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Biomedical subjects

S K Kang

Publications and source records attributed to S K Kang.

132 records · Page 8Linked to original sources
PubMedDate not supplied

Neurobehavioral changes in shoe manufacturing workers.

A study was carried out to evaluate: (a) the value of a cumulative exposure estimate (CEE) calculated from limited information on air concentration; and (b) whether chronic exposure to mixed organic solvents at a shoe manufacturing factory has induced neurobehavioral changes. The Neurobehavioral Core Test Battery was administered to 40 female workers from a shoe manufacturing factory and 28 housekeepers as referents. The airborne concentrations of the solvents as a mixture were 0.46-0.71 at the frame making process and 1.83-2.39 at the adhesive process. Performance was analyzed by three exposure indices: current department, exposure duration, and CEE. The Santa Ana Dexterity test showed statistically significant differences only in CEE. In particular, the group with higher exposures had a significantly poorer performance than the reference group, on preferred hand trials. The results suggested that CEE appeared to be a better indicator of mixed organic solvents exposure, and neurobehavioral changes were associated with CEE over 10 years.

Adult↗
PubMedDate not supplied

The effect of PDE5 inhibition on the erectile function in streptozotocin-induced diabetic rats.

The aim of this study was to assess the effect of phosphodiesterase 5 inhibitor, DA-8159, on erectile function throughout the quantitative analysis of vascular endothelial cell, smooth muscle (SM), TGF-beta1 expression in rat corpus cavernosum and measurement of intracavernous pressure (ICP) in diabetic rats. DA-8159 (0, 5, 10, 20 mg/kg) was administered orally once a day to diabetic rats. After 8 weeks, immunohistochemistry and computerized image analysis were performed to quantify the percent area within the Corpora Cavernosa occupied by the endothelial cells, SM cells and fibrotic tissues. ICP/mean arterial pressure (MAP) was also measured by electrostimulation of the cavernous nerve. Diabetic rats showed a significant decrease in the SM and endothelial cell content, and an increase in the TGF-beta1 expression level within the cavernosa areas compared to the normal rats. The mean cavernous SM, endothelial cell content and TGF-beta1 expression level were 9.7+/-0.7, 4.5+/-0.7 and 17.9+/-2.1%, respectively. DA-8159 prevented reduction of SM (12.3+/-0.4% (5 mg/kg), 13.8+/-0.4% (20 mg/kg)) and endothelial cell content (5.6+/-0.5% (5 mg/kg), 6.3+/-0.6% (20 mg/kg)). Immunoreactivity of TGF-beta1 and intracorporal fibrosis were also significantly lower in DA-8159-treated groups (11.8+/-1.2% (5 mg/kg), 9.5+/-1.1% (20 mg/kg)). Electrostimulation of the cavernous nerve induced significant increase in maximum ICP (62.2+/-13.6 mmHg in 10 mg/kg vs 37.5+/-17.5 mmHg in diabetic group) and area under the curve of the ratio of ICP/MAP (8891.09+/-1957 in 10 mg/kg vs 6315.87+/-2272 in diabetic group). These results suggest that subchronic treatment of DA-8159 can prevent the development of erectile dysfunction (ED), and provides a rationale for the use of DA-8159 as treatment of diabetic ED.

3',5'-Cyclic-GMP Phosphodiesterases↗
PubMedDate not supplied

Comparison of one-step RT-PCR and a nested PCR for the detection of canine distemper virus in clinical samples.

OBJECTIVE: To develop a rapid and sensitive method for the detection of canine distemper virus (CDV) by nested PCR using clinical specimens. DESIGN: A nested PCR was developed, compared to a one-step RT-PCR and validated. PROCEDURE: Two sets of specific primers for a one-step RT-PCR and a nested PCR, targeting a 640 bp fragment and a 297 bp fragment, respectively, were selected from the highly conserved region of the nucleocapsid protein (NP) gene of CDV. The nested PCR and the one-step RT-PCR were used to amplify a part of the CDV NP gene of a CDV vaccinal strain and samples of urine, blood, nasal discharge and saliva from 29 dogs suspected of suffering CD. RESULTS: Both the one-step RT-PCR and the nested PCR reacted with the CDV vaccinal strain, but not with canine parvovirus. The expected 640 bp fragment of the NP gene was detected in 11/22 (50.0%) blood, 10/20 (50.0%) urine, 5/25 (20.0%) saliva and 6/27 (22.2%) nasal swab samples by one-step RT-PCR, whereas the nested PCR amplified an expected 297 bp fragment of the NP gene in 18/22 (81.8%) blood, 15/20 (75.0%) urine, 14/25 (56%) saliva and 19/27 (70.3%) nasal swab samples. CONCLUSION: The nested PCR detected CDV in blood, urine, nasal swab and saliva more frequently than did the one-step RT-PCR. Therefore, this assay should be a useful aid to antemortem diagnosis of CDV infections in dogs.

Animals↗
PubMedDate not supplied

Improved marginal seating of cast restorations using a silicone disclosing medium.

This study was conducted to determine the effectiveness of a silicone disclosing medium, G-C Fit-Checker, as an aid in the improvement of marginal integrity. Standardized preparations were made on 42 freshly extracted premolars. Base metal alloy castings were finished externally and initially adjusted internally. The castings were placed on their respective teeth and the marginal opening was determined. They were then adjusted twice using the silicone material and remeasured. The preadjustment mean marginal opening was 92 microns and the postadjustment measurement 55 microns, yielding a mean reduction of 37 microns (SEM 3 microns) or 39%.

Bicuspid↗
PubMedDate not supplied

Species, strain and sex differences in susceptibility to gamma radiation combined with diethylnitrosamine.

Our previous data suggested that in Sprague-Dawley (SD) rats, a short-term in vivo radiation carcinogenesis model can be established using glutathione S-transferase placental form (GST-P) positive hepatic foci induced by diethylnitrosamine (DEN) after gamma radiation. In the present study, differences between species, strain and sexes in susceptibility to radiation or radiation combined with DEN were examined. Newborn Fischer 344 (F344) and SD rats, and N:GP(S) mice were irradiated with whole body single 3Gy of gamma rays with or without intraperitoneally-injected DEN (15 mg/kg body weight) within 1 h of irradiation. Three weeks later, 0.05% phenobarbital was given in drinking water as a promoter, and at week 21 after birth, all animals were sacrificed. In both the radiation alone and radiation combined with DEN groups, F344 rats were more susceptible than SD rats to the induction of GST-P+ foci. In F344 rats, no significant difference in foci induction was seen between the sexes in both radiation alone and radiation combined with DEN groups, but in SD rats, female rats were more susceptible than males. In the case of mice, lung tumor incidence was examined, and in the radiation alone group, no differences between the sexes were seen; in the radiation combined with DEN group, however, females were more susceptible than males. These results suggested that susceptibility to radiation carcinogenesis could be potentiated by chemical carcinogens and showed wide variation between species, strains and sexes, of experimental animals.

Animals↗