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Biomedical subjects

S Kishi

Publications and source records attributed to S Kishi.

At least 163 records · Page 9Linked to original sources
PubMed1975

Studies of the clinical application of serum leucine aminopeptidase (LAP) activity determined with leucinamide as substrate.

A new method was presented for the determination of leucine aminopeptidase (LAP). The principle of the method consisted in the measurement of ammonia liberated by the action of LAP with direct colorimetry. Leucine naphthylamide has been widely used as substrate for the determination of LAP (Nap-method), but leucineamide was used (NH3-method) in this study, and the enzyme activities determined by the both methods were compared in various diseases. Serum LAP activity in acute hepatitis was much higher in NH3-method than in Nap-method, but the activity in obstructive jaundice was much higher in the latter than in the former. It was demonstrated that the serum of normal rats and CCl4 treated rats contained isozymes (LAP-I and II) which showed the different substrate specificities toward leucinamide and leucine naphthylamide. LAP-I activity was much higher, but LAP-II activity was much lower in the NH3-method than in the Nap-method. LAP-I activity was remarkably elevated by the NH3-method, but not by the Nap-method in the serum of CCl4 treated rats. The results suggested that leucinamide was preferable substrate for the measurements of activities of serum LAP in the clinical examinations.

Ammonia↗
PubMedDate not supplied

Clinical value of the guanase screening test in donor blood for prevention of posttransfusional non-A, non-B hepatitis.

We adopted an automated method for measuring guanase in donor blood and examined the incidence of posttransfusional non-A, non-B hepatitis when donor blood with high guanase activities was excluded. Sixty-seven (2.4%) of 2,826 units were excluded from use in transfusion because they had guanase activities above 1.71 units per liter. Of 112 recipients, 8 (7%) developed posttransfusional non-A, non-B hepatitis. The incidence of posttransfusional non-A, non-B hepatitis was 17% before adoption of the guanase screening test and 7% after its adoption. Thus, the incidence of posttransfusional non-A, non-B hepatitis was significantly decreased after adoption of this screening test. This study shows that, for prevention of posttransfusional non-A, non-B hepatitis, it is important to screen donor blood for guanase activity and discard blood with high activities.

Alanine Transaminase↗
PubMedDate not supplied

Serous retinal detachment in patients under systemic corticosteroid treatment.

PURPOSE: To understand the pathophysiology of central serous chorioretinopathy or bullous retinal detachment in patients under systemic corticosteroid treatment. Little is understood about the mechanism of the development of serous retinal detachment. METHODS: Three patients who had developed central serous chorioretinopathy or bullous retinal detachment during systemic corticosteroid administration were examined by fluorescein angiography and indocyanine green angiography. RESULTS: Indocyanine green angiography revealed dilated choroidal veins, delayed choroidal filling, intrachoroidal hyperfluorescence, and patchy hypofluorescence at or near the sites of dye leakage examined by fluorescein angiography. CONCLUSIONS: The primary change caused by central serous chorioretinopathy is thought to occur in the choroid, followed by the breakdown of the outer blood-retinal barrier in the pigment epithelium, resulting in the development of serous retinal detachment.

Adult↗