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Biomedical subjects

S K De

Publications and source records attributed to S K De.

51 records · Page 3Linked to original sources

The influence of ultrasonic waves alone and in presence of certain salts on the microbial production of alcohol by Saccharomyces cerevisiae.

It was, thus, concluded that the influence of ultrasonic waves on the production of alcohol in presence of NaCl was inconspicuous as compared with the effects of other salts. It was evident from the fact that even the optimum yield of alcohol could not exceed the control. However, the influence of ultrasonic waves alone and its influence in presence of KH2PO4 could enhance the amount of alcohol over the control by 45.6% and 26.1%, respectively. The best result obtained amongst these treatments was found with the effect of sonics in presence of NH4Cl, in which the amount of alcohol produced was greater than that of the control by 51.3%.

Ammonium Chloride↗

Virus excretion in smallpox. 1. Excretion in the throat, urine, and conjunctiva of patients.

Excretion of virus in the throat, urine, and conjunctiva of smallpox patients was studied daily for 2-3 weeks after the onset of fever. The virus titre in the throat and urine of haemorrhagic and confluent cases was higher than in discrete cases. The duration of virus excretion was also greater in confluent cases than in discrete cases. Conjunctival swabs from all 12 smallpox patients with conjunctivitis were positive for virus. The duration and titre of virus excretion in the throat, urine, and conjunctiva of patients were not related to their age or sex and did not depend on whether or not they had received a primary vaccination.

Adolescent↗

Virus excretion in smallpox. 2. Excretion in the throats of household contacts.

Throat swabs of 34 of 328 family contacts of 52 smallpox cases, examined 4-8 days after the onset of the disease in the family, were positive for variola virus. The log titre of virus per swab ranged from 2 to 3.95. A higher proportion of unvaccinated than of vaccinated contacts excreted the virus. Only 4 of the virus-positive contacts developed clinical smallpox; this occurred 5-7 days after their swabs were examined. Excretion of virus in the throats of these contacts, a few of whom were in the incubation period of the disease, suggests the possibility that they could have spread the infection. This possibility, if kept in mind, may help in tracing the source of infection or in determining the incubation period in a few instances when difficulty is experienced.

Adolescent↗

Thoracic kidney.

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Adult↗

Mouse and rat placental cell lines express abundant amounts of laminin.

Placental cell lines derived from midgestation placentae of outbred mice and rats were evaluated for the expression of the extracellular matrix protein laminin. The murine cell line, which has not been previously reported, demonstrates morphological characteristics similar to those of the rat cell line. Placental cell lines grow vigorously both in vitro and when transplanted to the peritoneum of allogeneic hosts. When transplanted, placental cells form cyst-like structures (with acellular cores) suspended in the peritoneal fluid, and invade abdominal structures forming solid masses. Using immunohistology, laminin was identified within in vitro cultured cells, within cyst-like structures and their acellular cores, and as a major component of the extracellular matrix of solid masses. Laminin was also identified in the normal rat chorioallantoic placenta. Evaluation of extracts from in vitro cultured placental cells, transplanted placental cells, and the normal chorioallantoic placenta by electrophoresis and immunoblotting demonstrated that laminins were composed of two species with molecular weights of 400,000 (A-chain) and 200,000 (B-chains). Mouse and rat placental cell lines may be valuable for studying laminin biosynthesis and function in the developing placenta.

Animals↗