Biomedical subjects

S Li

Publications and source records attributed to S Li.

At least 541 records · Page 30Linked to original sources

PubMed2000

[Anomalous junction of pancreaticobiliary duct in congenital bile-duct cystic and cylindrical dilatation].

OBJECTIVE: To study the relationship between anomalous junction of pancreaticobiliary duct (APBD) and congenital bile-duct cystic as well as cylindrical dilatation. METHOD: Cholangiography was performed in 51 patients with bile-duct dilatation. RESULTS: APBD was diagnosed in 43 patients. In 8 patients (72%), the bile duct was drained into the pancreatic duct (B-P) in the cystic group, whereas in 14 patients (78%), the pancreatic duct was drained into the bile duct (P-B) in cylindrical group. There were significant differences in the internal diameter of the distal bile duct (P < 0.01), the maximal diameter of the pancreatic duct (P < 0.05) and the average angle of pancreaticobiliary junction (P < 0.01) in cystic and cylindrical groups. CONCLUSIONS: Different types of APBD after lead to different pathological changes of the biliary tract. The narrowed distal bile duct joins the pancreatic duct at a right angle in most cystic cases and the pancreatic duct joins the bile duct at an acute angle in most cylindrical cases.

Bile Duct Diseases↗

PubMed2000

[Measurement of gastric emptying with Xianshizi in patients with resected esophageal cancer].

OBJECTIVE: To determine gastric emptying radiological in patients with esophageal cancer and to obtain a similar effect produced by radionuclide study. METHODS: A new product-Xianshizi (XSZ) made of the mixture of barium and silica gel was used quantitatively to evaluate gastric emptying. 17 patients with resected esophageal cancer before and after operation, and 7 healthy volunteers were studied. RESULTS: Preoperative patients had obviously delayed gastric emptying compared with the control group (P < 0.01). Postoperative patients had more delayed gastric emptying compared with themselves (P < 0.05). CONCLUSIONS: Postoperative gastroparesis is related to delayed gastric emptying. The results of this study were identical with those obtained by scintigraphy. Compared with isotope studies, X-ray exam after taking meal composed of XSZ may eventually contribute to detecting delayed gastric emptying quantitatively.

Adult↗

PubMed2000

[Expression of thymidylate synthase gene and recurrence of colorectal carcinoma: their relation and clinical significance].

OBJECTIVE: To study the relationship between expression of thymidylate synthase (TS) gene and recurrence of colorectal carcinoma and its effect on clinical treatment. METHODS: RT-PCR was used to detect the expression of TS gene in primary foci, para-tumoral intestine mucosa, local recurrence, abdominal-pelvic dissemination and hepatic metastasis in 68 cases of colorectal carcinoma, and TS protein was examined with western blot. RESULTS: In 68 cases of colorectal carcinoma, the expression of TS gene in primary foci was 22.1% (15/68); and the positive rates of TS gene expression in local recurrence, abdominal-pelvic dissemination and hepatic metastasis were 88.5% (23/26), 85.0% (17/20), 40.9% (9/20) respectively. The rates of TS protein expression in primary foci, local recurrence, abdominal-pelvic dissemination and hepatic metastasis were 22.1% (15/68), 84.6% (22/26), 80.0% (16/20), 36.4% (8/22) respectively. The negative expression of TS gene and TS protein was detected in paratumoral intestinal mucosa. The results of TS gene and TS protein expression were identical with those the two methods. The positive rates of TS gene and TS protein expression in diversified recurrence foci and metastasis were higher than those in primary foci (P < 0.01). The differences of TS gene and TS protein expression rates between recurrence and hepatic metastasis were significant (P < 0.01). The expression rates of TS gene and TS protein in local recurrence and abdominal-pelvic dissemination tissues were higher than those in hepatic metastasis. CONCLUSION: Overexpression of TS gene plays an important role in the process of local recurrence and abdominal-pelvic dissemination of colorectal carcinoma.

Adult↗

PubMed2000

[Experimental study on herpes simplex virus-1 functional gene latency in cornea].

OBJECTIVE: To investigate the possible existence of herpes simplex virus-1 (HSV-1) functional gene latency in cornea. METHODS: A herpes simplex keratitis (HSK) model was established in New Zealand White (NZW) rabbit corneas, latently infected corneas were transplanted to one eye of each naive NZW rabbit. One week after the operation, the corneal buttons were removed and divided into three portions. One portion was processed for HSV-1 antigen detection, another was for HVS-1 latency associated transcript (LAT), thymidine kinase (TK) and DNA polymerase gene detections by polymerase chain reaction (PCR), and the last portion after tissue culture for 3 weeks, it was co-cultured with rabbit kidney cells (RK) for one week, then the expression of HSV-1 antigen in the RK cells and this portion of corneal button was detected. RESULT: In the 15 corneal samples negative for HSV-1 antigen, LAT gene was detected in 8 samples, TK gene in 10 and DNA polymerase gene in 6 samples. HSV-1 antigen was detected in 3 samples of RK cells and 6 cultured corneal samples. CONCLUSION: HSV-1 functional genes are retained in latently infected cornea.

Animals↗

PubMed2000

[A diagnostic analysis of imaging in ocular cysticercosis].

OBJECTIVE: To discuss B-ultrasonography, CT and MRI appearances and image diagnostic value for ocular cysticercosis. METHOD: In the 29 cases with ocular cysticercosis, 21 cases were examined by B-ultrasonography, 19 cases by X-ray computed tomography (CT) and 6 cases by magnetic resonance imaging (MRI). RESULTS: At the living stage of the cysticercus, B-ultrasound might detect parasitic cyst and its movement in the eyeball and orbit, extraocular muscle irregular hyperplasia; CT might detect parasitic cyst in the orbit and the pathologic extraocular muscle; MRI might detect the parasitic cyst in the eyeball and the orbit and the pathologic extraocular muscle. At the non-living stage of the cysticercus, B-ultrasonography and CT might discover calcification focus in the eyeball and extraocular muscle. CONCLUSION: Image appearances of ocular cysticercosis depend on the parasitic site and living status of cysticercus in the eye region. For diagnosis, B-ultrasonography may first be selected, MRI secondly selected for living stage and CT secondly selected for non-living stage of the ocular cysticercosis.

Adolescent↗

PubMed2000

[Secondary glaucoma after penetrating keratoplasty].

OBJECTIVE: To investigate the etiology and management of secondary glaucoma after penetrating keratoplasty (PKP). METHODS: Eighty cases (80 eyes) with secondary glaucoma occurring in 1,450 cases (1,500 eyes) of PKP, its causes, time of onset and the relationship with the primary disease were analyzed. According to different causes, they were treated with different therapies. RESULTS: The intraocular pressure (IOP) of 63 cases (63 eyes) became normal after drug treatment for 2 - 3 weeks. In 17 cases (17 eyes) who had received antiglaucomatous operations, 13 cases' (13 eyes) IOPs were normal and 4 cases' (4 eyes) still high. CONCLUSIONS: Accurate perioperative management can decrease the incidence of secondary glaucoma after PKP. The majority of secondary glaucoma can be controlled by different therapies according to different causes.

Adolescent↗

PubMed2000

[A comparison between latanoprost and timolol in treatment of patients with primary open-angle glaucoma and ocular hypertension].

OBJECTIVE: To evaluate the therapeutic value of latanoprost on glaucoma. METHODS: In an open-label fashion, multicenter, randomized control clinical trial, the efficacy and adverse drug reactions of topical application of 0.005% latanoprost once daily were compared with that of 0.5% timolol twice daily for 12 weeks in patients with open-angle glaucoma or ocular hypertension. RESULTS: The study included 128 patients (63 patients in latanoprost group and 65 patients in timolol group) and 117 patients remained at the end of the study (60 cases in latanoprost group and 57 cases in timolol group). Comparing 12 weeks with baseline diurnal intraocular pressure (IOP), the IOP reduction (mean +/- standard deviation) in latanoprost group was (7.5 +/- 0.3) mm Hg (1 mm Hg = 0.133 kPa) (32%, t = 22.73, P < 0.0001) greater than the reduction in timolol group (6.1 +/- 0.3) mm Hg (26%, t = 17.94, P < 0.0001), the difference between the two groups being significant (F = 9.54, P = 0.0026). Two patients treated with timolol and none treated with latanoprost were withdrawn from the study because of inadequate IOP control; 3 patients with latanoprost had foreign body sensation. In latanoprost group, there was one patient whose eyelashes became darker and longer at the last visit (the 12th week). No ocular and systemic adverse events related to the two drugs were found. CONCLUSION: It is demonstrated that 0.005% latanoprost topically applied once daily is well tolerated and more effective in reducing IOP than 0.5% timolol topically applied twice daily. Thus, latanoprost has the potential to be a new first-line antiglaucoma drug.

Adrenergic beta-Antagonists↗

PubMed2000

[A preliminary report of ocular surface disease treated by limbal transplantation].

OBJECTIVE: To evaluate the effect of ocular surface disease treated by limbal transplantation. METHODS: In a prospective study, 5l eyes of 50 patients were treated with three kinds of surgical techniques: first, conjunctival limbal autograft (CLAU) transplantation; second, corneal lamellar allograft combined with limbal autograft transplantation and third, corneal lamellar allograft combined with limbal allograft transplantation. Their effects were evaluated. RESULTS: All of the 18 patients of CLAU group were cured. The second surgical technique resulted in restoration of a stable ocular surface in 19 of 20 cases. 10 patients' visual acuities were improved two or more than two lines. 13 eyes of 12 patients were treated with the third surgical technique, resulting in graft ulceration in 4 patients and graft rejection in 4 patients with bad prognosis. CONCLUSIONS: The effect of unilateral ocular surface disease treated with limbal autograft transplantation is satisfactory. For bilateral ocular surface disease, the method of limbal allograft transplantation should be improved to resolve some problems such as graft rejection.

Adolescent↗

PubMed2000

Dietary fiber and type 2 diabetes.

This article addresses the current theory, research, and implications of dietary fiber in the management of type 2 diabetes mellitus (DM; non-insulin-dependent DM). Dietary fiber shows promise in the management of type 2 DM. The inclusion of sufficient dietary fiber in a meal flattens the postprandial glycemic and insulinemic excursions and favorably influences plasma lipid levels in patients with type 2 DM. Water-soluble fiber appears to have a greater potential to reduce postprandial blood glucose, insulin, and serum lipid levels than insoluble fiber. Viscosity of the dietary fiber is important; the greater the viscosity, the greater the effect. Possible mechanisms for metabolic improvements with dietary fiber include delay of glucose absorption, increase in hepatic extraction of insulin, increased insulin sensitivity at the cellular level, and binding of bile acids. Patients with type 2 DM should increase their dietary fiber intake to 20 to 35 g/d and be aware of the considerations when increasing fiber intake. The nurse practitioner is in an ideal position to promote dietary fiber intake in such patients.

Blood Glucose↗

PubMed2000

[Study on the protective effect of grape procyanidins on DNA damage induced by irradiation].

OBJECTIVE: To study the protective effect of grape procyanidins (GPC) on the irradiated DNA damage. METHOD: In vivo study: After orally administration of GPC, mice in different groups were exposed to whole-body (60)Co gamma-rays at a dose of 2.0 Gy and the fluorospectrometry method was used to rapidly detect the DNA strand breaks of mouse spleen cells. In vitro study: Human venous blood with different concentrations of GPC was exposed to (60)Co gamma-rays at a dose of 1.0 Gy, then WBC DNA content was measured after 1, 30 and 60 min exposure, respectively. RESULTS: In the three treated groups (500, 250 and 50 mg/kg of procyanidins), DNA content of mouse spleen cells was 18.76%, 15.74% and 5.06% more than that of the positive controls respectively. DNA content of human WBC was higher than the positive controls by 17.6%, 11.1% and 3.9% increase respectively one minute after irradiation and 21.3%, 13.8% and 5.3% increase respectively 60 min after irradiation. These differences were all statistically significant. CONCLUSION: GPC has a protective effect on DNA damage caused by irradiation, and is helpful to DNA repair.

Animals↗

PubMed2000

[Research on origin and development of "Zhang Yi Tie", a celebrated physician of Xin'an].

The "Xin' an Medicine", beginning from Jin - Tang and flourished in Ming - Qing periods, occupied a significant position in the history of TCM. A detailed textual and research on the origin and development of "Zhang Yi Tie" of Dingtan, She Country, Huizhou, a representative medical family of Xin' an clinical physician, is made here. Maintaining that the title of "Zhang Yi Tie" began in Jiajing reign of the Ming dynasty, was applied until now. In the period of over 450 years of 15 generations successive ages, Zhang was conversant with acute febrile diseases, intractable miscellaneous disease of internal medicine, with the "powdered medicine" inherited. The clinical characteristics of all generation of Zhang were explored, holding that regional medical schools, representative medical families are one of the important constructive forms in the developmental process of TCM.

China↗

PubMed2000

[Long-term expression of MHC-I gene in recipient mice].

OBJECTIVE: To explore the possibility of long-term expression of transferred MHC-I gene in recipient mice. METHODS: MHC-I gene of the donor mouse (C(57)BL/6) was transferred into the recipient (BALB/c) hematopoietic cells by retrovirus-mediated gene transfer technique, and the gene transferred cells were reinfused into the recipient mice. PCR, RT-PCR and FACS were used to assay the long-term expression of MHC-I gene in the recipient mice. RESULTS: The gene transferred mice hematopoietic cells and their G418-resistant CFU-GM were demonstrated to be able to express the MHC-I gene. The cells proliferation and differentiation had no change. The MHC-I gene could be expressed for a longer period in the recipient mice. CONCLUSION: This experiment makes ready for the further research on inducing transplantation tolerance through MHC-I gene transfer.

Animals↗

PubMed2000

[Clinical and experimental study on treatment of acute catarrhal otitis media with eryanling oral liquid].

OBJECTIVE: To study the clinical effect and mechanism of Eryanling (EYL) oral liquid in treating acute catarrhal otitis media (ACOM). METHODS: Sixty-eight cases (89 ears) of ACOM in the treated group were treated with EYL and compared with 34 cases (44 ears) in the control group treated with cephalexinum. Experimental study of effect of EYL on immune function in mice and non-suppurative otitis guinea pig was also conducted. RESULTS: The total effective rate of the treated group and the control group was 91.0% and 84.1% respectively, and their rate of curing 80.9% and 70.5% respectively, though the effect in the former was better, statistic analysis showed no significance between them. The effect initiated obviously earlier in the treated group than that in the control group. Results of experimental study suggested that EYL could strengthen the nonspecific immune function, cellular immune function and humoral immune function in mice, and reduce the degree of inflammatory exudation and mucosa swollen in guinea pig. CONCLUSION: EYL has good therapeutic effect in treating ACOM.

Acute Disease↗

PubMed2000

[Immune reconstitution in immunosuppressed pigs with human immune competent cells].

To reconstitute the immune system of the pig with human immune cells after destroying or suppressing it, so that the transplant immunoreactivity between human and pig could be monitored in vivo. Fifteen Chinese Neijiang pigs were divided into 3 groups. Group A (n = 5) received immunosuppression through intravenous infusion of methotrexate (MTX), myleran and cyclophosphamide (CTX). Group B received intraperitoneal injection of human bone marrow and spleen cells (HBSC) after the same treatment as group A. Group C (n = 5) received intravenous infusion of HBSC after the same treatment as group A. Blood routines and biochemical analysis were monitored. The chimerisms of human cells were supervised through immunohistochemistry of cytospin of peripheral blood from recipient pigs and flow cytometry of the same specimen and immunohistochemistry of frozen tissue sections and paraffin sections. All pigs in group A survived definitely. Group B and C survived 3 days to 54 days and 15 hours to 60 days respectively. The peripheral white blood cell count (WBCC) of group A, B and C decreased to the bottom 7 days after drug administration, about 1%-10% of the normal level. After transplantation, the WBCC of group B and C recovered more slowly than those of group A. Human cells were found in the recipient pigs of group B and C after transplantation through immunohistochemistry of peripheral blood cytospin and flow cytometry. Infiltration of human cells was found in the spleens and lungs of group C and D through immunohistochemistry. The chimerism of human cells in recipient pigs could be established by transplanting human bone marrow and spleen cells into immunosuppressed pigs. It is possible that XGVHD occurs in the recipient, which offers a novel animal model for studies on immunoreactivity of pig-to-human xenotransplantation. The proportions of human cells in peripheral blood of recipient pigs are low long after transplantation through both intraperitoneal injection and intravenous infusion.

Animals↗

PubMed2000

[Analysis for monobromo- and dibromo-pentaerythritol by gas chromatography].

A method for separation and determination of dibromo-pentaerythritol (DBPT), monobromo-pentaerythritol (MBPT), and tribromo-pentaerythritol (TBPT) by gas chromatography was developed. The conditions for determination were: BPX-70 capillary column, operated at 240 degrees C, with both FID detector and injector at 280 degrees C. Xylitol was the internal standard. Linear regression equations were YDBPT = 0.0423X-0.0124 (r = 0.9998) and YMBPT = 0.0634X-0.0835(r = 0.9966). This method is rapid, simple and accurate.

English Abstract↗

PubMed2000

[Culture of endotheliocytes derived from guinea-pig common carotid artery and establishment of guinea-pig donor model].

Since endotheliocyte strains of guinea-pig and inbred strains of guinea-pig are not easily obtained, it is impossible to ensure the consistence of antigenicity between endotheliocytes and donor organs when endotheliocytes are used as an inductor in the research of immunological tolerance. So it is necessary to establish the guinea-pig endotheliocyte-donor model. One side of common carotid arteries was removed and reversed. After collagenase digestion, culture of endotheliocytes was performed. And the survival time of guinea-pig was observed. Cultured cells were confirmed as endotheliocytes by the study of morphology, immunohistochemistry and electron microscopy. The two-month-survival of guinea-pigs was 92% (103/112). The establishment of guinea-pig endotheliocyte-donor model can provide the foundation for the research of guinea-pig endotheliocyte and immunological tolerance.

Animals↗

PubMed2000

[Clinical study on interferon treatment of chronic idiopathic thrombocytopenic purpura].

The efficacy and mechanism of interferon alpha-2a (IFN alpha-2a) were assessed in the treatment of chronic idiopathic thrombocytopenic purpura (cITP). 20 patients with cITP (treatment group) were treated with IFN alpha-2a 3MIU i.m. once a week for 8 weeks; 28 patients with cITP (control group) were treated with prednisone 1 mg/(kg.d) for 4 weeks. Blood platelet counts (BPC), megakaryocyte number, immunnological parameters, percent and absolute counts of reticulated platelets (RPs) and megakaryocyte colony formation units were observed before and after therapy. The results showed that the efficacy of IFN alpha-2a was better than that of corticosteroid, P < 0.05. The RPs% decreased and the platelet-producing megakaryocyte percentage increased from 11.43% to 33.19% significantly after IFN alpha-2a therapy, but there were no significant changes in immunnological parameters. These indicate that IFN alpha-2a is effective in treating cITP, the mechanism may be based on promoting the megakaryocyte development and activating the production of platelets.

Adolescent↗

PubMed2000

[Study on fermentation of 1,13-tridecanedioic acid by Candida tropicalis].

A mutant of Candida tropicalis SP-1, SP-UV-56, which can produce 1,13-tridecanedioic acid or 1,11-dicarboxylic acid (DCA13) 1.25 times as much as its parental strain SP-1, but hardly assimilates nalkane as carbon source for its growth, was obtained by ultraviolet treatments. A fermentation technology with a supplement of acetate at the latter middle of logarithmic growth was developed. Using sucrose as carbon source for its growth and supplying acetate the yield of DCA13 reached 153 g/L for 144 h incubation in a 13.7 L auto-controlled stirred tank, about 29.7% higher than that obtained in the broth without acetate. An effective technique that can maintain higher yield was provided by enhancing mixing of broth and controlling low DO level. With these techniques the DCA13 yield reached 172 g/L in a 20 m3 stirred tank, and produced 2.25 tons DCA13 in 15.0 m3 broth.

Alkanes↗