Cystic fibrosis in an Oriental child.
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Asian People↗
Biomedical subjects
S Kwok
Publications and source records attributed to S Kwok.
Suppression of lymphocyte mitogenesis by tamoxifen.
Tamoxifen (at 0.1-1 microM concentrations) inhibited significantly the response of rat spleen cells to phytohemagglutinin, concanavalin A, pokeweed mitogen and lipid A in a dose-dependent manner. To achieve this inhibition, it was sufficient to expose the lymphocytes for 4 h to tamoxifen prior to mitogenic stimulation. Estradiol did not have a consistent effect on lymphocyte mitogenesis under the same conditions and did not modify the suppressive effect of tamoxifen, even when the lymphocytes were treated first with estradiol followed by tamoxifen. It is suggested that the inhibitory effect of tamoxifen on these in vitro lymphocyte reactions is not mediated by the estrogen receptor.
Animals↗
The influence of estradiol and tamoxifen on the mixed lymphocyte reaction in rats.
The effect of estradiol and of the anti-estrogenic agent, tamoxifen, on mixed lymphocyte reactions was examined in the rat. Tamoxifen exerted a consistent inhibiting effect at concentrations ranging from 0.4-400 ng/ml. Estradiol was ineffective at physiological concentrations (0.4 ng/ml), whereas at pharmacological levels (400 ng/ml) significant inhibition was observed in some experiments but not in others. The inhibitory effect of both agents was more consistent in fetal calf serum containing medium when compared to that with autologous serum. Phenol Red had no effect on the outcome of these experiments.
Animals↗
Low incidence of HTLV infections in random blood donors with indeterminate western blot patterns.
Peripheral blood mononuclear cells (PBMCs) were recovered from platelet units of 61 blood donors who were HTLV-I positive and 3 blood donors who were HTLV-I negative on enzyme-linked immunosorbent assay (ELISA). Western blot analyses were performed on the sera and DNA was prepared from the PBMCs and analyzed by the polymerase chain reaction (PCR). Of the 61 repeatably reactive samples, 2 were positive, 26 were negative, and 33 were interpreted as indeterminate on Western blot. HTLV-II sequences were detected by PCR in one of the Western blot-positive samples, as well as in one Western blot-indeterminate sample that showed reactivity to p24 only. HTLV-I sequences were detected in the second Western blot-positive sample. HTLV sequences were not detected in the remaining samples, which suggested that the majority of individuals with indeterminate results on Western blots that used one set of commercially available reagents are not infected with HTLV. It is demonstrated in this study that PCR can be used not only to resolve the infection status of individuals with indeterminate Western blots but also to distinguish between HTLV-I and HTLV-II.
Blood Donors↗