Biomedical subjects

S Kasai

Publications and source records attributed to S Kasai.

At least 73 records · Page 4Linked to original sources

PubMed1998

Molecular cloning and nucleotide sequence of a cytochrome P450 cDNA from a pyrethroid-resistant mosquito, Culex quinquefasciatus say.

A novel cDNA clone encoding a cytochrome P450 was screened from a gut cDNA library of permethrin-resistant Culex quinquefasciatus larvae. Nucleotide sequence of the clone designated CYP6E1 was determined. This is the first full-length sequence of a mosquito P450 cDNA. Comparison of the deduced amino acid sequence of CYP6E1 to those of other known cytochrome P450s indicates that this clone belongs to CYP6 family. In addition, a phylogenetic analysis of CYP6E1 primary structure showed that it is related to CYP6A and CYP6C subfamilies. Polymerase chain reaction (PCR) using primers designed on the basis of the nucleotide sequence of the cDNA showed that mRNA encoding CYP6E1 is present in permethrin-susceptible larvae as well as -resistant larvae, suggesting the ubiquitous distribution of CYP6E1.

Amino Acid Sequence↗

PubMed1998

A case of a human-papillomavirus-60-induced wart with clinical appearance of both pigmented and ridged warts.

Human papillomavirus (HPV) type 60 infection is histologically associated with characteristic homogeneous intracytoplasmic inclusion bodies. However, it remains unclear whether the virus is associated with cystic, pigmented or ridged plantar warts. We report a 51-year-old Japanese female with a HPV-60-induced plantar wart which showed the clinical appearance of both pigmented and ridged warts. Masson-Fontana staining revealed increased melanin granules in the epidermis of the wart. This observation suggests that HPV-60 may be associated not only with cystic warts but also with the specific morphology of ridged warts, and the biological disorder of hyperpigmentation may be controlled by additional unknown factors which differ from case to case.

Female↗

PubMed1998

Pelvic nerve grafts restore bladder function in denervated rats.

BACKGROUND: Autonomic nerve preservation techniques for use during surgery for rectal cancer have improved. Nevertheless, in some patients pelvic nerves must be sacrificed to excise all tumor. For these patients, nerve reconstruction at the time of operation by using nerve grafting would be useful. A rat model of this type of nerve reconstruction is described. METHODS: Animals were divided into three groups. In the sham control group, a pelvic exploration was conducted without division of the pelvic nerves. In the nerve ablation group, both pelvic nerves were excised segmentally. In the graft group, both pelvic nerves were excised and genitofemoral nerves were interposed bilaterally. At 2-week intervals postoperatively, animals from each group underwent cystometry under urethane anesthesia and neuronal tracing using fragment C of tetanus toxin for demonstration of axonal transport via regenerated nerves. RESULTS: At 6 weeks postoperatively, 60% of the grafted animals produced rhythmic contractions of the bladder. In neuronal tracing studies at weeks 4 and 6, respectively, 40% and 100% of the nerve-grafted rats had labeled neurons in the sacral parasympathetic nucleus. CONCLUSIONS: These findings suggest that pelvic nerve grafting in rats can successfully restore bladder function after surgical injury. Clinical use of pelvic nerve grafting may be indicated for patients whose pelvic nerves must be sacrificed to excise all tumor.

Animals↗

PubMed1998

Allogeneic hepatocyte transplantation: contribution of Fas-Fas ligand interaction to allogeneic hepatocyte rejection.

Hepatocyte transplantation is a potential therapeutic modality for overcoming the shortage of liver donors, and the clinical application of allogeneic hepatocyte transplantation has been considered. However, there are two major problems with allogeneic hepatocyte transplantation: protection of transplanted hepatocytes from rejection and stimulation of the rapid proliferation of surviving cells. Without immunosuppression, allogeneic hepatocytes are rapidly rejected within a few days after transplantation, even though it is relatively easy to induce immunotolerance after allogeneic whole liver transplantation. Accordingly, different rejection mechanisms seem to operate after allogeneic hepatocyte transplantation and whole liver transplantation. To overcome the rejection of transplanted hepatocytes, induction of donor-specific unresponsiveness to graft without compromising the host immune system would be ideal. We previously reported that the Fas-Fas ligand system plays a critical role in the CD28-independent pathway of hepatocyte rejection. Therefore, blockade of rejection using CTLA4 immunoglobulin (CTLA4Ig) or anti-CD80/86 monoclonal antibodies and anti-FasL monoclonal antibody may prolong the survival of transplanted allogeneic hepatocytes. Furthermore, administration of hepatocyte growth factor (HGF) can promote the proliferation of allogeneic hepatocytes and this may lead to the development of a functioning liver substitute.

Animals↗

PubMed1998

[Factors associated with participation in chest X-ray screening by young and middle aged residents].

We investigated the factors that affect participation rates in chest X-ray mass screenings among 20-59 year-old residents in A city, Kagawa prefecture. 1) Participation rates in employees and non-employees were 78.3% and 33.9% respectively. In the employees whose firms do not provide health check-ups, participation rate was 15.9%. 2) Among participants, about 90% of the employees whose firms do not provide health check-ups and about 60% of non-employees utilized mass screenings provided by the local government. 3) Among non-participants, 53.7% of the employees whose firms do not provide health check-ups and 70% of the others (employees whose firms provide health check-ups and non-employees) were aware of the screening system. Most of the young did not know of the system. 4) Principal reasons for nonparticipation were "inconvenient" and "unnecessary because healthy" in job holders and "unnecessary because healthy" in non-job holders. 5) Participants had better health practices than non-participants. Participation rates of chest X-ray mass screenings were high in employees and low in non-employees. Local governments are expected to supplement the existing mass screening system at work-sites by providing accessibility screening. In tuberculosis prevention, nonparticipation in mass screenings is one of the main issues. The association between participation and health practice suggests that methods for linking with primary prevention activities are necessary for stimulating interest and promoting participation in mass screenings.

Adult↗

PubMed1997

Enhancement of proliferation of intrasplenically transplanted hepatocytes in cirrhotic rats by hepatic stimulatory substance.

This study aimed at investigating the efficacy of hepatic stimulatory substance (HSS) on the proliferation of transplanted hepatocytes in the spleen of rats with carbon tetrachloride (CCl4)-induced liver cirrhosis. After hepatocyte transplantation (HTx; 1x10(7) cells/rat), the recipients received intravenous administration of HSS (3ml) of 2, 4, or 7 times/week to investigate the effect of the frequency of HSS treatment on the proliferation of intrasplenically transplanted hepatocytes. Next, to investigate the effect of the severity of liver cirrhosis on HSS-stimulated proliferation of transplanted hepatocytes, different doses (0.00, 0.08, 0.12, and 0.16 ml/week) of CCl4 were given to rats after HTx. The recipients were killed and the spleens were removed at 2 and 4 weeks after HTx and stained with bromodeoxyuridine (BrdU) and hematoxylin and eosin for determining the BrdU labeling index (L.I.) and the hepatocyte-occupied area ratio in the longitudinal cut surface of the spleen (H:S ratio), respectively. The H:S ratio was measured in the hematoxylin and eosin-stained splenic sections under a light microscope connected to an image processor. HSS-treated rats showed significantly higher BrdU L.I. and H:S ratios than the untreated control rats at 2 and 4 weeks after HTx. However, the difference in the BrdU L.I. and H:S ratio was not statistically significant among the HSS-treated rats. Without HSS treatment, the severity of liver cirrhosis did not affect the proliferation of intrasplenically transplanted hepatocytes. On the other hand, HSS-induced proliferation of transplanted hepatocytes was further enhanced in proportion to the severity of liver cirrhosis. The H:S ratio of the rats treated with 0.04, 0.08, and 0.16 ml/week of CCl4 after HTx was 1.27+/-0.5%, 4.32+/-0.65%, and 6.25+/-0.70%, respectively, at week 4. During the long-term observation of up to 12 weeks, a marked decrease in the H:S ratio was observed in HSS-untreated control rats at weeks 4-12 compared with week 2. While HSS-treated rats revealed gradual proliferation of intrasplenically transplanted hepatocytes, the difference in the H:S ratio between HSS-treated and -untreated rats became larger. These results indicate that HSS treatment of recipient rats stimulated the proliferation of intrasplenically transplanted hepatocytes in cirrhotic rats and that the severity of liver cirrhosis enhanced the stimulative effect of HSS on the proliferation of transplanted hepatocytes.

Animals↗

PubMed1997

Functional assessment of proliferating hepatocytes stimulated by hepatic stimulatory substance in ascorbic acid biosynthetic enzyme-deficient rats.

The functional ability of hepatic stimulatory substance (HSS)-stimulated proliferating hepatocytes was investigated by intrasplenic and/or intraportal transplantation in ascorbic acid (AsA) biosynthetic enzyme-deficient (ODS-od/od) rats that die of osteogenic disorders unless there is AsA supplementation. HSS was extracted from regenerating porcine livers. Hepatocytes isolated from the livers of congeneic ODS-+/+ rats that are capable of synthesizing AsA were transplanted into the spleen (Sp-HTx) and/or the portal vein (Pv-HTx) of ODS-od/od rats. The recipients were divided into eight groups as follows: HSS-untreated groups [group Ia, sham-operated, HTx(-); group IIa, Sp-HTx; group IIIa, Pv-HTx; and group IVa, Sp- and Pv-HTx], HSS-treated groups [group Ib, HSS only; group IIb, Sp-HTx + HSS; group IIIb, Pv-HTx + HSS; and group IVb, Sp- and Pv-HTx + HSS]. The recipients were given a diet and water containing AsA for 6 weeks after HTx, and AsA supplementation was then halted. The average bromodeoxyuridine (BrdU) labeling index (LI) and hepatocyte-occupied ratio in the spleen (H/S ratio) of HSS-treated rats were significantly higher than those of HSS-untreated rats. All the rats in HSS-untreated groups and group Ib died by 8 weeks after the cessation of AsA. In HSS-treated groups IIb, IIIb, and IVb, the survival rates were 60%, 50%, and 80%, respectively, at 16 weeks after HTx. The average serum AsA level of the surviving rats in groups IIb, IIIb, and IVb was significantly higher than that in HSS-untreated groups. These results indicate that HSS treatment induced rapid proliferation of transplanted hepatocytes in the spleen and the portal vein, and that these proliferating hepatocytes synthesized AsA and improved the survival rate of ODS-od/ od rats.

Animals↗

PubMed1997

Orthotopic transplantation of a partial hepatic autograft in dogs.

The purpose of this study was to investigate the availability of an orthotopic transplantation of partial hepatic autograft in dogs as a means of surgical training. Male mongrel dogs weighting 10-15 kg were used. The left lobe of the liver was harvested while preserving the left branches of the portal vein, hepatic artery and bile duct, and the left hepatic vein. The remnant liver was removed while preserving the inferior vena cava using a veno-venous bypass. Orthotopic transplantation of the autograft was performed while anastomosing the left hepatic vein to the inferior vena cava, portal and arterial reconstruction, and external biliary drainage. Thirteen out of 29 dogs survived more than 48 h after transplantation. However, 6 out of 13 dogs were sacrificed after developing bile peritonitis due to a dislodgement of the biliary catheter, and only two dogs were able to survive for 7 days after transplantation. The arterial ketone body ratio recovered to 1.0 within 1 h after reperfusion, and the ratio of the dogs that survived for more than 48 h remained above 1.0 until sacrifice. Orthotopic transplantation of a partial hepatic autograft is a useful and simple procedure to train surgeons for partial liver transplantation.

Animals↗

PubMed1997

Successful treatment of a rectal anastomotic stenosis by transanal endoscopic microsurgery (TEM) using the contact Nd:YAG laser.

We report the advantage of employing transanal endoscopic microsurgery (TEM) using the contact Nd:YAG laser for the treatment of a rectal anastomotic stenosis. A 72-year-old woman was admitted to our hospital with a postoperative rectal anastomotic stenosis. Twenty months prior to admission, the patient underwent a low anterior resection for the treatment of the rectal cancer using an EEA stapling device. A barium enema and colonoscopy revealed a rectal stenosis, 0.8-cm diameter. This stenosis was at the anastomotic site, approximately 4.0 from the dental line. An endoscopic treatment was performed transanally using the contact Nd:YAG laser. The stenotic rectal wall was fulgurated or vaporized completely. There were no intraoperative or postoperative complications. We concluded that TEM appears to be a safe and minimally invasive procedure. Furthermore, the contact Nd:YAG laser is very effective in treating the gastrointestinal stenotic area. To our knowledge, this is the first successful report of this novel procedure.

Aged↗

PubMed1997