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Biomedical subjects

S Mansueto

Publications and source records attributed to S Mansueto.

At least 91 records · Page 5Linked to original sources
PubMed1980

[Further observations on the use of counterimmunoelectrophoresis (CIEP) on cellulose acetate membrane (Cellogel) in the diagnosis of visceral leishmaniasis (author's transl)].

Technique of counterimmunoelectrophoresis (CIEP) on cellulose acetate membrane (Cellogel) is described for diagnosis of (human and canine) visceral leishmaniasis (VL). Various lots of antigen were grossly obtained from the liquid phase (10, 20 and 30 tubes respectively) of cultures of Leishmania donovani by repeated freezing and thawing. Sera from patients (and, in a few cases, dogs) with confirmed VL, other parasitic and non-parastic diseases (especially blood disorders and hepatosplenomegaly) and from blood donors were tested. Positive results were obtained in 91-93% (according to various lots) of the patients with VL. All sera from infected dogs gave positive results. No precipitin lines were detected in the control sera. Antigens from 30 (or 20 tubes) showed better results with regard to the evidence of the precipitin lines. Clarity of the precipitin bands appears to be in relation to: 1. the protein concentration of the antigens; 2. the antibody levels of the sera. CIEP on cellulose acetate membrane combine the features of good sensitivity, specificity and speed of performance and appear available for use also in epidemiological research.

Animals↗
PubMed1979

[The complement fixation test in leishmaniasis. Comparison between an antigen extracted from leishmania cultures and one extracted from BCG].

A comparison between a BCG antigen and various batches of an antigen extracted at different times from leishmania cultures in the fixation of complement reaction is reported. A better response was obtained with BCG (further confirmation on a larger series is being sought), whereas the leishmania antigens were difficult to prepare and their batch titres were not constant.

Animals↗
PubMed1979

[Sporadic persistence of amebic hepatitis].

A case of classical colliquative amoebic hepatitis, documented with aspiration of abundant amoebic pus by means of hepatocentesis and treated with chloroquine and haematin, is reported. This type of amoebiasis is rare nowadays, it is pointed out. Present-day clinico-epidemiological features are outlined: reduction in official cases, reversal of the ratio between colitis and hepatitis (the latter is on the increase), disappearance of acute dysenteric forms, less tendency to colliquative development in cases where the liver is involved, an involvement which is seen most frequently with an atypical chronic-type imprint. Mention is finally made of the reasons behind this cliniqo-epidemiological shift.

Chloroquine↗
PubMed1979

[Counterimmunoelectrophoresis on cellulose acetate membrane with a commercial lyophilized antigen in diagnosis of human hydatidosis].

A very simplified method of crossed over electrophoresis (CIEP) was employed with a lyophilized commercially produced antigen (previously submitted to several freezing and thawing) and cellulose acetate membrane, for the diagnosis of human hydatidosis. Results are as follows: active hydatidosis (surgically confirmed): number of sera tested 35; positive 32 (91%). Sera of patients with parasitic and non parasitic illnesses (especially malignancies of liver and lung) did not show any precipitin lines. The pattern of immunoprecipitation is characteristically in the form of an "upper lip" or a thick and undulating "streak". The very thin arcs of precipitation which are occurring in some control sera, are to be considered negative. These results indicate that with regard to the rule of the "three S" (specificity, sensitivity and simplicity) the method appears sufficiently satisfactory.

Antibody Formation↗
PubMed1978

[Counterimmunoelectrophoresis in the diagnosis of visceral leishmaniasis].

Technique of counter current immunoelectrophoresis (C.I.E.P.) was employed for the diagnosis of V.L. using an antigen grossly extracted (by means of repeated freezing and thawing) from culture of Leishmania. 6 lots of antigen were prepared - in various time - at the same way. Positive results are obtained in V.L. from 83.3 to 94% (according to various lots of antigens). Few false positivity (from 1.4 to 8.8%) are obtained in sera from patients with other diseases (especially) cirrhosis and blood disorders. No positivity in controls (blood donors). The reproducibility of results appear satisfactory. Our results suggested that C.I.E.P., rapid and less sophisticated test, can be applied for the diagnosis of V.L. But false positivity and false negativity limit the value of these test.

Antibodies↗
PubMed1978

[Clinical problems in current malaria. Considerations apropos of 2 cases].

Two cases of malaria imported from West Africa by two sailors (falciparum and vivax respectively) are reported. It is pointed out that nowadays malaria is no longer indigenous to Sicily but has to be imported; an increasing number of cases of malaria are being reported world-wide in relation to increased traffic volumes, particularly air traffic. This means growing importance of imported pathology for which a new training strategy is required (with regard to young physicians who at present are quite uniformed of the problem). Doctors and travellers should also be informed of the risk of contracting malaria.

Adult↗
PubMed1975

[The complement fixation test with BCG in the diagnosis of leishmaniasis].

The utility of complement-fixation test with an antigen from commercially lyophilized BCG was investigated in the diagnosis of leishmaniasis. The following results were obtained: Visceral leishmaniasis: 4 positivity on 5 cases; Oriental sore: 3 positivity on 6 cases; Canine leishmaniasis: 6 positivity, at high level, and 2 negativity; Other diseases: 5 positivity, on 17 sera of tbc; No positivity in sera from patients with various diseases, in control and in normal dogs. The results are compared with those quoted in medical literature and discussed. The meaning of the reaction is also briefly discussed, and the hypothesis of the presence of partigens between BCG and leishmania, or presence of autoantibodies is prospected.

Animals↗