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Biomedical subjects

S Komiya

Publications and source records attributed to S Komiya.

At least 73 records · Page 4Linked to original sources
PubMed1993

Relationship of anthropometric indices of body fat to cardiovascular risk in Japanese women.

The relationship between body fat indices and cardiovascular risk was analyzed in 97 Japanese women aged 36-72 years. The variables used were anthropometric measures of body fatness, blood lipids and blood pressure. Total adipose tissue weight (TATW) was estimated from bioelectrical impedance measurements. Subcutaneous adipose tissue weight (SATW) was calculated by measuring subcutaneous fat thickness at 14 sites using a skinfold caliper. The indices of body fat distribution were the ratios of waist to hip circumference (WHR) and abdominal to hip circumference (AHR). Blood pressure and serum lipid levels were determined in all subjects after an overnight fast. The correlations among the various body fat indices were high, and all were strongly correlated with WHR and AHR; only % SATW and percentage internal adipose tissue weight (%IATW) were not correlated with WHR and AHR. Significant positive associations were found between WHR, triglycerides and systolic blood pressure (SBP). Negative associations were found between WHR and HDL-cholesterol (HDL-ch.) and the ratio of HDL-ch. to total cholesterol (HDL-ch./T-ch.). On the other hand, significant positive associations were found between AHR, T-ch., LDL-cholesterol, free fatty acid and SBP. A negative association was found between AHR and HDL-ch./T-ch.. Partial correlations were used to determine further associations between cardiovascular risk factors and fat distribution indices. SBP was associated with WHR or AHR after removing the effects of age. Similarly, HDL-ch./T-ch. was associated with WHR or AHR after removing the effects of age and degree of obesity (BMI). These results suggest that the WHR and AHR are equally valid estimates of body fat distribution with respect to their relationship with cardiovascular risk. These relationships occurred independently of age or BMI in Japanese women. These findings emphasize the importance of intra-abdominal obesity as a metabolic risk factor for cardiovascular disease associated with a high WHR and AHR.

Adipose Tissue↗
PubMed1993

[The effect of aerobic exercise training on internal body fat in obese women].

A group of 11 obese women (from 13% of ideal weight and higher) volunteered to participate in a study designed to reduce weight in a 92-day program of aerobic exercise. Before and after the weight reduction period, body fat indices were measured by deuterium oxide (D2O) dilution and by skinfold anthropometry. Changes in internal body fat and subcutaneous fat were calculated. Mean weight loss was 3.6 +/- 1.2 kg, and internal body fat and subcutaneous fat were 1.8 +/- 1.3 kg (21.2%) and 3.0 +/- 0.9 kg (16.0%), respectively. Highly significant correlation were obtained between changes in internal body fat and total body fat (r = 0.856, p < 0.001). However, changes in internal body fat were not significantly associated with changes in waist to hip ratio (WHR). In the present study, we found that changes in total body fat were significantly related to changes in internal body fat during treatment of obesity with aerobic exercise. However, changes in internal body fat were not valid estimates of changes in body fat distribution in relation to changes in WHR.

Adipose Tissue↗
PubMed1993

Simple bone cyst. Treatment by trepanation and studies on bone resorptive factors in cyst fluid with a theory of its pathogenesis.

Simple bone cysts were treated by trepanation. The technique consists of drainage of cyst fluid, lavage of the cystic cavity with saline, and the making of multiple drilling holes through the cortical and the medullary bone of the cyst wall. Injection of corticosteroid was omitted. In 11 cases treated by this method, the clinical outcome was good. Biochemical analyses of the cyst fluid showed bone-resorptive factors, i.e., prostaglandins, interleukin 1, proteolytic enzymes. Electrophoretic analysis of proteolytic enzymes in polyacrylamide gel containing sodium dodecyl sulfate and polymerized gelatin showed proteins with molecular weights of about 130,000, 92,000, 72,000, and lower than 50,000. Increase in such bone-resorbing activities seems to be one of the causative factors in simple bone cysts. The technique was effective in decompressing the internal pressure of the cysts, improving the blood flow through the medullary bone of the cyst wall, stimulating the periosteum to induce bone formation, and eliminating bone destruction.

Adolescent↗
PubMed1992

[The pathogenesis and etiology of giant cell tumor of bone from a viewpoint of bone resorptive factors].

The pathogenesis and etiology of giant cell tumor of bone was studied by analysing the bone resorptive factors in the conditioned culture medium. In the primary culture characteristic multinucleated giant cells and mononuclear cells were coexisted. The values of interleukin 1 (IL-1) and prostaglandin E2 (PGE2) in the conditioned medium obtained from the primary culture were high. In the primary culture, an immunohistochemical technique revealed the presence of IL-1 both in mononuclear cells and in giant cells. When the medium obtained from the primary culture was tested for proteolytic activity by zymography with SDS/polyacrylamide containing gelatin, multiple gelatinolytic activities were observed. In subcultures, multinucleated giant cells were not persisted and only stromal cells were visible. In subcultures, the values of IL-1 and PGE2 were much lower. Proteolytic activities were similarly weak. However, the exposure of the passaged stromal cells to the medium containing IL-1 stimulated the stromal cells to produce PGE2 and proteolytic enzymes. Immunofluorescent localization technique revealed the expression of the proteolytic enzymes in the stromal cells. These findings demonstrated that coexistence of multinucleated giant cells with mononuclear cells should be needed for the tumor to express the original phenotype. In the presence of multi-nucleated cells, mononuclear cells seem to be stimulated to produce PGE2 and proteolytic enzymes, which accelerate the bone resorption. These factors are considered to act synergetically in the resorption of bones.

Bone Neoplasms↗
PubMed1992

Rapidly destructive arthropathy of the hip. Studies on bone resorptive factors in joint fluid with a theory of pathogenesis.

The mechanism of joint destruction in rapidly destructive coxopathy was studied by analyzing bone resorptive factors in the joint fluid. Prostaglandins were found to play a partial role in joint destruction. Some cases of rapidly destructive coxopathy revealed elevated levels of interleukin-1 beta (IL-1 beta) in the joint fluid. Electrophoretic analysis of proteolytic enzymes in polyacrylamide gel containing sodium dodecyl sulfate and copolymerized gelatin demonstrated that the resorptively active peptides have relative molecular weights (M(r)) of approximately 92,000, 72,000, and lower than 60,000. Cultured cells from synovia obtained perioperatively secreted matrix metalloproteinase 2 (MMP-2) with an M(r) of 72,000 and matrix metalloproteinase 3(MMP-3) with an M(r) of 57,000. Synovial cells from the patients with coxarthrosis secreted fewer proteolytic enzymes. Prostaglandins, IL-1 beta, MMP-2, and MMP-3 could act synergetically as promotors in the rapid destruction of the hip joint.

Bone Matrix↗
PubMed1992

Cell proliferation-related production of matrix metalloproteinases 1 (tissue collagenase) and 3 (stromelysin) by cultured human rheumatoid synovial fibroblasts.

We investigated the effects of platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and insulin on the cell proliferation of and the production of matrix metalloproteinases (MMPs) by rheumatoid synovial fibroblasts in order to determine the role of these agents in rheumatoid arthritis. PDGF stimulated rheumatoid synovial fibroblasts to increase DNA synthesis and the production of precursor forms of MMP-1 of M(r) = 53,000 and -3 of M(r) = 57,000. EGF and insulin also increased DNA synthesis and the production of these enzymes, but the amount of DNA or MMPs was smaller than that induced by PDGF. Since the production of matrix macromolecules and their degradation is essential for the remodelling of synovial tissue in rheumatoid arthritis, these data suggest that the production of MMP-1 and-3 by rheumatoid synovial fibroblasts in relation to cell proliferation plays an important role in the pathological process of rheumatoid arthritis.

Arthritis, Rheumatoid↗
PubMed1992

Production of matrix metalloproteinases 2 and 3 (stromelysin) by stromal cells of giant cell tumor of bone.

Matrix metalloproteinases play a central role in the catabolism of extracellular matrix macromolecules. Here the authors report that giant cell tumor of bone (GCT) produces two matrix metalloproteinases (MMPs) in zymogen form, which have been identified as proMMP-2 (also known as "72-kDa-progelatinase/type IV procollagenase") and proMMP-3 (prostromelysin). Giant cell tumor is known to consist of two major cell populations, multinucleated giant cells and stromal cells. On several passages of the tumor cells in culture, only stromal cells proliferated. These stromal cells produced proMMP-2 but not proMMP-3. Addition of the conditioned medium of primary GCT culture or human macrophage-conditioned medium to the passaged stromal cells induced the production of proMMP-3. The production of proMMP-3 was also induced by interleukin 1 (IL-1), but not by tumor necrosis factor alpha (TNF alpha). ProMMP-1 (tissue procollagenase) was not detected even after treatment with these stimuli. Immunohistochemical studies have demonstrated that multinucleated giant cells in GCT both produce IL-1 and TNF alpha, suggesting that IL-1 secreted by multinucleated giant cells may be responsible for in vivo production of proMMP-3 by the stromal cells. The authors propose that GCT has a self-stimulatory system for the production of matrix-degrading proteinases and that the ability of the passaged stromal cells to synthesize and secrete proMMP-3 with appropriate stimuli may contribute the malignant behavior of GCT.

Adult↗
PubMed1991

[Equation for estimating total body water by bioelectrical impedance measurements in Japanese subjects].

This article reports a study in which the equation for total body water (TBW) estimated from deuterium (2H2O)-dilution method and bioelectrical impedance measurement (BIM) is described. Subjects were 60 healthy males aged 30 +/- 18.3 yr (18-74) and 31 healthy females aged 37 +/- 17.5 yr (19-70). Total body water determined by the analysis of the dilution of orally ingested deuterium oxide (1g2H2O, 99.75 atom % excess/kg body weight) in urine. Bioelectrical impedance was measured for each subjects in a supine position using an electrical impedance analyzer (500 microA, 50kHz, T-1988K, Toyo Physical Inc.) with a four electrodes (Y-250, Nihon Kohden). The mean values of total body water and the impedance in males and females subjects were 34.1 +/- 4.27 l and 25.7 +/- 2.42 l, 567 +/- 28.5 omega and 562 +/- 32.5 omega, respectively. Height squared divided by resistance (Ht2/R) correlated well with TBW as measured by 2H2 O, r = 0.530 (p less than 0.001) in males and r = 0.782 (p less than 0.001) in females. The best-fitting regression equation to predict TBW comprised Ht2/R(X1) and body weight (X2) (R = 0.915, SEE = 1.70 l in males and R = 0.834, SEE = 1.28 l in females). Equations were provided with BIM instrument for the prediction of TBW: for males TBW, l = 0.1983X1 + 0.4004X2 - 0.7938 and for females TBW, l = 0.3536X1 + 0.1269X2 + 3.3417. These results suggest that bioelectrical impedance measurement is a useful measure of total body water in Japanese subjects.

Adult↗
PubMed1991

[Comparison of methods for estimating percent body fat in distance-runners].

A comparative study was conducted between two independent methods to estimate body fat in 13 college male distance-runners (20.2 +/- 1.1 yrs) and 11 male college students (19.6 +/- 0.7 yrs) as control group. The methods dealt with different body component parameters. Body fat was estimated (1) in terms of total body water based on the analysis of dilution of orally ingested deuterium oxide (D2O) in urine, and (2) in terms of body density based on underwater weighing. The results were as follows: 1) The skinfold thickness at 14 sites in distance-runners were thinner than those in control group. The mean values for subcutaneous fat in distance-runners were 4.3 +/- 0.7 kg (7.2 +/- 1.1%), which were lower than those (8.3 +/- 2.7 kg & 13.3 +/- 3.4%) in control group significantly. Oh the other hand, the mean values for internal fat in distance-runners were 8.7 +/- 1.4 kg (14.4 +/- 1.6%), which were larger than those (6.5 +/- 3.1 kg & 10.2 +/- 4.3%) in control group. 2) The mean values for percent body fat in control group were 16.1 +/- 1.6% by skinfolds method, 20.2 +/- 5.1% by body density method and 23.5 +/- 4.6% by total body water method. The estimated values of percent body fat by the methods of body density and total body water were approximately the same. 3) The mean values for percent body fat in distance-runners were 11.9 +/- 1.4% by skinfolds method, 11.8 +/- 1.7% by body density method and 21.5 +/- 1.9% by total body water method. The estimated values of percent body fat in distance-runners were lower than those in control group.(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1991

[Methods for the assessment of human body composition: skinfold thickness and bioelectrical impedance measurements].

Research to establish indirect methods of determining human body composition began during the 1940s. Renewed interest in the assessment of human body composition has stimulated the need for a balanced understanding of available methodologies of estimating fat-free mass and fat mass. Subsequently a variety of methods has been introduced. However, attempts to describe the theory and practice of individual methods have been limited. The review summarizes the background and to describe the precision or error of skinfold thickness measurement and to highlight the strengths and the limitations of bioelectrical impedance method.

Adolescent↗
PubMed1990

Target sites of polymyxin B ototoxicity.

The present study was undertaken to determine the target sites of polymyxin B ototoxicity. This drug, at the concentration of 1 mM, was perfused through the scala tympani of the guinea pig cochlea, and cochlear microphonics and endocochlear potentials were monitored. Both cochlear potentials altered but in an independent manner. These findings indicated that not only the organ of Corti but also another tissue is involved in the ototoxicity produced. The best locus for this is the vascular stria.

Animals↗
PubMed1990

Characterization of cells cultured from human giant-cell tumors of bone. Phenotypic relationship to the monocyte-macrophage and osteoclast.

Cell culture techniques were used to clarify the histogenesis of giant-cell tumor of bone. Even after passage, nearly all of the mononuclear cells possessed tartrate-resistant acid phosphatase and receptors for eel calcitonin, which are both phenotypic markers for osteoclasts. Eel calcitonin produced an increase in the cyclic adenosine monophosphate (cAMP) content of the mononuclear cells. More than 90% of mononuclear tumor cells expressed monocyte markers; flow cytometric C3b receptor, a macrophage marker, was also detected in a few cells. These findings demonstrate that the mononuclear cells expressed phenotypes of both the osteoclast and monocyte-macrophage and that they originate in a monocyte-macrophage-osteoclast lineage. Giant-cell tumor of bone may thus provide a good model for investigating the mechanism of bone resorption in which cells of osteoclast lineage play a central role.

Biomarkers↗
PubMed1989

Relationship of the waist to hip ratio with serum lipids in women.

Recent reports have shown that a high ratio of waist to hip circumference (WHR) may be associated with a high proportion of intra-abdominal fat. The metabolic complications of obesity, which are associated with a high WHR, may therefore relate specifically to the amount of intra-abdominal fat. The purpose of this study was to determine if WHR is associated with the concentration of serum lipids that may predispose individuals to a higher risk for metabolic complications. Serum lipids concentrations were determined in 45 female subjects, ranging from 31 to 40 years in age, and WHR ranging from 0.71 to 0.98. Positive correlations of WHR with the ratio of cholesterol to HDL-cholesterol (r = 0.641, p less than 0.001) and with triglycerides (r = 0.479, p less than 0.001), were found, while a negative correlation existed between WHR and HDL-cholesterol (r = -0.600, p less than 0.001). In order to investigate the relationship between the fat distribution in the body and the serum lipids, the subjects were divided into two groups according to their % Body fat; one group with a % Body fat more than 30 and the other with a % Body fat less than 30. The two groups differed significantly in WHR. In the group of obese women with high WHR (greater than 0.87), the ratio of cholesterol to HDL-cholesterol and triglycerides were higher and HDL-cholesterol lower than in the normal group with low WHR (less than 0.80).(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1987

Interaction of polyanion against kanamycin ototoxicity.

We studied whether interference of the electron bindings between kanamycin (KM) and the outer plasma membranes of the hair cells with polyanion such as heparin can reduce ototoxicity. In a short course experiment, KM 200 mg/kg/day was injected intramuscularly 23 times with or without 1 U or 0.5 U of heparin/g/day differently to 22 guinea pigs. The reducing effect of heparin against KM ototoxicity was significant from a comparison of the N1 threshold obtained by the cochleogram. The number of surviving outer hair cells in the heparin groups was significantly greater in the third turn than in the group given KM alone. A long course experiment in which 50 injections of KM with or without 0.5 U of heparin were given intramuscularly to 16 guinea pigs revealed from the pinna reflex, cochlear microphonics and cell counting that heparin could reduce ototoxicity slightly at an early stage or before reaching crucial accumulation.

Animals↗
PubMed1986

Prognostic factors in giant cell tumor of bone. A modified histological grading system useful as a guide to prognosis.

An analysis of 26 patients with giant cell tumor of bone was carried out to determine the clinical and pathological factors affecting the prognosis of the tumor, with a follow-up program of more than 3 years. The physical characteristics of the pathological features were measured objectively using an image analyzer. A significant correlation between the therapeutic procedures and recurrence of the tumor was found, but no pathological variables were significantly correlated. Excision or resection en bloc should be performed for the initial treatment of the tumor. Local recurrence could not be predicted on the basis of histological grading. Stromal cell atypia was significantly correlated with metastasis, but multinucleated giant cells bore no correlation to either recurrence or metastasis. The use of a modified histological grading system based not on variations of the stroma and giant cells but only on the atypia of mononuclear stromal cells, either malignant or classical, is recommended for the clinical assessment of the tumor.

Adolescent↗
PubMed1985

A study on the osseous elements in malignant fibrous histiocytoma of bone.

By studying histochemically and ultrastructurally a case of malignant fibrous histiocytoma (MFH) of bone with neoplastic osteoid elements, the distinction of the tumor from osteosarcoma was discussed. MFH was likely to originate from undifferentiated mesenchymal cells that induced a histiocytic line of differentiation, and some histiocytes seemed to be transformed into well differentiated fibroblastic cells which accordingly differentiated into fibroblasts and osteoblasts. This may explain the existence of the osseous elements in MFH. Sarcoma showing the bimodal--histiocytic and fibroblastic--differentiation should be diagnosed as malignant fibrous histiocytoma, even if it contains tumorous osteoid; it must be clearly distinguished from osteosarcoma, in which the various cells only on a fibroblastic line of differentiation can be identified and the osteoblasts are the predominant cells.

Acid Phosphatase↗