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Biomedical subjects

S Kishi

Publications and source records attributed to S Kishi.

At least 127 records · Page 7Linked to original sources
PubMed1983

Light and electron microscopic studies of malignant melanoma of conjunctiva and palpebra.

The authors experienced one case of malignant melanoma of the superior palpebral conjunctiva and the inferior palpebral skin, originating from benign acquired melanosis of the bulbar conjunctiva which developed in a 48-year-old man. Orbital exenteration including superior and inferior palpebrae was performed, and the removed tissue was examined by light and electron microscopy. In the temporal bulbar conjunctiva, melanocytes containing abundant fine melanin granules proliferated over almost the whole area of the epithelial layer. Cellular atypia and karyomitosis were not observed. The basement membrane remained intact with no proliferation of melanocytes in the substantia propria. However, in the perivascular area, in addition to the moderately prominent infiltration by lymphocytes and plasma cells, infiltration by melanophages was observed relatively frequently. Based on these findings, it was confirmed that the primary lesion of the bulbar conjunctiva was an acquired melanosis of Stage IB as classified by Zimmerman. The black nodular tumors of the superior palpebral conjunctiva and inferior palpebral skin consisted of melanoma cells showing markedly prominent cellular and nuclear atypia. The nuclei showed indentations in the membrane with electron-lucent karyoplasm, each having one nucleolus. The cytoplasm contained many round or elongated melanosomes in various developmental stages, with sizes of about 0.7 micron. Scattered among melanoma cells were a number of melanophages which had phagocytized many melanosomes at various stages of maturity.(ABSTRACT TRUNCATED AT 250 WORDS)

Conjunctival Neoplasms↗
PubMed1982

Structure of the inner retinal surface in simple diabetic retinopathy.

An eye with moderate simple diabetic retinopathy in a 47-year-old man was enucleated for rubeotic glaucoma of 2 months' duration and was subjected to histopathological and ultrastructural studies. Particular attention was paid to the structure of the retinal surface as examined by scanning electron microscopy. Six normal eyes and 2 eyes with long-standing central retinal vein occlusion served as the control. The inner retinal surface in normal eyes with posterior vitreous detachment appeared as a smooth, continuous sheet seen by scanning electron microscopy. No cell-like structure was detected on the retinal surface. In the eye with simple diabetic retinopathy, on the other hand, numerous spherical cells with pseudopodic protrusions were observed throughout the retinal surface which were identified as macrophages in light, scanning and transmission electron microscopic studies. The macrophages were particularly dense and were arranged in circinate pattern corresponding to circinate lesions within the retina. The macrophages were also densely populated in retinal areas with hard exudates. In areas adjacent to the macrophages, the inner retinal surface lost its smooth and continuous structure. The matrix in the inner limiting membrane disappeared in such areas so that a coarse network of filaments lay bare on the retinal surface. These findings indicate that the inner retinal surface would be pathologically altered in simple diabetic retinopathy, particularly where hard exudates are located.

Diabetic Retinopathy↗
PubMed1981

Clinical evaluation of measurement of serum guanase activity as a screening test of liver damage.

A new method was developed for assay of guanase activity by direct colorimetric determination of ammonia. In this method, dotite bicine buffer is used for preparation of a stable substrate solution and with a fixed concentration of substrate of sufficient strength serum guanase can be measured sensitively and reproducibly. This assay system could be used as a routine clinical laboratory test in the diagnosis of liver damage. GOT, GPT and guanase activities were found to be significantly elevated in patients with various liver disorders, and those with acute myocardial infarction with prominent congestion of the liver and also in CCl4- treated dogs. However, serum guanase activity was normal in patients with various other diseases, in those with acute myocardial infarction and in dogs with experimental myocardial infarction without liver damage, even when the serum GOT and GPT activities were increased. The GOT, GPT and guanase in the medium of rat hepato cytes culture with 5.0 mM CCl4 were elevated. These findings suggest that serum guanase activity is a more specific indicator of liver damage than serum GOT and GPT. The determination of serum guanase activity in patients without liver damage, even when their serum GOT and GPT levels elevated, might be useful as a screening test of liver damage.

Adolescent↗
PubMed1981

Clinical and experimental studies of the determination of serum guanase activity in acute myocardial infarction.

Serum guanase activity was measured by a new method using direct colorimetric determination of ammonia in 25 patients with acute myocardial infarction, 21 dogs with experimental myocardial infarction and 6 CCl4-treated dogs, and compared with serum GOT and GPT activity. We found normal serum guanase activity in patients with acute myocardial infarction and in dogs with experimental myocardial infarction without liver damage, even when the serum GOT and GPT activities increased. On the other hand, serum guanase and transaminase activities were elevated significantly in the patients with acute myocardial infarction who had prominent symptoms of cardiac failure and congestion of the liver and CCl4-treated dogs. These findings suggested that the serum guanase activity was more specific than serum GOT and GPT activity as an indicator of liver damage and determination of serum guanase activity in the patients with acute myocardial infarction might be useful in assessing the presence of liver impairment.

Aminohydrolases↗