Biomedical subjects

S Liu

Publications and source records attributed to S Liu.

At least 361 records · Page 20Linked to original sources

PubMed2000

[Tissue engineered neo-cartilage using polymer substrates and chondrocytes: experimental study].

OBJECTIVE: To investigate the possibility of using poly beta-hydroxybutyrate (PHB) as a scaffold for chondrocytes and the feasibility of generating tissue engineered cartilage in rabbit animal model. METHODS: The articular cartilage from 4-week-old rabbits was enzymatically dissolved by type II collagenase, and chondrocytes were harvested and seeded into foams of PHB. After incubation for 1 week in vitro, chondrocyte-polymer constructs were implanted subcutaneously into rabbits. Two respective control groups were established by subcutaneous implantation of PHB foams and chondrocytes alone. The rabbits were killed at 4, 8, 12 weeks postoperatively, and the specimens were dissected and examined macroscopically, histologically and immunohistochemically. RESULTS: Specimens harvested from chondrocyte-polymer constructs and subjected to gross morphologic and histologic analysis demonstrated new cartilage formation, and those from control groups showed no cartilage formation. CONCLUSION: These findings demonstrate that the foam of PHB is not only a good "matrix" for cartilage tissue engineering, but also optimal scalffold for the seeding of chondrocytes to generate new cartilage that would be useful in plastic and reconstructive surgery.

Animals↗

PubMed2000

[Genetic polymorphisms of Rb and susceptibility of esophageal cancer].

OBJECTIVE: To assess the possible association between the polymorphisms of Rb (intron 20, 17) and susceptibility of esophageal cancer so as to provide clues for genetic markers of esophageal cancer. METHODS: Samples were taken from 56 normal Han people of Shaanxi, 47 esophageal cancer samples, and 31 peri-cancerous non-tumor. DNAs were analysed by using PCR technique. RESULTS: Ten alleles fragments were found in Han population of Shannxi by using primer Rb gene (intron 20). PIC was 0.86. Heterozygosity was 0.7. In esophageal cancer tissues, PIC was 0.82. Heterozygosity was 0.55. In peri-cancerous non-tumor, PIC was 0.87. Heterozygosity was 0.74. Distribution of genotype frequency was significantly different between Han population of Shaanxi and esophageal cancer (P < 0.01). Two alleles (945 bp, 630 bp + 315 bp) fragments were found in Han population of Shaanxi by using primer Rb gene (intron 17). Genotype frequency of Han population of Shaanxi was A/A: 0.28, A/B: 0.52, B/B: 0.20. Genotype frequency of esophageal cancer was A/A: 0.11, A/B: 0.77, B/B: 0.12. Distribution of genotype frequency was significantly different between Han population of Shaanxi and esophageal cancer (P < 0.05). Mutation of Rb gene was found in 7 cases. CONCLUSIONS: Polymorphism of Rb (intron 20) VNTR and Rb (intron 17) could be used as valuable markers. There should bea relationship between polymorphism of Rb VNTR and esophageal cancer susceptibility.

Esophageal Neoplasms↗

PubMed2000

[Relationship between tumor suppressor gene p16 and Rb and early diagnosis of lung cancers].

OBJECTIVES: To study the incidences and mechanisms of tumor suppressor gene p16 and Rb inactivation in lung cancers, and to investigate their relation to biological characters, clinical pathological diagnosis and gene classification diagnosis of lung cancers. METHODS: Immunohistochemistry, double in situ hybridization, PCR, PCR-SSCP and sequencing were applied to study the changes of p16 and Rb genes in 106 lung cancers and 23 lung tissues of benign diseases. RESULTS: Total expression rates of p16 and Rb proteins and mRNAs in lung cancers were lower than those in normal lung tissues and benign lung diseases, and they were specially related to histological type, metastasis and clinical stage of lung cancers. In stage I and II lung cancers, the obvious inactivation of tumor suppressor gene p16 or Rb was examined (32.6% or 28.3%); p16 inactivation was detected mainly in non-small cell lung cancers, and Rb inactivation mainly in small cell lung cancers. There were three mechanisms of homozygous deletions, methylations and mutations for p16 gene inactivation. The deletion rate of p16 exon1 and/or exon2 was 25.8%, mainly took place in p16 protein negative cases of non-small cell lung cancers. 15 cases (16.9%) took part in methylations on SmaI sites of CpG island of p16 gene. PCR-SSCP and sequencing showed that 9 cases had p16 gene mutations. CONCLUSIONS: p16 and Rb genes may play important roles in genesis and progression of lung cancers. Inactivation of p16 or Rb gene may be is an early link of lung canceration, that is very important for early diagnosis of lung cancers. A new gene classification model for lung cancer diagnosis would be set up based on the research of p16 and Rb genes.

Adolescent↗

PubMed2000

Relations of tumor suppressor gene p16 and RB to early diagnosis of lung cancer.

OBJECTIVES: To study the incidence and mechanism of the tumor suppressor gene p16 and RB inactivation in lung cancer, and to investigate their relations to biological characteristics, clinical pathological diagnosis and gene classification. METHODS: Immunohistochemistry, double in situ hybridization, PCR, PCR-SSCP, and sequencing were used to study the changes in the p16 and RB genes in 106 patients with lung cancer. The specimen of normal lung tissue from the patients with lung cancer and specimens of the 23 patients with benign lung diseases were studied in contrast to those of the same patients. RESULTS: The total expression rate of the p16 and RB genes in the lung cancer tissues of the 106 patients was lower than that in normal lung tissues and tissues of benign lung diseases and was specially related to histological type, metastasis, and clinical stage of lung cancer. In stage I and II lung cancer, the obvious inactivation rate of the tumor suppressor gene p16 or RB was 32.6% or 28.3%. The p16 inactivation appeared largely in non-small cell lung cancer (50.1%), and the RB inactivation largely in small cell lung cancer (88.2%). Homozygous deletion, methylation, and mutation were three mechanisms for the p16 gene inactivation. The deletion rate of the p16 exon 1 and/or exon 2 was 25.8% in the patients with non-small cell lung cancer. Fifteen patients (16.9%) showed methylation at the SmaI sites of CpG island of the p16 gene. PCR-SSCP and sequencing revealed p16 gene mutation in 9 of the 106 patients with lung cancer. CONCLUSIONS: The p16 and RB genes may play an important role in genesis and progression of lung cancer. The inactivation of the p16 or RB gene may be an early sign of lung canceration, that is important for the early diagnosis. A new gene classification model for lung cancer diagnosis would be set up based on the research of the p16 and RB genes.

DNA Methylation↗

PubMed2000

[Protective effect of nitric oxide on pancreas and its relation to sulfhydryl compounds and oxygen free radicals].

OBJECTIVE: To investigate the effect of endogenous nitric oxide (NO) on acute necrosis pancreatitis in rats and its relation to sulfhydryl compounds and lipid peroxidation. METHODS: Acute necrosis pancreatitis in rats was induced by retrograde sodium taurocholate (5%) infusion into the pancreatobiliary duct (1 ml/kg body weight), and N(G)-nitro-L-arginine (L-NNA) was used as the inhibitor of endogenous NO. The effect of endogenous NO on pancreatic injury, serum amylase level, the pancreatic tissue levels of sulfhydryl compounds, and malonaldehyde (MDA, the end product of lipid peroxidation) was evaluated, respectively. RESULTS: Sodium taurocholate administration induced evident pancreatic tissue edema and acinar necrosis, and intrapancreatic hemorrhage occurred in 2/7 rats. Both serum amylase and tissue MDA [(1.25 +/- 0.28) nmol/mg x pr vs. (0.5 +/- 0.03) nmol/mg x pr, P < 0.05] were significantly increased, but tissue sulfhydryl compounds were decreased markedly. Pretreatment with the NO inhibitor, L-NNA (12.5 mg/kg body weight), significantly intensified acinar necrosis and increased the intrapancreatic hemorrhage (10/12). L-NNA also resulted in a further increase of serum amylase and tissue MDA [(3.0 +/- 0.40) vs. (1.25 +/- 0.28) nmol/mg x pr, P < 0.05], but it had no effect on the tissue sulfhydryl compounds. CONCLUSION: Endogenous NO has the effect of pancreatic protection, and its antioxidation may be responsible, at least in part, for the protective mechanisms. Sulfhydryl compounds may not be involved in NO's pancreatic protection mechanisms.

Amylases↗

PubMed2000

[The survey of prevalence of HIV infection among paid blood donors in one county in China].

OBJECTIVE: To estimate the prevalence rate of HIV infection among paid blood donors and to analyse high risk factors. METHOD: All residents between 18 to 60 years old were selected from on county in March 1999. RESULTS: There were 210 paid blood donors among 730 interviewed subjects. The HIV prevalence rate among paid blood donors was higher (9.1% or 19/210) than that of others (0.6% or 3/520). The HIV prevalence rate was higher in plasma donors (25.9%) than that (2.6%) among 210 blood donors. Donors who donated blood/plasma more than 10 times each year had a higher prevalence rate (13.5%) than those who donated less than 10 times each year (2.8%). Donors who had donated blood but stopped donation before 1995 had a higher prevalence rate (18.3%) than those who started donation after 1996 (1.4%). The results of KAP survey showed that blood donors had a low knowledge about AIDS. CONCLUSION: There was a higher prevalence rate of HIV infection among paid blood donors in some regions. It should take measures to prevent further transmission HIV through paid blood donors.

Adolescent↗

PubMed2000

[Establishment and initial utilization of an arithmetic model of chronic hepatic diseases].

OBJECTIVE: An arithmetic model was established to reflect the natural development of chronic hepatitis B, and to help decision making in public health. METHODS: The frame of this model was composed of 6 states - chronic hepatitis B (CHB), natural recovery (NR), compensated cirrhosis (CIR), decompensated cirrhosis (DEC), hepatocellular carcinoma (HCC) and death (DEA). It was supposed that each state would develop into the other possible states at different rates after different years. According to many related reports, 15 values or functions of transfer rates were calculated, and on the ground of them the present model - the Foxpro program was established. Hypothetical CHB and other patients had been introduced to simulate the natural course over 55 years. RESULTS: It was shown that the observation period when 50% of NR, CIR, DEC and HCC were developed from CHB were 3, 11, 20 and 24 years respectively. The CHB, CIR, DEC and HCC patients could last 30, 12, 4 and less than 1 year respectively before half of them died. At the 30(th) year, proportions of death were 41.3% from HCC, 33.0% from DEC and 25.7% from the other causes. CONCLUSION: After the comparison between the main results of the model and real reports from the past years, it could be concluded that the model was reliable.

Hepatitis B, Chronic↗

PubMed2000

[The first discovery of endemic Lyme disease in Shandong province].

OBJECTIVE: To investigate the endemic area of Lyme disease in Shandong province. METHODS: An investigation on endemic Lyme disease was conducted by means of serological and etiological methods in Shandong province from 1992 to 1999. RESULTS: Of 1919 forestry residents, 120 residents had a significant antibody titer against Borolo burgdorferi strain B(31). The prevalance rate of Lyme disease was 6.25%. Haemaphysalis longicornis was a prevailing species of Ixodidae in the region. Spirochetes were observed in the midguts of 50 H. longicornis in this area by direct fluorescence antibody method. The overall positive rate was 12.0% (6/50). Two strains (TSH(1), TSH(3)) of spirochetes were isolated from H. longicornis. Both TSH(1) and TSH(3) were positively responded to McAb H(5332) and H(9724), but negatively to H(6831). Spirochetes were observed in the kidneys of 46 Rattus in this area by direct fluorescence antibody method with a positive rate of 13.26% (6/46). CONCLUSION: It was the first time that the mountainous areas in Shandong province was confirmed endemic areas of Lyme disease.

Animals↗

PubMed2000

[Analysis on the causes of burn among 17,339 patients].

OBJECTIVE: To find out the common causes of burn and to develop preventive measures to prevent or reduce the injury of burn. METHODS: Descriptive analysis was used to review the burn-cases in our Burn Department from 1970 to 1998 and to summarize the epidemic features and common causes of the injuries. RESULTS: From 1970 to 1998, a total number of 17 339 inpatients were seen in our Burn Department. According to the causes of injury, burn patients were categorized as heat-injured, electric-injured, chemical-injured, etc. Most patients were injured with heat, taking up 89.11% of the total patients (P < 0.01). Most of the heat-injured patients were scalded with hot water (49.31%, P < 0.01). Children being burnt, accounted for 32.82% of the total patients (P < 0.01), whom should not be ignored. CONCLUSION: Burn happens frequently in daily life, so the preventive methods should be taken seriously.

Adolescent↗

PubMed2000

[Detection of numerical chromosome aberrations in sperm of workers exposed to benzene series by two-color fluorescence in situ hybridization].

OBJECTIVE: To study the numerical aberrations of sex chromosomes in sperm of workers exposed to benzene series. METHODS: The interphase sperm from workers of exposed and control groups were hybridized using two color fluorescence in situ hybridization with X and Y(alpha)-satellite chromosome specific DNA probes, and the aneuploidy frequencies of X and Y chromosome in X and Y sperms were detected simultaneously. RESULTS: Geometric mean concentration of benzene in workplace air was 83.95 mg/m(3), one fold higher than national maximum allowable concentration. A total of 74,517 sperm nuclei in 13 benzene-exposed workers and 89,662 sperm nuclei in 13 control workers were counted. Frequencies of disomic sperm for X, Y and XY were (0.15 +/- 0.05)%, (0.13 +/- 0.05)% and (0.20 +/- 0.11)% in workers exposed to benzene series, and (0.10 +/- 0.04)%, (0.10 +/- 0.04)% and (0.13 +/- 0.06)% in control workers. There was statistical difference in disomic frequency of X sperm and overall disomic frequency between the benzene series-exposed workers (0.15% and 0.48%, respectively) and the control workers (0.10% and 0.33%, respectively). CONCLUSION: Exposed to benzene at higher concentration may induce increase in aneuploidy frequency of sperm sex chromosome in exposed workers.

Adult↗

PubMed2000

[Effect of chrysotile and cigarette smoking solution on DNA damage and repair in human alveolar epithelial cells].

OBJECTIVE: To study the effect of chrysotile and solution of cigarette smoke separately or combined on DNA damage and repair. METHODS: Single-cells gel electrophoresis assay was used to detect break in DNA strand and to analyze its repair function in human alveolar epithelial cell line A549, which were exposed to chrysotile or cigarette smoke solution separately or combined for one hour and then immediately cultured for different time. RESULTS: Break in DNA strands was induced obviously in A549 cells after exposure to 40 microg/ml of chrysotile or 2.5 x 10(-4) cig/ml of cigarettes smoke solution for one hour. Repair for DNA break could be seen clearly in chrysotile-treated cells after continuing culture for three hours, and 30.6% had been repaired after culture for four hours. Repair could also be seen clearly in cigarette smoke solution-treated cells after continuing culture for two hours, and 65.2% had been repaired after culture for four hours. Repair of DNA in cells exposed to both chrysotile and cigarette smoke solution combined was similar to that exposed to cigarette smoke solution only. CONCLUSION: Damage to DNA caused by chrysotile is more difficult to repair than that induced by cigarette smoking solution.

Asbestos, Serpentine↗

PubMed2000

[Studies on chrysotile-induced cell transformation and its intervention effect].

OBJECTIVE: To explore the effects of several kinds of chemicals on chrysotile-induced cell transformation in human embryo lung (HEL) cells. METHODS: After being soaked in aluminum citrate, mixed rare earth or sodium selenite solutions for one hour, chrysotile was incubated with HEL cells. Then, the number of focus of cell transformation was observed. RESULTS: Chrysotile could cause morphological transformation of HEL cells in a dose-dependent pattern, with (0.60 +/- 0.55), (1.20 +/- 1.31), (2.60 +/- 1.14) and (3.60 +/- 1.14) foci of HEL cell transformation per plate after treatment with 0.625, 1.25, 2.5 and 5.0 microg/ml of chrysotile, respectively, and there was significant difference between the groups treated with 2.5 and 5.0 microg/ml of chrysotile and control group. It indicated that chrysotile could cause morphological transformation of HEL cells. Number of focus of HEL cell transformation induced by chrysotile that was pretreated with the above-mentioned three kinds of chemicals reduced significantly, as compared with that in the group without pretreatment of it. Its inhibitory rate was 50% in the group treated with mixed rare earth, with significant difference. CONCLUSION: Pretreatment for chrysotile with these three kinds of chemicals could reduce its carcinogenicity.

Asbestos, Serpentine↗

PubMed2000

[Studies on cell transformation and cell cycle in human embryo lungs induced by nickel compounds].

OBJECTIVE: To elucidate the association between nickel compounds and occupational lung cancer. METHODS: Human embryo lung cells (MRC-9 and IMR-90) were transformed by water-insoluble nickel oxide and nickel subsulfide, and changes in cell cycle of these transformed cells were analyzed by flow cytometric technique. RESULTS: Morphological transformation in MRC-9 and IMR-90 cells were induced after exposure to nickel compounds for 3 - 5 months, including formation of transforming foci, overlaying growth and epithelium-like changes in cells, etc. Some transformed cells could form colonies in soft agar media. Changes in cell cycle of the transformed cells were observed with flow cytometry. Proportions of cells at G(1) phase and G(2)/M phase were 62.61% and 16.37%, respectively, in the untreated MRC-9 cells and hyperploid cells were hardly seen in cell cycle plots. But, in the transformed MRC-9 cells, proportion of cells at G(1) phase reduced to about 50%, and that at G(2)/M phase increased to over 20% and that of hyperploid cells to over 10%. The same phenomenon was observed in the IMR-90 cells. CONCLUSIONS: Water-insoluble nickel compounds can induce morphological transformation of human embryo lung cells and those transformed cells appeared certain biological characteristics of tumor cells.

Cell Cycle↗

PubMed2000

[Further investigation on Second Volume of Essay on Simple Recipe and Pulsology (SESR) and Wang's Simple Recipes (WSR)].

There are 2 extant editions of SESR, written in Chunyou Jiachen (1244) reign. In the facsimile copy of Song manuscript recorded in Kojima's Records of Investigating Ancient Classics which was brought by Yang Shoujing and is now collected in the Library of Palace Museum in Taipei. There is also a facsimile copy of Song dynasty postscribed by Taki Genkan now collected in Library of Relics and Naturalist Academy of National Imperial Secretariat in Japan. The book is written in one volume, dealing first with four diagnostic methods, followed by drug and therapy and compounding of recipes and "processing" of drugs, thus forming a complete system. The WSR included in Classified Collection of Recipes is not the Essay on Pulsology, neither WANG Shou's Simple Recipes, but Yi jian gui yi (Unity of Simplicity and Easiness) by XU Ruoxu of Yuzhang, Jiangxi of Yuan dynasty, being an epitomized work of all physicians of Yongjia medical schools with only 3 pieces of incomplete papers remained.

China↗

PubMed2000

[The effect of burn sera on the calcium homeostasis in isolated rat myocardial cells].

OBJECTIVE: To study the effect of burn sera on the calcium homeostasis in isolated rat myocardial cells. METHODS: The burn sera were obtained from the Sprague -- Dawley (SD) rats inflicted by 30% TBSA III degree burn at 2, 4 and 6 postburn hours with the harvesting of burn sera at different postburn times, named as 2 hBS, 4hBS and 6 hBS, respectively. Myocardial cells were isolated by enzyme digestion with retrograde perfusion apparatus. [Ca(2+)]i was detected with the fluorescent dye Fura -- 2 and L -- type calcium (ICa -- L) currents were recorded in the whole -- cell patch -- clamp model. RESULTS: Myocardial cellular [Ca(2+)]i became much higher in concent after being in cubated with sera, especially with 6hBS, than that in normal myocardial cells (P < 0.01). But the effect of 6hBS could be significantly inhibited by verapamil (30 nmol/L), the calcium channel antagonist and procaine (2 mmol/L), the inhibitor of rynodine receptor (P < 0.01) by 47.7% and 67.6%, respectively. Ca -- L was evidently increased by the stimulation of 2 hBS and 6 hBS, by 50 -- 80% and 1.5 -- 2.5 folds, respectively. It was indicated by I -- V curves that every ICa caused by depolarizaton and clamp voltage was increased by burn sera, which further made the maximal activating voltage ahead of time. All the burn sera effects on calcium current could be removed by extracellular lavage. CONCLUSION: The myocardial [Ca(2+)]i and ICa -- L could increase significantly when affected by burn sera. The alteration in calcium homeostasis might be a cellular mechanism of myocardial injury.

Animals↗

PubMed2000

[Establishment and biological characteristics of a novel erythroleukemia cell line (HIE1)].

OBJECTIVE: To establish a novel leukemia cell line and characterize its biological characteristics. METHOD: The cell line was established by liquid cell culture. The genetic marker was analyzed by R-banding and reverse transcriptase-polymerase chain reaction (RT-PCR), cell morphology by microscopy, electron microscopy and histochemical staining, cell surface antigen by monoclonal antibody, hemoglobin by hyperomethemoglobin measurement and electrophoresis, erythroid differentiation by benzidine-staining, and monocyte-macrophage differentiation by cell morphology and phagocytosis. RESULTS: A novel erythroleukemia cell line (HIE1), with original cell genetic marker (Ph chromosome, bcr/abl fusion gene rearrangement), was established from a CML patient in blast crisis, and has been passaged for over 60 generations. Myelomonocyte marker and hemoglycoprotein A were found on the cell surface. HIE1 cells contained hemoglobin, the same HbA and HbA(2) bands as in normal individuals were displayed by Hb electrophoresis. The benzidine positive HIE1 cells were induced after exposure to 3.6 x 10(-4) mmol/L Ara-C. When HIE1 cells were treated with 100 ng/ml PMA for 3 days, one third of the cells became spindle in shape, and 6.5% of the cells exert phagocytosis. The cells were classified into two types with Wright-staining: one showing light blue cytoplasm and a few of cells with basophilic granules, the other showing dark blue cytoplasm with vacuoles and pseudopods without granules. In addition, POX, SB, CE stains were negative, and AE, PAS, ACP stains positive. Colony formation of the cells was 37%, the cell doubling time was 22 - 24 hrs, and EB virus detection was positive. CONCLUSION: A novel erythroleukemia cell line with bcr/abl fusion gene and characteristics of myelomonocytic and erythroid cells was established.

Cell Differentiation↗

PubMed2000

[Evaluation of modified maze procedure by Doppler tissue imager].

Since 1998 the cardiac surgeons of WCUMS have performed modified maze procedure for treating the patients with rheumatic mitral valve disease accompanied with chronic atrial fibrillation (Af). In this study, echocardiography, especially Doppler Tissue Imager (DTI), was used to evaluate atria contraction and function of such patients before and after the operation. 14 rheumatic mitral disease patients, aged 27-60 years, were chosen; among them, 11 had mitral stenosis, 3 mitral regurgitation. The control group consisted of 16 cases, aged 24-67 years, with normal echocardiogram. Before and half-1 year after the operation, left atrium, left ventricle, right atrium and ventricle were measured, and cardiac function and posterior wall motion of left atrium were measured. Velocity of mitral valve annual and tricuspid valve annual was measured in DTI. The results showed: There was no age difference between the two groups. The motion of posterior wall of left atrium was 4.18 +/- 0.37 mm in the operation group, 4.09 +/- 0.29 mm in the control group (P > 0.05). No difference in velocity of mitral/tricuspid valve annual between the two groups was observed; 3 peaks of mitral valve annual were S 0.11 +/- 0.02, e 0.11 +/- 0.01, a 0.09 +/- 0.01 (m/s) in operation group, and S 0.08 +/- 0.00, e 0.10 +/- 0.01, a 0.08 +/- 0.01 (m/s) in control group, (P > 0.05, respectively); 3 peaks of tricuspid valve annual were S 0.10 +/- 0.01 vs 0.11 +/- 0.08, e 0.11 +/- 0.08 vs 0.10 +/- 0.01, a 0.10 +/- 0.10 vs 0.09 +/- 0.01 (m/s), (P > 0.05, respectively). The diameter of left atrium after operation in cases of mitral stenosis decreased significantly, compared with that before operation (P < 0.01). The diameter of right atrium after operation decreased significantly, compared with that before operation (P < 0.01). There was no significant change in right ventricle/left ventricle/EF and FS. The diameter of left atrium after operation in cases of mitral regurgitation decreased significantly, compared with that before operation (P < 0.01). The diameter of left ventricle after operation decreased significantly, compared with that before operation (P < 0.05). No significant change in right ventricle/right atrium/EF and FS was noted. The authors conclude the DTI, as a new diagnostic method in echocardiography, has high sensitivity and is less influenced when used to observe motion of atrium-ventricle annual influenced. It can avoid the error caused by deviation of blood displacement. In the rheumatic mitral valve disease patients who had mitral valve replacement and modified maze procedure, atrial contraction respores, the risk of thromboembolism decreases and prognosis improves. So the operation is worth doing.

Adult↗

PubMed2000

[Evaluation on antiendotoxic action and antiviral action in vitro of tetraploid Isatis indigotica].

OBJECTIVE: To evaluate the pharmacological effect of tetraploid Isatis indigotica Fort. METHODS: Comparing these actions of tetraploid I. indigotica with those of the diploid parent. RESULTS: These actions of tetraploid I. indigotica were obviously stronger than those of the diploid parent. The pharmacological effect of leaves was greater than that of roots with identical ploidy. CONCLUSION: Tetraploid I. indigotica is a new line of better quality, deserving to be exploited and utilized on a large scale.

Animals↗