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Biomedical subjects

S M Kim

Publications and source records attributed to S M Kim.

At least 55 records · Page 3Linked to original sources
PubMed2005

Observation of B0-->D*sJ(2317)+K- decay.

The decays B0-->D+sJK- and B0-->D-sJpi+ are studied for the first time. A significant signal is observed in the B0-->D*sJ(2317)+K- decay channel with B(B0-->D*sJ(2317)+K-) x B(D*sJ(2317)+-->D+spi0)=(5.3(+1.5)(-1.3)+/-0.7+/-1.4) x 10(-5). No signals are observed in the B0-->D*sJ(2317)-pi+, B0-->DsJ(2460)+K-, and B 0-->DsJ(2460)-pi+ decay modes, and upper limits are obtained. The analysis is based on a data set of 140 fb(-1) collected by the Belle experiment at the asymmetric e+e- collider KEKB.

Journal Article↗
PubMed2005

An acute inflammatory response alters bone homeostasis, body composition, and the humoral immune response of broiler chickens.

To quantify the effects of an acute phase response in broilers, chicks were injected with 1 mg/kg Escherichia coli lipopolysaccharide (LPS) at 15 and 23 d. Lipopolysaccharide injection increased feed/gain (P = 0.03), increased liver weight (P = 0.09), and decreased tibia calcium (P = 0.05) and breaking strength (P < 0.04) by d 28. In a second experiment, 3 d postinjection of chicks at d 31, LPS decreased BW (P < 0.01), breast weight (P = 0.08), and tibia breaking strength (P = 0.05), and increased liver weight (P < 0.01), mortality (P = 0.05), and titers to bronchitis and Mycoplasma gallisepticum that were induced by vaccination at hatch or by field exposure, respectively (P = 0.04). For experiment 3, chicks were challenged with LPS at 23d and 27d. Lipopolysaccharide-injected chicks had decreased BW (P = 0.06), feed consumption (P = 0.05), tibia weight (P< 0.01), and breaking strength (P < 0.01), and increased feed/gain (P < 0.01), liver weight (P < 0.01), and plasma ionized calcium level (P = 0.08). For experiment 4, chicks were injected with 0, 0.33, 0.66, 1.00, or 4.25 mg of LPS/kg of BW. There was an inverse relationship between LPS level and BW or bone breaking strength. Experiment 5 compared 4 broiler strains. Strain x LPS interactions were found for bone breaking strength (P = 0.01). Mortality before LPS challenge was inversely correlated to liver weight (r2 = 0.95, P = 0.02) and bone breaking strength (r2 = 0.99, P = 0.01) only after an LPS challenge.

Acute-Phase Reaction↗
PubMed2005

Serum osteoprotegerin levels are associated with inflammation and pulse wave velocity.

OBJECTIVE: We examined the association between serum osteoprotegerin (OPG) levels, systemic inflammation and arterial stiffness in normal and diabetic patients. PATIENTS AND MEASUREMENTS: The study subjects comprised 49 newly diagnosed diabetic patients and 72 age- and sex-matched normal glucose controls. Anthropometric parameters, blood pressure, fasting blood glucose (FBG), lipid profiles, serum OPG, high-sensitive C-reactive protein (hsCRP), interleukin-6 (IL-6) and brachial-ankle pulse wave velocity (baPWV) were measured. RESULTS: Serum OPG levels (6.1 +/- 1.4 vs. 5.4 +/- 1.3 pmol/l, P = 0.011) and baPWV (1562 +/- 354 vs. 1399 +/- 257 cm/s, P = 0.004) were significantly higher in the diabetic group than in the normal glucose group. Serum OPG levels in normal and diabetic patients correlated significantly with systolic blood pressure (r = 0.20, P = 0.035), FBG (r = 0.30, P = 0.002), right baPWV (r = 0.22, P = 0.021), left baPWV (r = 0.26, P = 0.006), homeostasis model assessment insulin resistance (HOMA-IR) (r = 0.19, P = 0.045), IL-6 (r = 0.32, P = 0.001) and hsCRP (r = 0.21, P = 0.027) after adjusting for age and sex. Multiple regression analysis showed that serum OPG level was significantly associated with age, FBG, IL-6, systolic blood pressure, triglyceride and hsCRP (R(2) = 0.299). CONCLUSIONS: In summary, serum OPG and baPWV levels are elevated in diabetic patients and serum OPG levels are significantly associated with inflammation and arterial stiffness.

Biomarkers↗
PubMed2005

Accuracy of finite element model-based multi-organ deformable image registration.

As more pretreatment imaging becomes integrated into the treatment planning process and full three-dimensional image-guidance becomes part of the treatment delivery the need for a deformable image registration technique becomes more apparent. A novel finite element model-based multiorgan deformable image registration method, MORFEUS, has been developed. The basis of this method is twofold: first, individual organ deformation can be accurately modeled by deforming the surface of the organ at one instance into the surface of the organ at another instance and assigning the material properties that allow the internal structures to be accurately deformed into the secondary position and second, multi-organ deformable alignment can be achieved by explicitly defining the deformation of a subset of organs and assigning surface interfaces between organs. The feasibility and accuracy of the method was tested on MR thoracic and abdominal images of healthy volunteers at inhale and exhale. For the thoracic cases, the lungs and external surface were explicitly deformed and the breasts were implicitly deformed based on its relation to the lung and external surface. For the abdominal cases, the liver, spleen, and external surface were explicitly deformed and the stomach and kidneys were implicitly deformed. The average accuracy (average absolute error) of the lung and liver deformation, determined by tracking visible bifurcations, was 0.19 (s.d.: 0.09), 0.28 (s.d.: 0.12) and 0.17 (s.d.: 0.07) cm, in the LR, AP, and IS directions, respectively. The average accuracy of implicitly deformed organs was 0.11 (s.d.: 0.11), 0.13 (s.d.: 0.12), and 0.08 (s.d.: 0.09) cm, in the LR, AP, and IS directions, respectively. The average vector magnitude of the accuracy was 0.44 (s.d.: 0.20) cm for the lung and liver deformation and 0.24 (s.d.: 0.18) cm for the implicitly deformed organs. The two main processes, explicit deformation of the selected organs and finite element analysis calculations, require less than 120 and 495 s, respectively. This platform can facilitate the integration of deformable image registration into online image guidance procedures, dose calculations, and tissue response monitoring as well as performing multi-modality image registration for purposes of treatment planning.

Algorithms↗
PubMed2005

The role of ERK1/2 activation in the infection of HeLa cells with Human coxsackievirus B3.

Human coxsackievirus B3 (CVB3) is known to trigger in host cells a biphasic activation of extracellular signal-regulated kinase (ERK1/2); i.e., early transient and late sustained activation. In this study, we explored (i) the role of ERK1/2 activation in virus entry into cells and virus replication and (ii) cellular genes influenced by this activation in CVB3-infected HeLa cells. Pretreatment of the cells with an ERK1/2 inhibitor, PD98059 showed that early transient ERK1/2 activation is not be related to virus entry, but late sustained ERK1/2 activation plays a role in virus replication. To identify which cellular genes are influenced by the ERK1/2 activation after virus infection, a cDNA microarray analysis was performed. In HeLa cells pretreated with PD98059 and then infected with the virus, the number of influenced cellular genes was higher compared to that in infected cells not pretreated with the inhibitor (15 vs 77 at 10 mins post infection (p.i.) and 347 vs 91 at 9 hrs p.i. Thus the virus infection affected several host genes through ERK1/2 activation.

Enterovirus B, Human↗
PubMed2004

Expression of FasL and perforin/granzyme B mRNA in chronic hepatitis B virus infection.

Cytotoxic T lymphocytes are essential components of immune responses during chronic hepatitis B (CHB). It has been known that Fas ligand (FasL) and perforin/granzyme B-based mechanisms account for all T cell-mediated cytotoxicity. In the present work, we examined the correlation between injury of the hepatocytes and mRNA expression of FasL and perforin/granzyme B in liver tissue to investigate the roles of both the FasL and the perforin/granzyme B pathways in CHB. Reverse transcription-polymerase chain reaction was used to identify intrahepatic expression of FasL and perforin/granzyme B in liver biopsy specimens from 24 patients with hepatitis B virus (HBV) infection. In addition, the transferase-mediated deoxyuridine triphosphate nick end-labelling (TUNEL) method was used to determine the degree of apoptosis. The degree of mRNA expression and apoptosis were compared with the histologic activity index (HAI) and serology, including alanine aminotransferase (ALT). Intrahepatic mRNA expression rates of FasL, perforin and granzyme B were seen in 79.2, 62.5 and 33.3% of patients, respectively, and correlated with ALT levels (P < 0.05). Intrahepatic expression of FasL and perforin mRNA were significantly correlated with HAI (P < 0.05). Also, apoptosis documented by the TUNEL assay was correlated with HAI and intrahepatic mRNA expression of FasL and perforin (P < 0.05). Our results show that the T-cell mediated perforin death pathway as well as the Fas system play important roles in liver cell injury in HBV infection and that apoptosis mediated by the Fas/FasL system is closely correlated with HAI in chronic HBV infection.

Alanine Transaminase↗
PubMed2004

Concomitant reconstruction of mandibular basal and alveolar bone with a free fibular flap.

Repair of long-span mandibular defects with a free fibular flap is now a routine procedure. However, the bone height of the neo-mandible after reconstruction with a fibular flap is about half that of the dentulous mandible. When a fibular graft is placed only at the inferior border of the mandible, the resulting vertical discrepancy between the graft segment and the occlusal plane can adversely affect implant mechanics or denture stability and retention. To overcome these problems, we developed a technique for two-strut type mandibular reconstruction. A vascularized fibular segment is used to reconstruct the inferior basal portion of the neo-mandible, while a non-vascularized residual fibular segment is used to simulate the superior alveolar portion. We used this technique in 22 patients. Graft survival, graft resorption, and the ability to place implants were assessed as compared with those after the conventional one-strut type technique. The fibular segment grafted to the alveolar region was removed in one patient with intraoral wound dehiscence and in two with postoperative infection. All vascularized fibular flaps were successful. The resorption rate was 13.6+/-7.2% for non-vascularized segments and 3.0+/-3.7% for vascularized segments. Dental implants were placed in five of our 22 patients. The crown:fixture length ratio was improved to 1:1.7, as compared with a ratio of 1:1.21 with use of a conventional fibular flap. We conclude that our technique is very easy and safe and provides substantially improved lower-lip and cheek support and implant-prosthetic mechanics than conventional procedures for the repair of long-span mandibular defects.

Adult↗
PubMed2004

Preferential expression of L-type amino acid transporter 1 in ameloblasts during rat tooth development.

Certain amino acid transport systems play an important role in supplying organic nutrients to each cell and for cell proliferation during tooth development. However, the mechanisms responsible for such actions are unclear. This study demonstrated for the first time that LAT1 and 4F2hc are expressed during tooth development in prenatal and postnatal rats, and that the transporters show cell-specific expression in ameloblasts, which are the epithelium-derived dental cells. LAT1 and 4F2hc expression was not observed in other dental cells of the developing teeth such as odontoblasts and cementoblasts. Overall, these results suggest that LAT1 and 4F2hc might play an important role in enamel formation.

Ameloblasts↗
PubMed2004

Influence of mass transfer on the ozonation of wastewater from the glass fiber industry.

The mass transfer rate (kLa) is one of the most important parameters in the ozonation of wastewater, because it frequently constitutes the rate-determining step. This study investigated the influence of kLa on the ozonation of glass fiber wastewater using a high-performance jet loop reactor (HJLR), which is well known for its high mass transfer property, and compared the results of this investigation with those obtained using the bubble column reactor. It was found that the higher kLa achieved by increasing the energy input did not lead to higher ozonation efficiency, since the reaction involving the OH radical was greatly hindered at the low pH produced as a result of ozonation. By maintaining the pH at a value greater than 8.0, the higher kLa in the HJLR reactor contributed to increasing not only the TOC removal of wastewater, but also the ozone consumption efficiency, as expressed by the specific ozone consumption. The specific ozone consumption in the HJLR reactor (7.1 g ozone/ g TOC) was 20% better than that in the bubble column reactor.

Glass↗
PubMed2004

Enhanced aerobic floc-like granulation and nitrogen removal in a sequencing batch reactor by selection of settling velocity.

In order to enhance the solid-liquid separation properties and nitrogen removal efficiency of SBR, the aerobic floc-like granules were cultivated under temporal alternating aerobic and anoxic conditions without the presence of carrier material in a SBR having 15 H/D (height/diameter) ratio. Two different effluent port positions were applied to the SBR for different selection of minimum settling velocities (over 0.6 and 0.7 m/h) of granules retained in the SBR during aerobic floc-like granule formation. The effect of different minimum settling velocities as an operational parameter on the size and solid-liquid separation properties of floc-like granules and also the COD and nitrogen removal of SBR were evaluated. The reactor was operated 6 hours per cycle (aerobic 4.75 hours, anoxic 1.25 hours) under chemical oxygen demand (COD) loading rate of 2.5 kg/m3 x d (1.3 kg acetate-COD and 1.2 kg glucose-COD). When increasing the minimum settling velocity by 0.1 m/h, the following results were observed at steady state. The nitrification efficiency was not changed at about 97% but the denitrification efficiency was improved from 78 to 97%. The COD removal efficiency was improved from 82 to 97% and the concentration of biomass in the reactor was retained at lower level at about 3,000 mg MLSS/L. The average sludge volume index (SVI) value of granules was decreased about 85 to 50 mL/g and the granule sizes were increased 0.1-0.5 mm to 1.0-2.0 mm. The required time to form granules and reach steady state was significantly shortened. Based on the results, the selection of the minimum settling velocity had a significant effect on both the physical properties of granules and the SBR performance, so it is suggested to use the minimum settling velocity as an operational parameter.

Acetates↗
PubMed2003

Comparison of general and epidural anesthesia in elective cesarean section for placenta previa totalis: maternal hemodynamics, blood loss and neonatal outcome.

There are few consistent guidelines in choosing anesthesia for cesarean section for a parturient with placenta previa. This prospective randomized trial was organized to compare the maternal hemodynamics, blood loss and neonatal outcome of general versus epidural anesthesia for cesarean section with the diagnosis of grade 4 placenta previa. After giving informed consent, 12 patients received general anesthesia and 13 received epidural. Intraoperative blood pressures demonstrated a more stable course in the epidural group than in the general group. Blood loss did not differ significantly between the groups (1622 +/- 775 mL vs. 1418 +/- 996 mL). General anesthesia resulted in lower immediate postoperative hematocrit level (28.1 +/- 3.5% vs. 32.5 +/- 5.0%, P < 0.05). The patients in the general group received a significantly larger transfusion than the epidural group (1.08 +/- 1.6 vs. 0.38 +/- 0.9 units, P < 0.05). The Apgar scores at 1 and 5 min were similar in the two groups (8 [4-9] vs. 8 [7-9] and 10 [6-10] vs. 9 [9-10], respectively). We concluded that epidural anesthesia is superior to general anesthesia in elective cesarean section for grade 4 placenta previa with regard to maternal hemodynamics and blood loss. There was no difference in neonatal outcome.

Journal Article↗
PubMed2003

Expression of HMGI(Y) associated with malignant phenotype of human gastric tissue.

AIMS: We evaluated the association between HMGI(Y) expression and the detection of malignant cells by simple reverse transcriptase-polymerase chain reaction (RT-PCR) and correlated the level of HMGI(Y) expression and the clinicopathological data in gastric cancer. METHODS AND RESULTS: We analysed HMGI(Y) expression in 62 gastric cancer tissues and 28 normal gastric tissues by RT-PCR and immunohistochemical study. HMGI(Y) expression evidenced by RT-PCR was observed in 42 (67.7%) of 62 gastric cancer samples, whereas eight (28.6%) of 28 normal gastric tissues were positive (P = 0.001). In immunohistochemical staining for HMGI(Y), 48 (77.4%) of 62 gastric cancers were positive for HMGI(Y), whereas four (6.5%) of 62 normal gastric mucosae around the tumour were weakly positive, particularly in cells of some hyperplastic glands (P < 0.001). There was no significant correlation between the levels of HMGI(Y) expression and stage, tumour size, histological grade, invasion depth, or lymph node metastasis. CONCLUSIONS: Our results suggest that HMGI(Y) expression could be associated with malignant phenotype in human gastric tissue, and that HMGI(Y) may contribute to gastric tumorigenesis.

Adenocarcinoma↗
PubMed2002

Imaging features of ischial bursitis with an emphasis on ultrasonography.

OBJECTIVE: The aim of this study was to evaluate the imaging features of ischial bursitis with an emphasis on ultrasonography (US). DESIGN AND PATIENTS: Our study included 31 patients with a painful mass or tenderness in their buttock who underwent US (n=27), CT (n=1), or MR imaging (n=4). A needle aspiration (n=6) or a bursal excision (n=5) was performed in those patients who had no clinical improvement in spite of the conservative treatment. Evaluation included lesion location, size, wall of the bursae, and intrinsic characteristics on US, CT and MR imaging. RESULTS: Ischial bursitis was superficial to the ischial tuberosity in all patients (n=31). The lesion ranged from 1.5 cm to 7 cm (average 3.8 cm) in diameter. The bursal wall was identifiable in 25 cases (81%). Internal septa and mural nodules were seen in 12 (39%) and 17 cases (55%), respectively. Sonography showed that fluid within the bursa was hypoechoic (59%), hyperechoic (26%), or of mixed echogenicity (15%). The bursae were compressible by the transducer. Power Doppler examination (n=7) showed hypervascularity of the bursal wall. All lesions imaged with contrast-enhanced CT and MR imaging had an enhancing thin wall and mural nodule. CONCLUSIONS: Ischial bursitis, superficial to the ischial tuberosity, can be clearly demonstrated on sonography and appears as a thin-walled cystic lesion, with or without internal septa and mural nodules.

Adult↗
PubMed2002

Comparison of effects of angiotensin peptides in the regulation of clitoral cavernosum smooth muscle tone.

The isometric tension measurement and in vitro autoradiography were used in clitoral cavernosum smooth muscle (CSM). Angiotensin ANG III, ANG IV, ANG II and ANG I induced contractions in clitoral CSM strips. ANG III and ANG I- induced contraction was five times less active than ANG II, whereas ANG IV-induced contraction was 1181-fold less potent than ANG II. Contractile responses to ANG III, ANG IV, ANG II and ANG I were significantly inhibited by type 1 ANG II (AT 1) receptor antagonist Dup 753 but not by type 2 ANG II (AT2) receptor antagonist PD 123,319. Pre-treatment with Nomega-nitro-L-arginine methyl ester, nitric oxide (NO) synthase inhibitor accentuated force of contraction induced by ANG III, ANG IV and ANG II. Amastatin, an aminopeptidase inhibitor enhanced ANG III- and ANG IV-induced contractions. Specific binding sites for 125I-ANG II were found in the clitoral CSM. Specific binding of 125I-ANG II was displaced by unlabeled ANG peptides. This study suggests that the contractile responses to all four peptides of the ANG family are mediated via AT1 receptors but not AT2 receptors. Further, the rank order of potency of contraction was as follows, ANG II> ANG I>ANG III>ANG IV. It is also suggested that peptides of the ANG family have a cross-talk with the NO system and aminopeptidase is involved in the modulation of the tone of clitoral CSM by ANG III and ANG IV.

Acetylcholine↗
PubMed2001

Localization of Claude's syndrome.

Although the red nucleus often has been suggested to be the lesion site responsible for Claude's syndrome, the precise localization of the syndrome is uncertain. Lesion sites were reviewed in six patients with Claude's syndrome and compared with other patients with the syndrome identified by a literature search. The findings strongly suggest that Claude's syndrome occurs because of a lesion of the superior cerebellar peduncle just below and medial to the red nucleus. Midbrain infarction and partial oculomotor nerve palsy was common.

Adult↗