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Biomedical subjects

S Kurihara

Publications and source records attributed to S Kurihara.

At least 235 records · Page 13Linked to original sources
PubMed1978

Complement-fixing pemphigus antibodies.

The serum of a patient with a typical case of pemphigus vulgaria contained complement-fixing intercellular autoantibodies (pemphigus antibodies). Complement-fixing pemphigus antibodies were demonstrated only during the untreated active stage of the disease and titrated lower than corresponding IgG autoantibodies, which paralleled well with the disease activity. Pemphigus lesional skin, which contained in-vivo-bound IgG, showed the capability of further binding C3 in vitro from normal human serum. It was suggested from these findings that the complement system may play an active role in pemphigus acantholysis through complement-fixing pemphigus antibodies.

Acantholysis↗
PubMed1977

Capability of complement fixation of pemphigus antibodies in vitro.

The capability of complement fixation of pemphigus antibodies was tested using combined in vitro complement immunofluorescent (IF) staining methods. Three sera out of 25 serum samples from 22 pemphigus patients revealed positive reactions, while all other sera gave negative results. Specificity control tests confirmed the positive reactions to be specific for complement staining. Complement fixing pemphigus antibodies were titrated lower than corresponding IgG antibodies and were demonstrable only in the extensive stage of the disease. Thus, the present work supplied evidence that pemphigus antibodies fix complement in vitro. However, the discrepancy still remains between the in vivo deposition of complement in most cases of pemphigus and in vitro capability of complement fixation in only few cases. More investigations should be needed to explain the exact role of complement in pemphigus acantholysis.

Antibodies↗
PubMed1977

An electron microscopic observation on cells found in bone resorption area incident to experimental tooth movement.

For the purpose of investigating many of the cells in the bone resorption area incident to experimental tooth movement and characterizing the role of these cells for the periodontal tissue resorption at the ultrastructural level, orthodontic force was applied to 24 male rats. Orthodontic elastics were inserted into the interproximal space of the upper first and second molars. The mesial side of the interradicular septum of the second molar was observed. The electron micrsocopic findings were as follows: 1. Cell cohorts were found in the undermining bone resorption area. They are mainly composed of fibroblasts, endothelial cells, undifferentiated cells, macrophages and several kinds of osteoclasts. 2. Four different kinds of osteoclasts could be recognized from the morphological features: a) small osteoclasts situated apart from the bone surface, b) large osteoclasts rich in rough ER, c) classic large osteoclasts and d) degenerating osteoclasts. 3. Two different types of undifferentiated cells were observed in these area. One was a spindle-shaped bright cell rich in rough ER and the other was a round-shaped dark cell with numerous mitochondria and free ribosomes. 4. Macrophages showing phagocytosis were also found in the bone resorption area.

Animals↗
PubMed1977

Establishment of a human leiomyosarcoma cell line.

A cell line designated SKN was established from the human uterine leiomyosarcoma of a 52-year-old female. The cell line has grown well and the serial passages were successively carried out 82 times within 12 months. The monolayer cultured cells revealed anaplastic and pleomorphic features, and they multipled rapidly without contact inhibition. Electron microscope studies revealed myoibrils but no virus-like particles, while chromosomal studies showed that all cultured cells were hyperploid, the modal number was 112, and the marker chromosome was present. The cells were transplanted into an immune-depressed hamster cheek pouch and produced a histological leiomyosarcoma resembling the original tumor.

Animals↗
PubMed1976

Serum antibodies to herpesvirus early antigens in patients with cervical carcinoma determined by anticomplement immunofluorescence technique.

Sera from patients with carcinoma of the uterine cervix were used with the anticomplement immunofluorescence (ACIF) technique to develop a simple procedure for detection of antibodies to herpes simplex virus type 2 (HSV-2) early antigens. Test cells used in the ACIF assay were HEp-2 cells infected with HSV-2 sequentially treated with inhibitors of protein and of DNA SYNTHESIS. The cells were first treated for 7 h with cycloheximide (100 mug/ml) and then for 3 h with hydroxyurea (150 mug/ml). In this assay serum titers giving more than 21.5% ACIF-positive cells correlated to invasive carcinoma of the cervix. Using these criteria 18 out of 43 patients with invasive carcinoma, none of 8 patients with carcinoma in situ, and only 1 of 43 controls, were diagnosed as positive. Thus an elevated titer of antibodies against HSV-2 antigens correlated to cervical carcinoma.

Antibodies, Viral↗
PubMed1976

Relationship between effects of procaine and Ca on spontaneous electrical and mechanical activities of the smooth muscle cells of the guinea pig urinary bladder.

The relationships between the effects of procaine and those of Ca on the spontaneous electrical and mechanical activities of smooth muscle cells of the guinea pig urinary bladder were studied using both microelectrode and sucrose gap methods. Procaine(1, 7, and 15mM) depolarized the membrane and increased spontaneous spike freqency. The maximum rates of rise and fall and the after-hyperpolarization of the spike decreased. Enhanced spontaneous rhythmic contractions accompanied these membrane activities (1 mM). The tone of the preparation (resting rension) increased transiently and decreased gradually (7-15mM). These procaine effects were suppressed by the application of excess Ca (7.5 mM). The increased electrotonic potential induced by 7mM procaine was reduced by the addition of 7.5 mM Ca. Reduction of Ca concentration (0.8 and 0 mM) IN Krebs solution containing procaine further depolarized the membrane and decreased the spike amplitude. In Ca-deficient solution, the enhanced rhythmic contractions induced by procaine were slightly reduced. Compound D600 (1-3mg/1), manganese ion (Mn) (1-3 mM), and cobalt ion (CO) (1-3 MM) inhibited the effects of procaine on spike discharges and mechanical responses. From these results it is concluded that procaine action is related to that of Ca which controls the membrane permeability, since procaine depolarizes the membrane by reducing K conductance. Furthermore, action potentials observed in the Krebs solution containing procaine are considered to be Ca spikes, which seem to play an important role in excitation-contraction coupling in the guinea pig urinary bladder smooth muscle.

Action Potentials↗
PubMed1976

Inhibitory effects of procaine on the electrical and mechanical activities of the smooth muscle cells of the guinea pig urinary bladder.

Modification of the effects of K, Ca, acetylcholine and rapid cooling by procaine were examined in the smooth muscle of the guinea pig urinary bladder. Contraction enhancement by procaine was observed in 5.9 mM K - and 23.6 mM K-Krebs solution but was not confirmed in 59 mM K solution. Procaine enhanced contraction in relation to membrane activities. Relationships between membrane potential and K concentration showed that procaine reduced K conductance. This effect decreased as K concentration increased. K-induced contracture was inhibited by procaine at 37 degrees and 10 degrees C. Procaine inhibited Ca- and acetylcholine-induced contractions in isotonic K2SO4-Locke solution. Rapid cooling contracture, initiated by lowering the temperature of the solution from 36 +/- 1 degrees C to 12 +/- 2 degrees C within 30 sec, was inhibited by procaine. From these results, it is concluded that procaine has an inhibitory effect on Ca permeation through the cell membrane and on Ca release from sequestered sites, and it is confirmed that procaine enhancement of spontaneous contractions is related to the spike activity resulting from depolarization which is caused by reduction of K conductance (KURIHARA, 1975). The mechanism of the procaine effect is discussed in relation to excitation-contraction coupling in the guinea pig urinary bladder.

Acetylcholine↗
PubMed1976

Effect of dantrolene sodium on excitation-contraction coupling of frog toe muscle.

Dantrolene sodium (1.0 to 10.0 mug/ml) inhibited twitch tension, tetanus tension, and contracture induced by potassium and low concentration of caffeine in the frog toe muscles. The drug also inhibited rapid cooling contracture (RCC), i.e., contracture caused by rapidly cooling the muscle from room temperature to 0 degrees C after immersing it in Ringer's solution containing a subthreshold concentration of caffein. The drug also suppressed RCC in depolarized muscle fibres which were treated with high concentration of potassium. Comparing the effect of the drug on twitches augmented with nitrate and with caffeine, the former was more markedly inhibited than the latter. On the basis of these results, the possibility that the drug acts mainly by inhibiting the movement of "trigger calcium" which in turn releases calcium from the sarcoplasmic reticulum is discussed.

Animals↗