Biomedical subjects

S Li

Publications and source records attributed to S Li.

At least 1,081 records · Page 60Linked to original sources

PubMed1997

[Study on the contact allergen in 720 patients with dermatitis and eczema].

In order to investigate the causative factors of dermatitis and eczema, patch test was performed using the GB7804-87 standard allergens in 720 patients with dermatitis and eczema. There were 376 patients reacted to one or more allergens. The total positive reaction rate was 52.2%. Positive reactions were most frequently with cobaltsulphate (118 cases, 16.4%), potassium dichromate (115 cases, 16.0%), nickelchloride (108 cases, 15.0%) mercuric chloride (106 cases, 14.7%), Beihai detergent powder (63 cases, 8.8%), and p-phenylene diamine (50 cases, 6.9%). The positive rate was above 1% in 18 allergens. This study shows that the standard screening allergens are valuable in the investigation of the aetiology of the dermatitis and eczema.

Adolescent↗

PubMed1997

[The effect of protheo on pulmonary function and the change of its plasma concentration in patients with chronic asthmatic bronchitis].

OBJECTIVE: In order to study the effect of protheo on pulmonary function and the change of its plasma concentration in patients with chronic asthmatic bronchitis. METHOD: 31 patients with chronic asthmatic bronchitis were treated by taking protheo 400 mg once a day orally for 6 weeks. The pulmonary functions, including FEV1, FVC, FEV1/FVC and PEFR, and the plasma concentrations of theophylline were measured respectively before and after the administration. RESULTS: The results showed that the pulmonary functions after the therapy were greatly improved compared with that before the therapy, even after the administration for two weeks. In the second, fourth, sixth week of the therapy, the plasma levels of theophylline after taking protheo for 4 hours and 12 hours were both within the effective concentration. CONCLUSION: Protheo is a kind of medicine with good effect, steady plasma concentration and less side effect for the patients with chronic asthmatic bronchitis.

Asthma↗

PubMed1997

[Surgical treatment in pulmonary tuberculosis with sputum long-term Mycobacterium tuberculosis positive].

OBJECTIVE: To evaluate the importance of surgical treatment in pulmonary tuberculosis with sputum long-term Mycobacterium tuberculosis positive. METHOD: Sixty-five patients with pulmonary tuberculosis who failed antituberculosis chemotherapy received lung resection or thoracoplasty. RESULT: Sputum negative conversion was found in sixty-three patients without recurrence during 1-3 year follow-up, while sputum positive conversion was found only in one case during the period of follow-up. Among all the patients, one died of respiratory failure after the operation, three complicated with empyema and recovered from operating again. CONCLUSION: Surgical operation is still important for treating pulmonary tuberculosis patients with sputum long-term Mycobacterium tuberculosis positive.

Adolescent↗

PubMed1997

[The role of platelet activating factor in pathogenesis of acute pancreatitis in dogs].

17 Beagle's dogs were divided randomly into tree groups: pancreatitis group (PG, n = 6), pan+BN52021 group (BNG, n = 6), control group (CG, n = 5). The acute pancreatitic model of PG and BNG was established by injecting sodium taurocholate and trypsin into the main pancreatic duct. Animals of BNG were injected PAF receptor antagonist BN52021 (5 ml/kg) intravenously 5 minutes and 3 hours respectively after acute pancreatitis induction. Blood amylase activity was determined by Winslow's method. PAF in blood and pancreatic tissue was determined by the platelet accumulation method. Blood amylase activity of PG increased by 466.7 +/- 111.6 than the baseline at 8 hours and increased significantly than that of BNG and CG (P < 0.05). Blood PAF level of PG increased from 30 minutes to 11.81 +/- 0.78 ng/ml at 8 hours. BN52021 inhibited very significantly the increase of PAF level (P < 0.01). PAF level in pancreatic tissue of PG was significantly higher than that of BNG and CG (P < 0.01). PAF may play an important role in early acute pancreatitis.

Acute Disease↗

PubMed1997

[Gonioscopy after foldable lens implantation through a sutureless tunnel incision].

OBJECTIVE: To investigate the changes of internal incision and the peripheral iris after cataract surgery through a sutureless tunnel incision. METHOD: Gonioscopy was performed on 58 eyes of 56 cases having undergone phacoemulsification and foldable intraocular lens implantation through a 3.5 mm sutureless tunnel incision. RESULTS: Gonioscopic findings showed that the internal incision was located in clear cornea anterior to Schwalbe's line in 56 eyes and just at the line in 2 eyes. Forty-two eyes (72.41%) had a smooth linear internal incision. There were small tags or curls in 14 eyes (24.14%). The Descemet's membrane detachments were detected in 5 eyes (8.62%) and peripheral iris anterior synechiae in 2 eyes (3.45%). CONCLUSION: The results suggest that the incidence of peripheral iris anterior synechiae be relatively low, however Descemet's membrane detachments easily occur in foldable lens implantation through sutureless tunnel incisions.

Aged↗

PubMed1997

[Posterior continuous curvilinear capsulorhexis in congenital cataract with opaque posterior capsule].

OBJECTIVE: To study the management of the opacified posterior lens capsule in congenital cataract extraction with intraocular lens (IOL) implantation. METHODS: Posterior continuous curvilinear capsulorhexis was performed on 36 eyes of congenital cataract with opaque posterior capsule. After the removal of cortical material and implantation of IOL, a perforation was made near the center of the posterior capsule through the equator of IOL, Healon was directed toward the opening and injected into the interspace between the posterior capsule and the anterior limiting membrane of the vitreous, and a 4 mm diameter posterior capsulorhexis was performed with a forceps. RESULTS: All the eyes achieved a clear visual axis. There was vitreous prolapse in two eyes. IOL decentration did not occur in any case. CONCLUSION: Posterior continuous capsulorhexis could remain the integrity of the peripheral capsular bag with a clear visual axis in the eye with congenital cataract and an opaque posterior capsule.

Adolescent↗

PubMed1997

[Clinical observation of foldable intraocular lens implantation by a small incision].

OBJECTIVE: To investigate a method of cataract operation capable of recovering normal visual acuity rapidly at early postoperative stage. METHODS: 131 eyes with senile cataract were divided into 3 groups: phacoemulsification with silicone foldable intraocular lens (IOL) implantation, phacoemulsification with one-piece IOL implantation and extracapsular cataract extraction with one-piece IOL implantation groups; the incision was 3.5 mm sutureless, 6.5 mm scleral tunnel incision and 11 mm limbal incision, respectively. RESULTS: 3 days after the operations, the average uncorrected visual acuity was 1.04, 0.51 and 0.46 and the proportion of normal visual acuity was 62.75%, 12.50% and 8.93% separately in the 3 groups. The average visual acuity and the proportion of normal visual acuity in 3.5 mm incision group was significantly higher than that of the other groups (P < 0.05). CONCLUSION: The phacoemulsification with foldable IOL implantation through a 3.5 mm sutureless incision can make good visual acuity rehabilitation at early postoperative stage.

Aged↗

PubMed1997

[Detection of herpes simplex viral genome in normal cornea].

OBJECTIVE: To determine whether herpes simplex virus (HSV) is present in normal cornea. METHODS: HSV genome in 22 corneas from donors without the history of herpes simplex keratitis (HSK), 6 positive control corneas from patients with active herpetic keratitis and 6 negative control corneas from embryos was detected by using polymerase chain reaction (PCR). RESULTS: HSV DNA was present in 20 of 22 normal corneas, and no viral genome was detected in any of the embryonic corneas. All the corneas from patients with active herpetic keratitis showed positive PCR results. CONCLUSIONS: The results indicate that the HSV genome is present in some normal corneas, that is concordant with the seropositive rate of HSV in people.

Adult↗

PubMed1997

[The development of a scale of life quality for diseases with visual impairment].

OBJECTIVE: To describe the development of a scale of life (QOL) quality which measures the quality of life of Chinese patients with visual impairment. METHODS: 20 items were chosen to form a scale. 57 cataractous patients with visual impairment and 60 glaucomatous patients with visual impairment and visual field loss at different levels simultaneously were measured by the scale to evaluate the validity, reliability and responsiveness of the scale. RESULTS: The scale consists of 20 items which cover four domains of QOL. The criterion related to the validity of the scale: r = 0.6865 (P = 0.000). The test-retest reliability of the scale: r = 0.8959 (P = 0.000). Coronback's alpha was 0.9358. The variance ratio (VR) of intra-individual variance to inter-individual variance was 0.0551 for overall scores. This correlation coefficient of split-half method was 0.9553. The responsiveness: t-test, t = 5.95 (P = 0.000). The effect size statistic was 1.533. CONCLUSION: The results also demonstrate that the scale has a satisfactory validity, reliability and responsiveness. It is suggested that the scale can be applied in clinical trails.

Cataract↗

PubMed1997

[Secondary foldable intraocular lens implantation].

OBJECTIVE: To investigate the effectiveness and technique of secondary foldable intraocular lens implantation. METHODS: The silicone intraocular lens was implanted secondarily in 25 aphakic eyes with intact posterior capsule and zonules or capsule rupture at central zone through a 3.5 mm sutureless scleral tunnel incision. RESULTS: 21 of the eyes (84%) achieved a uncorrected visual acuity equal to 0.5 or better and 9 eyes (36%) to 1.0 or better 1 day after the operation. All eyes achieved a postoperative corrected visual acuity equal to or better than that of the corrected visual acuity before the surgery in the follow-up periods. CONCLUSION: Secondary foldable intraocular lens is the most simple and effective method to correct aphakia with intact or almost complete posterior capsule.

Adolescent↗

PubMed1997

[Experimental study and clinical use of the dorsal digital flag flap].

To investigate the survival mechanism of the dorsal digital flag flap, an animal experiment was undertaken in a monkey. Eight dorsal digital flag flaps were created and examined by clinical observations, laser Doppler and biopsy. Of them six flaps were proximal-based and two were distal-based. The results showed: 1. The flag flaps survived without purplish discoloration or blister formation when the length-width ratio reached 10:1; 2. The perfusion unit measured by laser Doppler flowmetry decreased on the second postoperative day and began to increase from the third day, and returned to or surpass the preoperative level on the fifth day; 3. Biopsies confirmed that there was no specific artery in the pedicle of the flag flap but a rich dorsal digital venous network. The results indicated that the flag flap is a venous flap. Its arterial perfusion passed through the perivenous vascular network of the pedicle while the venous drainage is through the dorsal digital veins. Based on this study, 20 venous dorsal digital flag flaps have been used for reconstruction of digital defects associated with exposure of bone or tendon in 12 patients. Satisfactory results were obtained in all cases.

Adolescent↗

PubMed1997

[Effects of endogenous beta-adrenoceptor agonist on lung edema in scalded rats].

The effect of endogenous beta-adrenoceptor agonist on lung edema in scalded rats were investigated. The results showed that lung water content, wet/dry lung weight ration, bronchoalveolar lavage (BAL) protein concentration, malondialdehyde (MDA) content and leukocyte count in scalded and PPN treated rats were obviously higher than hose in control rats. The above parameters in scalded rats pretreated with PPN were even much higher than those of control and tended to be higher than burn group. It is suggested that endogenous beta-agonist is effective in the prevention of lung edema and lipid peroxidation, and it acts as an important anti-inflammatory factor in vivo.

Adrenergic beta-Agonists↗

PubMed1997

[Applied anatomy of the vessels in the dorsum of the finger].

Twenty adult cadavers were used to study the vessels of the flag flap in the dorsum of the finger with careful dissection and measurement. The results indicate: 1. The main blood supply to the dorsum of the finger comes from the cutaneous branches of the proper palmar digital artery. The dorsal digital artery is short and small, taking part in the blood supply of the dorsal digital skin of the proximal phalanges. 2. The veins communicate with each other, forming the first, the second and the third venous arch at the level of the distal phalangeal joint, the proximal phalangeal joint and the middle of the proximal phalanges. 3. The third venous arch is big. Proximally, the veins grouped to form radial and ulnar trunks, which communicate with the dorsum veins. 4. There are almost no veins in the area between the radial and ulnar venous trunk, which might be called the "vein lacking area." 5. The venous trunk, radial or ulnar, locates 4.1 +/- 0.1 mm from the midline of the finger dorsum at the middle of the proximal phalanges, and 5.2 +/- 0.2 mm at the metacarpophalangeal joint. Based on the results, we described the anatomical characteristics, the principle of design and the survival mechanism of the flag flap.

Adult↗

PubMed1997

[Study on the effect of continuous administration of IL-1ra in BXSB mice].

OBJECTIVE: To explore the consequences of IL-1 blocking in BXSB mice which is an experimental model for human SLE. METHODS: rh IL-1ra was expressed in E. coli and injected in BXSB mice. 13 of 4.5 month-age male BXSB mice were divided into two groups, one group was injected rh IL-1ra 10 times (twice a week) at dose of 400 micrograms per mouse each time, another group was injected PBS at the same time as control. We monitored serum ANA and proteinuria weekly, and detected IgG, C3 deposition and IL-6 expression in kidneys at 40th day. RESULTS: The results showed that the increased level of serum ANA and proteinuria in treatment group were not higher than in control group, the IgG, C3 deposition and IL-6 expression in kidneys and IL-6 activity in serum of the treaed group were lower than the control group, whereas no difference of GPT level in the two groups. CONCLUSION: IL-1 might play a pathogenic role in BXSB mice. Blocking or reducing IL-1 secretion would be of beneficial to the treatment of SLE.

Animals↗

PubMed1997

[Long-term results in 31 patients on total hemispherectomy modified for infantile hemiplegic epilepsy].

To overcome long-term complications of hemispherectomy, We modified its operative method. The modified hemispherectomy was performed for 31 patients between 1985 and 1992. These patients were studied for 7 years after operation. The results showed total control of seizures in 28 children with chronic epilepsy (90%) or near-total control in the others. No deaths or delayed complications were noted but improvement in behavior and hemiplegia. CT and MRI showed marked shift of the remaining hemisphere. On brain-stem auditory evoked potentials, the latency of peak I was not variant (P > 0.05). The method makes the insulation of the subdural cavity from the ventricular system more reliable, and climates the pathological conditions.

Adolescent↗

PubMed1997

[Nucleolar organizer regions enumeration in testicular teratoma].

To differenciate the benign and malignant testis teratoma. We investigated the value of AgNORs enumeration in 10 normal testis tissues, 6 benign teratomas and 7 malignant teratomas. The shape of the AgNORs in normal and benign teratoma was round, regular, with clear boundary and even size, setting in the center or the margin of the nucleus. In the malignant teratoma, the shape was irregular with unclear boundary and uneven size, setting in the near center or scattered in the nucleus. AgNORs enumeration: normal group: 1.56 +/- 0.17, benign teratoma: 2.40 +/- 0.26, malignant teratoma: 5.24 +/- 0.36. There was significant difference between the two groups (P < 0.01). The range of AgNORs enumeration in benign and malignant teratoma was separate. These results indicate that the mathod is helpful in differentiating benign and malignant testicular teratoma, and may provide an objective parameter.

Diagnosis, Differential↗

PubMed1997

[Characterication of reference strains of L. interrogans in China by ISSP-PCR].

The gene polymorphism of the DNAs extracted from reference strains of L. interrogans in China was characterized by LSSP-PCR primered by G1 or G2, which were a pair of specific primers for L. interrogans. The fingerprinting produced by LSSP-PCR showed that serovar lai, serovar canicola, serovar pyrogens, serovar autumnalis, serovar australis, serovar linhai, serovar wolffi and serovar haemolytic have similar patterns, but serovar hebdomadis, serovar javanica, serovar ballum, serovar pomona, serovar spaidjin, serovar tarassov and serovar manahao I have different profiles. This result agreed with the classification of genetic species by Yasuda. The utilization of LSSP-PCR banding patterns in the identification of leptospries in blood samples also gained encouraging results. Compared with the routine methods in genetic species classification, LSSP-PCR has the advantages of rapidity, simplicity and low-cost. It appears to be a promising tool in studying such slowly growing bacteria as leptospires. Further exploration of LSSP-PCR in classification and identification of Leptospires is worthwhile.

DNA, Bacterial↗

PubMed1997

[Immunogenecity of expressed protein p68 from recombinant plasmid rpDJt in L. interrogans serovar lai].

There are two types of infection caused by pathogenic microorganisms, intracellular infection and intercellular infection. Infection of pathogenic leptospira is an intercellular infection. The immunological reaction of host to intercellular infection is unique. The potential immunogen of an expressed protein should meet three criteria: it can be degraded (by antigen-present cells in the host); it should have antigenic epitope which can be recognized by specific antibodies and have at least one epitope that can be recognized by an MHC II protein and T cell receptor. In this study we report the cloning of an L. interrogans protein in plasmid rpDJt and the immunogencity of the expressed protein derivative. A genomic library of L. interrogans serovar lai strain 017 was constructed with the plasmid vector pUC18. Recombinant plasmids, designated pDJH2 and pDJ8 were screened from the bank. EcoRI-inserted fragment of 1. 9 kb recombinant DNA of pDJH2 was ligated into T7 RNA polymerase/promoter vectors (pT7-7). Then they were transformed into E. coli JM109 (De3), one of subclones, designated rpDJt was achieved. SDS-PAGE showed that the molecular weights of expression proteins were 68 kd and 23 kd respectively, designated p68 and p23. Purifying and isolating p68 and p23, we separated them from SDS-Polyacrylamide gels by using Side-Strip method. After fragmenting and electroeluting, p68 and p23 were injected into guinea pigs and rabbits. An extremely strong immune response to p68 was obtained since an anti-p68 antibody response could be detected to a dilution 1:524,288 (guinea pigs) and 1:262,144 (rabbits) by ELISA while anti-P23 antibody being 1:1024 (the same to guinea pigs and rabbits). The results of improved MTT and conA 3HTdR transformation methods showed the activities and proliferation of Th-cells were increased in guinea pigs after p68 immunization (IL-6, 83.25 IU/ml, IL-2, 28.75 IU/ml; RPI, 2.04, SI, 65.62%) Thlymphocyte existed in two subclasses, the Th1- and Th2-cells. A major role of Th2-cells is to "help" B-cells differentiate, replicate, and secrete antibody. The properties of these interactions explain why p68 makes good antigen and p23 does not. The antigens responsible for eliciting the production of protective antibodies are not known; however, several outer membrane proteins on L. interrogans are candidates for vaccine. Our results suggest that expresion protein p68 from recombinants (rpDJt) may be a candidate for gene engineered subunit vaccine for Leptospirosis.

Animals↗