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Biomedical subjects

S Le Moli

Publications and source records attributed to S Le Moli.

At least 37 records · Page 2Linked to original sources
PubMed1985

Isotypic analysis of antibody response to a food antigen in inflammatory bowel disease.

We studied the class-specific antibody response to the cow's milk antigen beta-lactoglobulin (beta-LG) in sera from patients with ulcerative colitis and Crohn's disease. IgG and IgM to beta-LG were significantly higher in patients when compared to healthy non-atopic controls, whereas IgA values were similar, and specific IgE absent in all groups. No correlation between IgG- or IgM-containing immune complexes was found with the corresponding isotype of antibody to beta-LG; however, IgM complexes correlated with serum total IgM in ulcerative colitis. In these patients, IgG antibodies were higher in active cases, whereas IgM increased in patients without signs of disease activity. Antibody titers did not correlate with disease duration or administration of antiinflammatory drugs. This pattern of anti-beta-LG reactivity suggests that the presence of intestinal lesions may be revealed by the selective increase of some antibody isotypes to orally administered antigens. Enhanced mucosal permeability may be studied by this type of serological analysis.

Adrenal Cortex Hormones↗
PubMed1984

Chronic granulomatous disease in two sisters.

Two sisters with chronic granulomatous disease (CGD) have been studied. The diagnosis was suggested by the histopathological findings from the spleen and lymph nodes of the proband and confirmed by the low values obtained in the following tests performed on polymorphonuclear leukocytes (PMN): chemiluminescence, nitroblue tetrazolium (NBT) reduction, killing of Staphylococcus aureus, and O2- production. NADPH oxidase activity was not detected in the homogenates of the patients' PMN but cytochrome b was normally present. In addition, PMN depolarization induced by phorbol-myristate acetate was absent, thus suggesting a defect of the activation mechanism of the respiratory enzyme. The normal depolarization induced by ouabain indicated that the membrane polarity regulated by the Na/K pump in the patients' cells was not affected. The low, but not completely absent, respiratory activity of the patients' PMN could suggest an X-linked mode of inheritance with incomplete Lyonization. From a clinical point of view, one sister had mild symptoms whereas the other was almost symptomless, thus confirming once more the heterogeneity of CGD syndrome.

Adolescent↗
PubMed1983

In vitro and in vivo effect of sisomicin and gentamycin on polymorphonuclear chemotaxis and phagocytosis.

Ten patients with acute bacterial infections were studied to demonstrate the influence of two aminoglycosides, sisomicin and gentamycin on polymorphonuclear (PMN) chemotactic and phagocytic functions. Five of these subjects were treated with gentamycin and five with sisomicin both at a dosage of 2.5 mg kg-1 day-1. The assays were performed before and after 5 days of therapy. Before beginning treatment, the in vitro action of the aminoglycoside antibiotics (20 micrograms ml-1) was also tested. Granulocytes from 8 healthy people were used as controls. After therapy, no significant difference in basic values was found in either the gentamycin treated or in the sisomicin treated group. Similar results were obtained in incubation tests on both patients and healthy subjects. In conclusion, we conclude that the use of these mentioned aminoglycoside antibiotics, at the therapeutical doses employed, does not interfere with the PMN functions.

Adult↗
PubMed1983

In vitro effect of ketoconazole on human neutrophil chemotaxis and Candida albicans killing.

Ketoconazole, an antifungal agent, was investigated to discover its possible enhancing role on polymorphonuclear chemotactic and Candida killing functions in vitro. Polymorphonuclear leukocytes from 10 healthy subjects and two different strains of Candida albicans were used. At concentrations of 1 microgram/ml Ketoconazole showed no distinct synergistic effect on either of the above-mentioned polymorphonuclear cellular functions, but, on the other hand it operated directly on Candida cells. In addition, this drug did not demonstrate any chemotactic activity on granulocytes. These data could suggest that host cellular defence and Ketoconazole exert parallel rather than synergistic activity.

Candida albicans↗
PubMed1982

Serum and salivary IgA levels in normal subjects: comparison between tonsillectomized and non-tonsillectomized subjects.

Serum IgA and secretory IgA (SIgA) were determined in 1,000 apparently healthy subjects; 274 were tonsillectomized. Four groups were identified in this study: (1) 0.3% of subjects had no serum IgA and SIgA; (2) 1.6% of subjects showed partial serum and SIgA deficiency; (3) 27.4% of individuals were tonsillectomized and had partial serum IgA deficiency but normal SIgA, and (4) 71.4% were normal, both with respect to serum IgA and SIgA. There results stress the relatively high incidence of IgA deficiency in the normal population and confirm the role of the tonsils in maintaining serum IgA levels.

Adolescent↗
PubMed1981

In vitro studies on cellular and humoral chemotaxis in Crohn's disease using the under agarose gel technique.

The locomotor function of polymorphonuclear cells (cellular chemotaxis) and serum chemotactic activity (humoral chemotaxis) were studied in 51 patients with Crohn's disease using a method of migration under agarose gel. To study cellular chemotaxis patient's polymorphonuclear cells were challenged against normal Zymosan activated serum and humoral chemotaxis was evaluated testing the patient's Zymosan activated serum against normal polymorphonuclear cells. Cellular chemotaxis in the Crohn's disease group was normal (although 30% of the 51 patients had migration values out of the normal range), while humoral chemotaxis was significantly lower in Crohn's disease patients than in the control group. However, the value of humoral chemotaxis in the group of Crohn's disease patients treated with steroids was lower than that of patients not treated, thus accounting for the low mean value observed inthe Crohn's disease-group as a whole. The present results suggest that a defective chemotactic response may occur in some Crohn's disease patients, particularly during steroid treatment. These findings might be related either to a defective generation of complement derived chemotactic factors or to the presence of circulating inhibitors.

Adrenal Cortex Hormones↗
PubMed1979

[Circulating immunocomplexes in patients with various morbid conditions].

The polyethylenglycol (PEG) precipitation technique has been employed for the measurement of immune complexes in the circulation of 100 normal subjects and in 14 patients suffering from a variety of diseases (systemic lupus erythematodes, nephrosic syndrome, cryoglobulinaemia, Buckley's syndrome). Values higher than 0.80 UA on the absorption scale were considered pathological, namely 2 standard deviations above the mean (0.32 UA) in the subjects examined; in the patients, values between a minimum of 0.98 UA and a maximum of 2.38 UA were observed. Longitudinal study of these cases also pointed to the disappearance of immune complexes during therapy. The results suggest that the PEG precipitation technique can play an important part as a screening test in situations in which circulating IC pathology is suspected; it is also a sensitive means of monitoring treatment.

Adolescent↗
PubMed1979

Study of chemotaxis by a modified method of migration under agarose gel.

In this paper the standardization of a method for studying chemotaxis of polymorphonuclear cells under agarose gel in vitro on 20 adult normal subjects is reported. An original method of reading is emphasized in which the maximum migration distance and the number of migrated cells are held in consideration.

Blood↗