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Biomedical subjects

S Majewski

Publications and source records attributed to S Majewski.

At least 91 records · Page 5Linked to original sources
PubMed1990

Natural cell-mediated cytotoxicity against various target cells in patients with epidermodysplasia verruciformis.

Natural cell-mediated cytotoxicity of peripheral blood mononuclear cells was studied in eight patients with epidermodysplasia verruciformis induced by human papillomaviruses specific for epidermodysplasia verruciformis and in five patients with epidermodysplasia verruciformis-induced exclusively by human papillomavirus type 3. Nine patients with various cutaneous warts and 25 age- and sex-matched healthy persons were control subjects. Natural cell-mediated cytotoxicity against both K-562 erythroleukemic and Sk-v cells was in the normal range in patients with epidermodysplasia verruciformis induced by epidermodysplasia verruciformis-specific human papillomaviruses and in patients with cutaneous warts. The lysis of both targets, however, was significantly decreased in patients with the form of epidermodysplasia verruciformis associated with human papillomavirus type 3. Experiments with normal keratinocytes and with keratinocytes isolated from a malignant lesion bearing human papillomavirus type 5 genomes showed that the latter were susceptible to lysis by the peripheral blood mononuclear cells of healthy persons and of patients with cutaneous warts. Lysis of keratinocytes in epidermodysplasia verruciformis, however, was strongly reduced in patients with epidermodysplasia verruciformis induced by specific human papillomaviruses. This reduction was not associated with a decrease in anti-K-562 natural cell-mediated cytotoxicity. Our results suggest that in patients with epidermodysplasia verruciformis induced by disease-specific human papillomaviruses, there is reduced natural cell-mediated cytotoxicity against epidermodysplasia verruciformis keratinocytes.

Adult↗
PubMed1989

Abrogated NK-cell lysis of human papillomavirus (HPV)-16-bearing keratinocytes in patients with pre-cancerous and cancerous HPV-induced anogenital lesions.

Natural-cell-mediated cytotoxicity against K-562 erythroleukemic cells and human papillomavirus (HPV)-16 harboring Sk-v keratinocytes was tested in 38 age- and sex-matched healthy volunteers and in patients with HPV-induced benign and malignant anogenital lesions: 9 persons with HPV-16-induced bowenoid papulosis (BP), 8 with anogenital carcinomas (5 with HPV-16- or 33-associated squamous-cell carcinomas of Bowen's type and 3 with HPV-6-associated Buschke-Loewenstein verrucous carcinomas) and 12 with HPV-6-induced condylomata acuminata. Both K-562 and Sk-v cells were killed by a non-adherent CD16+ subset of PBMC as revealed by cell fractionation on the basis of their adherence to plastic and by treatment with Leu-IIb monoclonal antibody (MAb) and complement. "Cold" target competitive assays demonstrated that both cell types inhibited lysis of labelled Sk-v cells. In patients with BP and anogenital carcinomas induced by HPV-16 or 33, there was a significant (at least at p less than 0.01) decrease of Sk-v cell lysis as compared with the healthy control group. Anti-K-562 activity was not affected. In patients with anogenital carcinomas the degree of Sk-v lysis was decreased in proportion to the duration of lesions (correlation coefficient-r = -0.79). Neither anti-K-562 nor anti-Sk-v cytotoxicities were significantly affected in patients with condylomata and with HPV-6 associated verrucous carcinomas. Short-term (3 hr) pre-incubation of normal PBMC with sera from patients with BP and HPV-16-associated anogenital carcinomas resulted in significant inhibition of their ability to lyse Sk-v cells. Lysis of K-562 cells remained unaffected. In patients with carcinomas, the suppressive effect of sera was associated with a lowering of the ability of their PBMC to lyse Sk-v cells (r = -0.79). In patients with longer tumor persistence, the suppressive effect of serum was proportionally higher (r = 0.86).

Adult↗
PubMed1989

Effects of systemic etretinate treatment on natural cytotoxicity, immune angiogenesis and neutrophil adherence in patients with various forms of psoriasis.

The effects of etretinate treatment on natural killer cell activity, on angiogenic capability of peripheral blood mononuclears and on neutrophil adherence were studied in 20 patients with various forms of psoriasis. In all forms of active psoriasis, etretinate was found to affect first the angiogenic reaction and PMN adherence, whereas NK cell activity usually normalized only after a long-term therapy. The earliest normalization of all three parameters was noticed in pustular psoriasis, and it was correlated with the clinical improvement. In two cases of palmo-plantar pustular psoriasis, in 5 cases of arthropathic variety, and in 5 cases of common psoriasis, the normalization preceded clearing of the skin lesions.

Adult↗
PubMed1989

Effects of psoriatic patients' neutrophils and sera on angiogenic capability and natural cytotoxicity of normal human peripheral blood mononuclear cells.

We studied the effects of products released from adhering neutrophils and serum factors from patients with psoriasis on angiogenic capability and natural cytotoxicity of normal human mononuclear cells. In active disseminated and guttate psoriasis, in which polymorphonuclear granulocytes were most adherent to the human vascular endothelium, supernatants from adhering neutrophils only slightly affected natural killer cell activity and angiogenic capability of normal human mononuclears. In peripherally spreading, plaque-like psoriasis, characterized by lower than in guttate psoriasis increase in granulocyte adherence, both neutrophil factors and patients sera markedly decreased NK cell activity and enhanced angiogenic capability of normal human peripheral blood mononuclear cells. The results suggest that neutrophil factors may affect some immune responses in patients with psoriasis.

Adolescent↗
PubMed1988

Modulatory effect of sera from scleroderma patients on lymphocyte-induced angiogenesis. II. Effector cells for the enhancing effect of acroscleroderma patients' sera.

In our previous study, using the modified lymphocyte-induced angiogenesis assay, we found that incubation of normal human peripheral blood mononuclear cells (PBMCs) with sera from patients with acroscleroderma, but not with diffuse scleroderma, markedly enhanced their angiogenic capability. The results of the present work suggest that this enhancement is mediated by lymphocytes bearing receptors for the Fc portion of IgG and belonging mainly to the CD4+ T-cell subset.

Adult↗
PubMed1988

Theophylline-resistant and theophylline-sensitive "active" and "total" E rosette-forming lymphocytes in patients with systemic scleroderma.

Using the E rosette test and its modification with theophylline, we have studied T regulatory lymphocytes in various forms of systemic scleroderma. Mean percentages of active rosette-forming cells (ARFC) as well as the fraction resistant to theophylline incubation (ARFC-res) were significantly decreased, irrespective of the variety of the disease, compared to the age-matched controls. Late ("cold") rosette-forming fractions were unimpaired. The theophylline-sensitive fraction of total rosette-forming cells (TRFC-sens), which contains mainly cells from the suppressor circuit, was found to be lowered in all patients groups studied, whereas the ARFC-sens fraction was significantly decreased only in patients with diffuse scleroderma over 50 years of age, in whom there was a tendency to a more severe course, as manifested by pronounced systemic organ involvement. The lowered values of E rosette tests were found in a majority of SSc patients and were correlated with the appearance in the sera of factors capable of inhibiting ARFC formation by normal human peripheral blood T lymphocytes. Normal values of the E rosette test were related to the presence in the patients' sera of factors stimulating ARFC formation by normal lymphocytes. We surmise from the results that in SSc patients the T-cell defect is not only restricted to T suppressor cells but also refers to the active theophylline-resistant fraction containing mainly T inducer and T cytotoxic cells.

Adult↗
PubMed1988

[Results of toothlessness treatment by calottic method in the Cracow modification. Clinical studies].

The purpose of the paper was to control the new method of teeth positioning in complete dentures based on the modification of callottic method in clinical practice. Clinical studies depended on occlusion and articulation estimation, examination of denture statics, control of occlusion height and registration of the patients' subjective experiences. They concerned 81 toothless patients divided into 2 groups according to the specific classification and wearing complete dentures in which the teeth had been positioned according to the modified method and Gysi-Fischer' method. In the groups of patients under examination that had teeth positioned according to the modified method significantly better results of the values estimated than in comparative groups have been achieved. It has been found that using the modified method smooth OA surface of artifical teeth is obtained without necessity of its correction satisfactory denture statics is obtained and rehabilitation of mastication function and adaptation to the dentures take place in a short period of time.

Denture Design↗
PubMed1988

[Prosthetic management in cases of generalized destruction of dental crowns].

Disturbances in stomatognathic system (ss) emerging as a result of generalized destruction of dental crowns of noncarious origin have been described in the article. Principles of two-stage rehabilitation of ss have been presented. Three own cases creating examples of two-stage prosthetic rehabilitation have been described.

Dentures↗
PubMed1988

Occurrence of large groups of mast cells in subcutaneous connective tissue in the mouse.

Skin from the mouse trunk together with panniculus adiposus and panniculus carnosus and, separately, trunk muscles, were fixed, stained with Astra blue at pH 1.0, made translucent in methyl salicylate and whole-mounted. In the connective tissue on the surface of panniculus carnosus directed towards the trunk muscles or on the surface of trunk muscles rounded and oval mast cells occurred singly or in groups from two to several dozen cells. These groups had no association with blood vessels or hair follicles. Mast cell groups were scarse in 1-month-old, clearly recognizable in 2-months-old and conspicuous in 4-months-old mice of both sexes. The number of mast cells and their number per group was larger in CFW/Ll and C3H than in Balb/c mice. Accumulation of mast cells in subcutaneous connective tissue was noted in animals from two separate breeding centers. The animals were free of ectoparasites and dermatophytes but contained some pinworms and protozoa. Elimination of these parasites, change of diet and drinking water as well as cage lining did not prevent the appearance of mast cell accumulations. These accumulations occurred in all studied mice (over 100) at the age of 2 months or older, and were also found in 1 out of 6 four-month-old hamsters and in 2 out of 6 four-month-old rats. It is suggested that mast cells accumulate in subcutaneous connective tissue in response to some undefined noxious agent. Whatever the reason of their presence, large groups of mast cells could considerably influence the results of tests performed in the skin-hypodermis area.

Animals↗
PubMed1987

Natural killer cell activity of peripheral blood mononuclear cells from patients with various forms of systemic scleroderma.

Peripheral blood mononuclear cells from 63 patients with systemic scleroderma, including incipient or prodromal acrosclerosis, and from 20 healthy individuals were tested for natural killer (NK) cell activity and antibody-dependent cell cytotoxicity in a 4 h 51Cr release assay using K562 and L1210 cell lines respectively. In patients with systemic scleroderma natural killer cell activity was significantly decreased compared with the controls. NK cell activity was markedly lowered in patients with diffuse scleroderma and in transitional form acrosclerosis-diffuse scleroderma, and was normal in cases of acrosclerosis and/or CREST syndrome and in cases of prodromal or incipient scleroderma. Antibody-dependent cell cytotoxicity of mononuclear cells from the systemic scleroderma patients was within the normal range. The lowered natural killer cell activity correlated with the severity of systemic scleroderma, in terms of the extent of skin and organ involvement.

Adult↗
PubMed1987

Natural killer cell activity of peripheral blood mononuclear cells from patients with various forms of lupus erythematosus.

Natural killer (NK) cell activity of peripheral blood mononuclear cells from 28 patients with cutaneous discoid lupus erythematosus, seven with subacute cutaneous lupus erythematosus, and 17 with systemic lupus erythematosus was studied using a 4 h 51Cr release assay using K-562 cells as target cells. NK cell activity was found to be markedly decreased not only in patients with systemic lupus erythematosus but also in a proportion of cases of subacute cutaneous lupus erythematosus and cutaneous discoid lupus erythematosus (57% and 39% respectively). There was a relationship between the activity and severity of LE and the decrease in NK cell activity.

Adult↗
PubMed1987

Serum samples from patients with active psoriasis enhance lymphocyte-induced angiogenesis and modulate endothelial cell proliferation.

In former studies we have shown the increased angiogenic capability of peripheral blood mononuclear cells (MNCs) from patients with the active guttate type of psoriasis vulgaris. The present study tested the effects of serum factors from 67 patients with various forms of psoriasis vulgaris and from 32 healthy individuals on the angiogenic capability of normal human MNCs in an animal system. The angiogenesis-enhancing effect was most marked in the serum samples from patients with plaquelike and peripherally spreading lesions lasting for one to two months, and tended to disappear in patients with long-lasting and/or stationary lesions. Serum samples from patients with psoriasis were also capable of modulating the proliferation of normal human endothelial cells in vitro, and this modulation was correlated with the duration of relapse. The effects of psoriatic sera on normal human MNC function and on endothelial cell growth may be of importance for vascular proliferation in psoriasis.

Cell Division↗
PubMed1986

Defective natural-killer- and killer-cell activity associated with increased polymorphonuclear leukocyte adherence in psoriasis.

Patients (n = 45) with psoriasis vulgaris were studied for natural killer (NK) cell activity and antibody-dependent cell cytotoxicity (ADCC) of their peripheral blood mononuclear cells against K-562 and L1210 target cells, respectively. Simultaneously, the studies were performed on the adherence to human endothelium of polymorphonuclear leukocytes (PMN) from various forms of the disease. Mean values of NK-cell activity in psoriasis vulgaris, as a whole, and mean values of ADCC in male patients were significantly lower than in a group of healthy controls. This decrease was most pronounced in patients with the active form of the disease, in whom in vitro PMN adherence to human endothelium was found to be increased. Neutrophils from both patients with active psoriasis and healthy individuals were capable of reducing NK-cell activity of normal human mononuclear cells.

Adolescent↗
PubMed1986

Partial defects of cell-mediated immunity in patients with epidermodysplasia verruciformis.

Different parameters of cell-mediated immunity, including natural cytotoxic reactions, were studied in nine patients with epidermodysplasia verruciformis with or without cutaneous malignancy. We found decreased total number of T lymphocytes and T-helper cells in peripheral blood of the patients, and normal T-suppressor cell number, as detected by monoclonal antibody typing and functional E-rosette test with the use of theophylline. This decrease was found both in active and in late rosette-forming cell subpopulations. Natural killer cell activity of peripheral blood mononuclear cells was found to be increased in four of nine patients with epidermodysplasia verruciformis, whereas antibody-dependent cellular cytotoxicity was within the normal range in all patients studied. Lymphocyte-induced angiogenesis assay, which is a sensitive test for the estimation of the immunocompetence of lymphoid cells, revealed increased angiogenic capability of peripheral blood mononuclear cells in the majority of the patients. Our results suggest that cellular defects in patients with epidermodysplasia verruciformis did not relate to all functions of the immune system.

Adult↗
PubMed1986

Enhanced angiogenic capability of monocyte-enriched mononuclear cell suspensions from patients with systemic scleroderma.

Different subsets of peripheral blood mononuclear cells (MNC) from 15 patients with systemic scleroderma were tested for their ability to evoke angiogenesis in a xenogenic system. The angiogenic capability of total MNC from patients with systemic scleroderma was lower than that of normal human cells, irrespective of the form of the disease. However, the capability of a monocyte-enriched subset of MNC from patients with scleroderma was found to be increased, as compared with their total MNC and with that of the corresponding subset from healthy individuals. This might be due to the activation of monocytes in the disease.

Adult↗
PubMed1985

Increased natural killer cell activity in patients with epidermodysplasia verruciformis.

Six patients with epidermodysplasia verruciformis (EV) were studied for natural killer cell (NKC) cytotoxic response of their peripheral blood mononuclear cells against K-562 target cells in an 18-hour chromium 51-release assay. Four patients displayed higher cytotoxic responses than controls did, whereas two others did not differ from controls. Patients with EV who had increased NKC activity were found to be infected with potentially oncogenic human papillomaviruses (types 5, 8, 9, 14, 17, 19, 22, 24, and others), and they have had multiple skin carcinomas and/or Bowen's precancerous dermatosis. Two patients with EV who had normal cytotoxic response were free of skin malignancies.

Adult↗