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Biomedical subjects

S Konda

Publications and source records attributed to S Konda.

At least 91 records · Page 5Linked to original sources
PubMed1980

Non-IgM monoclonal gammopathy in patients with Sjögren's syndrome.

Two Japanese patients with Sjögren's syndrome with non-immunoglobulin M(IgM) class monoclonal gammopathy are described. The monoclonal IgA lambda detected in the serum and saliva was confirmed to possess rheumatoid factor activity in the first patient with a hypergammaglobulinemic purpura and hyperviscosity syndrome. Idiotype specificity was present on the surface membrane of peripheral blood lymphocytes as well as in the cytoplasm of infiltrating cells in the salivary glands. Common idiotypic specificity was found in four of 60 other patients who had rheumatoid factors. In the serum and saliva of the other patient, a monoclonal immunoglobulin G, kappa type (IgG kappa), was detected. Kappa type IgG was found in most of the infiltrating cells in the salivary glands and also in the saline extract from a resected submandibular gland. Our findings indicate that non-IgM class monoclonal gammopathy is also one of the complications of Sjögren's syndrome.

Female↗
PubMed1980

Sandwich radioimmunolabeling for the study of surface properties of bone marrow lymphocytes.

A modification of sandwich radioautographic method was applied to the study of surface immunoglobulin and/or specific antigens on small lymphocytes in mouse and human bone marrow. After incubation of marrow cell suspensions at 37 degrees C, cells were reacted at 0 degree C for 30 min with graded dilutions of rabbit anti-mouse or anti-human immunoglobulin followed by further reaction with a sheep anti-rabbit immunoglobulin labeled with 125I. Detectable surface immunoglobulin was demonstrated in approximately one-third of mouse marrow lymphocytes and 20-25% of human marrow lymphocytes. The densities of surface immunoglobulin as assessed by grain counts on individual labeled lymphocytes tended to be lower in the marrow than in spleen or peripheral blood. When the same rabbit antiserum was used to compared the sensitivity of the sandwich method with that of the direct radioautography, the former was found sufficiently sensitive to give a plateau level of labeling without seriously increasing background grains. The advantages of the method are discussed with reference to studies on T and B cells specific antigens on human bone marrow lymphocytes.

Animals↗
PubMed1980

The paradoxical effects of thioglycollate-induced macrophages on the growth of B16 melanoma cells in vivo and in vitro.

The effect of thioglycollate-induced macrophages on the growth of syngeneic B16 melanoma cells was studied. Thioglycollate-induced macrophages showed strong cytostasis and cytotoxicity towards B16 tumor cells in vitro. However, these effects were not demonstrable in an in vivo assay which involved local transfer and experimental metastasis. In contrast to the in vitro results, mice injected iv with both B16 tumor cells alone. Possible reasons for the failure of thioglycollate-induced macrophages to exhibit cytostatic and cytotoxic activity in vivo are discussed.

Animals↗
PubMed1978

Leukemic lymphosarcoma (LLS) with monoclonal IgM: idiotypic specificity on the cell surface and in the cytoplasm of lymphosarcoma cells.

A case of leukemic lymphosarcoma with a small amount of monoclonal IgM in the serum is described. Immunologic investigations showed that about 10% of the abnormal cells formed EAC rosettes, that most of these cells had IgMlambda on the cell surface, and that 40% of them contained IgMlambda in the cytoplasm. Monoclonal IgM was detected in the 19S fraction of the serum despite the lack of monoclonal change as determined by serum electrophoresis or immunoelectrophoresis. The common idiotypic specificity was found in the monoclonal serum IgM, surface Ig, and cytoplasmic Ig of lymphosarcoma cells using antiidiotypic antiserum prepared against purified serum IgM. After chemotherapy, cells with idiotypic determinants disappeared and the serum IgM changed from a monoclonal to a polyclonal distribution.

Adult↗