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Biomedical subjects

S Konno

Publications and source records attributed to S Konno.

At least 217 records · Page 12Linked to original sources
PubMed1981

Impalement wounds of the head and chest by reinforced steel bars with recovery: an unusual case report.

A 31-year-old male who sustained completely penetrating impalement wounds of the head and chest by reinforced steel bars in a fall at a ferroconcrete building construction with a miraculous survival is reported. The bars were successfully removed; a surgical mallet was required to loosen the bar impacted in the patient's head. After 12 days he was discharged, and he has returned to construction work and is well 3 years postinjury.

Accidents, Occupational↗
PubMed1981

Morphology of sulfur granules produced by Pseudomonas aeruginosa in cows.

A sulfur granule produced by Pseudomonas aeruginosa in three cows was studied light- and electron-microscopically. It consisted of clumps of basophilic bacteria and eosinophilic clubs. The clubs radiated from the periphery outward. The bacteria were stained dark red by both PAS and MacCallum-Goodpasture staining. Electron-microscopically, the sulfur granule consisted of electron-dense amorphous material and bacteria. The intact bacterium was about 0.45 micrometers in diameter and had an electron-dense cell wall 15 nm in width. Beneath the cell wall, moderately electron-dense fine granular material was present. The center of the bacterium was electron lucent. The club was electron-dense amorphous material. Degenerative bacteria and pilus-like structures were often seen in it. In one case, clusters of bacteriophages were present in and near the degenerative bacteria. Their head was hexagonal and 40 nm in diameter.

Animals↗
PubMed1981

Electron microscopy of cells cultured in serum-free medium after inoculation of swine vesicular disease virus.

Morphological alterations of IB-RS-2 cells cultured in serum-free maintenance medium after inoculation of swine vesicular disease virus (SVDV) were studied electron-microscopically. Cells harvested 0 to 3 hours after inoculation showed no alterations. Cellular alterations were observed from 4 to 7 hours after inoculation. Many vacuoles appeared just beneath the cytoplasmic membrane and were separated by thin cytoplasm. Narrow pathways were sometimes seen in the degenerative cells. They occasionally ran from the vacuole just beneath the cytoplasmic membrane to the nuclear cistern. Ribosome-like granules were seen along the narrow pathway or diffusely in the cytoplasm, or accumulated to form an islet. Membrane-bound bodies were frequently noticed in the central region of the cell. Cytoplasmic blebs were sometimes seen projecting from the cell surface. Vacuoles, narrow pathways and cytoplasmic blebs were absent in cells cultured in the medium with serum. So serum might exert some effects on the cytoplasmic membrane or cytoskeletal framework. Crystalline arrays of SVDV were found in the cytoplasm of degenerative cells harvested from 4 to 7 hours after inoculation. They were near the ribosome-like granules and membrane-bound bodies, but had no relationship with vacuoles just beneath the cytoplasmic membrane and narrow pathway. Their size and morphology were the same as those seen in the cells cultured in the medium with serum.

Animals↗
PubMed1980

Crystal formation of swine vesicular disease virus in porcine kidney cells (IB-RS-2 cells).

Crystal formation of swine vesicular disease virus (SVDV) in IB-RS-2 cells was studied by electron microscopy. Cells were harvested 0, 3, 3.5, 4, 4.5, 5, 6 and 7 hours after inoculation. Crystalline arrays of SVDV was first observed in the cytoplasm of a few cells 4.5 hours after inoculation. In the cytoplasm of many cells harvested at 5 hours, 1 to 3 crystalline arrays of SVDV were observed. After that, a small number of cells had crystalline arrays in the cytoplasm. The cells with crystalline arrays were rich in ribosome and polysome with dilated mitochondria and many tiny vesicles. An individual virus particle was ca. 18 nm in diameter, and the center-to-center space ca. 22 nm. Crystalline arrays varied in size depending on the plane of section.

Animals↗
PubMed1980

A histological and ultrastructural comparison of the sulfur granule of the actinomycosis and actinobacillosis.

The sulfur granules of actinomycosis and actinobacillosis were studied histologically and ultrastructurally. The sulfur granule of actinomycosis was large, and oval or horsehoe-shaped. In it, there were a number of gram-positive, filamentous or short rod-like hyphae beneath clubs. Ultrastructurally, the center of the sulfur granule was composed of a cluster of hyphae, and the periphery was surrounded by clubs arranged radially. The clubs were made of electron-dense fine granules and had a degenerated hypha at the center. These granules varied in amount from small to large. As for the host reaction, neutrophil infiltration appeared first around the sulfur granule. Then epithelioid cells proliferated and phagocytized hyphae. Finally, proliferating fibroblasts enclosed them and formed tiny granulomas. The sulfur granule of actinobacillosis was small and lobulated. In it, a number of gram-negative, short rod-like bacteria were present beneath clubs. Ultrastructurally, the center of the sulfur granule contained intact or degenerated bacteria, and the periphery was composed of radially projecting clubs. These clubs were made of electron-dense amorphous material, in which several layers of tubular structures surrounded the bacterium conentrically. The host reaction was almost the same as that of actinomycosis. The proliferation of fibroblasts was prominent. There was a strong tendency for these cells to form many tiny granulomas.

Actinobacillosis↗
PubMed1979

Systemic candidiasis from Candida albicans colonizing the gastrointestinal tract of mice.

Reproducible induction of systemic Candida infection was achieved by treating mice in which Candida colonization had been established in the gastrointestional tract by aminobenzylpenicillin treatment. Systemic candidiasis was induced in these mice by X-ray irradiation followed by immunosuppressive doses of dexamethasone or X-ray irradiation followed by immunosuppressive doses of trypan blue. Macrophages seem to play an important role in thie systemic infection.

Animals↗
PubMed1979

Encephalomyelitis in mice experimentally infected with Akabane virus.

Lesions in the central nervous system of mice, induced by intracerebral injection of Akabane virus, were observed by the fluorescent antibody technique and histological method. Fluorescent antigens were recognized in the cytoplasm of nerve cells, but were not detected exactly in any other part. Fluoresced nerve cells were distributed almost all over the central nervous system, especially in medulla oblongata and spinal cord. The appearance of fluorescent antigens was followed by histological changes. So-called Nissl's acute severe degeneration was observed in nerve cells in the area where the fluorescent antigens were distributed. Spongy foci were seen in medulla oblongata and spinal cord. Virus was recovered from brain and spinal cord, but not from any other visceral organ or blood. Akabane virus showed an affinity to nerve cells and caused primary nonpurulent encephalomyelitis when inoculated intracerebrally to mice.

Animals↗
PubMed1979

Pathological observation on experimental swine dysentery.

Experimental swine dysentery caused by 4 cultured strains (S73/2, DJ183, DJ70 and DK762) of Treponema hyodysenteriae was studied pathologically. The distribution and quantity of treponemes were examined on tissue sections stained by the Warthin-Starry method. Of the organs the colon contained the largest number of treponemes and the cecum and rectum the second largest number. Histopathological lesions were restricted to the large intestine. They ranged from mild catarrhal colitis in the mild case to desquamative, hemorrhagic colitis in the severest case. The severity of lesion was closely associated with the quantity of treponemes present. There was no difference in quality of the lesion between any two of the strains used in this study. Electron microscope revealed a large number of free treponemes present in the intestinal lumen and crypts. Treponemes were seen more frequently in the cytoplasm of goblet cells than in that of intestinal epithelial cells. They were also observed in desquamated degenerative epithelia. A small number of them were found in intact epithelia. Morphologically, the treponeme had a granular protoplasmic cylinder at the center which was surrounded by a thin envelope. Between the cylinder and the envelope there were axial fibrils.

Animals↗