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Biomedical subjects

S Krilis

Publications and source records attributed to S Krilis.

26 records · Page 2Linked to original sources
PubMed1981

Investigation of the possible involvement of IgE anti-salicyloyl antibodies in patients with urticaria.

19 aspirin-sensitive and 14 non-aspirin-sensitive patients with chronic urticaria were tested for the presence of specific anti-salicyloyl-IgE antibodies by prick testing, RAST and in vitro histamine release using an aspirin-polylysine conjugate. Polylysine succinate served as a control. Negative results were obtained in all cases with the exception of 1 patient who gave a positive prick test response. However, this patient reacted to the control conjugate as well as aspirin polylysine. Skin prick test responses to the battery of environmental allergens revealed that the majority (90%) of the aspirin-sensitive patients were non-atopic. The findings suggest that the clinical symptoms and signs in patients with chronic urticaria associated with aspirin sensitivity are not mediated by specific IgE antibodies.

Aspirin↗
PubMed1980

Hypersensitivity to inhaled flour allergens. Comparison between cereals.

Radioallergosorbent testing (RAST) of sera from subjects sensitized to wheat and rye flour indicated that there is significant reaction with seed extracts of 12 cereals (wheat, durum wheat, triticale, cereal rye, barley, rye grass, oats, canary grass, rice, maize, sorghum and Johnson grass). Results were evaluated in terms of taxonomic relationships and of the electrophoretically determined protein composition of the cereal extracts. RAST uptakes were uniformly low in sera from four rhinitic bakers, yet were significantly above the levels for non-allergic and cord sera. Much higher RAST uptakes were obtained with sera from four asthmatic bakers when tested with wheat and its close relatives, but there was still reasonably high reactivity with more distantly related cereals. RAST inhibition experiments indicated in a more direct way the extent of cross-reactivity between grain extracts of wheat, rye, barley and oats. One baker had a history of more severe attacks of breathlessness following inhalation of rye flour compared with wheat flour. This was confirmed by bronchial challenge testing, but the comparison was not obviously consistent with the results of prick testing or estimation of histamine released from his leucocytes. The results as a whole suggested that the bran layers of cereal grains are at least as allergenic as flour.

Allergens↗
PubMed1980

An enzymatic-isotopic microassay for measuring allergic release of histamine from blood and mast cells in vitro.

An enzymatic-isotopic microassay has been developed which has the sensitivity and specificity to measure picogram amounts of histamine in 20 microliter samples of plasma. This analytical technique has been used to study the allergic release of histamine in vitro in whole human blood. Prior separation of leucocytes is not necessary and multiple assays can be performed on minimal blood samples making the procedure attractive as a diagnostic procedure in allergy. The enzymatic-isotopic microassay is versatile in that it has also been applied to the study of all allergic release of histamine from rat peritoneal mast cells.

Allergens↗
PubMedDate not supplied

15-hydroxyeicosatetraenoic acid synthesis from exogenous arachidonate by bronchial mucosa.

Arachidonate metabolism in bronchial mucosa was investigated. Bronchial biopsies from four asthmatics and three non-asthmatic controls were challenged with 300, 465, 650 and 960 mOsm/kg saline in the presence of [3H]arachidonate. Supernatants were analysed by reverse-phase high-performance liquid chromatography. The major arachidonate metabolite was identified as 15-monohydroxyeicosatetraenoic acid (15-HETE), with significantly higher production in biopsies from asthmatics than controls. Statistical analysis of the full model explained 81% of the variation (F = 14.8; df = 6, 21; P less than 0.001). Hyperosmolarity and the presence of sarcoid had no significant effect on 15-HETE synthesis, reducing R2 by only 6%.

Adult↗