Search PubMed⌕ Search

Biomedical subjects

S Levi

Publications and source records attributed to S Levi.

At least 199 records · Page 11Linked to original sources
PubMedDate not supplied

Structure, function, and evolution of ferritins.

The ferritins of animals and plants and the bacterioferritins (BFRs) have a common iron-storage function in spite of differences in cytological location and biosynthetic regulation. The plant ferritins and BFRs are more similar to the H chains of mammals than to mammalian L chains, with respect to primary structure and conservation of ferroxidase center residues. Hence they probably arose from a common H-type ancestor. The recent discovery in E. coli of a second type of iron-storage protein (FTN) resembling ferritin H chains raises the question of what the relative roles of these two proteins are in this organism. Mammalian L ferritins lack ferroxidase centers and form a distinct group. Comparison of the three-dimensional structures of mammalian and invertebrate ferritins, as well as computer modeling of plant ferritins and of BFR, indicate a well conserved molecular framework. The characterisation of numerous ferritin homopolymer variants has allowed the identification of some of the residues involved in iron uptake and an investigation of some of the functional differences between mammalian H and L chains.

Amino Acid Sequence↗
PubMedDate not supplied

Functional roles of the ferritin receptors of human liver, hepatoma, lymphoid and erythroid cells.

Ferritin receptors are present on the membranes of many normal and malignant cells. The binding specificity of these receptors for H and L subunits was examined using recombinant human ferritin homopolymers. At least two different types of ferritin receptors were found, one derived from normal rat, pig, and human liver which shows similar binding of H- and L-ferritin. The second receptor type, specific for the H-chain ferritin, has been identified on membranes of hepatic and other transformed cells, and of normal lymphoblasts and erythroid precursors. These two receptor types may have different metabolic functions: the hepatic receptor acting as a scavenger for circulating ferritin and possibly for iron exchange between hepatocytes and macrophages; the H-ferritin receptor having a regulatory role which is not directly related to iron metabolism. The expression of the H-ferritin receptor is closely related to the activation and proliferation state of the cells. Addition of H-ferritin to the culture medium of cells expressing the H-ferritin receptor resulted in inhibition of cell proliferation and of colony formation.

Animals↗
PubMedDate not supplied

[Fetal biometry: review and application of sex determination].

Diagnostic ultrasound was introduced in the practice of obstetrics through its ability to measure accurately the biparietal (DBP) diameter of the foetus. The development of bidimensional and grey tone images expanded the possibility of fetal biometry. One of its most important uses is the estimation of gestational age. A knowledge of foetal age is necessary to interpret the results of most of the investigations currently performed during pregnancy. Different fetal dimensions increase with age at different rates according to the period of gestation. During the embryonic period, the crown-rump length gives a very good estimation of age within 4-6 days for twice the standard deviation. Later on, because of the curvature of the fetus, the age is estimated with the DBP. The accuracy diminishes with the slowing down of fetal growth, from 12 weeks onwards, when twice the standard deviation of the age estimate may be as long as 2 weeks. When DBP is no longer reliable in determining age, femur length is measured being more reliable for that purpose at least till 24 weeks. A very early assessment of foetal age reduces the risk of errors in estimating the duration of pregnancy and is important to detect early growth retardation (IUGR); this is usually symmetric, i.e. affecting all fetal parts (versus asymmetrical or late IUGR where trunk growth is first involved). Fetal weight can be estimated by fetal parts measurements. A formula was derived as long as twenty years ago but not all authorities agree on any measuring technique as evidenced by the many different formulas proposed--a sign that no very satisfactory one yet exists.(ABSTRACT TRUNCATED AT 250 WORDS)

Anthropometry↗
PubMedDate not supplied

[Normal and pathological ultrasonography of the fetal brain].

Advanced ultrasonic technology enabled the bidimensional imaging of fetal brain throughout pregnancy. The number of visible structures increases with gestational age. In order to certify the described items they were compared to similar anatomical sections available from brain specimens or an atlas of anatomy. Real-time as well as automatic compound scanning machines were used to obtain echographic images of fetal brain anatomy in utero. Many different scans were collected along three orthogonal directions: every 2 mm, with the automated compound scan and as close from each other as possible with the linear array real-time scanner. The material included 59 normal fetuses between the 15th and 40th week of gestation. Eight typical sections are made but the only structures identified are those being visible on every section obtained at the same level. Brain structures such as grey and white matter, nuclei, vessels and ventricles have different sonic properties. They are subsequently distinguishable on the ultrasonic image if the equipment is capable of detecting and showing such differences. Grey matter is more echogenic than white, choroid plexuses are very reflective as are vessel walls which also pulsate. Four horizontal sections are described in detail (Figs. 2-7). The coronal planes are demonstrated at three different levels (Figs. 8-12) and the sagittal plane is represented by Fig. 13. Thirty five structures were identified (Table 2). In the second section some brain and skull anomalies are briefly described. The principal signs of the abnormalities and possible encountered variations are listed such as cranial defects in anencephaly, acrania, encephalocele, iniencephaly; the anomaly of size and shape of head and ventricle, as in hydrocephaly and microcephaly, cystic-like- and solid-tumor lesions and Dandy Walker syndrome and hydranencephaly. Attention is drawn to possible artifacts and pitfalls in the differential diagnosis between similar looking pictures. Examples are given such as noise in the near field, occurring more often in sectorscan machines, inadequate orientation of scan plane producing asymetrical display of the hemisphere and the Sylvian fissure described as if it was the lateral ventricle wall. Anomalies can also be confused such as exencephaly, anencephaly, iniencephaly, encephalocele. An excellent knowledge of normal brain anatomy and pathology, together with excellent equipment and a careful scrutiny of the echograms are required qualities for fetal brain examination.

Anencephaly↗
PubMedDate not supplied

New aspects of fetal dynamics with a special emphasis on eye movements.

During the last few years, special attention has turned to the recording of human fetal dynamic phenomena. These are movements of the whole body as well as breathing, cardiac and ocular movements. All of them can be observed and recorded simultaneously through real-time echography and Doppler systems. The analysis of the recording may be considered separately as well as simultaneously and are one of the grounds for fetal physiology studies. What is known about fetal dynamics and physiopathology is summarized. Fetal biorhythms were discovered and described in that manner. Progressive integration in behavioural patterns was observed suggesting that movements are under a general and central control early in fetal life. Particular attention is given to personal work on fetal eye movement. Based on eye movements patterns, the fetal behaviour patterns might be compared to the three sleep stages (paradoxal--quiet--transitional) as described in the full-term and premature newborns. Some contradictions have been noticed in the conclusions drawn from the analysis of the fetal activities considered separately. They might only be apparent in the light of these integrated patterns.

Eye Movements↗
PubMedDate not supplied

Vindaloo and you.

Explore the source record for details and available documents.

3-O-Methylglucose↗
PubMedDate not supplied

Patients' attitudes towards the use of gloves by healthcare staff.

There is a concern among healthcare providers regarding the use of gloves in non-mandated clinical situations and its impact on patient sensitivities. A convenience sample of 76 Israeli hospital inpatients were surveyed to measure patients' attitudes towards the use of gloves by healthcare providers. The Patient Sensitivity Questionnaire (PSQ) was developed and used to collect the data. Overall, patients were found to have a positive attitude towards the use of gloves in most patient care situations. Significant differences in patient attitudes towards glove use were found based on country of origin and number of hospital admissions in the previous year. The study concluded that hospitalized patients, in general, support healthcare providers' use of gloves. In addition, subjects agreed with the (American) Occupational Safety and Health Administration regulations regarding glove use.

Attitude to Health↗