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Biomedical subjects

S Katsuta

Publications and source records attributed to S Katsuta.

At least 55 records · Page 3Linked to original sources
PubMed1996

Muscle contractile activity modulates GLUT4 protein content in the absence of insulin.

We examined whether muscle contractile activity directly modulates GLUT4 protein content in rat skeletal muscle without the participation of insulin action or via amplified insulin action. To attain this purpose, the effects of increased, by training, or eliminated, by denervation, muscle contractile activity on muscle GLUT4 protein concentration were investigated in severely insulin-deficient diabetic rats. For the first set of experiments, insulin-deficient diabetic rats (induced by injection of 80 mg/kg B.W. streptozotocin) were trained for three weeks by treadmill running (90 min/day, 19 m/min, 10%, 6 days/week). GLUT4 protein concentration in soleus muscle was increased by 48% (p < 0.01) as compared with diabetic sedentary animals. For the second set of experiments, rats were injected with streptozotocin (100 mg/kg). The muscles innervated by the sciatic nerve of one leg were denervated four days after injection of streptozotocin. Three days after denervation, soleus muscles in both legs were excised. Insulin deficiency decreased GLUT4 protein concentration in innervated soleus muscle. In insulin-deficient diabetic rats, denervation also decreased soleus GLUT4 protein concentration by 50% (p < 0.01) as compared with the contralateral innervated muscle. Furthermore, the effects of insulin-deficiency and denervation on GLUT4 protein concentration were additive. These results provide evidence that muscle contractile activity directly modulates skeletal muscle GLUT4 protein concentration independent of insulin action.

Animals↗
PubMed1996

Expression of hybrid isomyosins in human skeletal muscle.

Myosin of human skeletal muscles was analyzed by means of several electrophoretic techniques. Myosin heavy chain (HC)-IIa-and HC-IIb-based isomyosins were identified by pyrophosphate-polyacrylamide gel electrophoresis (PP-PAGE). The electrophoretic mobilities of these fast-twitch muscle isomyosins differed in the order HC-IIa triplets < HC-IIb triplets. To determine the subunit composition of myosin molecules that function in intact muscle, two-dimensional electrophoresis in which the first and second dimensions were PP-PAGE and sodium dodecyl sulfate-PAGE, respectively, was also performed. Slow-twitch muscle isomyosin contained, in addition to slow-twitch light chain (LC) and HC-I isoforms, appreciable amounts of LC-2f, HC-IIa, and HC-IIb isoforms, and fast-twitch muscle isomyosin consisted of LC-2s and HC-I isoforms as well as fast-twitch LC and HC isoforms. Without consideration of HC- and slow-twitch alkali LC heterodimers, at least 31 possible isomyosins are derived from these findings on the subunit composition of isomyosins in human skeletal muscle.

Diphosphates↗
PubMed1996

Heredity of muscle fiber composition and correlated response of the synergistic muscle in rats.

The extent to which muscle fiber composition was determined by genes transmitted from parents and the correlated response of the synergistic muscle were analysed by using successive selection of rats. The foundation population (G0) was prepared by random choice from heterogeneous stock produced by random mating of three strains, Wistar-Imamichi, Fischer 344, and Donryu. Selective mating for a high percentage of fast-twitch fibers (%FT) in the deep portion of the lateral head of the gastrocnemius muscle and random mating were made from G0 to the seventh generation (G7). Successive selection caused a significant increase (P < 0.05) of %FT in the gastrocnemius muscle. The realized heritability for seven generations was 0.29. %FT in the soleus muscle of the selected line also increased (P < 0.05). In addition, significantly positive correlations (P < 0.05) were found between %FT in the gastrocnemius and soleus muscles in G1-G7. We conclude that approximately 29% of the variation of %FT was determined by genes transmitted from parents, and %FT in the gastrocnemius muscle was positively correlated with that of the soleus muscle.

Animals↗
PubMed1995

Are region-specific changes in fibre types attributable to nonuniform muscle hypertrophy by overloading?

Muscle fibre composition was compared among the proximal (25%), middle (50%) and distal (75%) regions of the muscle length to investigate whether compensatory overload by removal of synergists induces region-specific changes of fibre types in rat soleus and plantaris muscles. In addition, we evaluated fibre cross-sectional area in each region to examine whether fibre recruitment pattern against functional overload is nonuniform in different regions. Increases in muscle mass and fibre area confirmed a significant hypertrophic response in the overloaded soleus and plantaris muscles. Overloading increased the percentage of type I fibres in both muscles and that of type IIA fibres in the plantaris muscle, with the greater changes being found in the middle and distal regions. The percentage of type I fibres in the proximal region was higher than that of the other regions in the control soleus muscle. In the control plantaris muscle, the percentage of type I and IIA fibres in the middle region were higher than that of the proximal and distal regions. With regard to fibre size, type IIB fibre area of the middle and distal regions in the plantaris increased by 51% and 57%, respectively, with the greater changes than that of the proximal region (37%) after overloading. These findings suggest that compensatory overload promoted transformation of type II fibres into type I fibres in rat soleus and plantaris muscles, with the greater changes being found in the middle and distal regions of the plantaris muscle.

Adaptation, Physiological↗
PubMed1995

Nonuniform changes in fibre types in the soleus muscle of the developing rat.

Muscle fibre composition among the proximal (25%), middle (50%) and distal (75%) regions of the rat soleus muscle at various ages were compared to investigate whether the region-specific changes in fibre types known to occur under nonphysiological conditions (i.e. electrical stimulation along with immobilization in a lengthened position) also occur in the developing muscle. In addition, we attempted to detect fibres with nonuniform histochemical properties in the different segments, processing with myofibrillar actomyosin adenosine triphosphatase after pre-incubation at pH 10.3 against successive cryosections (200 microns apart). Samples were obtained from 66 Wistar rats of both sexes ranging in age from 13 to 85 days and subdivided into age groups of 2, 3, 4, 5 and 12 weeks. The mass and length of the soleus muscle increased most significantly at age 3-4 and 2-4 weeks, respectively. The distal region had a significantly lower percentage of type I fibres than the middle region at age 3 weeks, than the proximal and middle regions at age 4 weeks. In addition, some fibres [0.7 (SD 0.5)% n = 19 +/- 13] possessing nonuniform histochemical properties in different segments were observed from the middle and distal region at age 4 weeks. These findings would suggest that transformations from type II to type I fibres in the soleus muscle of the developing rat occur from the proximal or middle segments.

Aging↗
PubMed1995

Control of the rate of phosphocreatine resynthesis after exercise in trained and untrained human quadriceps muscles.

We examined the effect of differences in exercise intensity on the time constant (tc) of phosphocreatine (PCr) resynthesis after exercise and the relationships between tc and maximal oxygen uptake (VO2max) in endurance-trained runners (n = 5) and untrained controls (n = 7) (average VO2max = 66.2 and 52.0 ml.min-1.kg-1, respectively). To measure the metabolism of the quadriceps muscle using phosphorus nuclear magnetic resonance spectroscopy, we developed a device which allowed knee extension exercise inside a magnet. All the subjects performed four types of exercise: light, moderate, severe and exhausting. The end-exercise PCr: [PCr+inorganic phosphate (P(i))] ratio decreased significantly with the increase in the exercise intensity (P < 0.01). Although there was little difference in the end-exercise pH, adenosine diphosphate concentration ([ADP]) and the lowest intracellular pH during recovery between light and moderate exercise, significant changes were found at the two higher intensities (P < 0.01). These changes for runners were smaller than those for the controls (P < 0.05). The tc remained constant after light and moderate exercise and then lengthened in proportion to the increase in intensity (P < 0.05). The runners had a lower tc at the same PCr and pH than the controls, particularly at the higher intensity (P < 0.05). There was a significant correlation between tc and [ADP] in light exercise and between tc and both end-exercise PCr and pH in severe and exhausting exercise (P < 0.05). The threshold of changes in pH and tc was a PCr: (PCr+P(i)) ratio of 0.5. There was a significant negative correlation between the VO2max and tc after all levels of exercise (P < 0.05). However, in the controls a significant correlation was found in only light and moderate exercise (P < 0.05). These findings suggest the validity of the use of tc at an end-exercise PCr: (PCr+P(i)) ratio of more than 0.5 as a stable index of muscle oxidative capacity and the correlation between local and general aerobic capacity. Moreover, endurance-trained runners are characterized by the faster PCr resynthesis at the same PCr and intracellular pH.

Adenosine Diphosphate↗
PubMed1995

Muscle metabolism during exercise using phosphorus-31 nuclear magnetic resonance spectroscopy in adolescents.

Very little has been reported on muscle energetics during exercise in adolescents. This is attributable to the difficulty of subjecting children to muscle biopsy. The purpose of this study was to investigate the characteristics of muscle metabolism during exercise in vivo in adolescents by comparing firstly, with adults and secondly, the differences resulting from physical activity using phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy. The subjects were boys aged 12 to 15 years, comprising 21 trained boys and 23 control boys, and 6 adults controls. The ratio of phosphocreatine (PCr):(PCr + P(i)), where P(i) is inorganic phosphate intracellular pH at exhaustion and the time constant of PCr during recovery were measured in all the subjects using 31P NMR. Both groups of children showed higher values of PCr:(PCr + P(i)) and intracellular pH at exhaustion than did the adult control group (P < 0.01 or P < 0.05). However, no significant differences were found between the trained boys and the control boys with respect to PCr:(PCr + P(i)) and intracellular pH at exhaustion. On the other hand, we found the same values for PCr time constant in all groups. This result suggested no differences of the muscle oxidative capacity between children and adults. We concluded that the adolescents, aged 12 to 15 years in both the trained and control groups, had less glycolytic ability during exercise than the adults.

Adolescent↗
PubMed1995

Oxidative enzyme activity in the rat soleus muscle and its motoneurons during postnatal maturation.

The effect of postnatal maturation on changes in the oxidative enzyme (succinate dehydrogenase) activity in the rat soleus muscle and its motoneurons was examined at 3, 6, and 12 weeks of age. The motoneurons innervating the soleus muscle were identified using the fluorescent retrograde neuronal tracer Nuclear Yellow. An inverse relationship between soma size and oxidative enzyme activity of soleus motoneurons was observed at 3 and 6 weeks of age, whereas there was no correlation between them at 12 weeks. Although the oxidative enzyme activity in the soleus muscle increased during postnatal maturation, it showed a decrease in the soleus motoneurons. These data demonstrate that the inverse relationship between soma size and oxidative enzyme activity of rat soleus motoneurons can only be detected in the early postnatal period and that the oxidative enzyme activity in the rat soleus muscle and its motoneurons can change independently during postnatal maturation.

Age Factors↗
PubMed1994

In vivo human myocardial metabolism during aerobic exercise by phosphorus-31 nuclear magnetic resonance spectroscopy.

A few studies have been made in vivo on human myocardial energy metabolism. Hence, no discussion has taken place on metabolism during exercise or of training effects on metabolism. We examined human myocardial energy metabolism at rest and during exercise, and also training effects on the metabolism by phosphorus-31 nuclear magnetic resonance (31P NMR)-spectroscopy. Six sedentary male students (Cont) and six male long distance runners (Tr) were the subjects. Energy metabolism data were obtained from myocardium during rest and exercise by the region selection method using 31P NMR. Rotation of the legs while riding a bicycle, which was fitted with an ergometer we had made ourselves for NMR, imposed given exercise intensities. The heart rate was measured in a stationary phase during exercise. Although the heart rate at rest in the Tr group was significantly lower [Tr, 52.5 (SD 3.1) beat.min-1; Cont, 67.1 (SD 2.9) beat.min-1], no significant difference was observed in myocardial energy metabolism using the 31P NMR method [Tr, phosphocreatine/beta-adenosine 5'-triphosphate (PCr/beta-ATP); 1.51 (SD 0.02); Cont, 1.51 (SD 0.01)]. When NMR measurements were investigated at two different intensities of exercise, heart rates in the Cont group were significantly higher by about 20 beat.min-1 than those in the Tr group at both exercise intensities, while no difference in energy metabolism was observed between the groups or between rest and exercise [Tr, 75.9 (SD 3.6), 88.3 (SD 3.7) beat.min-1; PCr/beta-ATP 1.51 (SD 0.03), 1.51 (SD 0.03); Cont, 95.9 (SD 2.4), 115.1 (SD 3.5) beat.min-1, PCr/beta-ATP 1.51 (SD 0.01), 1.51 (SD 0.04)].(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine Triphosphate↗
PubMed1994

Increased capillary density due to atrophy of ischaemic soleus muscle of the rat.

This investigation was undertaken to determine whether a severe limitation of blood supply by external iliac artery ligation can change the number and density of capillaries in rat soleus muscle. The external iliac artery in one hindlimb was ligated for 2, 7 or 28 days, and the other, sham-operated, hindlimb was used as a control. Muscle blood flow in the ischaemic soleus muscle at 2, 7 and 28 days after external iliac artery ligation was significantly decreased compared with the control. The muscle fibre area and the ratio of the fibre area to body mass in the ischaemic soleus muscle at 28 days after the external iliac artery ligation were significantly reduced in the ischaemic soleus muscle, but no change in the number of capillaries per fibre was observed. Capillary density per millimetres squared at 28 days and the ratios of the number of capillaries around type I fibres to the fibre area at 7 and 28 days were significantly increased in the ischaemic soleus muscle (P < 0.05). These results suggested that long-term severe limitation of blood flow in the soleus muscle by ligation of the external iliac artery could have induced the increase in capillary density, as a result of atrophy of muscle fibres rather than capillary growth.

Animals↗
PubMed1994

Changes in magnetic resonance images in human skeletal muscle after eccentric exercise.

To investigate the time-course of changes in transverse relaxation time (T2) and cross-sectional area (CSA) of the quadriceps muscle after a single session of eccentric exercise, magnetic resonance imaging was performed on six healthy male volunteers before and at 0, 7, 15, 20, 30 and 60 min and 12, 24, 36, 48, 72 and 168 h after exercise. Although there was almost no muscle soreness immediately after exercise, it started to increase 1 day after, peaking 1-2 days after the exercise (P < 0.01). Immediately after exercise, T2 increased significantly in the rectus femoris, vastus lateralis and intermedius muscles (P < 0.05) and decreased quickly continuing until 60 min after exercise. At and after the 12th h, a significant increase was perceived again in the T2 values of the vastus lateralis and intermedius muscles (P < 0.01) [maximum 9.3 (SEM 2.8)% and 10.9 (SEM 2.2)%, respectively]. The maximal values were exhibited at 24-36 h after exercise. In contrast, the rectus femoris muscle showed no delayed-stage increase. Also, in CSA, an increase after 12 h was observed in addition to the one immediately after exercise in the vastus lateralis, intermedius and medialis and quadriceps muscles as a whole (P < 0.01), reaching the maximal values at 12-24 h after exercise. The plasma creatine kinase activity remained unchanged up to 24 h after and then increased significantly 48 h after exercise (P < 0.05). Beginning 12 h after exercise, the subjects whose T2 and CSA increased less than the others displayed a faster decrease in muscle soreness.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1993

Heredity of muscle fibre composition estimated from a selection experiment in rats.

The extent to which muscle fibre composition was determined by the genes transmitted from parents was estimated by using successive selections of rats. A foundation population (G0) was established to carry out the selections; it consisted of 100 albino rats which were randomly chosen from heterogeneous stock. The heterogeneous stock was produced by random-mating of three strains, Wistar-Imamichi, Fischer 344 and Donryu. Successive selection for a high percentage of slow twitch fibres (% ST) was made from G0 to the fourth generation (G4). The mean values of % ST changed from 50.0% in G0 to 55.6% in G4. The mean value in G4 was significantly higher than that in G0. The realized heritability in G0-G4 was calculated from the regression of selection response on the cumulated selection differential. The regression coefficient was 0.17 (SEM 0.04). The realized heritability was significantly different from 0 at the 0.05 level. We concluded that in rats about 17% of the variation of muscle fibre composition was determined by the genes transmitted from parents.

Animals↗
PubMed1993

[Muscle fiber conduction velocity in right and left biceps brachii for badminton players].

The purpose of this study was to investigate the laterality of muscle fiber conduction velocity in biceps brachii for badminton players, who have stronger arm in the handedness side for long term training. The muscle fiber conduction velocity was calculated from the propagation time of the action potentials along the muscle fibers and the electrode separation using cross-correlation method. For badminton players, significant lateral differences (e.g. right side skillful) were observed in the upper arm girth (P < 0.01) and isokinetic peak torque at 0, 10, 20, 30, 40 rpm (P < 0.01). For control subjects, significant lateral differences were in upper arm girth (P < 0.05) and isokinetic peak torque at exactly 30 rpm (P < 0.05). As for muscle fiber conduction velocity, there were no statistically significant differences between right (4.16 +/- 0.34 m/sec) and left (4.08 +/- 0.34 m/sec) sides in badminton players, and the control group exhibited same tendency (right: 4.36 +/- 0.25 m/sec, left: 4.28 +/- 0.33 m/sec) as well. These results suggest no training-induced change in the muscle fiber conduction velocity.

Adult↗
PubMed1993

[A case of pulmonary malignant lymphoma with macroglobulinemia and cecal cancer].

A 69-year-old female was found to have an abnormal shadow in the right middle lobe during examination for operation for cecal cancer. Ten months later, she was admitted to our hospital because of enlargement of the shadow and development of macroglobulinemia. Transbronchial lung biopsy and elevated IgM in BALF (IgM/Alb was 2.75 times that in serum evidently suggested pulmonary malignant lymphoma. CHOP therapy was not effective, and resection of the mass was attempted but was not feasible because of extension of the lesion into the upper and lower lobes. The biopsy specimen obtained during surgery disclosed malignant lymphoma, diffuse small cell type (IgM kappa type, B-cell). Radiation therapy was effective with complete resolution of the shadow. In this case, transbronchial lung biopsy and BAL were useful in the preoperative diagnosis of pulmonary malignant lymphoma.

Aged↗
PubMed1993

The effect of nutrition on the size and proportion of muscle fibre types during growth.

To investigate whether the retardation in the increase of body weight produced by reduced food intake could influence the transformation of muscle fibre types in soleus and extensor digitorum longus (EDL) during growth, rats were divided into 3 groups at 3 wk of age. Each group was subjected to food restriction from 3 wk of age to the following ages. Group 1 comprised 5 (148.7 +/- 7.3 g), 7 (250.7 +/- 11.1 g), 9 (362.9 +/- 19.3 g) and 11-wk-old rats (414.9 +/- 35.2 g) fed ad libitum. Group 2 comprised 5 (148.7 +/- 7.3 g), 7 (148.6 +/- 7.7 g), 9 (147.7 +/- 6.0 g) and 11-wk-old rats (148.8 +/- 5.7 g) fed a restricted diet; these animals were similar in weight to the 5-wk-old rats in group 1. Group 3 comprised 4 subgroups of 11-wk-old rats (148.7 +/- 5.7 g, 247.6 +/- 6.8 g, 354.4 +/- 8.6 g, 414.4 +/- 35.2 g); their body weights were adjusted to the weights of 5, 7, 9 and 11-wk-old rats in group 1 by restriction of food intake. Muscle weights and fibre areas in soleus and EDL significantly increased with growth. The muscle weights and fibre areas in group 2 in which body weights were equal increased significantly with age, but the increases were significantly less than for group 1. The muscle weights and fibre areas in group 3 in which ages were equal increased significantly with increasing body weight; the increases were the same as those in group 1.(ABSTRACT TRUNCATED AT 250 WORDS)

Aging↗