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Biomedical subjects

S Matsui

Publications and source records attributed to S Matsui.

At least 325 records · Page 18Linked to original sources
PubMed1986

Serial changes of plasma cortisol levels during various types of asthmatic responses due to allergen inhalation.

To observe possible changes in adrenal function during asthmatic attacks due to allergen inhalation, plasma cortisol levels were measured serially in asthmatic patients who showed immediate asthmatic responses, late asthmatic responses, and dual asthmatic responses were compared with their diurnal levels. There were slightly different patterns of cortisol responses among these groups. In the five patients who showed late asthmatic responses alone, cortisol levels fell significantly during late-type attacks although the decrease was quantitatively small. In the six patients who showed immediate asthmatic responses alone, cortisol levels tended to increase slightly during immediate-type attacks. Cortisol levels in the group with dual asthmatic responses increased slightly during immediate attacks and significantly decreased during late-type attacks, although the decrease was also small.

Adolescent↗
PubMed1986

[Experimental study on the pathogenesis of acute ulceration in obstructive jaundice--with reference to gastric mucosal blood flow].

We prepared obstructive jaundice models in rats in order to study the mechanism of acute ulceration in obstructive jaundice centering on impediments to gastric wall blood flow and changes in gastric mucosal NA and PGE2 when the rats were subjected to water immersion restraint stress. The results were: In the obstructive jaundice 2 weeks group, when subjected to water immersion restraint stress, gastric mucosal NA reached a dried up stage from the incipient stage, causing gastric mucosal impediments at the same time, showing a significant decrease of gastric mucosal PGE2. Intragastric pH was at a similar level of excessive acidity in all groups; gastric acid is believed to be a secondary factor promoting ulceration. Gastric mucosal PGE2 showed a significant decrease coinciding with the increase in ulceration index, being a possible factor of ulceration; it is also presumed to regulate gastric wall blood flow alternatively with gastric mucosal NA. Pre-treatment with PGE2 prior to loading stress resulted in a decrease in gastric wall blood flow being significantly controlled. The administration of PGE2 brought about an improvement in gastric wall blood flow and a consequent increase in gastric mucosal NA, being judged effective for acute ulceration in obstructive jaundice.

Acute Disease↗
PubMed1986

[Magnetic resonance imaging of experimental cerebral ischemia: correlations between NMR parameters and water content].

Recent studies on proton NMR imaging revealed its remarkable sensitivity for detecting cerebral ischemia. Since proton NMR reflects the distribution and state of water in the brain, an NMR imager becomes a sensitive in vivo detector of brain edema developing soon after the energy state is compromized by ischemia. To further clarify the usefulness of NMR imaging to characterize the ischemia-induced changes, correlations between T1 and T2 relaxation times and water content of the normal and ischemic rat and gerbil brain were studied by means of both spectroscopic and in vivo imaging methods. In the spectroscopic experiment on excised rat brain (cortex, white matter, hippocampus and thalamus for normal and ischemia-laden brain), T1 and T2 relaxation times and water content were determined. The ischemic insult was induced for 60 min by the method of Pulsinelli followed by 60 min of reperfusion. All of the T1, T2 and water content significantly increased in the ischemic tissue. Gray-white difference was evident in T1 and T1 was linearly correlated with the water content of the tissue. T2 was by far prolonged in the ischemic tissue compared with the increase in the water content, showing greater sensitivity of T2 for detection of ischemia. In the imaging experiment, coronal NMR imaging at 0.5 tesla was performed employing proton density-weighted saturation recovery (TR = 1.6 s, TE = 14 ms), T1-weighted inversion recovery (TR = 1.6 s, TI = 300 ms, TE = 14 ms) and T2-weighted spin echo (TR = 1.6 s, TE = 106 ms) pulse sequences.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1986

[Experimental study on efficacy of cimetidine and 16,16-dm PGE2 to liver regeneration in prevention of stress ulcer after partial hepatectomy in the cirrhotic rat].

Stress ulcer is a common phenomenon after hepatectomy for cirrhotic liver. We examined experimentally the influence of cimetidine and 16,16-dm PGE2 dosaged as counter-measures against stress ulcer on liver regeneration after partial hepatectomy for cirrhotic liver. Using cimetidine and 16,16-dm PGE2, there was a noticeable suppression of the onset of water immersion stress ulcer, and both a decline in the blood flow in the wall of the stomach and a drop in PH in the stomach were inhibited after partial hepatectomy for cirrhotic liver. The group given 16,16-dm PGE2 after partial hepatectomy had a higher rate of DNA synthesis as compared with the group which had only partial hepatectomy. No difference was seen between the group given cimetidine and the only partial hepatectomy groups. The group given indomethacin before partial hepatectomy for cirrhotic liver had a noticeable decrease in mitotic index 30 hours after partial hepatectomy. However, the group given both indomethacin and 16,16-dm pGE2, showed some recovery of the mitotic index.

16,16-Dimethylprostaglandin E2↗
PubMed1985

Quantitative solubilization of nonhistone chromosomal proteins without denaturation using zwitterionic detergents.

Of three kinds of commercial zwitterionic detergents [SB 12, SB 14, and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (Chaps)], SB 12 and Chaps were more useful than SB 14 because of high solubility and less interference with protein assay. Efficiency for protein solubilization at pH 6-9 was higher for SB 12 than for Chaps with either calf thymus chromatin or rat liver nuclei. At pH 9 and ionic strength (I) = 0.35, 1% SB 12 and 1% Chaps were capable of solubilizing about 70% and about 47% of total proteins in rat liver nuclei, respectively. Core histones in rat liver nuclei were extracted to a lesser extent with Chaps than with SB 12. DNA-dependent RNA polymerase and isopeptidase activities were barely inactivated by 1% Chaps at pH 8-9, but isopeptidase activity was inhibited by 0.3% SB 12. These facts indicate that whereas SB 12 is effective for solubilization of whole nuclear proteins, Chaps is suitable for the selective extraction of nonhistone chromosomal proteins without denaturation.

Animals↗
PubMed1985

Comparison of subcellular proteins of normal prostate, benign prostatic hypertrophy, and prostatic cancer: presence of BPH-associated nonhistone proteins.

Proteins in the cytosol, postnuclear particulate, and nuclear fractions from seven specimens of normal prostate from bladder cancer patients, 14 specimens of benign hypertrophic prostate (BPH), and three specimens of cancerous prostate were analyzed and compared by SDS-polyacrylamide slab gel electrophoresis. Abundant protein species in the cytosol fractions were 60K (species having a molecular weight of about 60,000) and 42K; their relative contents were about 35% for 60K and about 12% for 42K. In the postnuclear particulate fraction, 42K was the most abundant (about 10% of the total). The contents of these major protein species were similar in specimens of normal and diseased prostates. In addition, there are marked similarities in the electrophoretic patterns for all the protein (24-29 species) in the cytosol and postnuclear particulate fractions of the human prostate, except for four minor species in the cytosol fraction. Of the nuclear proteins, the content of core histones (H2A, H2B, H3, and H4) was fundamentally similar among all the specimens, whereas the content of H1 histone was different from one specimen to another. The most remarkable and significant difference was that the 42K-NHP (nonhistone protein having a molecular weight of about 42,000), 55K-NHP, and 190K-NHP concentrations were significantly higher in BPH than in normal and cancerous prostates.

Chromosomal Proteins, Non-Histone↗