Biomedical subjects
Publications and source records attributed to S K Basu.
In Sunderland, acute medical hospital admissions for patients aged 65 and over are cared for primarily by the Consultant Physician in Geriatric Medicine. Many patients have associated surgical problems, and since 1954 these have been investigated and treated in the Surgical/Geriatric wards. This paper examines the type of work encountered and attempts to evaluate the benefits of this combined approach.
Explore the source record for details and available documents.
Radiation induced changes in testicular activity were studied by estimating sialic acid content in plasma and testis and 17-ketosteroids in 24 hr urine samples of male Sprague Dawley rats following 8 Gy whole body gamma ray exposure with and without pretreatment with 2-aminoethylisothiuronium bromide hydrobromide (AET) or with a combination of 5-hydroxy L-tryptophan (5-HTP) and AET. Combination of 5-HTP with AET or AET alone in optimum radioprotecting dose has significantly modified the radiation damage to the testis.
Drug resistant strains of E. coli were freeze dried for long term preservation. Certain drug resistance markers were maintained after freeze drying while others were not. Streptomycin and sulphonamide resistance markers resisted freeze drying. Ampicillin, cephelaxin and neomycin resistances developed very frequently and except gentamicin all the markers were lost in varying percentage after freeze drying.
Tumours arising from large blood vessels are very rare and usually malignant, commonly arising from large veins such as the inferior vena cava. We present two cases of malignant tumours of peripheral vessels.
Forty-nine patients aged 65-89 years, treated for 6 months-6 years (mean 3.9 years) over a total of 195 patient years, were studied. The efficacy of anticoagulant control or the occurrence of complications or treatment failures did not vary with the age, sex, social status, mobility, visual acuity, domiciliary supervision of medication or the indication for anticoagulation. A significant correlation was observed between the concomitant drug therapy and anticoagulant control (P less than 0.001) but not with the occurrence of complications and treatment failures. Poor anticoagulant control was observed particularly in those receiving drugs known to potentiate warfarin effect and in whom more changes were made to their concomitant drug therapy. Five patients who experienced six non-fatal haemorrhages (two major and four minor) showed poor overall anticoagulant control from the outset (P less than 0.01). The treatment failure rate was 4%.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Ninety-two consecutive elderly patients suffering from acute gastrointestinal bleeding were studied over a 3-year period. All were admitted to a specially designated Geriatric/Surgical Ward under the care of consultant physicians in geriatric medicine. The nurses were trained to look after acute surgical and geriatric patients. Patients were aged between 65 and 93 years. All were managed jointly by the surgeons and physicians. Only 13 needed emergency surgery which was preceded by urgent endoscopy. The rest were managed conservatively with intravenous cimetidine and blood transfusions as required. The overall mortality was low at 5.4%. It is concluded that in a series of elderly patients with acute gastrointestinal bleeding the mortality was not high in a unit where the management was conservative and there was joint care between geriatricians and surgeons.
Radiation induced changes in peripheral blood cells were studied in male Swiss albino mice after 4 Gy and 10 Gy whole body gamma irradiation with and without pretreatment with 2-amino-ethylisothiuronium bromide hydrobromide (AET) or with a combination of 5-hydroxy-L-tryptophan (5-HTP) and AET. Combination of 5-HTP with AET or AET alone in a high dose achieved excellent protection against both 4 Gy and 10 Gy whole body irradiation as regards peripheral blood cell counts.
Explore the source record for details and available documents.
The effects of phytohemagglutinin (PHA) stimulation on the activities of sialyltransferase 1 (SAT-1), and sialyltransferase 3 (SAT-3), in human lymphocytes were investigated in vitro. For SAT-1 and SAT-3, respectively, the apparent Km values with variable CMP-NeuAc concentrations were 0.19 and 0.015 mM and with variable LacCer were 0.075 and 0.17 mM. Progressive increases in the activities of SAT-1 and SAT-3 were detected in lymphocytes stimulated with PHA, whereas no increase was observed in control lymphocytes incubated in culture medium alone. These increased activities occurred within 18-36 h of incubation and preceded optimum lymphocyte proliferation. Intact lymphocytes were needed for the lectin-stimulated increase of sialyltransferase activities because neither concanavalin A nor phytohemagglutinin added to the broken cell preparation modulated SAT-1 activity. The glycolipid products formed as a result of these enzymatic reactions in the presence of endogenous and exogenous acceptors were tentatively identified by thin-layer chromatography and autofluorography. The addition of exogenous LacCer to the SAT-1 assay resulted in the radiolabeling of a small amount of ganglioside GM1b (3.4%), but GM3 was the major labeled product (96%). When GgOse4Cer was added to the SAT-3 assay, 32% GM3 and 24.6% GM1b were detected while 44% consisted of glycolipids not labeled in assays performed without exogenous acceptors. Of the radioactivity transferred to endogenous acceptors, 81.3% was in GM3 and 14.6% in GM1b. These results demonstrate that the modulation of sialyltransferase activity occurs earlier than cellular activation.
Explore the source record for details and available documents.
Radioprotecting effectiveness of S-[2-(diethylamino)ethyl] 4-methylbenzothiohydroximate hydrochloride (Dev-B-9) and S-[2-(diethylamino)ethyl]alpha-keto 4-methyl benzothiohydroximate hydrochloride (Dev-B 43) were evaluated by both survival studies in mice exposed to 10.5 Gy whole body gamma-irradiation and 24 hours deoxycytidine (CdR) excretion in urine of rats following 5 Gy irradiation. The drugs were tested after intraperitoneal (i.p.) administration. The drugs rendered significant protection in sublethal (5 Gy) irradiation but could not protect against 10.5 Gy exposure.
The effects of different intraperitoneal doses of MT81 (2.5 mg/kg, 5.0 mg/kg and 7.5 mg/kg, respectively) for a 2-week, 4-week and 6-week treatment on concentration of total lipid and its different fractions of liver, brain, kidney, testis and serum in mice were studied. The following were measured: total lipid, free and total cholesterol, total phospholipid and its fractions (lecithin, lysolecithin, phosphotidylethanolamine and lysophosphotidylethanolamine), triglyceride, free fatty acid; lipase activity was also measured in serum, liver, and testis. Ascorbic acid content of testis was also measured. This mycotoxin (MT81) caused biochemical disorders of the tissues. Total cholesterol, free cholesterol, total phospholipid and its fractions, triglycerides and total lipids were elevated significantly in liver, serum, kidney and testis of toxin-treated animals. Free fatty acid increased significantly at a later stage in serum and remained constant in other tissues of toxin-treated mice. All the lipid fractions except total phospholipid and its fractions remained constant in brain of treated animals; total phospholipid and its fractions decreased significantly in brain of toxin-treated mice. Lipase activity decreased significantly in serum, liver and testis of treated mice. Ascorbic acid content of testis of treated animals increased significantly. Such disorders of lipid concentrations in the aforesaid tissues might be associated with the CNS depressant action and structural and functional toxicity of other tissues induced by the MT81 toxin.
Explore the source record for details and available documents.
Explore the source record for details and available documents.