities and short axon cells with SOM- or NPY-like immunoreactivities were observed in the deeper half of the granule cell layer. SOM-IR short axon cells were also seen in the external plexiform layer. Dense NT- or NPY-IR fibers were distributed in superficial lamina of the granule cell layer, and sparse SP- or CCK-IR fibers were found in the glomerular layer. In the accessory olfactory bulb, some mitral, periglomerular, and granule cells showed NT-like immunoreactivity. SP- or ENK-IR granule cells were also observed. These results are discussed in relation to laminar organization of the olfactory bulb. The most characteristic features of peptide distribution in guinea pigs, as compared with that of rats in previous studies, were the relative abundance of NT-IR structures and the lack of SP- and CCK-IR juxtaglomerular and tufted cells.

Animals↗
PubMed1989

Laminar and segregated distribution of immunoreactivities for some neuropeptides and adenosine deaminase in the superior colliculus of the rat.

The distribution and morphology of adenosine deaminase, substance P, leucine-enkephalin, corticotropin-releasing factor, and calcitonin gene-related peptidelike immunoreactive cells and fibers throughout the superior colliculus of the rat were examined by means of the unlabelled-antibody peroxidase-antiperoxidase method. Adenosine deaminase immunoreactive cells were found in the stratum opticum and lower stratum griseum superficiale; substance P immunoreactive cells were localized to the upper stratum griseum superficiale, and calcitonin gene-related peptide immunolabelled neurons were situated in deeper strata. Substance P, leucine-enkephalin, and calcitonin gene-related peptide immunoreactive fibers were distributed similarly in their lamination and in their patchlike organization. Corticotropin-releasing factor immunoreactive fibers were observed evenly throughout all the strata and were fewer in the stratum griseum superficiale. These findings suggest that, as in afferent modules and segregated efferents of the mammalian superior colliculus, the cells and fibers containing neuroactive substances and neuroactive substance-related enzymes also show a segregated and laminar distribution.

Adenosine Deaminase↗
PubMed1989

Adenosine deaminase containing fiber pathway from the superior colliculus to the lateral posterior nucleus of the rat.

Presence of a projection containing adenosine deaminase (ADA)-like immunoreactivity from the stratum opticum (SO) to the dorsomedial portion of the lateral posterior nucleus of the thalamus (LPN) of the rat was demonstrated using a method combining retrograde tracing by horseradish peroxidase (HRP) and immunohistochemistry for ADA. In the caudal two thirds of the LPN, a clearly delimited ADA-like immunoreactive fiber plexus was located. Injection of HRP into the lateral posterior nucleus labeled many neurons in the medial portion of the SO where medium-sized neurons with ADA immunoreactivity were concentrated. Simultaneous immunostaining showed that some of the HRP-labeled neurons are ADA-positive.

Adenosine Deaminase↗
PubMed1989

Establishment of gene transfer systems for and construction of the genetic map of a marine Vibrio strain.

Two gene transfer systems were established for a marine bacterium, Vibrio sp. strain 60. One was generalized transduction with a newly isolated bacteriophage, As3, and the other was conjugal gene transfer by the use of newly constructed transposon-facilitated recombination (Tfr) donors. As3 transduced various chromosomal markers at frequencies of 10(-4) to 10(-6). Tfr donors, which were constructed by introducing transposon Tn10 into both plasmid RP4 and the chromosome, mediated the polarized transfer of chromosomal genes from the sites of Tn10 insertion on the chromosome. By means of these gene transfer systems, a genetic map of the vibrio chromosome was constructed.

Bacteriophages↗
PubMed1988

High-performance liquid chromatography of the anti-tumour agent triethylenethiophosphoramide and its metabolite triethylenephosphoramide with sodium sulphide, taurine and o-phthalaldehyde as pre-column fluorescent derivatization reagents.

The method described is based on the reaction of triethylenethiophosphoramide (ThioTEPA) and triethylenephosphoramide (TEPA), through their ethyleneimine groups, with sodium sulphide, taurine and o-phthalaldehyde to give fluorescent products, and separation of the derivatives by reversed-phase high-performance liquid chromatography. The method was successfully applied to the determination of ThioTEPA and TEPA in rabbit plasma samples after clean-up with an Extrelut 3 column. The recoveries of ThioTEPA and TEPA from plasma were 66.1-80.3% and the limits of determination in plasma were ca. 10 and 20 ng/ml, respectively.

Animals↗
PubMed1988

Neuropeptide- and neurotransmitter-related immunoreactivities in the developing rat olfactory bulb.

The development of neuropeptide and neurotransmitter-related immunoreactivities in the rat olfactory bulb were investigated immunohistochemically by using antisera raised against substance P (SP), cholecystokinin-8 (CCK), neurotensin (NT), leucine-enkephalin or methionine-enkephalin-Arg6-Gly7-Leu8 (ENK), somatostatin (SOM), neuropeptide Y (NPY) and tyrosine hydroxylase (TH). Results obtained for the adult olfactory bulb confirmed previous observations, except for SP-like immunoreactive (SP-IR) granule cells in the main olfactory bulb (MOB) and NT-IR neurons around the modified glomerular complex (MGC) (Teicher et al., Brain Res. 194:530-535, 1980). SP-, CCK- and NT-IR neurons were observed in the MOB of the rat fetus. SP-IR neurons also appeared in the accessory olfactory bulb (AOB). Among them, NT-IR neurons in the MOB and SP-IR neurons in the AOB were observed on embryonic day 16. SP- and CCK-IR neurons in the MOB appeared on embryonic day 18. Most of these neurons were presumed to be projecting neurons. SOM-, NPY-, ENK- and TH-IR neurons appeared in the newborn rats. The number and intensity of immunostaining of these neurons continued to increase with age, producing the adult pattern, except for NT-IR neurons in the MGC and SP-IR neurons in the mitral cell layer of the AOB, which were more numerous and intensely stained in young animals.

Animals↗
PubMed1988

Portal hemodynamics in patients with gastric varices. A study in 230 patients with esophageal and/or gastric varices using portal vein catheterization.

The hemodynamic features of gastric varices are not well documented. The purpose of this study was to investigate the nature of hepatofugal collateral veins, their origins, the direction of blood flow in the major veins and collateral veins, and portal venous pressure. To this end, 230 patients, mostly cirrhotic, who had esophageal or gastric varices, or both, demonstrated by endoscopy were investigated by portal vein catheterization. The findings were correlated with endoscopically assessed degrees of varices. Gastric varices were seen in 57% of the patients with varices due to portal hypertension. In most of the patients with advanced gastric varices, esophageal varices were minimal or absent. When patients with gastric varices were compared with those having predominantly esophageal varices, it was found that advanced gastric varices were more frequently supplied by the short and posterior gastric veins, they were almost always associated with large gastrorenal shunts, and portal venous pressure in patients with large gastric varices was lower. Chronic portal systemic encephalopathy was more common in patients with large gastric varices due to hepatofugal flow of superior mesenteric venous blood in the splenic vein than in patients with predominantly esophageal varices. Thus, the hemodynamics in patients with large gastric varices are distinctly different from those in patients with mainly esophageal varices, and such differences seem to account for the differing incidence of chronic encephalopathy and variceal bleeding.

Adult↗
PubMed1988

Pretectum and accessory optic system in the filefish Navodon modestus (Balistidae, Teleostei) with special reference to visual projections to the cerebellum and oculomotor nuclei.

The fiber connections of the accessory optic system (AOS) were investigated in a balistid fish, Navodon modestus (filefish), by means of horseradish peroxidase (HRP) and degeneration methods. Following injections of HRP into the corpus cerebelli, neurons in two retinal recipient nuclei, the area pretectalis pars dorsalis (APd) and area pretectalis pars ventralis (APv), were labeled retrogradely. In addition, a few neurons near the nucleus of the accessory optic tract (nAOT) were labeled. These neurons have dendrites extending into nAOT. Neurons in APv were also labeled by HRP injections into the oculomotor complex (nIII). However, no neurons were labeled in APd or nAOT. A few neurons in the lateral part of APv were labeled by HRP injections into the abducens nucleus (nVI). Three nuclei of the AOS, APd, APv and nAOT, were shown to receive tectal projections by the Fink-Heimer method. Thus, APv receives retinal and tectal projections, and in turn projects to corpus cerebelli, nIII and nVI. Specific efferent connections of the AOS in teleosts are discussed from phylogenetic aspects.

Afferent Pathways↗
PubMed1988

Pulsed Doppler in the diagnosis of small liver tumours.

Doppler ultrasound detection of the blood flow associated with liver tumours was studied in primary hepatocellular carcinoma as well as in metastatic liver cancer and haemangioma. Doppler signals were detected from 48 of 55 hepatocellular carcinomas (87.3%), seven of 25 metastatic liver cancers (28.0%) and four of 30 haemangiomas (13.3%). The waveforms of Doppler signals were divided into two types: the pulsatile wave, which was detected from hepatocellular carcinoma (in 35 of the 48 with Doppler signals) and metastatic liver cancer (in all seven with positive signals), and the continuous wave, which was seen from hepatocellular carcinoma (41 out of 48) and haemangioma (in all four with signals). In six patients with hepatocellular carcinoma who underwent transcatheter arterial embolization, the pulsatile wave detected before therapy disappeared immediately thereafter and it is possible that this type of wave originates from tumour vessels. In the study of small, hypoechoic, mass lesions appearing in liver cirrhosis, such signals were also demonstrated, even in eight of 10 small hepatocellular carcinomas less than 2 cm in diameter, whilst they were not detected from nine regenerative nodules related to cirrhotic change. In conclusion, the Doppler ultrasound method may be a useful technique in detecting blood flow within liver tumours and may offer the possibility of a differential diagnosis of small tumours.

Adult↗
PubMed1987

High-performance liquid chromatographic method for determining trichothecene mycotoxins by post-column fluorescence derivatization.

The method described here is based on a separation of deoxynivalenol, nivalenol and fusarenon-X on a C18 column using aqueous acetonitrile, and successive post-column fluorescence derivatization involving an alkaline decomposition to form formaldehyde and modified Hantzsch reaction with methyl acetoacetate and ammonium acetate (lambda ex = 370 nm and lambda em = 460 nm). By this method, 5-10 ng of the standard trichothecenes could be determined. By employing a clean-up procedure with a florisil column and a Sep-Pak CN cartridge, 61.4-96.9% recoveries were obtained for deoxynivalenol and nivalenol added to corn, wheat and barley at concentration levels of 0.05-1 ppm.

Chromatography, High Pressure Liquid↗
PubMed1987

Isolation and characterization of IS elements repeated in the bacterial chromosome.

Shigella sonnei contains repetitive sequences, including an insertion element IS1, which can be isolated as double-stranded DNA fragments by DNA denaturation and renaturation and by treatment with S1 nuclease. In this paper, we describe a method of cloning the IS1 fragments prepared by the S1 nuclease digestion technique into phage M13mp8 RFI DNA. Several clones contained IS1, usually with a few additional bases. We isolated and characterized five other repetitive sequences using this method. One sequence, 1264 base-pairs in length, had terminal inverted repeats and contained two open reading frames. This sequence, called IS600, showed about 44% sequence homology with IS3 and was repeated more than 20 times in the Sh. sonnei chromosome. Another sequence (named IS629, 1310 base-pairs in length), which was repeated six times, was found also to be related to IS3 and thus IS600. Two other sequences (named IS630 and IS640, 1159 and 1092 base-pairs in length, respectively), which were repeated approximately ten times, had characteristic terminal inverted repeats and contained a large open reading frame coding for a protein. The inverted repeat sequences of IS630 were similar to the sequence at one end of IS200, a Salmonella-specific IS element. The fifth sequence, repeated ten times in Sh. sonnei, had about 98% sequence homology with a portion of IS2. The method described here can be applied to the isolation of IS or iso-IS elements present in any other bacterial chromosome.

Chromosomes, Bacterial↗
PubMed1987

Activation of the isthmo-optic neurons by the visual Wulst stimulation.

The visual Wulst (VW) in the avian telencephalon is thought to be an avian equivalent of the mammalian striate cortex. Effects of electrical stimulation of VW were studied in the isthmo-optic nucleus (ION) of the Japanese quail by extracellular recording. Most ION neurons examined were activated by VW stimulation, and their response latencies ranged from 12 to 27 ms (mean +/- S.D. = 17 +/- 4 ms, n = 67). Thus, this study suggested that the avian 'visual cortex' could modulate some retinal function through the ION neurons.

Animals↗
PubMed1986

Cytoarchitecture, synaptic organization and fiber connections of the nucleus olfactoretinalis in a teleost (Navodon modestus).

Cytoarchitecture, synaptic organization and fiber connections of the nucleus olfactoretinalis (NOR) in a teleost, Navodon modestus, have been studied light- and electron-microscopically using an HRP or HRP-degeneration combined method. Following HRP injections into the optic nerve, most contralateral and a few ipsilateral neurons in the NOR were labeled. There are two types of neurons in NOR. Type I neurons have a medium-sized spindle-shaped soma with a round nucleus, and type II neurons have a large oval soma with an invaginated nucleus and contain cored vesicles (80-130 nm in diameter). Afferent terminals which form synaptic contacts with cell bodies of NOR neurons were classified into 3 types according to their morphological characteristics; S, F1 and F2 terminals. S terminals originated in ipsilateral area ventralis telencephali pars supracommissuralis (Vs). These terminals contain both spherical and cored vesicles, and make synaptic contacts with both type I and type II neurons. F1 terminals, which originated in ipsilateral area dorsalis telencephali pars posterior (Dp), are large in profile, and contain flat vesicles and mitochondria with irregularly arranged cristae. These terminals make synaptic contacts only with type I neurons. F2 terminals are small in profile, and contain flat vesicles, cored vesicles and small mitochondria with regularly arranged cristae. F2 terminals make synaptic contacts with both type I and type II neurons. The functional significance of NOR and the relationship between NOR and the ganglion of the nervus terminalis are discussed.

Animals↗