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Biomedical subjects

S Matsuda

Publications and source records attributed to S Matsuda.

At least 613 records · Page 34Linked to original sources
PubMed1986

[Urine polyamine in patients with malignant urological diseases using a polyamine-test enzyme kit].

Using a polyamine-test enzyme kit, the urine polyamine concentration was determined in 74 patients with malignant urological disease (12 with renal cell cancer, 13 with pelvic-ureter cancer, 24 with bladder cancer and 25 with prostate cancer), 7 patients with BPH, 20 patients with benign urological disease and 20 normal subjects. The urine polyamine level was significantly elevated in all the patients with any malignant urological disease compared to normal subjects. It was also significantly high in the patients with BPH. Defining the mean +/- 3SD (= 50 mumole/g Cr.) of 20 normal subjects as an upper limit, slightly higher levels not exceeding 100 mumol/g Cr. were frequently observed in the patients with BPH or with benign urological disease. Setting the upper limit at 100 mumole/g Cr., the positive rate amounted to 33% (low stage 17%) in renal cell cancer, 23% (low stage 14%) in pelvic ureter cancer, 13% (low stage 0%) in bladder cancer and 4% (low stage 0%) in prostate cancer. The positive rate was low especially in low stage cases.

Diamines↗
PubMed1986

[Results of mass screening by automobile for cancer of the cervix uteri in Fukui Prefecture in the past ten years].

The total number women examined by the screening car staff from 1974 to 1983 was 120,860. The number of people examined increased from 3.9% in 1974 to 6.2% in 1983, but is still low. The percentage of people requiring detailed examination was 0.77%-2.02%, and the rate of those, actually examined in detail was 86.3%-97.7%. The rate of detection of carcinoma was 0.08-0.3%. The ratio of dysplasia, CIS and invasive carcinoma was 1.3:1.7:1.0 in the first half of the 1974-78 period and changed to 6.1:2.5:1.0 during the 5 years from 1979 to 1983. Regarding the number of examinations required before detecting CIS and invasive carcinoma, all subject with a more advanced than stage Ib carcinoma of the cervix uteri were detected at the time of the initial examination. As to stage Ia of carcinoma of the cervix, 3 out of 31 subjects were detected at the second visit, 28 subjects at the first visit; 126 out of 146 subjects with CIS at the first visit, and 6 at the third visit or later. The results of cytodiagnosis were, in 72 cases of the class IV, dysplasia was found in 8.7%, CIS in 56.9%, invasive carcinoma in 12.1%, false positive in 22.2%, while in 51 cases of class V, dysplasia was found in 1.9%, CIS in 45.1%, invasive carcinoma 47.1% and false positive in 5.8%. CAI in Fukui Prefecture calculated as the sum of clinic and automobile health examinations, was 152 in 1982, 148 in 1983, 177 in 1984.

Adult↗
PubMed1985

Growth of the initial dendrite of early differentiated interneurons in the zona intermedia of the embryonic chick cervical cord: a morphometric study with the Golgi method.

From stages 24 to 45 of the chick embryo, the longest dendrite of each interneuron in the zona intermedia was measured in the upper cervical cord prepared by the Golgi method, the measuring being done exclusively on the large neurons at each stage to follow the dendritic growth of neurons homogeneous in their time of origin. The longest dendrite grew at a rate of 92-184 micron per day at stage 24, and thereafter its growth rate gradually slowed down to 4 micron per day at stage 36. Already at stage 27, the interneurons located deep in the mantle layer reached with their longest dendrite the marginal layer which was the site of synaptic contact in early developmental stages. Thus, the initial rapid growth of the dendrite could be regarded as highly effective for the early formation of synaptic contact.

Animals↗
PubMed1985

The fine structure of the ganglia of the guinea-pig trachea.

The parasympathetic ganglia of the guinea-pig trachea have been investigated by scanning and transmission electron microscopy. They are covered by a continuous perineurium and connective tissue is found between the neural elements. Blood vessels inside the ganglia have continuous endothelia and are sometimes accompanied by pericytes and a sheath of perineurial cells. Individual neuronal cell bodies and large processes are almost completely covered by a thin layer of satellite cells, except for very small areas that directly face the basal lamina and connective tissue space. Nerve fibres are also completely and individually ensheathed by Schwann cell processes; naked fibres are not found. In some regions of the nerve cell body, there are complex interdigitations between short neuronal processes and satellite cells. Large differences in the size of neurons may indicate the presence of different neuronal populations. Nerve endings containing mainly small clear vesicles are the most common type, and these form synapses on dendrites, but some profiles have many large granular vesicles. These ganglia resemble other parasympathetic, sympathetic and sensory ganglia and not the enteric ganglia. However, an unusual feature of the cytoplasm of the satellite and Schwann cells is the abundance of 10 nm intermediate filaments.

Animals↗
PubMed1985

A case of adult T-cell leukemia with metastatic calcification.

We report a case of adult T-cell leukemia with increased uptake in both lungs which was detected by a bone scan using 99mTc-MDP. This finding is thought to have been caused by the metastatic calcification which is associated with ectopic parathormone production.

Adult↗
PubMed1985

Effects of sodium n-butyrate on alpha-fetoprotein and albumin secretion in the human hepatoma cell line PLC/PRF/5.

The in vitro effects of sodium n-butyrate (butyrate) on the growth, morphology and secretion of alpha-fetoprotein (AFP) and albumin by the human heptoma cell line PLC/PRF/5 were studied. Butyrate caused a marked reduction in the growth rate, colony forming efficiency in soft agar and de novo synthesis of DNA as well as remarkable morphological changes including cell enlargement, flattening and a decreased number of nucleoli. Secretion of AFP was reduced during culture with butyrate, while that of albumin was increased. The requirement of de novo protein synthesis for the increase in albumin and decrease of AFP by butyrate was demonstrated by inhibition studies with cycloheximide. These results suggest that butyrate caused the hepatoma cells to acquire in vitro properties that are considered to be more consistent with normal liver cells.

Albumins↗
PubMed1985

Kinetic studies on lymphocytes labelled with indium 111-oxine in patients with chronic lymphocytic leukaemia.

The kinetics of 111Indium-oxine-labelled autologous blood lymphocytes were studied in normal subjects and 10 patients with chronic lymphocytic leukaemia (CLL). In both normal and leukaemic subjects, intravascular survival of lymphocytes was characterized by two exponential decreases, the first being a rapid one and the second slow phase. T 1/2 of the second phase for normal T lymphocytes (52.0 +/- 3.2 h: mean +/- SEM) was longer than that for B lymphocytes (31.6 +/- 2.8 h). T 1/2 of the second phase in patients with CLL was 15.1 to 192.6 h, which correlated well with the clinical stage of CLL by the Rai classification. Body surface scanning revealed prominent splenic accumulation of labelled cells in normal individuals and patients with CLL. Lymph node visualization was recognized in all patients with T-cell CLL; however, there was no visualization in 8 of the 9 patients with B-cell CLL. The longer survival time and splenic and lymph node visualization suggest that an expansion of the extravascular lymphocyte pool may be important in the pathophysiology of CLL.

Aged↗
PubMed1985

Effects of corticosteroid and 1,24R-dihydroxy-vitamin D3 administration on lymphoproliferation and autoimmune disease in MRL/MP-lpr/lpr mice.

The pharmacological effects of prolonged administration of a corticosteroid, betamethasone, and active vitamin D3 [1,24R-(OH)2D3] on lymphoproliferation and autoimmune disease of MRL/MP-lpr/lpr (MRL/1) mice were examined. Relatively high doses of betamethasone (0.25 mg/kg/day) prevented lymphoproliferation, reduced serum levels of anti-dsDNA, anti-ssDNA, and anti-poly (ADP-ribose) antibodies, and brought about clinical improvement, such as reduced proteinuria and diminution of skin lesions. It is noteworthy that not only did prevention of lymphoproliferation occur, but also recovery of the Lyt-2+ T cell subset in the thymus and the spleen was observed. The administration of 1,24R-(OH)2D3 (0.1 microgram/kg/day) similarly prevented proteinuria, and produced recovery of a Lyt-2+ subset in the thymus.

Animals↗
PubMed1985

[Pharmacological studies on pinacidil, a new anti-hypertensive agent. 1. Antihypertensive effects in SHR and DOC/saline hypertensive rats].

Antihypertensive effects of pinacidil (PND) were compared with those of hydralazine (HDL) and nifedipine (NFD) by determining the systolic blood pressure of the caudal arteries of conscious SHR and desoxycorticosterone (DOC)/saline hypertensive rats at temperatures between 29 degrees C to 30 degrees C using a newly developed tail cuff method. 1) A dose-dependent antihypertensive effect of PND in male SHR was observed in a dose exceeding 1 mg/kg, p.o. Comparison of antihypertensive effects during 6 hr after oral administration of antihypertensive agents showed that relative potencies of HDL and NFD to PND (1.0) were approximately 0.8 and 0.3, respectively. 2) Although no difference was observed in the antihypertensive effect of HDL between male and female SHR, these effects by PND and NFD were more potent in female SHR. 3) No tolerance was observed in the antihypertensive effect of PND by the once a day oral administration for 5 weeks in male SHR and DOC/saline hypertensive rats. Dose-dependent antihypertensive effects of PND in doses above 1 mg/kg were always observed during experimental periods. 4) In spite of the potent antihypertensive effects, pathological changes such as hyperplasia and hyalinization of arterial walls in kidneys in DOC/saline hypertensive rats were not improved by continuous administration of PND or HDL.

Administration, Oral↗
PubMed1985

[Pharmacological studies on pinacidil, a new antihypertensive agent. 2. Studies on the hypotensive mechanism of pinacidil].

Effects of pinacidil (PND) on the blood pressure of anesthetized cats and contraction of guinea-pig isolated hearts and blood vessels were compared with those of hydralazine (HDL) and nifedipine (NFD). PND showed a dose-dependent hypotensive effect and decrease of total peripheral resistance in a dose above 0.3 mg/kg (p.o.) in anesthetized cats. However, no involvement of the autonomic nervous system was presumed in the hypotensive effect of PND due to studies on autonomic responses. Although negative inotropic and chronotropic effects of PND in the isolated guinea-pig atria and heart (Langendorff method) were slight, PND caused a coronary vasodilation at a dose of 1 micrograms. PND inhibited the norepinephrine (NE) contracture of isolated guinea-pig thoracic aorta and portal vein at 10(-6)-10(-5) M, but concentrations of 10(-5)-10(-4) M were required for the inhibition of K contracture. In the isolated thoracic aorta, HDL markedly inhibited NE contracture, while NFD inhibited K contracture. These results suggest that the hypotensive effect of PND is more closely associated with the inhibition of Ca2+ influx caused by the receptor activation of Ca2+ release from intracellular storage sites than membrane potential-dependent Ca2+ influx.

Animals↗
PubMed1985

[Clinical and statistical study on recent cases of suprapubic prostatectomy].

Results of operation were analyzed statistically in 123 patients who underwent suprapubic prostatectomy for benign prostatic hypertrophy from 1974 through 1981. Age of the patients ranged from 48 to 89 years and the mean age was 70.1 years. The average amount of blood loss during operation was 590 ml. Amount of blood loss in patients operated under epidural anesthesia was less than that in the patients operated under general anesthesia. Furthermore, significant correlation was observed between amount of blood loss and the weights of enucleated prostate glands. The average weight of enucleated prostate glands was 33.4 g and the weight increased as the patient's age increased. Histological examination of the enucleated specimens revealed occult carcinoma in 4.1% of the patients. Association of bladder neck sclerosis was observed in 27% of the patients whose prostate weighed less than 20 g. Significance of prophylactic vasectomy for the prevention of postoperative epididymitis was considered to be low. Incidence of postoperative bacteriuria was higher in patients who had bacteriuria preoperatively than those without preoperative bacteriuria. Therefore, eradication of preoperative bacteriuria seemed important in the prevention of postoperative bacteriuria. No significant correlation was observed between postoperative residual urine volume and the weight of the enucleated prostate glands.

Aged↗