[Biosynthesis of viral proteins].
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Arboviruses↗
Biomedical subjects
S M Akopian
Publications and source records attributed to S M Akopian.
[Bacillus thuringiensis plasmid DNA].
Plasmid DNAs were isolated from Bacillus thuringiensis and their physico-chemical properties were studied. The DNAs were purified using ultracentrifugation in a CsCl2 density gradient. The molecular weight of these extrachromosomal DNAs determined by electrophoresis in 0.6% agarose gel and by electron microscopy was 6 . 10(6)--10 . 10(6) daltons; a DNA with a molecular weight of 90 . 10(6) daltons was also found. Digestion with restriction endonuclease EcoRI yielded fragments of the plasmid DNAs with cohesive ends; these were further identified by means of electrophoresis in 1% agarose gel.
Bacillus thuringiensis↗
[Characteristics of Pseudomonas putida plasmid DNAs].
Physico-chemical characteristics of plasmid DNAs isoalted from Pseudomonas putida G7 were studied as well as the behavior of these DNAs in th eourse of chromatography on columns with Sepharose 4B and kieselguhr with methylated albumin (MAC). This strain was found to contain several plasmid DNAs having molecular weights of 33-36X10(6), 15-18X10(6), and 3-5X10(6) dalton. The plasmid DNAs of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is characterized by extrachromosomal localization.
Chemical Phenomena↗
[Structure of polylysine-DNA complexes].
By the methods of nuclease digestion and electron microscopy the complexes of poly-I-lysine with phage T2 DNA were studied. At micrococcal nuclease digestion of complexes polylysine--phage T2 DNA structures resistant to nuclease and containing fragments of DNA with lengths of about 300, 600, 1200 base pairs were revealed. On the electron micrographs structures superficially resembling nucleosomes were detected.
Base Composition↗