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Biomedical subjects

S Liu

Publications and source records attributed to S Liu.

At least 865 records · Page 48Linked to original sources
PubMed1995

[Comparison of toxic effects of brucite and aluminium-treated chrysotile on macrophage].

Toxicity of brucite and two kinds of chrysotile (Xinkang and Sichuan chrysotile) treated with aluminium chloride to alveolar macrophage from guinea pigs was compared in vitro. Results showed changes of toxicity, indicated as generation of O2-., increase in membrane lipid fluidity and leakage of intracellular potassium, etc., caused by aluminium-treated chrysotile, were significantly lower than those by bructite of same dose (P < 0.05 and P < 0.01, respectively), and changes of other toxic indicators, such as release of lactic dehydrogenase (LDH) and acid phosphatase (ACP) from macrophage and its mortality, were similar to those by brucite. It suggests aluminium chloride can be used to antagonize pathogenic bioactivity of chrysotile in practice.

Aluminum Chloride↗
PubMed1995

[Implementation of national tuberculosis control programme--focus on registration, case-management and treatment].

The results of three nationwide epidemiological surveys on tuberculosis showed the declination of the magnitude of tuberculosis from 1979 to 1990 was slower. The case registration rates and new case registration rates within ten years (1982-1991) were gradually increasing year by year and came to a climax in 1988. The fact indicated the improvement of case-finding and case registration. The case registration rate was going down thereafter coincided with the declination of the prevalence of tuberculosis. The proportion of registration rate to the prevalence was rather low. This is mainly attributed to the fact that the majority of detected patients were held by general medical clinics and had not been registered to the central registration of tuberculosis. Therefore, only 17.7% of detected tuberculosis cases were under proper case-management for treatment. The results of cohort analysis on smear-positive treated cases showed the rate of sputum negative conversion was gradually increasing and the case lost rate was going down. The case-management for treatment was improving year by year. The results of sociological study on tuberculosis indicated that the financial problem was the main factor inducing delay of treatment and intermission and interruption of treatment among treated patients.

China↗
PubMed1995

[Chemical constituents of Euphorbia nematocypha Hand.-Mazz].

Four compounds were isolated from the roots of Euphorbia nematocypha. Three of them were identified as A'-neogammacer-22(29)-en-3 beta-ol (18 beta.21 alpha) (II), jolkinolide E (III) and beta-sitoesterol (IV). Among them, II was obtained from the genus Euphorbia and III from this plant for the first time.

Diterpenes↗
PubMed1995

[Flow cytometry analysis of 67 squamous cell carcinoma of the tongue].

Flowcytometry was used to measure the DNA content in 67 cases with squamous cell carcinoma of the tongue. The relationship of FCM resulte are compared with clinical and pathological parameters. The results show that the recurrences are always appeared within 2 years after operation. Therefore, it is important to follow up patient for the first 2 years after operation. 75% of recurrences are aneuploidy. With multiple statistic analysis, only the clinical stage and aneuploidy are related to lymph node metastasis. The close relative factors to recurrence of SCCT are clinical stage, lymph node metastases, DI value and DNA ploidy.

Adult↗
PubMed1995

[Effects of hypoxia on the expression of platelet-derived growth factor (PDGF) gene and PDGF-B chain protein in porcine pulmonary artery endothelial cells].

Northern blot, slot blot hybridization and quantitative immunocytochemistry analyses were used to study the effects of hypoxia on the expression of PDGF gene and PDGF-B chain protein in cultured pulmonary artery endothelial cells (PAEC). Hypoxia (3% oxygen) significantly increased PDGF-B chain mRNA in PAEC, whereas mRNA level of PDGF-A chain was not increased significantly in comparison with normoxic control (21% oxygen). PDGF-B chain immunoreactivity on PAEC exposed to hypoxia was markedly stronger than that of normoxic PAEC and PDGF-B China immunoreaction. was found in cytoplasm and perinuclear region of the hypoxic PAEC, however, only in the nuclear region of normoxic PAEC. Increased expression of PDGF-B chain protein roughly paralleling that of PDGF-B mRNA was also observed. These results suggest that hypoxia may stimulate the expression of PDGF-B chain by PAEC in vivo and PDGF may play an important role in the vascular remodeling of hypoxic pulmonary hypertension.

Animals↗
PubMed1995

[Determination of the content of icariine in four Chinese patent medicines containing Epimedium brevicornum Maxim].

A new HPLC method has been developed for analysis of Icariine in four Chinese patent medicines. The symmetric peak of Icariine and good resolution were gotten, when ODS was used as analytical column (30 degrees C) MeOH-KH2PO4 (pH = 2.7, adjusted with H3PO4) (57:43) as a mobile phase; the detective wavelength was 268 nm. The contents of Icariine in Bushen-qiangshen-wan, Guiling-ji, Zhuang-gu-guanjie-wan and Qianliening-chongji were 0.105, 0.0995, 0.0566 and 0.313% respectively.

Chromatography, High Pressure Liquid↗
PubMed1995

[Effect of natural calcium against osteopenia in ovariectomized rats].

This study was designed to test the effect of natural calcium on the bone loss induced by ovariectomy in rats. Thirty female four-month rats were divided into three groups: sham operated group (Sham), ovariectomized group (OVX) and ovariectomized with calcium supplementation group (OVX + CA). OVX + CA rats were treated with calcium at a dose of 50 mg/kg weight per day for 90 days after surgery. Blood biochemical analysis, bone pathology, bone histomorphometric measurement and bone weight were processed. OVX rats were characterized by thinning, wormerosion like loss of trabecular bones, decreased volume of cancellous bone, increases in osteoblast index and osteoclast index, decreases in wet, dry and ash weights of left femur, and decreases in contents of calcium and phosphorus of bones. Treatment of OVX + p6 rats with calcium provided complete protection against bone loss and significantly depressed bone turnover. Our results indicate that natural calcium is effective in prevention of osteopenia in ovariectomized rats. The authors believe that the main mechanism of calcium against bone loss exists in correction of minus calcium balance, and depression of high bone turnover, especially the absorption action of osteoclasts.

Alkaline Phosphatase↗
PubMed1995

[Comparative study of umbilical artery Doppler velocimetry, antenatal fetal heart rate monitoring and umbilical artery blood-gas analysis in the prediction of neonatal outcome].

One hundred and fifteen term pregnancies (62 normal pregnancies, 53 high risk pregnancies) were observed by umbilical artery Doppler velocimetry (UmA S/D ratio) and antenatal fetal heart rate monitoring (NST), and 37 of them were also observed by umbilical artery blood-gas analysis at birth. We compared the effects of these three methods to predict the neonatal outcome. The standards of the neonatal poor outcome were 5-min Apgar score < or = 7 and/or a birth weight < 2500g (SGA). The results were: (1) The neonatal poor outcome were more frequently observed in the abnormal UmA S/D ratio (> or = 3.0) group and in the UmA pH < or = 7.2 group (P < 0.005, P < 0.025, respectively), but there was no significant difference between the NST reactive or nonreactive groups (P > 0.05); (2) The neonatal acidosis at birth was also associated with the abnormal UmA S/D ratio (> or = 3.0); (3) The sensitivities of UmA S/D ratio, UmA pH, NST to predict the 5-min Apgar score < or = 7 were 60.0%, 60.0%, 20.0% respectively; the specificities 84.6%, 81.3%, 81.8% respectively. And their sensitivies to predict SGA were 100.0%, 100.0%, 40.0% respectively; the specificities 86.4%, 80.0%, 82.7% respectively. Our data show that UmA S/D ratio and UmA pH are better than NST in the prediction of neonatal outcome, and the abnormal UmA S/D ratio is closely associated with neonatal acidosis at birth.

Apgar Score↗
PubMed1995

Analysis of C-Ha-ras gene amplification and mutation in laryngeal carcinoma.

In order to study the altered molecular events during laryngeal carcinogenesis and elucidate the role of Ha-ras oncogene amplification and mutation, we have examined their profile by polymerase chain reaction (PCR) and selective oligonucleotide hybridization. We analyzed the mutational status of codon 12 of Ha-ras in 22 laryngeal carcinomas and 10 normal tissues, and found that 7 of 22 laryngeal carcinomas contained a Ha-ras mutation at codon 12. The frequency of mutation was 32%. None of the normal tissues revealed mutation. Moreover, no amplification was found in cancers when compared to the normal. Our findings indicated that the activated Ha-ras gene existed in laryngeal carcinoma, and activation of the Ha-ras gene by mutation at codon 12 might play a key role in laryngeal carcinogenesis.

Carcinoma, Squamous Cell↗
PubMed1995

[Malignant rhabdoid tumor of kidney: a clinicopathologic and immunohistochemical study of 15 patients].

The clinical and pathological features of 15 infants with malignant rhabdoid tumors of kidney are presented. These tumors were identified among 391 primary renal neoplasms in this hospital. The male/female ratio was 2.8:1.0, mean age at diagnosis was 18 months with a range from 4 to 55 months. Of the 10 patients with follow-up records, 8 have died, 2 were alive and free of disease 15 and 55 months after the diagnosis respectively. A wide histologic spectrum was encountered. All tumors exhibited classical morphology in at least some areas, characterized by solid proliferation of monotonous tumor cells with vesicular nuclei and prominent nucleoli, abundant cytoplasm and intracytoplasmic inclusions. Immunohistochemical studies were performed in all 15 cases. Vimentin was positive in all tumors, ENA in 12, cytokeratin in 8, desmin in 2 and myoglobin in one. All cases were negative for presence of lysozyme, NSE and neurofilament. Our results show that this tumor is a distinctive and highly malignant neoplasm of the infant kidney with considerable morphological and immunohistochemical diverse phenotypes.

Child, Preschool↗
PubMed1995

Three-dimensional structure of CA1 pyramidal cells in rat hippocampus--optical recording of LSM and computer simulation of fractal structure.

The optical recording of three-dimensional (3-D) reconstruction of CA1 pyramidal cells was derived from the studies on the CA1 region of the hippocampus in adult male Wistar rats. The recording was produced by the Confocal Laser Scan Microscope (LSM-10). The attemption was to outline the morphological neural network of CA1 pyramidal cells organization, following the trail of axo-dendritic connections in 3-D spatial distributions among neurons. The fractal structure of neurons with their dendritic and axonal trees using fractal algorithm was noticed, and 2-18 simulated cells were obtained using PC-486 computer. The simulational cells are similar in morphology to the natural CA1 hippocampal pyramidal cells. Therefore, the exploitation of an advanced neurohistological research technique combining optical recording of the LSM-10 and computer simulation of fractal structure can provide the quantitative fractal structural basis for chaosic dynamics of brain.

Animals↗
PubMed1995

[Surgical treatment of 165 cases of stage III small cell lung carcinoma].

The author analysed the effect of surgical therapy in 165 cases with stage III SCLC from 1979 to the end of 1991, and compared with 87 cases of combined therapy and 78 cases in simple operative therapy. The author also makes an observation of cases with pre-operative and (or) post-operative chemo- and radiation therapy. Their over-all 5 year survival rate was 16.6%. Stage among 5 year survival cases in TNM stage. IIIa was 20.4% and no for Stage IIIb. The 5 year survival of combined therapy was 22.8% and that of simple operative group was 7.9%. The result shows that in the Stage III SCLC combined therapy is superior to that of simple operative group. It seems that postoperative radiation and chemotherapy is better than pre-operative in combined therapy group. The author's opinion is that surgical indication of SCLC is limited to stage IIIa.

Adult↗
PubMed1995

[Comparison of in-vitro and in-vivo activities of enoxacin and ofloxacin against the Mycobacterium tuberculosis].

Enoxacin (ENX) showed a moderate degree of activities in vitro against Mycobacterium tuberculosis in modified Saunton medium and modified Lowenstein-Jensen Medium with MICs from 1 to 8micrograms/ml. Ofloxacin (OFLX) is superior to ENX, with MICs from 0.5 to 2.0micrograms/ml. In terms of median survival time (ST50), ENX at doses both 3mg (about 150mg/kg) daily and 6mg (about 300mg/kg) intermittently were inactive (P > 0.05) against M. tuberculosis infection in mice. OFLX at the same doses all were very significantly effective (P < 0.01).

Animals↗
PubMed1995

Multimodality therapy including surgical resection for limited small cell lung cancer.

From 1975 through 1990, 199 patients with limited small cell lung cancer (LSCLC) were subjected to multimodality treatment including surgical resection combined with chemotherapy or chemoradiotherapy in our department. The median postoperative survival time of the 199 patients was 39 months, and the 5-year survival rate was 26%, which was decreased with increase of tumor-stage. In comparison of the survival time of patients in Stage I and those in Stage IIIa, there was a significant difference (P < 0.01). There were no significant differences in survival rate of 3 and 5 years between the patients receiving chemotherapy prior to or after surgical resection. The improvement in survival was documented by surgical resection combined with chemotherapy or chemoradiotherapy for LSCLC. The effect of multimodality treatment is correlated with tumor P-TNM staging, the involvement of lymph node, especially that of the mediastinal lymph node, is a negative factor influencing the prognosis. Surgical resection is an initial management, followed by chemotherapy or chemoradiotherapy may be indicated in LSCLC patients of Stage I, Stage II and some Stage IIIa as the cancer can be resected completely.

Adult↗
PubMed1995

Characteristics of an established retinoblastoma cell line HXO-Rb44.

PURPOSE: To study the retinoblastoma cell culture and to establish a new retinoblastoma cell line. METHODS: 22 retinoblastomas were cultured by using the method of single cell suspension. Characteristics of the cultured cells were studied in the following programs: tumor cell morphology in vitro, electron microscopic, growth curve, cloning in soft agar, immunohistochemistry, karyotype and tumorigenicity. RESULTS: 22 retinoblastomas were cultured successfully in vitro, only a continued cell line HXO-Rb44 was established (more than 3 years). The characteristics of this cell line are that it grew as a suspension of round cell in graps like clusters in vitro, its population doubling time was 44 hours, and it could be cloned in soft agar. Histopathologic and ultrastructured pictures showed the characteristics of Rb. HXO-Rb44 cell was positive to NSE and negative to GFAP in immunohistochemical staining. A subcutaneous injection of HXO-Rb44 cells produced a retinoblastoma in BALB/C athumic nude mice. CONCLUSIONS: HXO-Rb44 has the characteristics of retinoblastoma and is a new retinoblastoma cell line. It is a useful material for study this tumor both in basic and clinical fields.

Animals↗
PubMed1995

[Effects of resuscitation with hypertonic sodium lactate dextran 70 on cardiac function in severely burned dogs].

This comparative study was performed to evaluate the effects of resuscitation of burn shock with hypertonic sodium lactate dextran 70(HLD solution: Na+250 mmol/L and 6% dextran 70) and lactate Ringer' solution on cardiac function, lipid peroxide and SOD activity of heart tissue. Dogs with 35% TBSA third degree burns received, one hour postburn, either HLD resuscitation (HLD group, n = 6) in the amount of 19.6 ml.kg-1/g hours, followed by LR 6 ml.kg-1/% TBSA, or LR resuscitation (LR group, n = 6) in the amount of 8 ml.kg-1/% TBSA LR solution. The results of this experiment showed that cardiac index (CI) of HLD group was much higher than that of LR group at 4, 8, 12, 24 hours postburn. The dp/dt max and-dp/dt max of HLD group were much higher than that of LR group at 12.24 hours postburn. Level of MDA in heart tissue of HLD group (1.74 +/- 0.28 10 mol/g heart tissue) was much lower than of LR group (3.23 +/- 0.56 10 mol/g heart tissue) at 24 hours postburn (P < 0.01). Meanwhile, SOD activity in heart tissue of HLD group (157.49 +/- 32.23 u/mg heart tissue) was much higher than that of LR group (46.88 +/- 16.28 u/mg heart tissue) (P < 0.01). These results suggested that HLD resuscitation might be beneficial in improving postburn cardiac function and attenuating postburn oxidant-induced lipid peroxidation in heart tissue not only by its "oxygen scavenger" effect, but also by its elevation of SOD activity of heart tissue.

Animals↗