Search PubMed⌕ Search

Biomedical subjects

S M Shah

Publications and source records attributed to S M Shah.

At least 37 records · Page 2Linked to original sources
PubMed1999

Ocular complications of intravenous cidofovir for cytomegalovirus retinitis in patients with AIDS.

PURPOSE: To describe the frequency of anterior uveitis and ocular hypotony in cidofovir-treated patients with acquired immune deficiency syndrome (AIDS)-related cytomegalovirus (CMV) retinitis. METHODS: A retrospective review was performed of all patients with AIDS-related CMV retinitis during a 12-month period. The CMV retinitis activity, concurrent illnesses and medications, and CD4+ lymphocyte count were recorded in addition to the degree of anterior chamber inflammation and intraocular pressure at each visit. The frequency of uveitis and ocular hypotony in cidofovir-treated patients was determined and the possible influence of other ocular and systemic factors considered. RESULTS: Eight of 9 patients on cidofovir developed anterior uveitis. The cellular anterior chamber activity resolved with topical corticosteroid administration in all eyes with uveitis but significant flare persisted despite topical steroids in 3 patients. Posterior synechiae responded poorly to topical mydriatic therapy, resulting in inadequate mydriasis which significantly limited the fundal view. One patient developed a visually significant unilateral hypotonous maculopathy. CONCLUSIONS: Patients treated with intravenous cidofovir for AIDS-related CMV retinitis are at significant risk of ocular adverse effects. Prompt treatment with topical corticosteroids and mydriatics may control uveitis and in some cases cidofovir treatment may be cautiously continued. In the event of ocular hypotony cidofovir should be discontinued in favour of an alternative anti-cytomegaloviral agent.

AIDS-Related Opportunistic Infections↗
PubMed1998

Measurement of electrostatic charge on intraocular lenses.

PURPOSE: An electronic meter that measures electrostatic charge was designed and built. The hypothesis tested was that an intraocular lens (IOL) attains a charge during handling in the operating theatre and that washing the IOL with balanced salt solution (BSS) would result in a reduction in the surface charge. Once exposed to the air a charged IOL may attract particulate matter to its surface before implantation into the eye. METHODS: This experiment was performed in the operating theatre under sterile conditions mimicking cataract surgery. The lens box was opened onto a trolley and the lens container opened by a scrubbed assistant. The operator (also scrubbed) removed the lens with MacPherson's forceps. The charge on the lens was then measured without delay, or after the lens had been washed with 1-2 ml of BSS. RESULTS: The mean charge on unwashed lenses was 1.43 x 10(-10) C (n = 12) and on washed lenses was 0.59 x 10(-10) C (n = 10). The difference in the charges was significant (p = 0.03, unpaired t-test). CONCLUSION: Washing of an IOL prior to implantation will reduce the surface charge and is therefore also likely to reduce the chance of contamination.

Cataract Extraction↗
PubMed1997

The spectrum of eye disease in children with AIDS due to vertically transmitted HIV disease: clinical findings, virology and recommendations for surveillance.

BACKGROUND: Eye disease in children infected with HIV is uncommon, but there is little information on which children require screening or surveillance and the management of those with retinitis. METHODS: We examined 12 children with symptomatic HIV disease (of a cohort of 98 HIV-positive children) using indirect ophthalmoscopy. When retinitis was found it was documented photographically at the time of diagnosis and on follow-up. RESULTS: Four cases of infective retinitis were diagnosed: three with probable cytomegalovirus retinitis and one with progressive outer retinal necrosis. CONCLUSION: Detection of reactivation is difficult and we recommend fundus photography under general anaesthesia wherever possible. Children with other end-organ disease, symptomatic eye disease and advanced HIV disease with severe immunosuppression require ophthalmological surveillance.

Acquired Immunodeficiency Syndrome↗
PubMed1997

Effect of basic fibroblast growth factor on myocardial angiogenesis in dogs with mature collateral vessels.

OBJECTIVES: We sought to evaluate the potential of basic fibroblast growth factor (bFGF) to enhance coronary collateral perfusion in dogs with chronic single-vessel coronary occlusion. A secondary goal was to examine whether the salutary effects of bFGF treatment, previously proved effective in the short term, would be maintained in the long term (6 months). BACKGROUND: bFGF, an angiogenic growth factor, is currently the subject of a Phase I trial in patients with ischemic heart disease. It has been shown to promote collateral development in dogs with progressive coronary occlusion when given during the period of natural collateralization. The effect of bFGF on quiescent collateral vessels, a subject of significant clinical importance, is uncertain. METHODS: Dogs were subjected to ameroid-induced occlusion of the left circumflex coronary artery and randomized to bFGF (1.74 mg/day for 7 days), a regimen previously proved effective, or to saline solution. Maximal collateral perfusion was assessed 6 months later, and the dogs were reassigned to a course of bFGF or saline solution. Collateral perfusion was reevaluated after the second treatment course. RESULTS: At 6 months, collateral function was identical in the groups treated initially with bFGF and saline solution. The subsequent course of bFGF did not induce further collateralization. CONCLUSIONS: Although we previously demonstrated the salutary effects of this bFGF regimen in the short term (5 weeks), collateral flow in control dogs reached parity with that of bFGF-treated dogs after 6 months. bFGF did not induce further collateralization in dogs with mature collateral vessels, underscoring the priming role of ischemia for bFGF-induced collateral development.

Animals↗
PubMed1997

Two-plane modified radical retropubic prostatectomy.

Salvage radical prostatectomy may be effective in patients refractory to radiation therapy. We describe a modified technique of radical retropubic prostatectomy, whereby a transverse incision in the cul-de-sac is made and the seminal vesicles and vas deferens are dissected away from the posterior surface of the bladder. This plane which follows the anterior layer of Denonvilliers' fascia is carried up to the bladder neck. The second plane of dissection follows the posterior layer of Denonvilliers' fascia up to the apex of the prostate. The peritoneal incision in the cul-de-sac is closed. After entering the space of Retzius the endopelvic fascia is incised and the remainder of the procedure follows the standard retropubic approach. This technique may be used for salvage prostatectomy to avoid rectal injury.

Humans↗
PubMed1997

Unusual presentation of a pancreatic pseudocyst. A case report and review of literature.

RESULTS AND CONCLUSIONS: Pancreatic pseudocysts may simulate renal cysts symptomatically and radiographically. BACKGROUND: Pancreatic disease can often mimic renal disease. This is especially true of pancreatic pseudocysts. METHODS: We report an interesting case of a pancreatic pseudocyst mimicking a renal cyst on a computed tomography (CT) scan. The clinical anatomy of retroperitoneal spaces and pathways followed by pancreatic secretions is examined. The literature on pancreatic diseases simulating renal disorders is reviewed.

Humans↗
PubMed1997

Surgically correcting a vesicouterine fistula with a myouterine flap. A case report.

BACKGROUND: The incidence of vesicouterine fistula has been increasing, most probably secondary to a corresponding increase in the use of low segment cesarean section. CASE: A 37-year-old woman with a history of two cesarean sections, 14 years and 5 months earlier, presented with urge incontinence, cyclic hematuria and amenorrhea. Hysterosalpingography demonstrated contrast with the bladder and suggested a vesicouterine fistula. Following exploratory laparotomy and dissection of the bladder from the uterus, a fistula was seen connecting the anterior surface of the uterus and the posterosuperior aspect of the bladder. The fistula, with a cuff of uterus and bladder, was excised and the remaining defects repaired. In addition, a myouterine flap was raised to reinforce the repair. Upon follow-up the patient reported no difficulty in urination, complete urinary continence, normal menses and no hematuria. CONCLUSION: This is the first case of vesicouterine fistula repaired with a myouterine flap. This technique strengthens the repair and is especially convenient due to its easy accessibility. A myouterine flap can be utilized if the omentum is of insufficient length or absent. The risk of postoperative bowel obstruction may be decreased as compared to omental interposition.

Adult↗
PubMed1996

Detection of HIV-1 DNA in needle/syringes, paraphernalia, and washes from shooting galleries in Miami: a preliminary laboratory report.

Shared use of injection equipment (needle/syringes), registering, booting, and backloading are practices among injection drug users (IDUs) that increase the risk for transmission of human immunodeficiency virus type 1 (HIV-1). The sharing of injection paraphernalia (including cookers and cottons) and washwater for rinsing used needle/syringes and dissolving drugs could be potential sources for secondary transmission of HIV-1. Laboratory rinses were made from needle/syringes, cottons, and cookers obtained from shooting galleries, and washwaters were obtained from shooting galleries in Miami. Three rinses were analyzed and antibodies to HIV-1 proteins were detected by using Western blot and HIV-1 DNA was detected by using nested polymerase chain reaction (PCR) specific for the gag and envelope genes of HIV-1. Antibodies to HIV-1 proteins were detected in 12 (52%) of 23 rinses from visibly contaminated needle/syringes, in three (18%) of 17 rinses from cottons, in three (14%) of 21 rinses from cookers, and in one (6%) of 17 washwaters. No antibodies were detected in laboratory rinses from visibly clean needles. Using nested PCR followed by Southern blot confirmation of the amplified targets, HIV-1 gag gene DNA was detected in 16 (84%) of 19 and envelope gene DNA in 17 (85%) of 20 laboratory rinses from visibly contaminated needle/syringes. We detected gag and envelope gene DNA, respectively, in three (27%) and four (36%) of 11 cottons, in six (46%) and seven (54%) of 13 cookers, and in five (38%) of 13 and in 10 (67%) of 15 washwaters from shooting galleries. No HIV-1 DNA was detected in laboratory rinses from visibly clean needles. These results indicate that HIV-1 might be present in contaminated cottons, cookers, and washwaters as well as in contaminated needle/syringes at shooting galleries. Reduction of risks of exposure to HIV-1 among IDUs may require modification of behaviors that are ancillary to the act of injection, such as the use of common cookers, cottons, and washwater.

Blotting, Southern↗
PubMed1995

Correlations between aqueous flare and cells and lens surface cytology in eyes with poly(methyl methacrylate) and heparin-surface-modified intraocular lenses.

A prospective, single-surgeon study was performed on 59 patients randomized to receive a poly(methyl methacrylate) intraocular lens or a heparin-surface-modified lens after standardized endocapsular cataract surgery with a routine postoperative regimen. The anterior chamber laser flare and cell measurements and the cellular reaction on the anterior lens surface were monitored for a year following surgery. This report examines the correlations between the anterior chamber flare and cell reaction and the anterior IOL surface cellular reaction. The findings suggest that the early postoperative blood-aqueous barrier breakdown is primarily caused by surgical trauma; by one month the blood-aqueous barrier function is determined by inflammatory mediators released by small monocyte-derived cells on the IOL surface. Giant cells on the IOL surface did not correlate with aqueous flare, which suggests an insignificant capacity to release inflammatory mediators.

Aqueous Humor↗
PubMed1995

Comparison of the postoperative inflammatory response in the normal eye with heparin-surface-modified and poly(methyl methacrylate) intraocular lenses.

Evidence that poly(methyl methacrylate) (PMMA) is not inert in the eye has led to the introduction of intraocular lens (IOL) surface modification. In this prospective, double-blind, randomized study, we evaluated the effects of heparin surface modification (HSM) on anterior segment inflammation for one year after endocapsular cataract surgery. Fifty-four eyes were randomized to receive an HSM IOL (29 eyes) or a PMMA IOL (25 eyes) and were assessed postoperatively by corneal endothelial photography, laser flare and cell measurements, fluorophotometry, and IOL surface specular microscopy. Corneal endothelial loss, mean aqueous flare and cells, and fluorophotometry did not differ significantly between the groups, although fewer eyes in the HSM group had high flare values on the first postoperative day. The number of giant cells was significantly less in the HSM group for up to one year after surgery. Although there was no statistical difference in overall visual outcome, more patients in the HSM group (17%, 5/29) achieved a postoperative visual acuity of 20/15 than in the PMMA group (0). These results demonstrate that HSM enhances IOL biocompatibility and these effects are detectable in the low-risk, normal eye.

Aged↗
PubMed1995

Natural history of cellular deposits on the anterior intraocular lens surface.

Previous cytopathological studies have demonstrated the presence of cellular deposits on the poly(methyl methacrylate) (PMMA) intraocular lens (IOL) surface. In this prospective study, IOL surface specular microscopy was used to document the natural history of these deposits in the first year following PMMA IOL implantation. Intraocular lens surface specular microscopy was performed 1, 3, 6, and 12 months after endocapsular cataract surgery in 27 otherwise normal eyes. Postoperatively, IOLs were assessed for the presence of inflammatory cells, with the number of cells graded from 0 (none) to 4 (many). Two inflammatory cell types were visualized: small and giant cells. Small cells were found on 81.5% of IOLs at 1 month, 73.1% at 3 months, and 30.4% at 12 months. Small cells were found in only small numbers (peak mean cell score of 1.26 +/- 0.94 at 1 month). Giant cells were found on 59.3% of lenses at 1 month, 73.1% at 3 months, and 39.1% at 12 months. The number of giant cells on each lens peaked at 3 months (mean cell score 2.0 +/- 1.55), when 50% of lenses achieved a giant cell score of 3 or 4. Inflammatory cell deposits are, therefore, a normal occurrence on the PMMA IOL surface for up to 1 year after surgery. This cellular response consists of two distinct processes: a small cell response, which peaks by 1 month, and a later giant cell response, which peaks at 3 months.

Aged↗