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S M Wolf

Publications and source records attributed to S M Wolf.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

Application of capillary liquid chromatography coupled with tandem mass spectrometric methods to the rapid screening of adducts formed by the reaction of N-acetoxy-N-acetyl-2-aminofluorene with calf thymus DNA.

Capillary liquid chromatography-continuous-flow fast atom bombardment mass spectrometry is applied to the detection of deoxynucleoside adducts of N-acetoxy-N-acetyl-2-aminofluorene. In such a configuration, normal scan and tandem mass spectrometric techniques are shown to provide useful structural information for an N-acetyl-N-(deoxyguanosin-8-yl)-2-aminofluorene adduct standard for low- nanogram (low-picomole) amounts sampled. In addition, multiple reaction monitoring gives limits of detection below 25 pg (50 fmol) for this adduct, suggesting the potential for routine screening of intact deoxynucleoside adducts formed below the 1:10(6) level from as little as 1 mg of DNA. When applied to the analysis of the products of an in vitro reaction of calf thymus DNA with N-acetoxy-N-acetyl-2-aminofluorene, these techniques are readily able to detect and supply structural data for the N2 and C8 deoxyguanosine adducts formed.

Acetoxyacetylaminofluorene↗
PubMedDate not supplied

Methohexital: a practical review for outpatient dental anesthesia.

Methohexital is an ultrashort-acting barbiturate widely used in dentistry because of its rapid onset, predictable effects, and short duration of action. Like other barbiturates, methohexital exerts its effects through the gamma-aminobutyric acid (GABA) receptor complex. By binding to its own receptor on the complex, methohexital augments the inhibitory effect of GABA on neurons and additionally can exert a similar effect independent of GABA. After intravenous injection, maximal brain concentrations are achieved within 30 sec and then quickly fall as the drug is redistributed to other tissues, yielding a duration of action after a single dose of 4 to 7 min. Hepatic metabolism accounts for elimination of the drug. Methohexital at conventional doses in healthy individuals is a mild respiratory depressant with modest cardiovascular effects. Adverse effects, however, can include apnea, cardiovascular depression, laryngospasm, hiccough, and allergic-like reactions. Although more recently introduced drugs, such as midazolam, etomidate, and propofol, have specific advantages, methohexital remains a drug of choice for dental outpatient anesthesia because of its low cost, rapid onset, short duration, lack of secretory or emetic properties, and proven history.

Anesthesia, Dental↗
PubMedDate not supplied

Doing ethics in Italy.

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Bioethical Issues↗