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S L Boyer

Publications and source records attributed to S L Boyer.

4 recordsLinked to original sources
PubMed2001

Is the 16S-23S rRNA internal transcribed spacer region a good tool for use in molecular systematics and population genetics? A case study in cyanobacteria.

We amplified, TA-cloned, and sequenced the 16S-23S internal transcribed spacer (ITS) regions from single isolates of several cyanobacterial species, Calothrix parietina, Scytonema hyalinum, Coelodesmium wrangelii, Tolypothrix distorta, and a putative new genus (isolates SRS6 and SRS70), to investigate the potential of this DNA sequence for phylogenetic and population genetic studies. All isolates carried ITS regions containing the sequences coding for two tRNA molecules (tRNA and tRNA). We retrieved additional sequences without tRNA features from both C. parietina and S. hyalinum. Furthermore, in S. hyalinum, we found two of these non-tRNA-encoding regions to be identical in length but different in sequence. This is the first report of ITS regions from a single cyanobacterial isolate not only different in configuration, but also, within one configuration, different in sequence. The potential of the ITS region as a tool for studying molecular systematics and population genetics is significant, but the presence of multiple nonidentical rRNA operons poses problems. Multiple nonidentical rRNA operons may impact both studies that depend on comparisons of phylogenetically homologous sequences and those that employ restriction enzyme digests of PCR products. We review current knowledge of the numbers and kinds of 16S-23S ITS regions present across bacterial groups and plastids, and we discuss broad patterns congruent with higher-level systematics of prokaryotes.

Base Sequence↗
PubMed1984

The spouse's adjustment to chronic pain: cognitive and emotional factors.

Recent research has demonstrated that the pain behaviors displayed by patients who have chronic pain complaints can be rewarded and maintained by the solicitous and attentive responses of spouses. The present study examines cognitive and emotional factors which may underly such solicitous responding by the spouses. In this study spouses completed a questionnaire (The Spouses' Perception of Disease--SPOD) which was designed to determine their perceptions of the patients' chronic pain syndromes. Additionally spouses completed a measure of emotional and psychological functioning (SCL-90) and a measure of marital satisfaction (Locke-Wallace Scale). The results of this study show that spouse's cognitive interpretation of the patient's chronic pain syndrome is closely associated with the spouse's emotional adjustment and marital satisfaction. Optimism, perception that the patient has a positive attitude along with few psychological problems and the perception that the patient is severely disabled all are associated with more positive emotional status in spouses. The results are discussed in terms of their implications for the rehabilitation of chronic pain patients.

Adaptation, Psychological↗
PubMedDate not supplied

The HL7 Clinical Document Architecture.

Many people know of Health Level 7 (HL7) as an organization that creates health care messaging standards. Health Level 7 is also developing standards for the representation of clinical documents (such as discharge summaries and progress notes). These document standards make up the HL7 Clinical Document Architecture (CDA). The HL7 CDA Framework, release 1.0, became an ANSI-approved HL7 standard in November 2000. This article presents the approach and objectives of the CDA, along with a technical overview of the standard. The CDA is a document markup standard that specifies the structure and semantics of clinical documents. A CDA document is a defined and complete information object that can include text, images, sounds, and other multimedia content. The document can be sent inside an HL7 message and can exist independently, outside a transferring message. The first release of the standard has attempted to fill an important gap by addressing common and largely narrative clinical notes. It deliberately leaves out certain advanced and complex semantics, both to foster broad implementation and to give time for these complex semantics to be fleshed out within HL7. Being a part of the emerging HL7 version 3 family of standards, the CDA derives its semantic content from the shared HL7 Reference Information Model and is implemented in Extensible Markup Language. The HL7 mission is to develop standards that enable semantic interoperability across all platforms. The HL7 version 3 family of standards, including the CDA, are moving us closer to the realization of this vision.

Computer Communication Networks↗