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Biomedical subjects

S Li

Publications and source records attributed to S Li.

At least 775 records · Page 43Linked to original sources
PubMed1999

[Dynamic changes in IL-4, IL-5 and IL-10 in liver and bone marrow of mice infected with Schistosoma japonicum].

AIM: To observe the changes in Th2 cytokines in the liver and bone marrow of mice infected with schistosomiasis japonica. METHODS: ABC immunohistochemical staining technique and multimedia pathological picture analysis were used. Meanwhile, IL-4, IL-5 and IL-10 derived from livers and bone marrows of infected mice were observed at wk 8, 10 and 12 after infection. RESULTS AND CONCLUSION: In the livers of infected mice, the levels of IL-4, IL-5 and IL-10 increased obviously with a prolongation of the infection duration, IL-4 being the highest. However, in the bone marrow of infected mice, IL-4 level increased slowly with the duration of the infection, being lower than those in the liver at wk 10 and 12 after infection. The level of IL-5 was higher than that in the liver within 12 wk. At wk 10 after infection IL-10 tended to increase, but decreased thereafter and was obviously lower than that in the liver. In mice infected with Schistosoma japonicum, the immune response occurred mainly in the liver. IL-5 in the bone marrow showed auto-secretion.

Animals↗
PubMed1999

[The protective effect against Toxoplasma infection in mice immunized with laser-irradiated Toxoplasma tachyzoites].

AIM: To observe the level of immune responses and protective immunity in mice induced by laser-irradiated Toxoplasma tachyzoites (LIT). METHODS: ICR mice were immunized with LIT. After one month, the mice were challenged with live Toxoplasma tachyzoites to observe the effect of LIT. Indirect immunofluorescence assay and ELISA were used to detect CD4+/CD8+ lymphocyte subpopulation and IgG antibody. RESULTS: Immunization with LIT could partially protect the mice from Toxoplasma infection, prolong the survival time, enhance the CD4+/CD8+ ratio and produce specific IgG antibody. CONCLUSION: Intraperitoneal injection with LIT can induce partial protective immunity and specifically elicit ICR mice to generate humoral and cell-mediated immune responses.

Animals↗
PubMed1999

[Field application of oral artesunate for preventing Schistosoma japonicum infection].

AIM: To assess the preventive effect of oral artesunate against S. japonicum infection. METHODS: Residents in two pilots in the schistosomiasis endemic regions, 562 cases in Yanghe pilot, Wangjiang County and 218 cases in Shashan pilot, Guichi City, Anhui Province, were selected for this study. The residents were divided into two groups. Group I received artesunate 6 mg/kg once every 2 weeks for 4 times 2 wk after contacting with infested water from July to September in 1997. Group II received the same dosage of placebo at the corresponding times. Four weeks after the last administration, stool examination using hatching method and Kato's method was conducted to evaluate the effect. RESULTS: In Yanghe pilot, 2 cases were hatching positive in the artesunate-treated group with an infection rate of 0.7% (2/273), while 11 cases were stool positive in placebo group with an infection rate of 3.8% (11/289) and a mean EPG of 26.40 +/- 1.49. In Shashan pilot, all cases in artesunate-treated group were stool negative, while 7 cases were stool positive in the placebo group with an infection rate of 6.3% (7/111), and a mean EPG of 14.23 +/- 2.14. The protection rate of artesunate was 80.9% and 100%, respectively, in the two pilots. CONCLUSION: Artesunate can protect the residents from S. japonicum infection effectively.

Administration, Oral↗
PubMed1999

[The histopathological observation and the clinical significance of invasion of pyriform sinus apex in pyriform sinus carcinoma].

OBJECTIVE: To approach the clinical significance of pyriform sinus carcinoma invading to pyriform sinus apex. METHOD: Forty-three cases of surgical specimens from total laryngectomy and partial hypopharyngectomy of pyriform sinus carcinoma were made with the organ subserial sectioning and pathological study. RESULT: Pyriform sinus apex is the inferior 1/3 region of pyriform sinus. If pyriform sinus carcinoma invades its apex, the cancerous tissue easely infiltrated the adjacent structure and organs. CONCLUSION: The invasion of pyriform sinus apex is the beginning position and the most important sign of invading downward the tissue and surrounding organs in the inferior position of hypopharynx. When surgery treatment is performed, the total laryngectomy and hypopharyngectomy of ill hypopharynx is appropriate and necessary.

Adult↗
PubMed1999

[Identification study on pig's hoofnail].

The identification characteristics of pig's hoofnail on properties and tissue slice was initially probed from the viewpoint of identification in this paper in order to make basis for the systematic research on replacing Squama manitis.

Animals↗
PubMed1999

[Effects of the water-soluble extracts from the single herb of ganduqing against hepatitis B virus in vitro].

Comparing with Ara-Amp, the effects of the water-soluble extracts from the single herb of the formula for Ganduqing on HBeAg and HBsAg expression in 2.2.15 cells were studied. The results showed that the extracts of Serissa serissoides (DC) Druce, Hibiseus mutabilis Linn, Paedeuia scangens (Lour) Merr var tomentosa (BL) Hand-Mazz, Plumbago zeylanica L, Garcinia oblougifolia Champ and Begpnia edulia Levl had marked inhibition effects on HBeAg and HBsAg which expressed by 2.2.15 cells.

Antiviral Agents↗
PubMed1999

[Study on cultivation of Dendrobium flexicaule by habitat imitation].

Dendrobium drug, a rare medicinal herb, reproduces differcut for growth environment to lead rare resources. By studying of cultivation for many many years in Fuliu mountain area, authors had found out the cultivated method simulating the habitat of wild Dendrobium flexicaule. The results could supply a basis for its development and utilization.

Conservation of Natural Resources↗
PubMed1999

Distribution of human lens crystallins and their sulphydryl contents of different age in two-dimension electrophoresis.

PURPOSE: To analyze water-soluble (WS) human lens proteins of fetus, adult and age-related cataract by two-dimensional IEF/SDS-PAGE electrophoresis. METHODS: DACM [N-(7-Dimethylamino-4-methyl-3-coumarinyl) maleimide] was used to determine the lens proteins sulphydryl (SH) content. RESULT: Protein SH contents in WS lens proteins have no significant difference among fetus, adult and age-related cataract lens. This is different from the relative published results obtained in lens proteins of animal cataract model using similar SH detecting methods. CONCLUSIONS: IEF/SDS-PAGE electrophoresis demonstrated that there were much more fragmentation of crystallins during lens development and cataractogenic process. It is suggested that this phenomenon is likely to be due to further conformational changes in the fragmented cyrstallins during aging and cataractogenic process.

Adult↗
PubMed1999

Preliminary observation of one-site combined trabeculectomy and phacoemulsification in the management of cataract patients with drug-controllable glaucoma.

PURPOSE: To evaluate the effect of one-site combined phacoemulsification and trabeculectomy in the management of cataract patients with drug-controllable glaucoma. METHODS: Twenty-four cases (25 eyes) of cataract patients with glaucoma whose intraocular pressure could be controlled by drugs were observed. All had undergone one-site combined phacoemulsification and trabeculectomy. Intraocular pressure and visual acuity were observed. RESULTS: The follow-up was 1-21 months. Postoperative visual acuity was 0.4-0.6 in 9 eyes, 0.7-0.9 in 7 eyes and 1.0 or better in 9 eyes. Postoperative intraocular pressure was 2.17 +/- 0.764 kPa (16.28 +/- 5.73 mmHg), 1.83 +/- 0.90 kPa (13.74 +/- 6.77 mmHg) lower than that before surgery (t = 11.89, P < 0.01). Only one eye needed one drug to control intraocular pressure after surgery. CONCLUSIONS: One-site combined phacoemulsification and trabeculectomy is a safe, effective and convenient technique for the management of cataract patients with drug-controllable glaucoma.

Aged↗
PubMed1999

Effect of liposome-encapsulated total alkaloid of harmaline on rabbit lens epithelial cells: experimental study on the prevention of posterior capsule opacification.

PURPOSE: To investigate whether liposome encapsulated total alkaloid of Harmaline (TAH) as a therapeutic agent is beneficial to prevention of posterior capsular opacification (PCO). METHODS: Liposome-encapsulated TAH was prepared by modified freeze-thawing method. 0.1 ml of liposome-encapsulated TAH (0.2 mg/ml) was injected into the capsular bag during extracapsular lens extraction (ECLE) of each eye in total 10 rabbit eyes. Blank liposome or balance salt solution (BSS) was used as control. Slit-lamp examination and histopathological examination was used to evaluated capsule opacification. Intraocular pressure (IOP), density and morphology of corneal endothelia cells, the amplitude and latency of b wave of ERG were measured. RESULTS: The inflammatory response was mild both in TAH treated and the control group. PCO formation occurred in the control group 2 weeks postoperatively, but the posterior capsule was clear in TAH treated eyes. 4 weeks and 8 weeks after operation, PCO occurred both in TAH treated and control eyes. However, it was milder in the TAH treated eyes. IOP remained at the normal level in all eyes. There was no difference in the density of corneal endothelial cells, and the amplitude and latency of b wave of ERG between TAH treated and control eyes. Histopathological study revealed that lens epithelial proliferation occurred 4 weeks and the Soemmerings ring developed 8 weeks postoperatively in the control eyes. However, only mild vacuolization and pkynotic changes of lens epithelial cells were found in TAH treated eyes. Transmmission electronic microscopy demonstrated that there were mild cytoplasm vacuolization and mitochondria swelling of lens epithelial cells in TAH treated eyes. CONCLUSION: The results suggest that liposome encapsulated TAH can inhibit metaplasia and proliferation of lens epithelial cells in the rabbit eye without obvious toxicity to the eye tissue. It may be used as a potential agent to prevent the development of PCO.

Alkaloids↗
PubMed1999

[Genotype-phenotype correlation in Chinese patients with retinitis pigmentosa due to rhodopsin mutation].

PURPOSE: To investigate the genotype-phenotype correlation in Chinese patients with retinitis pigmentosa caused by rhodopsin gene mutation. METHODS: On the basis of the onset of symptoms, degree of morphological changes and progression of visual disability in three (3/83) patients with identified mutations, the correlation of the phenotype with the corresponding mutations was assessed. RESULTS: There was a certain degree of allele-specificity. Severe form of retinitis pigmentosa was found in patients with mutation in the cytoplasmic domain and mild form of retinitis pigmentosa in patients with mutation in the intradiscal domain. CONCLUSION: Although there is a certain relation between the mutant rhodopsin and ocular manifestation, we need to accumulate more materials before relating a rhodopsin mutation to a specific phenotype.

Adult↗
PubMed1999

[The effect of erycibele alkaloid on intracellular Ca2+ mobilation of cultured human ciliary muscle cells].

PURPOSE: To study the effect of erycibele alkaloid on cultured human ciliary muscle intracellular Ca2+ movement. METHODS: Intracellular Ca2+ was studied by Fura-2 probe and muscarinic antangonist was applied. RESULTS: Erycibele alkaloid had no any possible disturbulance on the examination system. The average resting[Ca2+]i was 79.3 +/- 28.7 nmol/Lol/L (n = 24). Erycibele alkaloid caused biphase [Ca2+]i elevation. Non-selective muscarinic antagonist Atropine (1 nmol/L) could completely block the 1 mumol/L erycibele alkaloid induced [Ca2+]i elevation; M1 antagonist Pirenzipine could not block the 1 mumol/L erycibele alkaloid induced [Ca2+]i elevation in low concentration (0.03 nmol/L) but in higher concentration (1 nmol/L). M3 antagonist 4-diphenyl acetoxy N-methy piperidine methiodide could completely block the 1 mumol/L erycibele alkaloid induced [Ca2+]i elevation in low and high concentration. CONCLUSION: The effect of erycibele alkaloid on increasing cultured human ciliary muscle cell intracellular Ca2+ is mediated by M3 receptor subtype.

Biological Transport, Active↗
PubMed1999

[The T cell activation from peripheral blood of patients with herpetic keratitis following in vitro activation].

OBJECTIVE: To evaluate the T cells activation from peripherial blood(PB) of patients with herpes simplex keratitis following in vitro activation. METHODS: The flow cytometry was used to investigate the expression of HLA-DR, CD25, CD69, CD71 on T cell from PB, the PB in culture, the PB cultured with phytohemagglutinin(PHA) or phorbol 12, 13-dibutyrate(PDB) or PDB and ionophore (IONO), in 7 cases with herpetic stromal keratitis (HSK) and 4 cases with herpetic ulcer keratitis(HUK). 6 normal people were set as control. RESULTS: The percentages of CD3+DR71+ was higher, and the CD3+CD71+ was lower from PB of patients with HSK than those from normal people. After the PB was cultured for 72 hours, the percentages of CD8+CD71+ in lymphocytes from HSK and CD8+DR+, CD8+CD69+, CD8+CD71+ in lymphocytes from HUK were lower than those from nomal people. And the CD8+CD71+ cells from HUK also was lower than those from HSK. When the PB was cultured with PHA for 72 hours, the percentages of CD3+CD71+, CD8+CD69+, CD8+CD71+ from HSK and HUK were lower than those from normal people. The percentages of CD8+CD25+ in lymphocytes from HUK also was lower than those from normal, and the CD8+CD69+ cells in lymphocytes was lower than those from HSK. The percentages of CD3+CD71+ in PB cultured with PDB for 72 hours, CD3+CD69+ in PB cultured with PDB and IONO for 72 hours from HSK were lower than those from normal people. CONCLUSION: The ability of T cells activation decreases in cases with HSK following in vitro activation. The lymphocytes in patients with HUK shows much lower activation than those cases with HUK.

Antigens, CD↗
PubMed1999

[Application of the treatment combined Western and traditional Chinese medicine in idiopathic uveo-encephalitis].

PURPOSE: TO study the factors, which influence idiopathic uveo-encephalitis(IUE) in the occurrence, diagnosis, treatment and prognosis. METHODS: Analysing retrospectively 33 cases of IUE(66 eyes) treated with Combined Western and Traditional Chinese Medicine(CWTCM). The etiology, diagnosis and prognosis were also be discussed. RESULTS: Of the 33 cases, there were 21 cases having the history of eating Xin, Hot, fried food or certain special protein. 15 cases were misdiagnosed. With the therapy of CWTCM, 15 cases recovered completely, 12 cases improved in visual acuity and 6 cases showed no improvement or deterioration. CONCLUSION: Xin, Hot, fried food and certain special protein may interfere with the human immunity, which may be the cause of the IUE in certain patients. Fundus fluorescein angiography is helpful in early diagnosis, the method of CWTCM is a efficient treatment, which values for further investigation.

Adolescent↗
PubMed1999

Differentiation of embryonic stem cells into neurons and retina-like structure in nude mice.

PURPOSE: To investigate the intraocular growth and biological characteristics of mice embryonic stem cells in nude mice. METHODS: Murine embryonic stem cells (D3 cell line) were cultured and maintained in an undifferentiated state in vitro, then transplanted into the anterior chamber of nude mice. Mophological and immunohistochemical examinations were implemented. RESULTS: Two to three days after transplantation, yellow-white floating granules, sheets and masses were seen inside the anterior chamber and vitreous cavity, and enlarged gradually. 14-20 days later, the mice were executed. Morphological examination showed that there were undifferentiated cells and some round or polygonal differentiated cells in anterior chamber and vitreous cavity. The morphology of these differentiated cells were similar to that of the retina. The cells were highly positive in NSE staining. CONCLUSION: The transplanted embryonic stem cells could grow in the eyes of nude mice with tendency to differentiate into neurons and retina-like structure.

Animals↗
PubMed1999

Differential gene expression in retina of myopic chicken eyes using mRNA differential display.

PURPOSE: To study differentially expressed genes in retina of experimental myopic chicken. METHODS: Experimental myopia in chicken was induced by form-deprivation. The mRNA in chicken retina was analyzed by using differential display. RESULTS: Experimental myopia was successfully induced in chicken through form-deprivation. Differentially expressed gene fragments were detected in retina of chicken with myopic development and recovery as compared with normal controls. CONCLUSION: The differential display of mRNA may be a useful way in cloning myopic-related genes.

Animals↗
PubMed1999

[The design and implement of PACS components in digital imaging apparatus].

The domestic application of PACS will surely be developed in the future, but it will still take a few years. This paper proposes a tradeoff scheme: Attaching to digital imaging apparatus the PACS components, which mainly contain the Auto-archiving unit, the Communication unit supporting WAN as well as LAN and the Converting Interface unit to the hospital PACS. In this way, a single digital imaging apparatus can archive and transmit data independently, also can merge with the hospital PACS conveniently in the future. In this paper, both the design and the implement are discussed in detail.

Angiography, Digital Subtraction↗