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Biomedical subjects

S Li

Publications and source records attributed to S Li.

At least 361 records · Page 20Linked to original sources
PubMed2001

[Umbilical cord blood transplantation (UCBT) in thalassemia children].

OBJECTIVE: To evaluate the efficacy of allo-UCBT in thalassemia children. METHODS: Five patients with beta-thalassemia major (genotypes were homozygote of beta41-42, beta654 and double heterozygosities of beta41-42/beta654, beta41-42/-28, beta654/-28, respectively) were treated by allo-UCBT. The median age was 3 year and ten month old (31/2 - 7 2/12). Four donor-recipient pairs were HLA-identical and one 1 locus mismatch. The patients received a median UCB nucleated cells (NC) of 5.5 x 10(7)/kg body weight [range (4.7 - 7.7) x 10(7)/kg] and CD(34)(+)CD(38)(-) cells 2.8 x 10(5)/kg [range (0.6 - 4.5) x 10(5)/kg] and CFU-GM 1.09 x 10(5)/kg [range (0.24 - 230.00) x 10(5)/kg]. The conditioning regimen consisted ofbusalphan 16 - 20 mg/kg, cyclophosphamide 180 - 200 mg/kg, horse antithymocyte globulin (ATG) 90 mg/kg or rabbit anti-lymphocyte globulin (ALG) 25 mg/kg and melphalan of 90 mg/m(2) was added to four cases. Cyclosporine A (CsA) alone was administered in 3 cases, and a combination of CsA and methotrexate in the other 2 cases. RESULTS: Four cases were engrafted, but one rejected and recurred thalassemia state at day 60 past UCBT. One case had autologous reconstitution. The reconstitutions were found in all patients with neutrophils 0.5 x 10(9)/L at day 19 (14 - 22), platelets > 20 x 10(9)/L at day 37 (21 - 63). Two cases developed grade I and II acute GVHD respectively. None developed chronic GVHD. Three patients survived disease-freely (survival with ex-thalassemia state). The median follow-up was 18 months, the probability of survival was 100%. CONCLUSION: Allo-UCBT could reconstitute hematopoiesis with low frequency and mild grade of GVHD. It is an effective treatment for thalassemia.

Child↗
PubMed2001

[Serum glutathione S-transferase in bromobenzene-induced acute hepato-toxicity in mice].

The activity of serum glutathione S-transferase (GST) was tested in bromobenzene-induced acute hepatotoxicity in mice. The data showed that a lot of small local necrosis in liver biopsy was observed and serum GST activity was increased after 2 h of treatment with bromobenzene (150 mg/kg i.p.) in mice. Serum alanine transaminase (ALT) activity was also gradually enhanced after 8 hours of treatment. The most obvious changes were at 16 hours of bromobenzene treatment, and serum GST activity was firstly restored to normal level after 24 hours of treatment. There were good positive correlations between serum GST, ALT, as well as serum GST and the changes on liver biopsy. Evaluating analysis of the diagnosis test showed that the sensitivity, specificity and accuracy was 89%, 75% and 82% in serum GST test, and 39%, 92% and 64% in serum ALT test respectively. The ROC work curve also demonstrated that serum GST was more sensitive and specific than serum ALT.

Alanine Transaminase↗
PubMed2001

[Effects of manganese, zircon and lithium alone on rat liver lipid peroxidation].

Lipid peroxide (LPO) in rat liver was detected by malondiadehyde (MDA) colorimetry. The effect of manganese, zircon and lithium alone on lipid peroxidation in rat liver was also studied. The results showed that manganese and zircon at the doses of (9.862-1.972) x 10(-4) and (0.1972-9.862) x 10(-5) nmol/L respectively decreased LPO in rat liver(P < 0.01). Lithium inhibited lipid peroxidation at the dose of (19.72-1.972) x 10(-4) nmol/L, and induced lipid peroxidation at higher concentration.

Animals↗
PubMed2001

[Study on an enhancing agent for removing arsenic from drinking water].

Drinking water contaminated by arsenic for an extended period of time could be detrimental to the health of people. Some preliminary symptoms could be alleviated by drinking water non-contaminated. It is important to develop an arsenic removal agent with a specific property of most efficient, cost-effective and easy for operation. The results showed that the capacity of the agent developed in this study was 10 times higher for arsenic removal than other agent available. The lowest arsenic content of the treated water was 0.05 mg/L. The special function of this agent was arsenic removing without changing other components and the concentrations of other elements in the treated water. The operation and management was simple without adjusting pH of the influent and effluent water. The agent was 5 times cheaper in cost than alumina or activated carbon, because it was a reusable oxidation-catalyst. Therefore, the agent could be widely applied in drinking water plants or used as a purifier at home in the high arsenic areas.

Arsenic↗
PubMed2001

[Studies on liver cancer induced by non-necrotizing dose of diethylnitrosamine in rats].

OBJECTIVE: To provide an appropriate animal model of liver cancer and sensitive indicator for carcinogen risk assessment. METHODS: The developmental processes of liver cancers in rats initiated by non-necrotizing dose of diethylnitrosamine and promoted by phenobarbital were studied. RESULTS: Most of the preneoplastic lesions and liver cancers were made up of eosinophilic cells. The placental glutathione S-transferase (P-GST) staining could allow a lot of positive single cells, foci and nodules to be seen. But the lesions showed by HE staining expressed higher proliferating cell nuclear antigen (PCNA) than those showed by P-GST staining expressed, indicating a greater possibility for these lesions to persist and develop further. The cellular morphologic analysis demonstrated that the ratio of nuclear radii to cytoplasm radii decreased in the cell in PCNA positive expressing nodules. In addition, the nodules showed by P-GST expressed higher PCNA than those the foci expressed, indicating an increased possibility to develop further in the nodules. CONCLUSION: PCNA expression may be used as a relatively ideal marker for predicting the potential development of precancerous lesions in the experimental model of rat liver cancer induction.

Animals↗
PubMed2001

[Purification of conjugated bilirubin from human urine].

OBJECTIVES: To develop a simple, practical method with fine yield and purity for separating conjugated bilirubin(Bc) from a sustainable and facile material in order that it could serve as a calibrator for total and direct bilirubin determination. METHODS: Isolating Bc from urine of patients with obstructive jaundice by CHCl3: CH3CH2OH(1:1, V/V) followed by CNBr activated Sepharose 4B-HAS affinity chromatography, we analyzed the products by the use of Doumas J-G diazo method and HPLC, respectively. RESULTS: Determined by J-G diazo method, the crude product extracted by CHCl3: CH3CH2OH(1:1, V/V) yields 90.2 +/- 5.4%(n = 3) of urine Bc, the ratio of Bc to total dry weight being 67.9 +/- 6.4%(n = 3). The final product purified by affinity chromatography with the ratio of separation of 1.5, 1.737 and analyzed by HPLC has the yield of 46.9% and purity of 94.7%(BDG of 76.2%, BMG of 18.5%). CONCLUSION: The above data demonstrate that urine conjugated bilirubin can be successfully purified by CHCl3: CH3CH2OH extraction followed by Sephrose-HSA affinity chromatography with sufficient purity and output.

Bilirubin↗
PubMed2001

[Clindamycin soluble suppository-production and its efficacy in treating bacterial vaginosis].

OBJECTIVE: To make the soluble suppository of clindamycin which is an effective drug for treatment of Bacterial vaginosis and to treat patients in clinical trials. METHODS: Outpatients who had vaginal infections from Sept. 1998 to Jan. 1999 were investigated. The diagnoses of bacterial vaginosis were made according to Amsel's criteria and 80 patients were included in this study. They were divided into two groups to be treated respectively by metronidanole suppository and clindamycin suppository which were made by our hospital pharmacy. RESULTS: The cure rate of metronidanole was 87.5% and that of clindamycin 90.3%; No significant difference was noted between the two groups(P > 0.05). The new clindamycin suppository was not irritant and it was easy to dissolve in vagina. There was no discontinued treatment because of side-effect. CONCLUSION: The clindamycin suppository does not irritate the mucosa of vagina, it is an efficacious remedy for bacterial vaginosis.

Administration, Intravaginal↗
PubMed2001

[Effects of fluid shear stress on PGE2 synthesis of primary osteoblast-like cells in rats].

OBJECTIVE: The purpose of this study is to study the function of PGE2 during the signal transduction in which fluid shear stress regulates the proliferation and differentiation of osteoblast-like cells. METHODS: The isolated rat primary osteoblast-like cells were exposed to fluid shear stress 0.12 mN/cm2 for 5, 10, 15, 30, 60 and 120 minutes respectively in a flow chamber. The release of PGE2 was examined. RESULTS: After exposure to fluid shear stress, the PGE2 synthesis of rat primary osteoblast-like cells increased significantly (P < 0.01) when compared with the control. After 10 minutes, the release of PGE2 began to increase significantly (P < 0.01) and the effect was maximal after 60 minutes (P < 0.01). CONCLUSION: PGE2 pathway may be one of the signal-transduction pathways which can transduce the fluid shear stress into osteoblast-like cells and then stimulate the bone remodeling.

Animals↗
PubMed2001

[A study on the relationship between deciduous tooth caries in pre-school children and their neonatal conditions].

OBJECTIVE: This study was designed to examine whether the neonatal conditIon might be related to the caries of deciduous teeth in pre-school children. METHODS: 1000 children from 3 years old to 6 years old in Chengdu were surveyed, and 260 children with dmft > or = 6 and 260 caries-free children were chosen. Data was collected by 200 pairs of useful questionnaire from their parents and analyzed by using logistic regression analysis and chi 2 test. RESULTS: The neonatal condition was closely related with incidence rates of deciduous caries in pre-school children (P < 0.05). The incidence rate of children with premature delivery was higher than that with full-term normal delivery (P < 0.05). No significant difference was showed in children with full-term normal delivery and children with dystrophy (P < 0.05). CONCLUSION: There should be emphasis on the children with premature delivery in caries preventive cares.

Child↗
PubMed2001

[A study on mRNA expression levels of integrin beta 1 during the healing process of mandibular fractures].

OBJECTIVE: The aim of this study was to investigate the spatial and temporal mRNA expression changes of integrin beta 1 during the healing process of mandibular fractures. METHODS: The in situ hybridization was performed using digoxigenin-labeled integrin beta 1 mRNA oligonucleotide probe. During the healing process of mandibular fractures, the staining pattern of beta 1 mRNA was examined both spatially and time-dependently. RESULTS: Integrin beta 1 was widely present in the cells of the fractured bone, especially in osteoblasts. The positive staining was observed in the cytoplasm and nucleolus of osteoblasts. After 7 days of fracture, the expression level of integrin beta 1 increased. The peak appeared between the 14th and the 30th day, and almost recovered to normal between the 60th and 90th day. CONCLUSION: During the healing process of mandibular fracture, the expression level of integrin beta 1 in bone, especially in osteoblasts rose. It is suggested that integrin beta 1 may play an important role in the fracture healing process.

Animals↗
PubMed2001

[Expression of bone morphogenetic protein (BMP)-2, 4 in mouse embryonic tooth during the bud stage].

OBJECTIVE: The purpose of this paper was to investigate the distributions of bone morphogenic protein (BMP)-2, 4 in the developing molars of mice during the bud stage and, the functional relationship between BMP-2 and BMP-4. METHODS: The distribution of BMP-2, 4 were examined in developing mouse molars on the 14th (the 14th embryonic day), using monoclonal antibodies and immunohistochemistry methods. RESULTS: BMP-2, 4 were detected both in the epithelial cells and dental mesenchyme cells. CONCLUSION: There is a similar distribution pattern between BMP-2 and BMP-4. Therefore, there may be a functional relationship between BMP-2 and BMP-4 during the development of mouse embryonic teeth in the bud stage.

Animals↗
PubMed2001

[Clinical comparison of bilateral neck dissection for supraglottic carcinoma].

OBJECTIVE: In order to rise the survival rate and quality of life, the patients with bilateral neck dissection was investigated in supraglottic carcinoma. METHOD: 76 cases were retrospectively analyzed. In terms of clinical diagnosis, 33 cases who had bilateral neck metastasis had received simultaneously bilateral neck dissection. 43 patients first diagnosed unilateral neck metastasis got subsequent bilateral neck dissection. All of the patients were pathologically diagnosed. RESULT: The 3 and 5 year survival rates, in the 33 cases who had received simultaneous bilateral neck dissection, were 81.5% (22/27) and 61.5%(16/26) respectively, while in the other 43 cases, was 69.2%(27/39) and 27.8%(10/36) respectively. CONCLUSION: It is necessary for those patients who have suffered from supraglottic carcinoma with bilateral neck metastasis to have simultaneous bilateral neck dissections. Meanwhile, the preservation of bilateral internal jugular veins is the best choice, at least one should be preserved.

Adult↗
PubMed2001

[Expression of rice dwarf virus outer coat protein gene(S8) in insect cells].

Outer coat protein gene(S8) of RDV was cloned into the transfer vector pVL 1393 to construct a recombinant vector pVL1393-S8. The recombinant vector pVL1393-S8 and the linear baculovirus RP23. LacZ were cotransfected into sf9 cells to produce the recombinant virus RP23-S8. RP23-S8 infected sf9 cells were collected and analysed by SDS-PAGE and Western-blot. The results showed that the S8 gene of RDV was expressed in sf9 cells and the expression level of sf9 cells was higher between 72-96 h after infected.

Animals↗
PubMed2001

[The effects of Ag+ on the absorption of trace metal ion during the somatic embryogenesis of Lycium barbarum. L].

We used callus of medical plant, Ningxia Lycium barbarum. L as material to induce somatic embryo genesis, and used multitracer technique and gamma-ray energy spectrum analytical method to study different concentration of AgNO3 on some absorption of trace metal ions during the somatic embryogenesis of Lycium barbarum. L. The results show that (1) when the concentration of AgNO3 is less then 50 mg/L, with the increase of AgNO3 concentration, the absorption rate of some metal ions is increasing, but when the concentration is excess of such concentration, the effects on the absorption rate are different. Ag+ has the effect of coordination, resistance or competition on the absorption of metal ions. (2) AgNO3 in suitable concentration will promote cell differentiation and somatic embryogenesis. When the concentration of AgNO3 is less then 50 mg/L, along with the increase of the concentration, the frequency of somatic embryogenesis is increased, and Ag+ has the promotion on the somatic embryogenesis of Lycium barbarum. L. While the concentration is up to 50 mg/L, it will mostly raise somatic embryogenesis in calli, the amount of callus is about 3 times as much as that of contrast group (in which AgNO3 is not added). When excess of this concentration point, Ag+ has toxic effect, and the somatic embryogenesis is inhibited remarkably.

Absorption↗
PubMed2001

[Study on the cytochrome P450 activity in alkane converting process of Candida tropicalis].

A method of reduced CO-difference spectrum was established to study the cytochrome P450 activity of the whole cell of Candida tropicalis during the alkane converting process. Using this method, the cytochrome P450 activities of the whole cells that were cultured in the different concentrations of alkane were studied. The results showed that the 5% alkane could induce the cytochrome P450 activity obviously but not inhibit the growth of cells, so it was determined preliminarily that the alkane concentration of the seed medium was 5%. The cytochrome P450 activities of dicarboxylic acid (DCA) fermentation processing were further studied. During the exponential phase of growth, the cytochrome P450 activity increased smoothly. However, during the phase of production of dicarboxylic acid, the cytochrome P450 activities increased rapidly after a sort decrease. The results still showed that the rate of production of dicarboxylic acid increased with the cytochrome P450 activity.

Alkanes↗
PubMed2001

[A pilot study on arsenic removal facilities for central supply of drinking water].

In order to promote the application and industrialization of an arsenic removal agent developed in this project, and to improve the technology, it is necessary to have a median sized facility to test the effects, capacity and regeneration of the agent. A facility with the capacity of 1 m3/h for arsenic removal was installed in a bottled water plant, which has been operated since April 1999. The results showed that the arsenic content of effluent water has met the requirement of the national standard for drinking water. The effect of arsenic removal was by 2 mg/g. The annual cost per person was only 1.46 Yuan RMB. The operation and maintenance of the facility was simple and no need to adjust the pH of influents and effluents. The chemical regenerative agent was safe, low cost and effective. Therefore, the facility can be used in rural areas and the water plants while the arsenic in source water was higher than 0.05 mg/L.

Arsenic↗
PubMed2001

Comparison of trans-scleral fixation of PMMA and foldable intraocular lens in children.

PURPOSE: To observe the difference of the effects of PMMA and foldable intraocular lenses (IOLs) trans-sclerally fixed in pediatric eyes. METHODS: Thirty-two children (43 eyes) who had undergone trans-scleral fixation of IOL were retrospected, of whom 5 children were implanted PMMA IOL in both eyes, 6 children were implanted PMMA IOL in one eye and foldable IOL in the other eye, 12 children were implanted foldable IOL in one eye and 9 children were implanted PMMA IOL in one eye. Mean age was 5.3 years (range 2.5-12 years). Twelve children had traumatic cataract and the others congenital cataract before lens extraction. RESULTS: Foldable group (18 eyes): Mean follow-up was 12.1 months. Visual acuity (VA): compared with the best corrected VA before IOL fixation, postoperative best corrected VA improved in 16 eyes, remained unchanged in 2 eyes. In 14 eyes, one or two stitches were needed to seal the incision. COMPLICATIONS: Severe anterior chamber reaction was seen in 3 eyes. Intraocular bleeding was found in 3 eyes. IOL decentration was detected in 1 eye. Iris capture of IOL was seen in one eye. PMMA group (25 eyes): Mean follow-up was 20.3 months. Visual acuity (VA): compared with the best corrected VA before IOL fixation, postoperative best corrected VA improved in 19 eyes, remained unchanged in 5 eyes and got worse in one eye. In 24 eyes, one to three stitches were needed to seal the incision. COMPLICATIONS: Severe anterior chamber reaction was seen in 5 eyes. Intraocular bleeding was found in 4 eyes. IOL decentration was seen in one eye. Iris capture of IOL was seen in 3 eyes. Intraocular pressure elevated in one eye. CONCLUSION: Our study shows that trans-scleral fixation of IOL is a safe procedure in pediatric eyes. Foldable IOL showed similar effect compared with PMMA IOL in pediatric trans-scleral fixation.

Anterior Chamber↗