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Biomedical subjects

S K Clarke

Publications and source records attributed to S K Clarke.

At least 37 records · Page 2Linked to original sources
PubMed1976

Comparison of human fecal and serum parvo-like viruses.

Parvovirus-like particles found in the sera of two blood donors had the size and appearance on electron microscopy of a virus (B19) found in the serum of a blood donor by Cossart et al. (1975), and those of a virus found in the feces of a normal subject. Antibody to these viruses was detected by immune electron microscopy and immunoelectro-osmophoresis in the sera of 50 children aged 10 to 15 years. Of these, 36% had antibody to the fecal virus, 36% had antibody to B19, and 54% had antibody to the two other serum viruses. The results of these tests suggest that serologically the three serum viruses were similar to one another, but that the fecal virus was distinct. The two blood donors had nonspecific symptoms at the time of viremia. Both donors had developed immunoglobulin M antibody to the virus when tested 3.5 and 4.5 weeks later, but no viruses were detected in the feces or urine.

Adolescent↗
PubMed1975

Coronavirus propagated from patient with non-bacterial gastroenteritis.

A faecal suspension from a patient with gastroenteritis, which contained large numbers of coronavirus particles when examined by electron microscopy, was inoculated into human embryo intestinal-organ cultures and primary human embryo-kidney monolayers. In the organ cultures, the villous epithelium became detached and large numbers of coronavirus particles were seen by electron microscopy both in the medium and in thin sections of the intestinal epithelial cells, where the virus appeared to be multiplying. In organ cultures and in human embryo-kidney cells, intracytoplasmic virus was demonstrated by indirect immunofluorescence with convalescent serum from the patient.

Coronaviridae↗
PubMed1974

A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanol.

A simplified method for the detection of rubella-specific IgM in sera was developed involving HAI tests on only one fraction from a sucrose density gradient with and without treatment with 2-mercaptoethanol (2 ME). Slight trailing of IgG into the IgM fraction was shown to occur in patients with high titres of both IgG and IgM, but this could be detected by the 2ME treatment and did not affect the results.By this test, rubella-specific IgM was found in all of 54 patients with a rash and a rise of rubella HAI antibodies. When the antigen and serum were incubated for 1 hr. before adding the RBC, specific IgM was found in all 51 sera taken between 5 and 40 days after onset, and in about half the sera taken between 42 and 77 days, but in none of 16 people known to have possessed rubella antibodies at least a year previously. When antigen and serum were incubated for 18 hr. before adding the RBC, specific IgM was found in all of 21 sera taken between 2 and 49 days after onset, and in 6 of 11 sera taken between 50 and 117 days, but not in 9 people known to have possessed rubella antibodies at least a year before. The method has been found to be very useful in detecting recent rubella among patients in early pregnancy.

Adolescent↗
PubMed1973

Outbreak in Bristol of conjunctivitis caused by adenovirus type 8, and its epidemiology and control.

In an investigation of an outbreak of adenovirus type 8 infection involving over 40 people it was found that the infection spread initially within the eye hospital, but subsequently involved several family contacts and two local hospitals for mentally subnormal patients. Presumptive diagnosis should be possible before subepithelial opacities have developed provided an adequate history is taken, and had that been done in this outbreak it is reasonable to suppose that many cases could have been prevented.

Adenoviridae↗
PubMed1972

A virus from epidemic vomiting disease.

An illness consisting of vomiting, fever, and mild diarrhoea after a short incubation period was observed in a boarding school and reproduced in volunteers who received filtered extracts of faeces from a typical case. The main discriminatory diagnostic feature of the illness in volunteers was vomiting. The faeces contained no pathogenic bacteria nor any virus that could be detected in tissue cultures and there was no evidence that an infective agent could be grown in organ cultures of human intestine. The agent was shown to be ether stable and passed a 50-nm filter. Laboratory studies on another agent of uncertain significance and obtained in other epidemics are briefly described.

Adolescent↗