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Biomedical subjects

S K Basu

Publications and source records attributed to S K Basu.

151 records · Page 9Linked to original sources
PubMedDate not supplied

Survey of termite-inhabited soil and mosquito breeding sites in Lucknow, India for potential mycopathogens of Anopheles stephensi.

During a short survey of soil and mosquito breeding sites in Lucknow, India for potential mycopathogen from a period of August-October 1996, 11 species of fungi in 5 genera were isolated using live mosquito larvae as host, Aspergillus flavus, A. fumigatus and Fusarium semitectum were the most frequently isolated species. Other fungi recorded were A. niger, A. ochraceus, A. terreus, A. versicolor, Geotrichum candidum, Penicillium verrucosum, Paecilomyces sp. and Fusarium sp. (Liseola/Elegans complex). Insect cell walls are known to contain chitin, so fungal isolates were tested for their chitinase activity on semi synthetic medium containing colloidal chitin. High chitinolytic activities were observed with A. flavus and A. ochraceus. Chitinase producers can be considered as potential pathogens. However, the higher incidence of F. semitectum could not be explained by inability to utilize chitin.

Animals↗
PubMedDate not supplied

Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum.

The nucleotide sequence of a 4.8-kilobase mRNA for hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase, the endoplasmic reticulum enzyme that controls cholesterol biosynthesis, shows that it is a protein of 887 amino acids (molecular weight 97,092) which contains three potential sites for asparagine-linked glycosylation. The reductase is a transmembrane glycoprotein, but in contrast to many other transmembrane glycoproteins, it lacks a cleavable or hydrophobic NH2-terminal signal sequence.

Amino Acid Sequence↗
PubMedDate not supplied

Stimulation of cholesteryl ester synthesis in macrophages by extracts of atherosclerotic human aortas and complexes of albumin/cholesteryl esters.

Cholesteryl ester-rich particles extracted from human atherosclerotic plaques were shown to increase the rate of incorporation of [14C]oleate into cholesteryl [14C]oleate and to cause massive accumulation of cholesteryl esters in monolayers of mouse peritoneal macrophages. This stimulation showed saturation kinetics and susceptibility to competition by polyanions (polyinosinic acid, fucoidin, dextran sulfate), suggesting that cell surface binding was required. Cellular uptake and lysosomal hydrolysis of the cholesteryl esters were also required, as indicated by the finding that stimulation of cholesteryl ester formation was prevented by the lysosomal inhibitor, chloroquine. The cholesterol esterification-stimulating activity of the aortic extracts was excluded on a 2% agarose column and floated in the density range of 1.006 to 1.063 g/ml. Cholesterol-rich extracts from human adrenal glands and liver did not stimulate cholesteryl ester formation in macrophages. The aortic extracts did not stimulate cholesteryl ester synthesis in human fibroblasts. Complexes of 125I-labeled albumin and cholesteryl linoleate formed in vitro were taken up and degraded in macrophages, but not in fibroblasts, by a process resembling the uptake of the aortic extracts. The current data suggest that macrophages express mechanisms for internalizing certain types of cholesteryl ester-rich lipid/protein complexes, including those present in atherosclerotic plaques.

Adult↗
PubMedDate not supplied

Standardisation of methods for the estimation of thiocyanate and iodine in foodstuff.

Thiocyanate content in vegetables has been estimated by passing the water extract of vegetables through an alumina column. The thiocyanate content in the eluate has been measured according to Johnston and Jones. Iodine content in the vegetables has been estimated according to a modified method of Acland. The results show the primarily the Cruciferae family vegetables contain higher concentration of thiocyanate.

Animals↗