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Biomedical subjects

S M Green

Publications and source records attributed to S M Green.

At least 145 records · Page 8Linked to original sources
PubMed1986

A human-human monoclonal anti-D by direct fusion with a lymphoblastoid line.

The human lymphoblastoid cell line W1-L2-729-HF2 has been fused with B cells from a plasmaphoresed anti-D donor immunized with D+ cells. A stable monoclonal antibody-producing cell line has been produced which yields culture supernatant of good titre without the need for concentration. The production and use of this reagent as an alternative Rh D typing reagent for use by saline and enzyme manual tests and automated tests in a Technicon 16C machine is discussed. Du red cells are detected in enzyme enhanced tests.

Antibodies, Anti-Idiotypic↗
PubMed1985

Irreducible dorsal dislocations of the proximal interphalangeal joint.

Dorsal dislocations of the proximal interphalangeal joint are common injuries, and reduction is usually obtained by manipulation. We describe four cases of dorsal dislocation in which attempts at reduction by manipulation were not successful. Three of these injuries were open and one was closed. At surgery, the main block to reduction in all cases was the palmar plate that had ruptured from its proximal attachment and was interposed between the joint surfaces.

Adolescent↗
PubMed1984

Bilateral wrist dislocation in an above knee amputee with advanced rheumatoid arthritis. Attempts at ambulation.

The ambulatory difficulties of a patient with long standing rheumatoid arthritis with an above knee amputation and bilateral wrist dislocations has not been previously reported. Although wrist fusion did in fact increase his hand strength, control and stability, he still lacked sufficient hand strength and grip to don and doff the prosthesis, nor could he use assistive devices in order to become an independent ambulator. The results of wrist fusion and attempts at ambulation are discussed.

Aged↗
PubMed1981

Injection injuries of the hand.

This type of injury is usually the result of an occupational accident, drug abuse, or is iatrogenic in origin. Identification of the injected material, point of entry, and the existence of neurovascular compromise or infection are essential to proper management. Prompt surgical treatment is important. Early motion exercises help minimize joint stiffness and do not seriously delay wound closure.

Accidents, Occupational↗
PubMed1979

Properties and function of fumarate reductase (NADH) in Streptococcus lactis.

The fumarate reductase (NADH) present in cell-free extracts of S. lactis C10 was purified approximately 100-fed by chromatography on DEAE-cellulose in the presence of the non-ionic detergent Teric X-10, and some of the properties of this partially purified enzyme were characterized. Fumarate was able to act as a terminal electron acceptor and decreased the amount of lactate formed and oxygen used during the metabolism of pyruvate by resting cells of S. lactis. Anaerobic growth of S. lactis on glycerol was not observed and fumarate reduction was not coupled with glycerol-3-phosphate oxidation.

Anaerobiosis↗
PubMed1978

Compartmentalization and growth of the Drosophila abdomen.

An analysis of the cell lineage of the adult Drosophila abdomen is reported. Genetically marked clones are produced in the tergites in the embryo and in the sternites and pleura during larval life. The spatial disposition of the segment primordia is also studied in a series of 250 gynandromorphs. We conclude that in the embryo the segments are all separate polyclones soon after blastoderm and these are probably adjacent to each other. Gynandromorph analysis suggests that segments 2--6 develop from similarly sized cell groups but that the first tergite develops from a primordium which is equal to that of two other segments. We suggest that presumptive adult and larval cells are not separated at blastoderm. Our estimate from clonal analysis of the number of larval cells (12) which construct the sternites and pleura is equal to the number observed directly in the ventral nest of histoblasts.

Abdomen↗
PubMed1975

The anatomy of a compartment border. The intersegmental boundary in Oncopeltus.

In the insect Oncopeltus (Hemiptera, Lygaeidae), after blastoderm formation, labeled cells in one segment never give rise to cells in another; clones always respect a sharply defined line, the segmental boundary. Similarly, demarcation lines defining "compartments" have been recently found within the imaginal disks of Drosophila and promise to be of first importance in developmental genetics. In Oncopeltus the segmental border is a straight line in a smiple epithelial monolayer and is marked by a change in pigmentation that is visible in the electron microscope. Reconstructions from serial sections show that there is a change of cell shape at the boundary, but attachment desmosomes, septate junctions, and gap junctions link cells of different segments as well as cells of the same segment. The form of the epithelium at different stages of the molt cycle is described, and the possibility that there may be an abrupt change of cell adhesiveness at the segment boundary is discussed.

Animals↗