STUDIES ON THE MECHANISM OF POTASSIUM-STIMULATED RESPIRATION IN ESCHERICHIA COLI.
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Biomedical subjects
Publications and source records attributed to S MILLER.
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Experiments were conducted to determine the effects of storage temperatures, relative humidity, and additives on the survival of aerosolized Escherichia coli phage T-3. The aerosol stability of the coliphage, calculated as per cent recovery, was not affected by storage at 10 or -70 C for up to 4 months. However, an increase in aerosol decay rate of coliphage stored at 10 C was observed. The effect of humidities ranging from 20 to 90% relative humidity was studied, and it was observed that humidities lower than 70% relative humidity significantly reduce the survival of airborne coliphage. The effect of various compounds on the aerosol decay rate of T-3 coliphage was studied at 50 and 85% relative humidity. Addition of dextrose in 0.1 M concentrations to the disseminating fluid significantly reduced aerosol decay rate at 50% relative humidity without affecting the decay at 85%. Addition of spermine, spermidine-phosphate, thiourea, galacturonic acid, and glucosaminic acid, individually or in combination, had no effect on aerosol decay rates. The use of deuterium oxide as the suspending fluid for dissemination had no effect on aerosol stability of the coliphage.
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In mud houses in Wakara, Upper Volta, dichlorvos dispensers installed at the rate of one dispenser per approximately 500 cubic feet (14 m(3)) produced 70% or higher average kills of adult Aëdes aegypti, exposed overnight in screen wire cages suspended at levels of 6 feet (1.8 m) and 2 feet (0.6 m), for approximately three months during the wet season and four months during the dry season. Average kills of 96% and 80% were obtained in the dry season at the 6-foot and 2-foot levels, respectively, with 6-hour daytime exposures 17-22 weeks after treatment.
The residual fumigant technique involving the use of dichlorvos in the solid dispenser formulation employed in the study reported in this paper appears to offer no hazard to the occupants of houses treated at the rate of one dispenser per 500 cubic feet (14 m(3)) in so far as dichlorvos vapour toxicity is concerned. Even the maximum dosage rate tested (one dispenser per 60 cubic feet (1.7 m(3)) or 28 dispensers in a two-room house) did not produce observable effects on the occupants of the houses. This maximum rate far exceeds that necessary for malaria eradication operations, thereby affording an additional safety factor.
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