[Psychology and sexology in the general hospital].
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Adult↗
Biomedical subjects
S Lefort
Publications and source records attributed to S Lefort.
Hydrophobic adsorbents for the isolation and purification of biosynthetic human growth hormone from crude fermentation mixtures.
Hydrophobic interaction chromatography was used as the initial step for the concentration and partial purification of biosynthetic human growth hormone (hGH). This molecule was extracted from the culture medium of a transformed monkey kidney cell line by batch adsorption either to Octyl-Sepharose or Phenyl-Sepharose. The adsorbate was decanted and packed into a column for elution. This initial work-up procedure rendered a concentrated hGH solution that was further purified by ion-exchange and gel filtration chromatography. The hGH obtained was homogeneous, based on polyacrylamide gel electrophoresis, N-terminal sequence and fingerprint analysis.
Adsorption↗
[Program for nurse practitioners].
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Curriculum↗
The biological activities of gro beta and IL-8 on human neutrophils are overlapping but not identical.
Gro beta and IL-8 are two members of the small induced secreted (SIS) cytokine family (C-X-C subgroup) with proinflammatory activities on neutrophils. In order to assess whether or not the interaction with their receptors results in similar biological actions, we compared the two cytokines in five different bioassays. Gro beta showed similar biological activities as IL-8 in tests of chemotaxis, induction of the respiratory burst, and induction of interleukin 6 (IL-6) production. However, for two other biological activities: augmentation of the expression of CD11b on the cell surface and rapid elevation of the intracellular calcium concentration, maximal effects required 100 times more gro beta than IL-8. Taken together, these results suggest that the stimulation of the IL-8 or gro beta receptor evokes three similar responses, but that only the activation of the IL-8 receptor and not that of gro beta results in elevated CD11b expression and calcium mobilization in human neutrophils.
Antimicrobial Cationic Peptides↗