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Biomedical subjects

S Marklund

Publications and source records attributed to S Marklund.

At least 73 records · Page 4Linked to original sources
PubMed1976

A comparison between the common type and a rare genetic variant of human cupro-zinc superoxide dismutase.

Human cupro-zinc superoxide dismutase is polymorphic in northern Sweden. The genetic variant type has a lower mobility at electrophoresis in alkaline buffer. The enzyme was isolated from erythrocytes from one of the rare homozygotes and its properties compared to those of the common type. The isoelectric point of the variant was higher (4.85) than that of the common type (4.7). Small differences in amino acid composition were found but no definite amino acid substitutions could be pointed out. The molecular weights were equal as judged from electrophoreses in polyacrylamide gels in the presence of dodecylsulphate. The ultraviolet spectra were similar. Parameters related to the active site of the enzyme were very similar; i.e. specific activity and sensitivity to inhibition by cyanide and by H2O2. These parameters, especially the latter two, differ widely between species. Both enzymes were stable for weeks at neutral pH at 37 degrees C, whereas the common type was significantly more stable at pH 4 and pH 11 and also at incubation in neutral buffer at 70 degrees C. It appears that the active site of the variant is conserved whereas the stability of the enzyme is affected.

Amino Acids↗
PubMed1975

[What is THX?].

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Antigens↗
PubMed1975

Lactoperoxidase activity in human milk and in saliva of newborn infants.

Human milk and saliva from newborn infants were analyzed for their content of lactoperoxidase and thiocyanate. The activity of lactoperoxidase in infant saliva was variable but generally higher than that found in calf saliva. In contrast, the activity in human colostrum was low ( approximately 5%) compared with that found in cow's milk. The enzyme was resistant to gastric juice. Thiocyanate was demonstrated in infant saliva in concentrations about one-third of that in adult saliva. The amounts of lactoperoxidase and thiocyanate in infant saliva are quite sufficient to inhibit bacterial growth in in vitro systems. The importance of this system in vivo has not yet been demonstrated. The availability of this system to both newborn calves and humans (in calves provided largely by colostrum and in human babies by saliva) might be indirect evidence of its importance.

Adult↗