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Biomedical subjects

S Kano

Publications and source records attributed to S Kano.

At least 307 records · Page 17Linked to original sources
PubMed1980

Stimulation of human lymphocyte subpopulations by protein A from Staphylococcus aureus.

Protein A from Staphylococcus aureus is known to stimulate human lymphocytes. Using 3H-thymidine incorporation, virus plaque assay and induction of cytotoxic T lymphocytes (CTL), this study showed that soluble or insoluble protein A stimulated different lymphocyte subpopulations. Soluble protein A is highly mitogenic to T lymphocytes. In both 3H-thymidine incorporation and virus plaque assay, its maximum stimulation was as high as the stimulation by the nonspecific mitogens phytohemagglutinin and concanavalin A, and a higher CTL response than that induced by phytohemagglutinin or concanavalin A was induced. Mitogenic activity to B lymphocytes was negligible. S. aureus (Cowan I strain) is itself considered to be an insoluble form of protein A and is 3--4 times more mitogenic to B lymphocytes than pokeweed mitogen without any increase in virus plaque-forming cells. No mitogenicity was noted to T lymphocytes. Sepharose CL-4B-protein A, also known as insoluble protein A, stimulated both T and B lymphocytes effectively, but its mitogenicity to T lymphocytes was considered to be due to the soluble protein A released from the Sepharose CL-4B beads.

B-Lymphocytes↗
PubMed1980

Activation of secondary cytotoxic lymphocytes by cell-free factors from I-region-primed and D-region-primed lymphocytes.

Cell co-operation in the generation of secondary cytotoxic responses was studied by selectively sensitizing lymphocytes in mixed lymphocyte culture (MLC) across I or D region difference and by combining the primed lymphocytes in the secondary MLC. Secondary cytotoxic responses were induced in D-region-primed lymphocytes by restimulation with the original priming D-region antigens, by co-culturing with the I-region-primed lymphocytes in the presence of the priming I-region antigens, or by cell-free supernatants obtained 24 h after the restimulation of D-region-primed lymphocytes and I-region-primed lymphocytes, The active MLC supernatants produced by both I-region-primed and D-region-primed cells also induced accelerated proliferative responses in D-region-primed lymphocytes. Heat-treatment or ultraviolet irradiation of the stimulator cells eliminated the capacity of the cells to induce the production of CTL-helper factor in I-region-primed and D-region-primed lymphocytes. It was concluded that both I-region-primed and D-region-primed lymphocytes produce a cell-free factor which induces proliferation and secondary cytotoxicity in D-region-primed lymphocytes. The possible participation of D-region reactive helper T cells and D-region reactive cytotoxic T cells in the cytotoxic responses to D-region antigens in the absence of I-region difference is discussed.

Animals↗
PubMed1980

Generation of autoreactive cytotoxic lymphocytes by spontaneously established autologous lymphoblastoid cell line.

Using spontaneously established autologous and allogeneic LCL as stimulator and target cells, we studied the generation of autoreactive cytotoxic T lymphocytes (CTL) in primary and secondary autologous MLC. In the primary autologous MLC, cytotoxic activity was detected on the 6th day and was found to be specific to autologous LCL. In the secondary autologous MLC, cytotoxic activity was detected earlier on the 3rd day, and was at a higher level than in the case of the primary autologous MLC. This cytotoxic activity was induced in both the primary and the secondary autologous MLC when T lymphocytes were used as responder cells. When autologous LCL-primed lymphocytes were used as responder cells in the secondary MLC, autologous LCL-specific CTL were not only generated by autologous LCL but also by allogeneic LCL, and the autologous LCL-specific CTL induced by allogeneic LCL was thought to be due to the nonspecific helper effect of allogeneic LCL-stimulation.

Animals↗
PubMed1980

Augmentation of mouse natural killer cell activity by a streptococcal preparation, OK-432.

Streptococcal immunopotentiator OK-432 (NSC-B116209) augmented the natural killer (NK) cell activity of peritoneal exudate cells (PEC) in inbred C57BL/6 mice given ip injections of 0.1 mg OK-432 per mouse. The cytotoxic activity of PEC increased as early as 1 day after inoculation, reached its peak on day 3, and gradually declined thereafter, YAC-1, K562, and MOLT-4 target cells were more sensitive to PEC than were EL 4 and P815 target cells. The elimination of adherent cells by a nylon wool column enriched the proportion of cytotoxic cells among PEC. Nylon wool column-passed PEC were resistant to treatment with anti-Thy 1.2 antibody plus complement and sensitive to anti-asialo GM1 serum plus complement. Because 1:40-diluted rabbit antiserum against glycosphingolipid asialo GM1 is capable of eliminating mouse NK cell activity and is not cytotoxic to killer T-cells, the above results strongly suggest that OK-432 augments the NK cell activity in mice.

Animals↗
PubMed1980

Effects of corticosteroids on natural killer cell activity in systemic lupus erythematosus.

The effects of corticosteroids on the natural killer (NK) cell activity of human peripheral blood lymphocytes were evaluated. When six untreated female patients with systemic lupus erythematosus were compared with fifteen age-matched, corticosteroid-treated female patients, NK activity in the latter was significantly suppressed. Although the administration of high doses seemed to suppress cytotoxicity to a greater extent, there was no close correlation between the daily doses of steroids and NK activity. When cytotoxicity levels were followed before and during corticosteroid therapy in the same patient, NK activity decreased markedly during treatment, particularly in patients on high-dose corticosteroids.

Adult↗
PubMed1979

A case of multiple extra-adrenal pheochromocytomas.

A 22 year old man with two extra-adrenal pheochromocytomas located in the mediastinum and in the pelvis is presented. We believe this is the first report in Japan of extra-adrenal multiple pheochromocytomas in adult located in the two distant places.

Adult↗